• Journal of Korean Society of Forest Science
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• v.102 no.1
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• pp.129-135
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• 2013

Japanese larch (Larix leptolepis) has been extensively planted in Korea as a reforestation species but their supply has been a major bottleneck due to sporadic natural seed production. In this study, stem girdling was applied to 32-year-old grafted Japanese larches, resulting in significantly enhanced seed production compared to the controls. Stem girdling induced about 4 times higher cone production than that of controls in the Japanese larch seed orchard. Time-dependent metabolic alterations after girdling were investigated by stable isotope ratio mass spectrometer, HPLC, and GC-MS analysis. In girdled trees, the contents of total nitrogen, sucrose, and total free amino acids were significantly higher than the non-girdled trees at the flowering differentiation season (from July to August). Moreover, the numbers of female strobilus per tree were positively correlated with the contents of both total nitrogen (r=0.765, p<0.01) and total amino acids (r=0.802, p<0.01) in the bark being collected at the flowering differentiation time (August 20). Interestingly, the levels of various individual amino acids at the flowering differentiation times, such as aspartic acid, glutamic acid, glycine, serine, and cysteine, were also significantly correlated (p<0.05) with the numbers of strobilus, suggesting that those amino acids might be involved in the induction of female strobilus formation of Japanese larches.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.1
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• pp.1-6
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• 2005

With recent interest organic farming the use of legumes including vetch and clover to provide N to adjacent crops is increasing in Korea. In the present studies, we conducted a trial to investigate the effects of the application of N rate on nitrogen fixation and transfer from vetch to barley in mixed stands. The experiment was arranged in a randomized complete block design with three replications. Four different N rates(0, 75, 113 and 150/ha) was used and vetch+barley was broadcasted manually on 1.5 $\times$2 m plot in Oct. 2001. Half of urea and K$_{2}O, 200 Phosphate and 75 kg potash per ha were applied as basal dressing md half of N md 75 potash were used for topdressing to soil surface on MarctL 2002. The equivalent of 1kg ha$^{-1}$ at($^{15}$NH$_{4}$)$_{2}$SO$_{4}$ solution at 99.8 atom $\%$$^{15}$N excess was applied to the microplot in mid April. Forage was harvested from each plot at ground level and separated into barley and vetch. Total N content and It values of samples were determined using a continuous flow stable isotope ratio mass spectrometry(IsoPrime-EA. Micromass, UK.). The percentage of legume H fixed from atmospheric N2 were 95.0, 93.8, 94.4 and $84.8\%$ with increment of N levels. The percentage of N transfer from vetch to barley by N-difference method with increment of N fertilizer were from 58 to$49\%$ while 39 to $23\%$ in $^{15}$N-dilution method. The amount of transfer from vetch to barley were 87 to 68 kg/ ha with N level by N-difference moth여 and 58 to -56/ha with N application levels by $^{15}$N dilution method. The amount of nitrogen fixation per ha were from 150 kg / ha to 219 kg / ha by different method, but on the other side 49 to 105kg/ha by N$^{15}$-dilution.

• Analytical Science and Technology
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• v.32 no.1
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• pp.17-23
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• 2019

Sources of NOx are both anthropogenic (e.g. fossil fuel combustion, vehicles, and other industrial processes) and natural (e.g. lightning, biogenic soil processes, and wildfires). The nitrogen stable isotope ratio of NOx has been proposed as an indicator for NOx source partitioning, which would help identify the contributions of various NOx sources. In this study, the ${\delta}^{15}N-NO_2$ values of vehicle emissions were measured in an urban region, to understand the sources and processes that influence the isotopic composition of NOx emissions. The Ogawa passive air sampler was used to determine the isotopic composition of $NO_2$(g). In urban tunnels, the observed $NO_2$ concentration and ${\delta}^{15}N-NO_2$ values averaged $3809{\pm}2656ppbv$ and $7.7{\pm}1.8$‰, respectively. The observed ${\delta}^{15}N-NO_2$ values are associated with slight regional variations in the vehicular $NO_2$ source. Both $NO_2$ concentration and ${\delta}^{15}N-NO_2$ values were significantly higher near the expressway ($965{\pm}125ppbv$ and $5.9{\pm}1.4$‰) than at 1.1 km from the expressway ($372{\pm}96ppbv$ and $-11.5{\pm}2.9$‰), indicating a high proportion of vehicle emissions. Ambient ${\delta}^{15}N-NO_2$ values were used in a binary mixing model to estimate the percentage of the ${\delta}^{15}N-NO_2$ value contributed by vehicular NOx emissions. The calculated percentage of the ${\delta}^{15}N-NO_2$ contribution by vehicles was significantly higher close to the highway, as observed for the $NO_2$ concentration and ${\delta}^{15}N-NO_2$.

• Journal of the Korean Society for Marine Environment & Energy
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• v.12 no.3
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• pp.133-142
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• 2009

In order to understand the present environmental condition and future impingement of Changjiang(Yangtze River) outflow upon the adjacent seas after the scheduled completion of the Sanxia (Three Gorges) Dam in 2009, we tried to estimate the mixing ratios among surface waters of three end-members: Changjiang Water (CW), Kuroshio Water (KW), and East China Sea Water (ECSW) using $^{228}Ra/^{226}Ra$ activity ratio and salinity as tracers. Water samples were collected from 32 stations in November 2005 (R/V Tamgu 3), from 20 stations in July 2006 (R/V Ocean 2000) and from 17 stations in August 2006 (R/V Ieodo) in the northern part of the East China Sea. Radium isotopes in ~300 liters of surface seawater were extracted onboard by filtering through manganese impregnated acrylic fibers and following coprecipitation as $Ba(Ra)SO_4$. Activities of radium isotopes were determined by a high purity germanium detector. Results show that the fraction of CW was in the range of 1-23% in the study area, while KW was in the range of 0-30 % and ECSW 58-100 %. The eastward plume of Changjiang outflow, commonly observed in satellite images during summer and also displayed by the eastward-decreasing CW fraction in this study, could be attributed to Ekman transport caused by the SE monsoon prevailing in this region during summer. Results of this study showed that in the drought season, there was a little or no fraction of CW in the study area. Concentration of dissolved inorganic nitrogen (DIN) showed strong positive relationship with the fraction of CW, suggesting Changjiang as the major source of nitrogen. The mixing curve of DIN indicates the removal of nitrate by biological uptake during the mixing of CW with ambient seawater in the study area.

• Korean Journal of Environmental Biology
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• v.30 no.3
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• pp.210-218
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• 2012

The morphological characteristics, carbon and nitrogen concentrations, stable isotope values and photosynthetic rates of Porphyra yezoensis were studied at the main purple lavers production areas on southwestern coast of Korea. The morphological characteristics of leaf length, leaf width and weight of Porphyra blades were between 11.6~16.3 (average 13.8) cm, 4.6~6.3 (average 5.4) cm, $1.1{\sim}2.6(average\;1.86)g\;DW\;m^{-2}$, respectively. Photosynthetic pigment of Chl a concentration of Porphyra blades was between $2.18{\sim}17.77(average\;9.65)mg\;DW\;Chl\;a\;m^{-2}$. Carbon and nitrogen concentrations of Porphyra blades was between $201{\sim}317(average\;240)mg\;DW\;g^{-1}$, $39.8{\sim}50.0(average\;43.5)mg\;DW\;g^{-1}$ and C/N ratio 5.0~6.7 (average 5.5). The range of average ${\delta}^{13}C$ and ${\delta}^{15}N$ values of Porphyra blades was between - 25.6 to - 24.0 (average - 24.7)‰ for ${\delta}^{13}C$, and 1.3 to 4.1 (average 2.1)‰ for ${\delta}^{15}N$. Photosynthetic characteristics of seaweeds measured by pulse amplitude modulation (PAM) fluorometry was used as an indicator of photosynthetic activity. We use Diving-PAM fluorometry to examine photosynthetic rates of the seaweeds Porphyra yezoensis at each station. Maximum quantum yield of Porphyra blades was between 0.46~0.55 (average 0.52), the variance of the effective PS II maximum quantum yield of the station was broadly similar. Maximum relative electron transport rate (rETRmax) of Porphyra blades was between $4.71{\sim}5.84(average\;5.33){\mu}mol\;electrons\;m^{-2}\;s^{-1}$, the changes of maximum relative electron transport rate (rETRmax) of Porphyra yezoensis were similar to those of PS II maximum quantum yield. Photosynthetic efficiency (${\alpha}$) was between 0.027~0.045 (average 0.036). Minimum saturating irradiance ($E_k$) range was $139{\sim}180(average\;156){\mu}mol\;photons\;m^{-2}\;s^{-1}$. Minimum saturating irradiance ($E_k$) made a difference by station within the area on southwestern coast. Carbon and nitrogen concentrations and photosynthetic rates of Porphyra blades production areas on southwestern coast were broadly similar. The photosynthetic characteristics showed low photosynthetic rates because the low maximum quantum yields and low maximum relative electron transport rate.

• Journal of Soil and Groundwater Environment
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• v.14 no.2
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• pp.1-9
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• 2009

This study was objected to identify the effect on water quality and contamination by non-agricultural facilities in 'A' reservoir watershed located in OO city, Kyounggi-do, Korea. Ground- and stream water samples showed (Na+K)-Cl, Ca(Cl, SO$_4$) and Ca-Cl type in an illegally discharging area of sewage and a densely industrial area indicating water contamination. Stream water of an illegally discharging area of sewage had high COD, T-N and T-P. In this area, direct incoming of sewage into stream water was induced ground water system by well pumping, and it made a progress of ground water contaminations with those components. Groundwater of a densely industrial area showed high concentrations of T-N, NO$_3$N. From a nitrogen isotope analysis, stream water of an illegally discharging area of sewage has ${\delta}^{15}N-NO_3$values of 0.7%0 was strongly affected by nitrogen originated from agrochemicals, and a densely industrial area of 19.7%0 from septic system. Ground- and stream water of a livestock fanning area were contaminated with NH$_3$-N and Mn, which was affected by intensive livestock facilities. SAR-conductivity plot indicates the water does not pose either alkalinity or salinity hazard for irrigation. COD, T-N, T-P, NO$3$-N, NH$_3$N and Mn concentrations from contaminated areas were diminished by mixing with 'A' reservoir water. There were no water contaminations in silver towns, vacationlands around reservoir and golf links. Consequently, it should be made a plan of systematic managements for past and- present possible contaminants and sewage systems in preventing water contamination by non-agricultural facilities.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.21 no.1
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• pp.36-43
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• 2016

Wild glass eels found in the Korean peninsula are thought to migrate along the North Equatorial Current and undergo the processes of spawning and incubation in the Mariana Trench. Juveniles of the wild glass eels are collected from the southern and western coasts of the Korean peninsula and used as seeds for aquaculture. To investigate the feeding behavior of wild glass eels, we collected glass eels from the Nakdong River estuary during March and April 2014 and analyzed the total length, wet weight, dry weight, carbon and nitrogen contents, and stable isotope ratio of eels as well as water temperature and salinity. Water temperature in the Nakdong River estuary was $13.2{\pm}1.0$ (mean ${\pm}$ 1SE; range, $10.1{\sim}15.7)^{\circ}C$ and salinity was $24.8{\pm}2.4$ (13.2~34.0), showed a lower range from 13.2 to 30.0 (a mean of 21.2) when the floodgates were opened. The total length of glass eels was $56.5{\pm}0.2$ (51.0~63.6) mm, wet weight was $70.9{\pm}1.4$ (33.6~133.2) mg, and dry weight was $16.5{\pm}0.3$ (10.1~29.1) mg. Carbon and nitrogen contents of glass eels were $51.0{\pm}0.8%$ and $13.9{\pm}0.1%$, respectively. Mean ${\delta}^{13}C$ and ${\delta}^{15}N$ values of glass eels collected from the Nakdong River estuary were -20.9±0.2‰ and 6.1±0.1‰, displaying similar values to those of leptocephalus, glass eel larvae collected from the North Equatorial Current. Therefore, this result suggest that the glass eels collected from the Nakdong River estuary do not feed on prey after metamorphosis from eel larvae to glass eels their migration.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.12 no.4
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• pp.380-389
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• 2007

Stable carbon and nitrogen isotopes were analyzed in suspended particulate organic matter, macroalgae and macrobenthic invertebrates in order to determine the importance of primary organic matter sources in supporting food webs of rocky subtidal and intertidal macroalgal beds in the Korean coasts. Investigations were conducted at the inter tidal sites within Gwangyang bay, a semi-enclosed and eutrophicated bay, and the subtidal sites of the east coast, a relatively oligotrophic and open environment, in May and June 2005. Water-column suspension feeders showed more negative $\delta^{13}C$ values than those of the other feeding guilds, indicating trophic linkage with phytoplankton and thereby association with pelagic food chains. In contrast, animals of the other feeding guilds, including interface suspension feeders, herbivores, deposit feeders, omnivores and predators, displayed relatively less negative $\delta^{13}C$ values than those of the water-column suspension feeders and similar with that of macroalgae, indicating exclusive use of macroalgae-derived organic matter and association with benthic food chains. Most the macrobenthic species were considered to form strong trophic links with benthic food chains. In addition, the distribution of higher $\delta^{15}N$ values in macrobenthic consumers and macroalgae at the intertidal sites of Gwangyang Bay than those at the subtidal sites of the east coast suggests that anthropogenic nutrients may enhance the macroalgal production at the intertidal sites and in turn be incorporated into the particular littoral food web in Gwangyag Bay. These results confirm the dominant role of macroalgae in supporting rocky subtidal and intertidal food webs in the Korean coasts.

• Korean Journal of Soil Science and Fertilizer
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• v.1 no.1
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• pp.89-115
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• 1968

1. The experiment was carried out for investigating the interaction between potassium nutrition and thermoperiod (as an environment regulating factor) in relation to behaviors of several nutrients including phosphorus-32 and Potassium-42 in IPOMOEA BATAS. 2. To obtain same condition to trace the behaviors of phosphorus and potassum-42 they were simultaneously incorporated to roots. The determination of each CPM by counting twice with adequate interval and calculating true CPM of each isotope according to different half-life, was carried out with satisfactory. 3. Some specific symptoms i.e, chlorosis and withering of growing point under the condition of lower potassium level were found and was accelerated by the low night temperature. 4. A manganese shortage in growing point of the lower potassium level was found by activiation analysis and very low distribution ratio of phosphorus-32 and potassium-42 in the growing point of the lower potassium level was manifested, though the contents of nitrogen, phosphorus, potassium, sodium and magnesium were not in great difference. 5. In addition to the low water content with appearence of "hard", shorterning internode and lower ratio of roots to shoot as well as the symptoms of potassium deficiency such as brown spot in leaf blade and necrosis of leaf margin were appeared at later stage of experiment at the lower potassium level. 6. Very stimulating vegetative growth, e.g, large plant length, leaf expansion, increasing node number and fresh weight as well as high ratio of roots to shoot, high water content was resulted in the condition of higher potassium level. 7. A specific interaction between higher potassium level and thermoperiod was found, that is, the largest tuber production and the largest ratio of roots to shoot were resulted in the combined condition of higher potassium level and constant temperature while the largest plant length, fresh weight etc. i.e. the most stimulative vegetative growth was resulted in the combined condition of higher potassium level and low night temperature. 8. Comparatively low water content in the former condition of stimulative tuber production was resulted(especially at the tuber thickening stage), while high water content in the latter condition of stimulative vegetation was resulted though the higher potassium level made generally high water contents. 9. The nitrogen contents of soluble and insoluble did not make distinct difference between the lower and higher potassium level. 10. Though the phosphorus contents were not distinctly different by the potassium level, the lower potassium level made the percentage of phosphorus increased at tuber forming stage accumulating more phosphorus in roots, while the higher potassium level decreased percentage of phosphorus at that stage. 11. The higher potassium level made distinctly high potassium contents than the lower potassium level and increased contents at the tuber forming stage through both conditions. 12. The sodium contents were low in the condition of higher potassium level than the lower potassium level and decreased at tuber forming stage in both conditions, on the contary of potassium. 13. Except the noticeable deficeney of manganese in the growing point of the lower potassium level, mangense and magnesium contents in other organs did not make distinct difference according to the potassium level. 14. Generally more uptake and large absorption rate of phosphorus-32 and potassium-42 were resulted at the higher potassium level, and the most uptake, and the largest absorption rate of phosphorus and potassium-42 (especially potassium-42 at tuber forming stage) were resulted in the condition of higher potassium level and constant temperature which made the highest tuber production. 15. The higher potassium level stimulated the translocation of phoshorus-32 and potassium-42 from roots to shoots while the lower potassium level suppressed or blocked the translocation. 16. Therefore, very large distribution rate of $p^{32}$, $K^{42}$ in shoot, especially, in growing point, compared with roots was resulted in the higher potassium level. 17. The lower potassium level suppressed the translocation of phosporus-32 from roots to shoot more than that of potassium-42. 18. The uptake of potassium-42 and translocation in IPOMOEA BATATAS were more vivid than phosphorus-32. 19. A specific interaction between potassium nutrition and thermoperiod which resulted the largest tuber production etc. was discussed in relation to behaviors of minerals and potasium-42 etc. 20. Also, the specific effect of the lower and higher potassium level on the growth pattern of IPOMOEA BATATAS were discussed in relation to behaviors of minerals and isotopes. 21. An emphasize on the significance of the higher potassium level as well as the interaction with the regulating factor and problem of potassium level (gradient) for crops product ion were discussed from the point of dynamical and variable function of potassium.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.3
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• pp.119-126
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• 2003

Nitrate-N concentrations and the corresponding ${\delta}^{15}N$ values were determined with water samples collected periodically from artesian wells (3 and 6 m deep), underdrainage and gushout waters in a Welsh onion cultivated area in the Kushibiki Fan, Saitama Prefecture, Japan. Average $NO_3-N$ concentrations in waters from 3 and 6 m wells were 25.7 and $2.8mg\;L^{-1}$, whereas ${\delta}^{15}N$ values were 3.6 and 4.7‰, respectively. The $NO_3-N$ concentration and ${\delta}^{15}N$ value of the underdrainge water were $35.5mg\;L^{-1}$ and 6.6‰, reflecting rapid input of chemical fertilizers and farmyard manure. The mean values of $NO_3-N$ concentration and ${\delta}^{15}N$ in the gushout water flown out of the edge of Kushibiki Fan were $19.4mg\;L^{-1}$ and 7.9‰, respectively. As a results the ${\delta}^{15}N$ values of the gushout water were higher than those of the artesian wells and underdrinage water. The ${\delta}^{15}N$ values of total-N and $NO_3-N$ of the soils were 6.1 and 5.10‰, respectively, while those for nitrification-inhibitor containing fertilizer and slow-release fertilizers were -6.1 and -2.2‰, respectively.