• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.106-106
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• 2022

An appropriate amount of nitrogen fertilizer input during rice cultivation is essential for rice growth, quality control, and reduction of greenhouse gases in paddy fields. Therefore, it is necessary to develop a technology that can check whether an appropriate amount of fertilizer is applied in paddy fields. In this study, we tried to derive a method for diagnosing nitrogen fertilization level using spectroscopic diagnosis, physiological analysis, and molecular indicator genes. Nitrogen fertilization treatment was performed in a greenhouse by dividing into five treatment conditions: no fertilization (N0), low fertilization (N0.5), standard fertilization (N1.0), excessive fertilization (N1.5), and double fertilization (N2.0), respectively. Growth characteristics analysis was investigated by nitrogen fertilization conditions and growth stages, and the height of the canopy was analyzed using a laser scanner. Physiological and spectroscopic analyses were performed by analyzing chlorophyll and sugar contents and measuring SPAD and leaf spectrometer on rice leaves. In addition, real-time PCR experiment was performed to check the relative expression levels of several known nitrogen metabolism related genes. These results suggest that spectroscopic techniques can be helpful in diagnosing the level of nitrogen fertilization in rice paddy fields.

• Korean Journal of Agricultural Science
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• v.50 no.2
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• pp.181-192
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• 2023

Appropriate fertilization methods are required according to species to supply necessary nutrients to plants and prevent soil environmental contamination in nurseries. In this study, the effects of nitrogen and phosphorus fertilization on the growth of Liriodendron tulipifera and soil characteristics were investigated. After 16 fertilization treatments (4 levels of nitrogen × 4 levels of phosphorus) were applied to one-year-old L. tulipifera seedlings at the Yongmun Nursery Station of the Korea Forest Service, height, root collar diameter (RCD), biomass, leaf nutrients, and soil characteristics were investigated. The height increased as the amount of nitrogen and phosphorus fertilization increased, and the RCD was the highest in the ×2 treatment. Biomass growth was on average 40.0% higher for the treatment with high nitrogen fertilization compared to the low nitrogen treatment. The seedling quality index was the highest with nitrogen and phosphorus ×2 treatment. Leaf phosphorus and magnesium concentrations decreased when nitrogen fertilization was applied, and leaf potassium concentrations decreased as nitrogen fertilization increased. Soil pH and exchangeable potassium decreased as the amount of phosphorus application increased, and exchangeable magnesium decreased as the amount of nitrogen application increased and increased as the amount of phosphorus application increased. Considering the growth of L. tulipifera seedlings and changes in the soil characteristics at the nursery stage, twice the standard fertilization amount is the appropriate fertilization amount for nursery of the Yongmun Nursery Station. It is expected that this study will contribute to improving nursery soil fertilization management technology for healthy seedling production.

• Korean Journal of Agricultural Science
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• v.46 no.3
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• pp.629-636
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• 2019

This study was conducted to investigate the growth characteristics and yield of whole green rice grains during the ripening period. These were investigated using Hopumbyeo and Unkwangbyeo at two transplanting times and with two kinds of nitrogen fertilization. The transplanting times were May 30 and June 20, respectively, using 30-day seedling culture and transplanting conducted with 3 - 4 plants per hill in planting space of $15cm{\times}30cm$. During nitrogen fertilization, 9 kg and 18 kg was used, respectively. The harvest of the green whole rice grains was carried out on the 15th, 20th, 25th, 30th, and 40th day after the heading date. The clum length was greater with later planting and with application of more nitrogen. The rice yield was higher with nitrogen fertilization of 18 kg/10 a when transplanted on May 30 for Hupumbyeo, and for Unkwangbyeo, was higher at 9 kg/10 a nitrogen fertilization when transplanted on May 30. The protein content of Hopumbyeo was higher when the nitrogen fertilizer was 18 kg/10 a, and that of Unkwangbyeo was lower than that when transplanting on June 20. The greenness was not related to the nitrogen fertilization level when transplanted on May 20 but for later transplanting, the greenness was higher when the nitrogen application was increased, and the greenness was the greatest about 30 days after the heading date.

• Asian Journal of Turfgrass Science
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• v.4 no.2
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• pp.133-144
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• 1990

This experiment was carried out to study the effect of pure nitrogen fertilization on botanical composition of grassland in 1987~ 1988 under practical conditions at the " Federal Institute for Agriculture in the Alps" in Austria. The application rates were 0, 30, 60, 90 and 120kg/ha/cut, the cutting regimes 3-, 4 , 5- and 6-cuts/year. The results were as follows: 1. Only PK fertilization resulted in higher botanical composition of Trifolium repens among legumes in grassland, which was increased by cutting frequency .2. Nitrogen fertilization on three-cut areas resulted in higher existence of generally valuable tall grasses such as Arrhenotherum elatius Trisetum flavescens and Dactylis glomerata . On the other hand, nitrogen fertilization on four-, five- and six-cut areas showed mainly Doctylis glomerato and Poa pratensis appearance. 3.For some cases, appearance of less valuable grasses, herbs and weeds such as Agropyron repens, Poa trivialis, Poa annua, Setoria viridis, Aegopodium podagra rio, Men landrium rubrum, Taraxacum officlnale, Achillea millefolium, Rorippa sylvestris and Polygonum ocleulare was *Bundesanstalt fur alpenlandische Landwirtschaft Gumpenstein(A-8952 Irdning, 6sterreich) increased on medium and high rates of N fertilized areas. 4.Reduction of sward density may also diminish the advantages of nitrogen fertilization and may be threatened by mainly high dressings of nitrogen combined with too late utilization of the sward. 5.Location altered the efficiency of nitrogen fertilization on botanical composition.mposition.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.5
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• pp.445-450
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• 2006

The nitrogen fertilization effect on growth characteristics and isoflavone content was investigated in this study, and isoflavone analyzed by HPLC with photodiode array (PDA) detector and reverse-phase $C_{18}$ column. Fertilization levels were no-fertilization, no nitrogen, 50% decreased in nitrogen, standard and 50% increased in nitrogen fertilization. The 50% increased nitrogen fertilization showed the highest growth characteristics then other fertilization level and the number of pod and seed showed maximum value 31.9 and 72.3, respectively, and seed yield was 2,460 kg/ha. During growth stages, isoflavone content in leaf, stem and root of soybean plants decreased to R5 stage then increased to R7 stage. Isoflavone content according to various nitrogen fertilization condition, in case of none fertilization, no nitrogen, 50% decreased nitrogen fertilization showed higher value than those of standard and 50% increased nitrogen fertilization levels. Aglycon content among the isoflavone isomers showed much higher in plant than in seed. The highest isoflavone content was found in the root of soybean plant parts. Isoflavone content of seed was higher in none, no nitrogen, 50% decreased nitrogen than those of standard and 50% increased nitrogen fertilization.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.6
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• pp.677-681
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• 2016

Excessive nitrogen fertilization influences crop yields and quality as well as environmental pollution. In this study, yields, nitrogen use efficiency and free sugar contents of burdock (Arctium lappa L.) were evaluated at different levels of nitrogen fertilization. Nitrogen fertilizer was applied at 5 levels (0, 50, 100, 150, 200%) based on the conventional fertilization ($N=230kg\;ha^{-1}$), and phosphate and potassium fertilizer were treated by conventional P and K fertilization ($P_2O_5-K_2O=140-210kg\;ha^{-1}$) in all plots. The root yields of burdock were the highest in N 100~150% treatment plots. Nitrogen use efficiency and nitrogen recovery decreased from over N 150% treatment. Nitrogen uptake of root was greater than that of shoot in N 50~200% treatments. Fructose contents in root were inversely proportional to the level of nitrogen fertilization. As considering nitrogen recovery and root quality, economical burdock yield was obtained in N $230kg\;ha^{-1}$.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.2
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• pp.123-134
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• 1997

The effects of cutting 6equency and nitrogen fertilization on feed values of reed canarygrass were investigated in uncultivated rice paddy and the attemp was made to estimate the economical and efficient levels of nitrogen fertilization for the increased production of nutrients in reed canarygrass. The results are summarized as follows; 1. Crude protein(CP) content was higher at 1st cut of all treatments(12.5% in 3 cutting 6equency; 19, 7% in 4 cutting frequency; 22.5% in 5 cutting kquency). However, Total Digestible Nutrients(TDN) and Relative Feed Value(RFV) in 3 and 4 cutting 6equencies were higher at 3rd cut(63.5%, 126.8) and 4th cut(65.7%, 146.4) respectively in which the contents of Neutral Detergent Fiber(NDF) and Acid Detergent Fiber(ADF) were lowest. In 5 cutting frequency, 1st cut showed the highest TDN and RFV as 70.5% and 232.4 respectively. 2. The level of nitrogen fertilization affected CP content of reed canarygrass. Nitrogen fertilization of 120kgl halcut showed high CP contents of 11.4, 14.7 and 16.5% in 3, 4 and 5 cutting eequency respectively. In 3 and 4 cutting frequency, TDN and RFV were highest with nitrogen fertilization of 90kg/ha/cut where NDF and ADF were lowest, however those in 5 cutting frequency were highest with nitrogen fertilization of 30kg/ha/cut. 3. The yields of CP varied according to the number of cutting frequency and those were 0.45~0.65 in 3 cutting hquency, 0.35~0.50 in 4 cutting frequency, and 0.19~0.67tond/ha in 5 cutting 6equency respectively and TDN was 2.09~3.60, 1.64~2.77 and 0.73~2.96tons/ha respectively. 4. The ratios between the yields of CP and TDN were 5.48, 4.60 and 4.34 in 3, 4 and 5 cutting 6equency respectively and it indicates lower ratio in result of higher number of cutting 6equency. 5. Higher nitrogen fertilization increased the yields of CP and TDN. The yields of CP were higher in 5 cutting frequency as 1.55~2.62tons/ha than of 3(1.20~1.91tons/ha) and 4 cutting frequency(1.35-2.23tons/ha). The yields of TDN were 6.31~9.93, 5.99~9.99 and 7.16~10.57tons/ha in 3, 4 and 5 cutting frequency respectively. 6. The levels of nitrogen fertilization for high production efficiency of crude protein(kg CPYkg N) was estimated as 113.4~302.5kg/ha and it resulted in 1.44~2.09ton/ha of CP yields. The levels of nitrogen fertilization for high production efficiency of dry matter was 199.2-361.3kgha in which nitrogen were fertilized below 400kg/ha and it resulted in 1.64~2.17ton/ha of CP yields. 7. The levels of nitrogen fertilization for high production efficiency of total digestible nutrients(kg TDNYkg N) were 149.9~294.1kg/ha in 3 and 4 cutting kequency and it resulted in 8.66~8.89ton/ha of TDN yileds. With the levels of nitrogen fertilization for high production efficiency of dry matter of 199.2~361.3kg/ha, TDN yields were high as 8.77~9.94ton/ha.

• Journal of Korean Society of Forest Science
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• v.98 no.5
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• pp.558-562
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• 2009

The growth, carbon and nitrogen status of container grown black pine (Pinus thunbergii) seedlings were examined at various levels of foliar fertilization (control, 0.1%, 0.2%, 0.3%). Root collar diameter, height and dry weight of black pine seedlings increased significantly with increasing levels of foliar fertilization (P<0.05). Carbon concentration in needle of black pine seedlings was significantly higher in the foliar fertilization than in the control treatments (P<0.05), while other seedling components such as stem and roots were not significantly different (P>0.05) between the foliar fertilization and the control treatments. Nitrogen concentration and content were significantly greater in the foliar fertilization than in the control treatments (P<0.05). Shoot/root ratio of black pine seedlings (needle+stem dry weight/root dry weight) was greater in the foliar fertilization (2.40-2.89) than in the control treatments (1.87). However, nitrogen use efficiency was significantly lower (P<0.05) in the foliar fertilization (28-46) than in the control (111) treatments. The results indicate that morphological characteristics and nutritional status on container grown black pine seedlings were enhanced by various levels of foliar fertilization.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.69-75
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• 1994

This experiment was canied out to study the effect of cutting frequencies (3, 4 and 5 cuts per year) and mineral nitrogen fertilization on dry matter yields, in order to estimate optimum level of fertilizing mineral nitrogen in orchardgrass(Dacry1is glomerafa L.). The results are as follows: 1. Dry matter yields were the highest in the 1st cut at 3 cutting frequency and in the 2nd cut at 4 and 5 cutting frequencies, and they shared 49.7, 37.0 and 37.0% of annual dry matter yield respectively. 2. When only phosphorus and potassium were applied, annual dry matter yields were between 9.4 and 11.5 tons per ha and the highest yield was observed at 3 cutting frequency. 3. Dry matter yield in relation to fertilization of mineral nitrogen was highly increased when more nitrogen was applied as 360 kg per ha compared to 240 kg per ha at 3 cutting frequency. At 4 and 5 cutting frequencies, increases in dry matter yield, to large extent, were showed at 120 and 150 kg of nitrogen per ha(30 kg Nlcutlha) compared to no application of nitrogen. The efficiencies of mineral nitrogen fertilization (kg DM/kg N) were 28.0, 22.7 and 20.6 kg dry matter yields per nitrogen(kg) respectively). 4. At 4 and 5 cutting frequencies, dry matter yields were reduced when 360 kg and 300 kg of mineral nitrogen per ha(90 kg and 60 kg Nlcutlha) were applied respectively. 5. Particularly at 4 and 5 cutting frequencies of this study, maximum marginal yields reached at 129.9 kg and 148.0 kg of fertilizing mineral nitrogen per ha, and economical borden of mineral nitrogen fertilization were between 222.4 and 250.0 kg and between 244.8 and 276.8 kg respectively. At the same cutting frequencies, the highest dry matter yields were obtained at 365.4 and 433.8 kg of fertilizing mineral nitrogen respectively.

• Journal of Climate Change Research
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• v.6 no.3
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• pp.249-256
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• 2015

This study was conducted to investigate the effects of different levels and types of nitrogen fertilizer on seedlings and soil chemical properties in a semi-arid area, Mongolia. 2-year-old Populus sibirica and 4-year-old Ulmus pumila seedlings were planted in May 2014. Six treatments with three levels of nitrogen (low-level: urea $5g\;tree^{-1}$; medium-level: urea $15g\;tree^{-1}$, ammonium sulfate $33g\;tree^{-1}$, urea $15g\;tree^{-1}$ with potassium phosphate $10g\;tree^{-1}$; high-level: urea $30g\;tree^{-1}$) were applied and for the medium-level of nitrogen, different types of fertilizer were treated. Survival rate, root collar diameter (RCD) growth rate, leaf nitrogen concentration of seedlings, and soil chemical properties were determined in August 2014. The seedling survival rate of both species decreased as the level of nitrogen increased. This result can be explained by water stress caused by nitrogen fertilization in arid regions. The RCD growth rate of P. sibirica was significantly decreased by the treatment of high-level of nitrogen due to excessive nitrogen fertilization, and was increased by the treatment of ammonium sulfate due to sulfur which might promote nitrogen uptake. The leaf nitrogen concentration of P. sibirica did not change by the treatment of low-level of nitrogen, and was increased by the treatment of medium-level of nitrogen. There were no significant differences in the RCD growth rate and the leaf nitrogen concentration of U. pumila among the six treatments. None of soil chemical properties was affected by nitrogen fertilization. Overall, the low-level of nitrogen showed no effect on seedlings and soil chemical properties, except on survival rate of U. pumila and the high-level of nitrogen was considered excessive fertilization. Continuous monitoring of medium-level nitrogen fertilization including the ammonium sulfate, which increased early growth of seedlings, would be needed to elucidate the effect of fertilization on seedling growth and soil properties in a semi-arid region.