• 한국응용곤충학회지
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• 제17권1호
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• pp.33-35
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• 1978

모자익병징을 나타내는 이병시금치를 채집하여 순무모자익 바이러스를 분류 동정하였다. 분리된 순무모자익바이러스를 지표식물에 접종한 결과 담배(B.Y)와 명아주에는 국부병반이 형성되었고 쑥갓, 시금치, 무우에는 모자익 병징이 나타났다. 이병시금치 잎을 착즙하여 순무모자익바이러스의 항혈청과의 혈청반응 시험 결과 양성 반응이 나타났다. 이병엽을 Dip법으로 시료를 제작하여 전자현미경에서 검경한 결과 대부분이 750nm의 사상 입자가 관찰되었다. 시금치에서 순무모자익바이러스의 발생분포는 수원, 안양, 대구, 진주 등 거의 전국적으로 발생하였다

• 한국응용곤충학회지
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• 제17권1호
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• pp.29-31
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• 1978

순수분리된 오이모자익 바이러스(CMV)를 함배 (KY-57)에서 증식하여 개량된 순화방법으로 순화하였다. 순화된 바이러스를 Complete adjuvant와 1: 1로 섞어 토끼에 10일 간격으로 2회 근육주사하여 높은 역가의 CMV 항혈청을 제조하였다. 한천내확산법에 의한 항혈청의 역가는 1/1280이었다. 제조된 CMV 항혈청은 앞으로 채소작물이나 그밖에 식량작물에 이병된 CMV를 분리동정하는데 이용될 것이다.

• Journal of Plant Biology
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• 제29권1호
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• pp.1-10
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• 1986

Intra- and inter-specific protoplast fusion of tobacco (Nicotiana tabacum cv. Virginia 115) and pea (Pisum sativum cv. Sparkle) were carried out in highly inhomogeneous alternating electric fields. Under the electric field of alternating current (AC, sine wave), 600 V/cm and 800 kHz for tobacco protoplast, and 600 V/cm and 700 kHz for pea protoplasts, the protoplasts were aggregated in pearl chains. Intra-specific protoplast fusions were most effectively induced within the aggregates of tobacco and pea, respectively, by the additional application of a single high field pulse of direct current (DC, square wave) at 1 kV/cm for 50 $mutextrm{s}$. Inter-specific fusions between protoplasts of the two plants were most effectively induced in the electric field of 600 V/cm and 700 kHz, and square wave pulse at 1 kV/cm for 50 $mutextrm{s}$. The duration of the pulse over the electrical breakdown voltages was simulated from 1 to 100 $mutextrm{s}$ in both tobacco and pea protoplast. The yield of the electrofusion products was significantly high (above 60%), compared with that (20%) of the standard fusion method by polyethylene glycol (PEG) 4,000, and the viability of electrofused protoplasts was above 70%, but that of PEG-fused protoplasts 8~16%, when determined by Evan's blue staining method.

• Journal of Plant Biology
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• 제36권1호
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• pp.113-120
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• 1993

Effects of various NaCl concentrations on salt-tolerant callus of tobacco were investigated. Selection of NaCl-tolerant (S) callus was conducted by subculturing Nicotiana tabacum cv. BY 4 callus in 200 mM NaCl-containing MS medium for more than 18 months. In spite of the long subculture period, characteristics of salt tolerance were maintained very stably. Significant differences were found in ion contents of each callus which was subcultured with treatment of various NaCl concentrations: Na+ and Cl- became higher but Mg2+, Ca2+ and K+ became lower with the increasing external salt contents. Therefore, the ratios of Na+/Ca2+ and Na+/K+ also increased resulting close to those of halophytic property. The contents of chlorophylls and carotenoids in S callus were estimated to 3.1 and 2.9 times more, respectively. than those of non-selected (NS) callus (control). The higher content of external NaCl tended to increase the amount of water soluble proteins and to decrease the amounts of the total sugars, reducing sugars and free amino acids. The activity of peroxidase was increased with higher contents of external NaCl in S callus, but it was maintained at a higher level than S callus at lower NaCl, followed by a subsequent decrease above 80 mM NaCl in NS callus. These results suggest that S callus may have a biological system converting energy source to efficient growth leading to reduction of the growth inhibition under stress environment.

• The Plant Pathology Journal
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• 제23권1호
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• pp.34-36
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• 2007

Introduction of vegetatively propagated Petunia hybrids since 1992 led to increasing virus infections of propagation material. Petunia hybrid 'Surfinia' cultivated for pot-plant showed yellowing symptom along with stunt. Flowers were smaller in size and showed color-break symptom. Tobacco mosaic virus(TMV-pet) was isolated from the diseased petunia. Healthy petunia plants inoculated with TMV-pet induced mottle on leaves and color-break on flowers, and plants were stunted. Nucleotide sequences of coat protein gene amplified from RNA prepared from Nicotiana tabacum cv. Samsun infected with TMV-pet were determined(GenBank accession no. DQ981481). It showed 99.0% nucleotide sequence homology with TMV-potato3-2(GenBank accession no. AF318215) isolated from potato showing yellow mosaic and stunt symptom, and with a TMV Korean strain(GenBank accession no. X68110). This is the first reported observation of TMV from vegetatively propagated petunia in Korea.

• 한국연초학회지
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• 제17권1호
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• pp.20-26
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• 1995

This study was carried out to obtain the basic data which include the change of the photosynthetic rate and protein content according to growth stage in the process of senescence of tobacco plant The photosynthetic rate was the maximum with 26.31$\mu$mol.CO2/m2.sec and stomatal resistance was the minimum with 0.2552cm/sec at 15th days after leaf emergence. However, after 50 days the photosynthesis was very little occurred. During leaf developments the number of chloroplast was increased and reached at the maximum at 25th days after emergence of leaf, thereafter, it was decreased gradually. The content of protein increased continuously and showed the highest value at 15th days after leaf emergence. The degradation rate of soluble protein was more rapid than that of insoluble protein at early stage of senescence. The range of decrement in the insoluble protein was low at late stage of senescence. The content of Rubisco, the key enzyme of photoamthesis, corresponded to about 50% of soluble protein and reached to the maximum at 150 days after leaf emergence. As the senescence progressed, the content of large subunit(UV) of Rubisco showed a tendency to decrease more rapidly than that of small subunit(SSU). The total amount of amino acids was the highest at 15th days after leaf emergence.

• Plant Biotechnology Reports
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• 제5권4호
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• pp.301-308
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• 2011

This study tested the relative and combined efficacy of ShPx2 and ShPAL transgenes by comparing Nicotiana tabacum hybrids with enhanced levels of $_L$-phenylalanine ammonia lyase (PAL) activity and cationic peroxidase (Prx) activity with transgenic parental lines that overexpress either transgene. The PAL/Prx hybrids expressed both transgenes driven by the 35S CaMV promoter, and leaf PAL and Prx enzyme activities were similar to those of the relevant transgenic parent and seven- to tenfold higher than nontransgenic controls. Lignin levels in the PAL/Prx hybrids were higher than the PAL parent and nontransgenic controls, but not significantly higher than the Prx parent. All transgenic plants showed increased resistance to the necrotrophs Phytophthora parasitica pv. nicotianae and Cercospora nicotianae compared to nontransgenic controls, with a preponderance of smaller lesion categories produced in Prx-expressing lines. However, the PAL/Prx hybrids showed no significant increase in resistance to either pathogen relative to the Prx parental line. These data indicate that, in tobacco, the PAL and Prx transgenes do not act additively in disease resistance. Stacking with Prx did not prevent a visible growth inhibition from PAL overexpression. Practical use of ShPAL will likely require more sophisticated developmental control, and we conclude that ShPx2 is a preferred candidate for development as a resistance transgene.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.65.1-65
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• 2003

In addition to the five well-characterized genes of Tobacco mosaic virus (TMV), this virus contains a sixth open reading frame (ORF6) that encodes a 4.8 kDa protein. TMV ORF6 overlaps the ORFs encoding the 30 kDa movement protein and the adjacent 17.5 kDa capsid protein. Although the 4.8 kDa protein could not be detected in vivo, alteration of the AUG codons of this ORF resulted in a mutant virus that attenuated the virulence of the mutated TMV in Nicotiana benthamiana, but not N. tabacum (tobacco). These sequence changes did not affect either the replication or movement of the mutated TMV. Expression of TMV ORF6 from the virus expression vector Potato virus X (PVX) intensified the virulence of this virus in N. benthmiana, but not tobacco, while expression of TMV ORF6 from the virus expression vector Tobacco rattle virus enhanced the pathogenicity observed in both N. benthamima and tobacco. Thus, the TMV ORF6 is a host- and virus-specific. virulence factor. However, two separate assays indicated that the TMV 4.8 kDa protein was not a suppression of RNA silencing. A fusion protein formed between the TMV 4.8 kDa protein and the green fluorescent protein was expressed from the PVX vector and localized to plasmodesmata. Possible roles of the 4.8 kDa protein in pathogenicity will be discussed

• 한국작물학회지
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• 제28권2호
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• pp.258-261
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• 1983

본 시험은 발아가능한 담배종자를 조기 파종하여 파종에 이용하고자 몇가지 버어리종 담배를 공시하여 수분후 8일부터 30일까지 2일간격으로 채종 발아조사를 하였던 바 1. 종자는 수분후 12일 이상이면 발아가능하여 파종에 이용할 수 있었고 발아율은 낮았으며 품종에 따라 변이가 컸다. 2. 적기채종시기는 수분후 24-28일이였다. 3. 과숙종자는 후숙이나 일시휴면현상을 보여 발아율이 감소하였다.

• Genomics & Informatics
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• 제20권4호
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• pp.43.1-43.18
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• 2022

Atropa belladonna is a valuable medicinal plant and a commercial source of tropane alkaloids, which are frequently utilized in therapeutic practice. In this study, bioinformatic methodologies were used to examine the pattern of coding sequences and the factors that might influence codon usage bias in the chloroplast genome of Atropa belladonna and other nightshade genomes. The chloroplast engineering being a promising field in modern biotechnology, the characterization of chloroplast genome is very important. The results revealed that the chloroplast genomes of Nicotiana tabacum, Solanum lycopersicum, Capsicum frutescens, Datura stramonium, Lyciumbarbarum, Solanum melongena, and Solanum tuberosum exhibited comparable codon usage patterns. In these chloroplast genomes, we observed a weak codon usage bias. According to the correspondence analysis, the genesis of the codon use bias in these chloroplast genes might be explained by natural selection, directed mutational pressure, and other factors. GC12 and GC3S were shown to have no meaningful relationship. Further research revealed that natural selection primarily shaped the codon usage in A. belladonna and other nightshade genomes for translational efficiency. The sequencing properties of these chloroplast genomes were also investigated by investing the occurrences of palindromes and inverted repeats, which would be useful for future research on medicinal plants.