• Journal of Microbiology and Biotechnology
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• v.33 no.2
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• pp.167-179
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• 2023

The rifampicin-resistant strain E9-302 of Edwardsiella ictaluri strain 669 (WT) was generated by continuous passage on BHI agar plates containing increasing concentrations of rifampicin. E9-302 was attenuated significantly by 119 times to zebrafish Danio rerio compared to WT in terms of the 50% lethal dose (LD₅₀). Zebrafish vaccinated with E9-302 via intraperitoneal (IP) injection at a dose of 1 × 10³ CFU/fish had relative percentage survival (RPS) rates of 85.7% when challenged with wild-type E. ictaluri via IP 14 days post-vaccination (dpv). After 14 days of primary vaccination with E9-302 via immersion (IM) at a dose of 4 × 10⁷ CFU/ml, a booster IM vaccination with E9-302 at a dose of 2 × 10⁷ CFU/ml exhibited 65.2% RPS against challenge with wild-type E. ictaluri via IP 7 days later. These results indicated that the rifampicin-resistant attenuated strain E9-302 had potential as a live vaccine against E. ictaluri infection. A previously unreported amino acid site change at position 142 of the RNA polymerase (RNAP) β subunit encoded by the gene rpoB associated with rifampicin resistance was identified. Analysis of the whole-genome sequencing results revealed multiple missense mutations in the virulence-related genes esrB and sspH2 in E9-302 compared with WT, and a 189 bp mismatch in one gene, whose coding product was highly homologous to glycosyltransferase family 39 protein. This study preliminarily explored the molecular mechanism underlying the virulence attenuation of rifampicin-resistant strain E9-302 and provided a new target for the subsequent study of the pathogenic mechanism of E. ictaluri.

• The Journal of the Acoustical Society of Korea
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• v.29 no.8
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• pp.504-508
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• 2010

In conventional diagnostic ultrasound strain imaging, when displaying strain image on a monitor, human visual characteristics are utilized such that hard regions are displayed as dark and soft regions are displayed as bright. Thus, hard regions representing tumor or cancer are displayed as dark, decreasing the contrast inside the lesion. Because the lesion area is stiff and thus displayed as dark, a method of inverting the image brightness and thereby increasing the contrast in the lesion for better diagnostic purposes is proposed wherein a postcompression signal is extended in the time domain by a factor corresponding to the reciprocal of the amount of the applied compression using a technique termed globally uniform stretching. Experiments were carried out to verify the proposed method on an ultrasound elasticity phantom with radio-frequency data acquired from a diagnostic ultrasound clinical scanner. It is found that the new method improves the contrast-to-noise ratio by a factor of up to about 1.8 compared to a conventional strain imaging method that employs a reversed gray color map without globally uniform stretching.

• Journal of Microbiology and Biotechnology
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• v.33 no.11
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• pp.1448-1456
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• 2023

A Gram-positive, non-motile, and non-spore-forming lactic acid bacterium, designated as BK2^T, was isolated from kimchi, a Korean traditional fermented vegetable food, and the taxonomic characteristics of strain BK2^T, along with strain LMG 11983, were analyzed. Both strains optimally grew at 30℃, pH 7.0, and 1.0% NaCl. Cells of both strains were heterofermentative and facultatively anaerobic rods, demonstrating negative reactions for catalase and oxidase. Major fatty acids (>10%) identified in both strains were C_18:1 ω9c, C_16:0, and summed feature 7 (comprising C_19:1 ω6c and/or C_19:1 ω7c). The genomic DNA G+C contents of both strains were 44.7 mol%. The 16S rRNA gene sequence similarity (99.9%), average nucleotide identity (ANI; 99.9%), and digital DNA-DNA hybridization (dDDH; 99.7%) value between strains BK2^T and LMG 11983 indicated that they are different strains of the same species. Strain BK2^T was most closely related to Weissella confusa JCM 1093^T and Weissella cibaria LMG 17699^T, with 100% and 99.4% 16S rRNA gene sequence similarities, respectively. However, based on the ANI and dDDH values (92.3% and 48.1% with W. confusa, and 78.4% and 23.5% with W. cibaria), it was evident that strain BK2^T represents a distinct species separate from W. confusa and W. cibaria. Based on phylogenetic, phenotypic, and chemotaxonomic features, strains BK2^T and LMG 11983 represent a novel species of the genus Weissella, for which the name Weissella fermenti sp. nov. is proposed. The type of strain is BK2^T (=KACC 22833^T=JCM 35750^T).

• Journal of Microbiology and Biotechnology
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• v.34 no.5
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• pp.1040-1050
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• 2024

To isolate and analyze bacteria with Verticillium wilt-resistant properties from the fermentation residue of kitchen wastes, as well as explore their potential for new applications of the residue. A total of six bacterial strains exhibiting Verticillium wilt-resistant capabilities were isolated from the biogas residue of kitchen waste fermentation. Using a polyphasic approach, strain ZL6, which displayed the highest antagonistic activity against cotton Verticillium wilt, was identified as belonging to the Pseudomonas aeruginosa. Bioassay results demonstrated that this strain possessed robust antagonistic abilities, effectively inhibiting V. dahliae spore germination and mycelial growth. Furthermore, P. aeruginosa ZL6 exhibited high temperature resistance (42℃), nitrogen fixation, and phosphorus removal activities. Pot experiments revealed that P. aeruginosa ZL6 fermentation broth treatment achieved a 47.72% biological control effect compared to the control group. Through activity tracking and protein mass spectrometry identification, a neutral metalloproteinase (Nml) was hypothesized as the main virulence factor. The mutant strain ZL6ߡNml exhibited a significant reduction in its ability to inhibit cotton Verticillium wilt compared to the strain P. aeruginosa ZL6. While the inhibitory activities could be partially restored by a complementation of nml gene in the mutant strain ZL6CMߡNml. This research provides a theoretical foundation for the future development and application of biogas residue as biocontrol agents against Verticillium wilt and as biological preservatives for agricultural products. Additionally, this study presents a novel approach for mitigating the substantial amount of biogas residue generated from kitchen waste fermentation.

• Journal of Microbiology and Biotechnology
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• v.17 no.8
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• pp.1254-1261
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• 2007

In the present study, we have constructed a bioluminescent bioreporter for the assessment of nitrate/nitrite bioavailability in wastewater. Specifically, an approximately 500-bp DNA fragment containing a nitrate/nitrite-activated nasR-like promoter (regulating expression of genes encoding nitrite reductase in the genus Klebsiella) was fused upstream of the Vibrio fischeri luxCDABE gene cassette in a modified mini-Tn5 vector. Characterization of this strain, designated W6-1, yielded dose-dependent increased bioluminescence coincident with increased nitrate, nitrite, and ammonium added to the growth medium from 1 to 11 ppm. Bioluminescence in response to nitrogen species addition was light dependent up to 10, 7, and 8 ppm with nitrate, nitrite, and ammonium, respectively. This response was linear in the range from 1 to 8 ppm for nitrate ($R^2=0.98$), 1 to 6 ppm for nitrite ($R^2=0.99$), and 1 to 7 ppm for ammonium ($R^2=0.99$). A significant bioluminescent response was also recorded when strain W6-1 was incubated with slurries from aged, nitrate/nitrite contaminated wastewater. Thus, bioreporter strain W6-1 can be used to elucidate factors that constrain the use of nitrate/nitrite in wastewaters.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.23 no.4
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• pp.353-360
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• 2010

In this paper, an efficient algorithm is established in order to estimate the plastic properties of power-law hardening bulk specimen materials with one simple spherical indentation impression test. This work is based on a new formulation of representative strain and, therefore, compare to the preceding approaches the fitting parameters are significantly reduced. Moreover, the new definition of representative strain endowed more physical meaning to the representative strain. In order to verify the reliability of the reverse analysis, we have studied a broad set of materials whose property ranges cover essentially all engineering metals and alloys. Based on the indentation force-displacement P-${\delta}$ curves obtained from numerical simulations, the characteristics of the indentation response and material elastoplastic properties are bridged via explicit functions. Next, through the procedure of reverse analysis the yield stress and power-law hardening exponent of bulk specimen materials can be determined. Finally, good agreement between the result from reverse analysis and initial input data from experiment can be observed.

• Geomechanics and Engineering
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• v.15 no.5
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• pp.1081-1089
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• 2018

The deformation and strength of brittle rocks are significantly influenced by the crack closure behavior. The relationship between the strength and deformation of rocks under uniaxial loading is the foundation for design and assessment of such scenarios. The concept of relative crack closure strain was proposed to describe the influence of the crack closure behavior on the deformation and strength of rocks. Considering the crack compaction effect, a new damage constitutive model was developed based on accumulated AE counts. First, a damage variable based on the accumulated AE counts was introduced, and the damage evolution equations for the four types of brittle rocks were then derived. Second, a compaction coefficient was proposed to describe the compaction degree and a correction factor was proposed to correct the error in the effective elastic modulus instead of the elastic modulus of the rock without new damage. Finally, the compaction coefficient and correction factor were used to modify the damage constitutive model obtained using the Lemaitre strain equivalence hypothesis. The fitted results of the models were then compared with the experimental data. The results showed that the uniaxial compressive strength and effective elastic modulus decrease with an increase in the relative crack closure strain. The values of the damage variables increase exponentially with strains. The modified damage constitutive equation can be used to more accurately describe the compressive deformation (particularly the compaction stage) of the four types of brittle rocks, with a coefficient of determination greater than 0.9.

• Journal of Food Hygiene and Safety
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• v.5 no.4
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• pp.187-196
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• 1990

This study was carried out to develop new model for gastric ulcer and to investigate some factor which effect it. Rats were immersed for 8 hours. Ulcer index was measured from total length of occured ulcer. Ulcer index (UI) of restraint and immersion group was higher significantly than that of only restraint group (p<0.001) and ulcer was occurred within only glandular stomach. UI of low water temperature group was higher significantly than that of high water temperature group (p

• Steel and Composite Structures
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• v.28 no.5
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• pp.589-606
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• 2018

The previous studies reflected the significant effect of neutral-axis position and coupling of in-plane and out-of-plane displacements on behavior of functionally graded (FG) nanobeams. In thin FG beam, this coupling can be eliminated by a proper choice of the reference axis. In shear deformable FG nanobeam, not only this coupling can't be eliminated but also the position of neutral-axis is dependent on through-thickness distribution of shear strain. For the first time, in this paper it is avoided to guess a shear strain shape function and the exact shape function and consequently the exact position of neutral axis for arbitrary gradation of higher order nanobeam are obtained. This paper presents new methodology based on differential transform and collocation methods to solve coupled partial differential equations of motion without any simplifications. Using exact position of neutral axis and higher order beam kinematics as well as satisfying equilibrium equations and traction-free conditions without shear correction factor requirement yields to better results in comparison to the previously published results in literature. The classical rule of mixture and Mori-Tanaka homogenization scheme are considered. The Eringen's nonlocal continuum theory is applied to capture the small scale effects. For the first time, the dependency of exact position of neutral axis on length to thickness ratio is investigated. The effects of small scale, length to thickness ratio, Poisson's ratio, inhomogeneity of materials and various end conditions on vibration and buckling of local and nonlocal FG beams are investigated. Moreover, the effect of axial load on natural frequencies of the first modes is examined. After degeneration of the governing equations, the exact new formulas for homogeneous nanobeams are computed.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.32 no.4
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• pp.18-23
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• 2004

In this paper, material non-linear behavior of PZT wafer(3203HD, CTS) under high electric field and stress is experimentally investigated and the non-linearity of the PZT wafer is numerically simulated. Empirical functions that can represent the non-linear behavior of the PZT wafer have been extracted based on the measured piezo-strain under stress. The functions are implemented in an incremental finite element formulation for material non-linear analysis. New definition of the piezoelectric constant and the incremental strain are incorporated into the finite element formulation for a better reproduction of the non-linear behavior. With the new definition of the in incremental piero-strain the measured non-linear behavior of the PZT wafer has been accurately reproduced even for high electric field. For validation of the measured non-linear characteristics and the proposed approach, a PZT bimorph beam actuator has been numerically and experimentally tested. The predicted actuation displacement, based on the material nonlinear finite element analysis, showed a good agreement with the measured one.