• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.88-94
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• 2008

Two sugar biosynthetic cassette plasm ids were used to direct the biosynthesis of a deoxyaminosugar. The pOTBP1 plasmid containing TDP-glucose synthase (desIII), TDP-glucose-4,6-dehydratase (desIV), and glycosyltransferase (desVII/desVIII) was constructed and transformed into S. venezuelae YJ003, a strain in which the entire gene cluster of desosamine biosynthesis is deleted. The expression plasmid pOTBP3 containing 4-aminotransferase (gerB) and 3,5-epimerase (orf9) was transformed again into S. venezuelae YJ003-OTBP1 to obtain S. venezuelae YJ003-OTBP3 for the production of 4-amino-4,6-dideoxy-L-glucose derivatives. The crude extracts obtained from S. venezuelae ATCC 15439, S. venezuelae YJ003, and S. venezuelae YJ003-OTBP3 were further analyzed by TLC, bioassay, HPLC, ESI/MS, LC/MS, and MS/MS. The results of our study clearly shows that S. venezuelae YJ003-OTBP3 constructs other new hybrid macrolide derivatives including 4-amino-4,6-dideoxy-L-glycosylated YC-17 (3, [M+ $Na^+$] m/z=464.5), methymycin (4, m/z=480.5), novamethymycin (6, m/z=496.5), and pikromycin (5, m/z=536.5) from a 12-membered ring aglycon (10-deoxymethynolide, 1) and a 14-membered ring aglycon (narbonolide, 2). These results suggest a successful engineering of a deoxysugar pathway to generate novel hybrid macrolide derivatives, including deoxyaminosugar.

• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1546-1554
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• 2010

A bacterial strain capable of producing extracellular ${\alpha}$-galactosidase was isolated from a sample of sugarcane industrial waste. Microbiological, physiological, and biochemical studies revealed that the isolate belonged to Bacillus sp. Furthermore, based on a 16S rDNA sequence analysis, the new isolate was identified as Bacillus megaterium VHM1. The production of ${\alpha}$-galactosidase was optimized based on various physical culture conditions. Guar gum and yeast extract acted as the best carbon and nitrogen sources, respectively. The optimum pH was 7.5 and the enzyme remained stable over a pH range of 5-9. The enzyme was optimally active at $55^{\circ}C$ and thermostable with a half-life of 120 min, yet lost 90% of its residual activity within 120 min at $60^{\circ}C$. One mM concentrations of $Ag^2$, $Cu^2$, and $Hg^{2+}$ strongly inhibited the ${\alpha}$-galactosidase, whereas the metal ions $Fe^2$, $Mn^{2+}$, and $Mg^{2+}$ had no effect on the ${\alpha}$-galactosidase activity, and $Zn^{2+}$, $Ni^{2+}$, and $Ca^{2+}$ reduced the enzyme activity slightly. When treated with the B. megaterium VHM1 enzyme, the flatulence-causing sugars in soymilk were completely hydrolyzed within 1.5 h.

• 한국치위생학회지
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• 제18권5호
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• pp.843-852
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• 2018

Objectives: The study aimed to isolate the abundant bacteria in dental caries in children and to investigate the bacterial species involved in addition to those that have been previously reported. Methods: The specimens were collected from the supragingival plaques of each dental caries area, pit and fissure caries, deep dentinal caries, smooth surface caries, and dental caries, and from healthy subjects in the control group. Bacteria were cultured from these specimens, DNA was extracted from the isolated bacteria, and the 16S rRNA gene sequences were analyzed and identified. Results: Based on the results of the 16S rRNA gene sequence analysis for the 90 strains of dominant bacteria from the 45 specimens, 5, 7, 8, 7, and 13 species were identified from the supragingival plaques from healthy teeth, pit and fissure caries, deep dentinal caries, smooth surface caries, and dental caries, respectively. In healthy teeth, Actinomyces naeslundii dominated. Corynebacterium durum, Ralstonia pickettii, and Streptococcus intermedius showed equal distribution. The dominant bacterial species in dental caries, S. sanguinis, showed the greatest difference in prevalence in pit and fissure caries. In deep dentinal caries, S. mutans and Lactobacillus rhamnosus were dominant; in smooth surface caries, S. mutans and S. sanguinis were dominant; and in the supragingival plaques of dental caries, S. sanguinis and S. mutans were dominant. Conclusions: The bacterial species isolated from dental caries encompassed four phyla, eight genera, and 22 species. In addition, the SS1-2 strain, belonging to the genus Neisseria, was identified as a new species from among the isolated strains.

• 한국가축번식학회지
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• 제15권3호
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• pp.179-187
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• 1991

This experiment was carried out to develop a new technique of identifying XX of XY-bearing bisected embryos prior to implantation by immunological method. H-Y antiserum prepared in inbred Wastar female rats by repeated immunization with spleen cells from males of the same strain. The reactivity of H-Y antibody was confirmed by culturing mouse embryos in the medium containing H-Y antiserum and complement obtained from the guinea pig. The optimal condition for the activity of H-Y antibody was also investigated by culturing embryos under the concentraton or affected H-Y antibody was also investigated by culturing embryos under the concentration or affected H-Y antibody and culture rate. However, production of live young or sex rates of male and female from embryos transferred with psudopregnant. The biological test with the morula stage embryos showed that H-Y antibody was formed in all female rats immunized with spleen cell, but it was formed only in 80% female rats immunized with the antigen. When the bisected mouse embryos were cultured in vitro for 5~6 hours in morula stage, of 457 bisected embryos 81.4% of then were developed to the blastocyst stage. When the concentration rate of complement to H-Y antiserum varied from 1.0~5.0${mu}ell$, the lysis-rate of embryo was 19.5 to 67.3%. The concentration rate of complement did not influence the lysis-rate of embryos(P<0.05). The morphology embryos of bisected, zona-free and intact embryos showed the embryos lysis rate of 58.6, 42.7 and 48.5% respectively(P<0.05). Pregnancy rate were 50.0, 45.5 and 57.1% in psudopregnant recipient transferred with bisected, zona-free and intact blastocyst embryos. However, production of live youngs, sexual rate of male or female was 24(50.0:50.0), 22(45.5:55.5) and 36(58.3:41.7)mice, but affected and non affected half embryos with H-Y antiserum treatment was 23.1 and 26.7%. Also production of live youngs and sexual rate was 14(92.9:7.1) and 17(17.6:82.4)mice in affected and non affected half embryos in H-Y antiserum treatment(P<0.05).

• The Plant Pathology Journal
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• 제34권4호
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• pp.269-285
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• 2018

Bacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rademarker's group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov.

• 화약ㆍ발파
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• 제37권4호
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• pp.1-9
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• 2019

본 연구에서는 지하채광 발파에 따른 암반광주에서의 변위 및 진동을 측정하는 방법을 통해 디지털 이미지 영상상관 기법(DIC)이라는 기술의 적용성을 시험하였다. 디지털 이미지 영상상관 기법은 고속 사진촬영 기술과 접목하면 구조물 또는 대상물의 전면변화 속성을 2차원 또는 3차원 좌표로 변환할 수 있는 우수한 광학영상 처리능력을 제공한다. 이렇게 측정된 좌표들은 구조물이나 대상물의 변위, 변형률, 속도 등을 계산하는 데 사용된다. 이 기술은 과학 및 공학 분야에서 널리 사용되고 있으며, 그 밖의 많은 분야로도 적용성을 확장해 나가고 있다. 본 연구에서는 이와 같은 DIC 시스템과 기존의 진동계측기를 사용하여 발파로 발생된 변위와 진동을 측정함으로써 그 성능을 서로 비교하였다. 현장시험 결과, 두 방법은 비슷한 결과를 보였다. 이로부터 DIC 시스템을 이용한 발파 진동 및 변위 측정에 대한 적용성을 확인할 수 있었다.

• 한국강구조학회 논문집
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• 제19권1호
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• pp.43-52
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• 2007

CFT(Concrete Filled Steel Tube, 콘크리트 충전 강관) 부재는 뛰어난 구조거동으로 인하여 그 사용 영역이 점차 확대되고 있는 추세이나, 대단면의 부재로 사용될 경우에는 자중과 경제성에서 불리할 수 있다. 본 연구에서는 CFT 부재의 단면을 중공으로 만듦으로써, 콘크리트 사용량을 절감하여 부재의 자중을 감소시키고, 중공면에 강관 또는 FRP 튜브를 삽입하여 성능을 향상시킨 내부 구속 중공 CFT 부재의 합리적인 해석을 위하여 비선형 콘크리트 모델을 개발하였다. 개발된 비선형 콘크리트 모델은 구속효과를 고려한 Mander의 일축 압축 콘크리트 모델을 기본으로 하였으며, 내부 구속 중공 CFT 부재 내 콘크리트의 응력-변형율 선도를 결정하기 위해서, 내부 구속 중공 CFT 부재의 발생 가능한 파괴 형상을 제시하고, 각각의 파괴 형상이 발생할 때의 구속응력을 평형방정식을 통하여 유도하였다. 유도된 식을 통하여, 컴퓨터를 이용한 해석 프로그램이 작성되었으며, 매개변수 연구가 수행되었다. 해석결과, 내부 구속된 콘크리트는 비구속 상태 또는 이축 구속 상태의 콘크리트에 비하여, 향상된 강도와 연성을 갖는다는 것을 확인하였다.

• 대한바이러스학회지
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• 제30권1호
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• pp.19-28
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• 2000

Two distinct hantaviruses have been isolated from Apodemus agrarius in 1976 and Rattus norvegicus in 1980 in Korea. Since our serosurveys conducted in 1994, a genetically distinct hantavirus from Apodemus peninsulae has been investigated. To isolate hantavirus from Apen insulae captured in Korea, the lung homogenate of seropositive Apeninsulae inoculated Vero E6 cells. Viral antigen was detected in a progressively higher percentage of cells with subsequent passage after 80 days postinoculation. The new isolate from seropositive Apodemus peninsulae was designated Suchong virus after Suchong valley located in northeastern region of South Korea. Comparing with hantaan virus 76-118 strain, Suchong virus-1, 2, 3 and 4 showed the similarity of $71.0{\sim}91.8%$ at nucleotide and $90.9{\sim}94.8%$ at amino acid sequences in 231 nucleotides region of M segment, and the similarity of $75.1{\sim}81.0%$ at nucleotide and $97.5{\sim}100%$ at amino acid sequences in 237 nucleotides of S segment.

• Parasites, Hosts and Diseases
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• 제37권4호
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• pp.265-270
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• 1999

The gene encoding Plasmodium vivax circumsporozoite protein (PvCSP) exhibits polymorphism in many geographical isolates. The present study was designed to investigate polymorphism in PvCSP gene of P. vivax isolates in Korea. Thirty isolates, obtained from indigenous cases in Yonchon-gun, Kyonggi-do in 1997, were subjected for sequencing and RFLP analysis of the repeat and post-repeat regions of PvCSP gene and two genotypes (SK-A and SK-B) were identified. The genotype of 19 isolates was SK-A and that of 11 isolates was SK-B. Although the number of 12-base repeats present in SK-A was three while two were found in a Chinese strain CH-5, the repeat sequence of SK-A was identical to that of CH-5 except for one base substitution. Compared with known data there was no identical isolates with SK-B, but the sequence of SK-B was similar to that of a North Korean (NK) isolate. These results indicate that two genotypes of PvCSP coexist in the present epidemic area of Korea and the present parasite may originate from East Asia. RFLP would be useful to classify genotypes of P. vivax population instead of gene sequencing.

• 비파괴검사학회지
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• 제26권2호
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• pp.122-129
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• 2006

본 논문에서는 레이저 응용 비파괴검사 기술인 Shearography를 이용하여 압력 배관 내부 결함의 검출에 있어서 미치는 전단량의 영향을 분석하였다. Shearography는 레이저 응용기법에 비하여 환경 외란에 둔감하며, 대상체 표면의 상대 변위를 검출함으로서 비파괴검사 분야에서 많은 활용이 기대된다. 현재까지 연구는 적용 가능성의 평가와 적용 대상의 확대에 중점을 두어 연구가 진행되어 왔으며, 결함의 정량평가를 위해서는 결함 검출에 있어서 영향을 주는 인자들에 대한 연구가 필요하다. 따라서 본 논문에서는 결함의 검출에 영향을 줄 수 있는 전단량의 변화에 따라 결함의 크기 측정과 길함의 위치 결정에 대한 영향을 예측하고, 압력용기 내부의 길이 방향 인공 결함에 대해 적용하여 검출 결과를 분석하였다.