• Korean Journal of Agricultural Science
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• v.19 no.1
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• pp.16-27
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• 1992

To determine a potential of new sludge fertilizer for horticultural crops, comparative studies between commercial fertilizers (Jandibiryo and Wonyebokbi) and sludge fertilizer (Sludgebiryo) were made through examining the growth responses on zoysiagrass (Zoysia japponica Steud.) and several horticultural plants. 1. The pH of new sludge fertilizer remained near 6.5 regardless the particle size. The solubility of elements was highest in phosphorus, followed by nitrogen and potassium in the order. Especially, desorption of potassium was continued up to 48 hrs after solubilization. 2. There was an increase in shoot number per plant, length of stolon and rhizome, and root weight as well as clipping yield of zoysiagrass in the treatment of large size Sludgebiryo compared to small one and Jandibiryo. 3. Regardless the size of fertilizers, Sludgebiryo increased flower numbers in salvia (Salvia officinalis L. 'Hatzazz') compared to Wonyebokbi, although the difference was not great, However, leaf area and fresh weight of plant were more increased in Wonyebokbi application. 4. Flower diameter of marigold (Tagetes erecta L. 'Inca') was slightly increased in Sludgebiryo application, but the average number of lateral shoots and fresh weight per plant were significantly increased in the treatment of Wonyebokbi application. 5. Sludgebiryo effectively increased the length of both main and lateral shoots, number of flowers and weight of shoot in vinca (Vinca rosea L. 'Little Linde'), but root growth of plant was higher in Wonyebokbi application. 6, No differences between Wonyebokbi and Sludgebiryo were found in promoting the growth of leaves of perilla (Perilla frutescens Hara 'Yubsil' ), but chlorophyll content and seed weight were slightly higher in the application of Wonyebokbi compared to Sludgebiryo. In conclusion, the effectiveness of Sludgebiryo for horticultural plants was almost equal to commercial fertilizers such as Jandibiryo or Wonyebokbi. Expecially, Sludgebiryo appeared to effective on the growth of zoysiagrass, and the increase of flower size and numbers in flower crops. Results indicate that new-made Sludgebiryo can be recommended for turfgrass culture, and the flower crops in which quality depends on flower number and flower size.

• Weed & Turfgrass Science
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• v.2 no.1
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• pp.23-29
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• 2013

This study was conducted to clarify effects of weed control methods on damages from agricultural chemicals of pear trees, growth of weeds and states of pear trees after treating pear orchards with different methods of weed controlling, density of GLU and application times. The growth and occupation rate were investigated after 10, 20 and 40 days of weeding treatment. According to a result of the first treatment conducted when weeds in the lower parts of crown in a pear orchard began to grow and grew about 20 cm, unwoven cloth covering showed the highest control value with 100% in all 14 kinds of grasses. In comparison, Stellaria aquatica and catchweed bedstraw showed 96.7% and 97.3% respectively in the 20 DAT investigate after the first treatment of GLU 540 g a.i. ha-1 and the high control value of 100% in other all kinds of grasses. According to an investigation of stalk enlargement, length of new shoot and the number of new shoot made to know differences in tree growth following treatment of the lower part of crown, to use weed killers two or three times a year or to eliminate grasses with machines have positive effects on cross growth of pear trees.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.1
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• pp.41-48
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• 1992

This experiment was carrid out to determine the effect of cutting height and nitrogen level on dead stubble, tiller and branch new shoot dry matter yield and total dry matter of sorghum-sudangrass hybrid{Sorghum bicolor (L.) Moench}.The main treatment was four cutting height(5cm, IOcm, 15cm, 20cm), and the sub treatment was three nitrogen level(l50kg, 250kg, 350kglha).The experiment was performed at the Collage of Natural Science of Kon-Kuk University at Chungju in 1988. 1. Plant length tends to increase in the high cutting height and high N level. Especially, the difference of plant height was significant in warm season. 2. The number of dead stubble after cutting was significantly(P

• Journal of Sericultural and Entomological Science
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• no.12
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• pp.1-4
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• 1970

The experimental studies were intended to clarify the effects of leaf yield calculations, and also aimed at estimating the correlations between some characters for the selection of desirable stocks of mulberry trees. The analysis of covariances was the variance components procedures in a replicated trial for the varieties, and also it was used to obtain the genotypic, phenotypic and environmental correlations between the eight characters-branch length, node number, branch diameter, branch number per stock, total branch weight, old branch weight, new shoot and leaf weight, and leaf weight, etc. The results obtained are summarized as follows: Phenotypic, genotypic and environmental covariances are shown in Table 1 and phenotypic correlations, genotypic correlations and environmental correlations are shown in Table 2. Genotypic correlations were slightly higher than the corresponding phenotypic correlations between the characters in the varieties, but the variation in values due to the change of environment appeared between the characters. Genotypic correlations between the leaf yield of mulberry trees and other characters indicated that high leaf yield was genetically correlated with six characters namely branch length, node number, branch number per stock, total branch weight, old branch weight and new shoot weight, etc. It was also observed that lower correlations were appeared between the leaf yield and branch diameter, and branch diameter and other all characters studied in both genotypic and phenotypic correlation coefficients.

• Plant Resources
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• v.5 no.1
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• pp.29-44
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• 2002

In this study, plant regeneration through in vitro culture from plantlet stems of Yooja (C. junos Sieb.) and trifoliate orange (P. trifoliata Rafin.) was attempted to make mass-production system of virus-free plants having the same genotype with mother plant. In order to investigate physiological change depending on the developmental stage of plant regeneration, the changes of total protein, peroxidase and esterase activity and their isozyme patterns as well were examined in 1/2 MS medium. The results are as follows : 1. The MS medium for the optimal callus induction and shoot formation was utilized. The medium was supplemented either with 2,4-D and Kinetin or with BA and NAA. The optimal concentrations were the combination of 1.0mg/ 2,4-D +0.3mg/ Kinetin and 1.0mg BA +0.3mg NAA in callus induction and shoot formation, respectively. 2. For the plant regeneration from somatic embryos, 1/2 MS medium was used with supplements of growth regulators (free, 1.0mg/ IBA +1.0mg/ BA ,0.5mg/ IBA +0.5mg/ BA). Shooting and rooting were the best in the treatment of 0.5mg/ IBA and 0.5mg/ BA combination. 3. The total protein content has a tendency of increase with the developmental stage of embryo, but it was decreased at the plantlet. Also it was the highest at 8 and 6 weeks stage in C. junos Sieb. and P. trioliata Rafin, respectively. In the SDS-PAGE pattern of protein, C. junos Sieb. showed bands of 29.0 and 40kDa at 10 weeks. The 45,66 and 97.4 kDa bands at 10 weeks of culture were shown in P. trifoliata Rafin. 4. The highest esterase activity was shown at the 6 and 8 weeks of culture in C.junos Sieb. and P. trifoliata Rafin.., respectively. 5. Esterase isozyme patterns were shown difference according to the developmental stage. In C. junos Sieb. a new band was observed at pl 7.7 following 4 weeks culture. On the other hand, new bands in P. trifoliata Rafin. were observed at pl 7.5~6.5 following 4 and 6 weeks culture, respectively.

• Korean Journal of Plant Resources
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• v.24 no.2
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• pp.121-129
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• 2011

As the molecular breeding was progressed, many plant transformation techniques were attained for improving transformation accuracy and used to produce useful transgenic plants. Day by day, new varieties were developed so new transformation techniques required for these newly developed varieties. Carnation (Dianthus caryophyllus L.) is a popular and economically important ornamental plant, all over the world. Keeping this in view, we selected 18 varieties of D. caryophyllus L. commonly available in the market and did optimization of culture conditions for more efficient tissue culture and to get higher number of plants via micro-propagation. Four varieties namely Yellowdotcom, Jakarta, Belmonte, Polartessino etc. were selected for organ culture studies from single cell line. The optimum growth was recorded in the MS media supplied with sucrose 3%, NAA 1.0 mg/L and TDZ 1.0 mg/L. except Belmonte, in which, BA 1.0 mg/L was found to be the best combination, in place of TDZ, rest ingredients were same. The most efficient coagulating agent used to obtain higher number of plant from callus was phytagel 0.3%. The most effective explant for higher shoot formation was stem in which 80.2% shoot formation was recorded. It also reduced culture periods by 6 days.

• Journal of Bio-Environment Control
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• v.25 no.3
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• pp.168-172
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• 2016

The effect of different exposed length of M.9 rootstock on growth and yield was tested in the new apple cultivars. The 'Seohong', 'Summer Dream', and 'Hongguem' grafted on M.9 rootstock were planted in March 2010. The trees were planted in such a way that the exposed length of the rootstock to be 10, 20 and 30 cm. The result showed that as decreasing exposure length, TCA of 'Seohong', 'Summer Dream', and 'Hongguem' was increased between $3^{rd}$ and $6^{th}$ year after planting. The TCA of 20 cm and 30 cm exposure length showed respectively 70% and 60% in 'Seohong', 88% and 66% in 'Summer Dream', and 55% and 41% in 'Hongguem' of the TCA with 10 cm exposure length on 6-year-old trees. Tree height, canopy width, shoot length and terminal shoot length were also increased according to decreased exposure length in 6-year-old trees. The cumulative yield of three cultivars was higher in the tree with a lower exposure length between $4^{th}$ and $6^{th}$ year after planting. The cumulative yield of 20 cm and 30 cm exposure length showed respectively 77% and 63% in 'Seohong', 85% and 76% in 'Summer Dream', and 73% and 58% in 'Hongguem' of the cumulative yield with 10 cm exposure length in 6-year-old trees. Considering tree growth and yield, the optimum exposure length of M.9 was 10 cm for 'Seohong', 'Summer Dream' and 'Hongguem'.

• Korean Journal of Plant Tissue Culture
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• v.25 no.3
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• pp.207-217
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• 1998

Total of 78 strains were characterized based on the morphological characteristics of colonies isolated on Schroth, and New & Kerr's media for selection of hypervirulent wild-type Agrobacterium spp from galls, hairy root-like process and soil of Populus, Malus, Salix and Diopyros in Korea. Among them, 48 strains were able to induce tumors in carrot disc. Hypervirulent A. tumefaciens SP101 and SM042 were identified as biotype 1 and biotype 2, respectively, These strains formed fast growing, larger tumors as compared to those induced by other strains. The binary vector pGA643 with kanamycin resistant gene was mobilized from E. coli MC100 into A. tumefaciens strain SM042 isolated from soil, and/or disarmed vector PC2760 using a triparental mating method with E. coli HB101/pRK2013, and transconjugants, A. tumefaciens SM643 and PC643 were obtained in minimal media containing kanamycin and tetracycline. Tobacco tissues were cocultivated with conjugant Agrobacterium and then transferred to selective medium with 2,4-D and kanamycin to induce the transformants. Calli were formed more efficiently in cocultivation with A. tumefaciens SM643 than that with A. tumefaciens PC643. Most of calli transformed with A. tumefaciens PC643 were friable and regenerated into normal plantlets, while the calli transformed with A. tumefaciens SM643 were compact, hard, and mixed with friable calli. The friable calli formed normal shoots, while compact calli did not form shoots but only grew to typical compact tumor calli. When the shoots formed directly from tobacco stems without callus induction after transformation by A. tumefaciens SM643 with wild-type Ti-plasmid, normal transformed plants can be induced without using disarmed Ti-plasmid.

• Journal of Climate Change Research
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• v.7 no.1
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• pp.9-17
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• 2016

Climate change affects plant responses on physiological characteristics and growth, and Pinus densiflora, one of the major tree species in Korea, are expected to be particularly vulnerable to rising temperature and increased precipitation. This study was conducted to investigate the effects of an open-field warming and precipitation manipulation on physiological characteristics and growth of P. densiflora seedlings. Seedlings of 2-year-old P. densiflora were planted in April, 2013, in open-field nursery located at Korea University. The air temperature of warmed plots had been set to be $3^{\circ}C$ higher than the control plots using infrared lamps. Precipitation was manipulated to be 30% lower or higher than the control, using transparent panels and drip irrigation. Net photosynthetic rate, total chlorophyll content, seedling height, root collar diameter and biomass were measured from April, 2014 to April, 2015. The increase in new shoot biomass from warming was statistically significant, with the biomass in warmed plots about 2-fold higher than in the control plots in 2014 and 2015. This result might be related to advanced bud burst and increased occurrence of abnormal new shoots in warmed plots. Meanwhile, the results of net photosynthetic rate, total chlorophyll content, seedling height, root collar diameter and total biomass from warming and precipitation manipulation were not statistically significant, but tendencies of lower net photosynthetic rate and higher seedling height and biomass in warmed plots compared to the control were shown. Such might be speculated as results of the extended growth period. When root to shoot (R/S) ratio was calculated from the biomass data obtained in April 2014 and April 2015, increased R/S ratio was observed regardless of the treatments applied. Drought tolerance of P. densiflora and particularly low annual precipitation observed in 2014 were suggested as the possible reasons.

• Journal of Korean Society of Forest Science
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• v.87 no.2
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• pp.164-172
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• 1998

This study was conducted to find the optimum transformation condition using Agrobacterium harboring promoterless GUS gene. The optimal medium for shoot induction from leaves of Populus nigra${\times}$P. maximowiczii was MS medium supplemented with $0.1mg/{\ell}$ NAA, $0.5mg/{\ell}$ BAP(94% regeneration frequency and 11.5 average number of shoot) According to the test using pBI121, the concentration of antibiotics for selection marker gene was $100mg/{\ell}$ kanamycin or $60mg/{\ell}$ geneticin in the SIM(shoot inducing medium) 3. Two weeks later, callus was induced in the SIM 3 and this callus grew up to 0.5-1cm shoots after 6 weeks in the new SIM 3. And the treatment with methylation inhibitor(5-azacytidine) led to a dramatic increase in foreign gene expression rate from 5.7% to 26.7%. The vector systems showed. different transformation efficiencies based on the fluorometric and histochemical GUS assay. In this study the vector systems used for transformation seemed to affect transformation frequency, in which pEHA101 yielded more transformants(35.9%) than LBA4404/pBI121 did(5.7%). This result indicated that pEHA101 was effective to insert the promoterless foreign gene into a poplar genome.