• Title/Summary/Keyword: new approach

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Pyramiding transgenes for potato tuber moth resistance in potato

  • Meiyalaghan, Sathiyamoorthy;Pringle, Julie M.;Barrell, Philippa J.;Jacobs, Jeanne M.E.;Conner, Anthony J.
    • Plant Biotechnology Reports
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    • v.4 no.4
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    • pp.293-301
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    • 2010
  • The feasibility of two strategies for transgene pyramiding using Agrobacterium-mediated transformation was investigated to develop a transgenic potato (Solanum tuberosum L. cv. Iwa) with resistance to potato tuber moth (PTM) (Phthorimaea operculella (Zeller)). In the first approach, cry1Ac9 and cry9Aa2 genes were introduced simultaneously using a kanamycin (nptII) selectable marker gene. The second approach involved the sequential introduction (re-transformation) of a cry1Ac9 gene, using a hygromycin resistance (hpt) selectable marker gene, into an existing line transgenic for a cry9Aa2 gene and a kanamycin resistance (nptII) selectable marker gene. Multiplex polymerase chain reaction (PCR) confirmed the presence of the specific selectable marker gene and both cry genes in all regenerated lines. The relative steady-state level of the cry gene transcripts in leaves was quantified in all regenerated lines by real-time PCR analysis. Re-transformation proved to be a flexible approach to effectively pyramid genes for PTM resistance in potato, since it allowed the second gene to be added to a line that was previously identified as having a high level of resistance. Larval growth of PTM was significantly inhibited on excised greenhouse-grown leaves in all transgenic lines, although no lines expressing both cry genes exhibited any greater resistance to PTM larvae over that previously observed for the individual genes. It is anticipated that these lines will permit more durable resistance by delaying the opportunities for PTM adaptation to the individual cry genes.

The Application and Development Method of Construction Lines for Conceptions of Clothes Design (의상디자인 발상을 위한 구성선의 응용과 전개방법)

  • Kong, Mi-Ran
    • Fashion & Textile Research Journal
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    • v.10 no.6
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    • pp.848-856
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    • 2008
  • In today's world where a preference for prestigious brands prevails, it's essential to have creative design conceptions rather than try simple mimicking or following in order to be recognized for creative and objective design works. In that sense, the development of clothes design using construction lines offers a great approach towards the ideas of reinterpreting and creating new designs since it creates new forms by understanding lines as signs of conception and transforming them. That approach allows a designer to improve his or her imagination, creativity and expression in a freer and richer manner, think of many original ideas through diverse changing processes, and develop the skill to visualize the ideas by incorporating geometric forms of abstract lines into clothes. When approaching the conception of clothes design from the standpoint of construction lines, you need to adopt more specific and systematic design methods instead of vague imagination to introduce simple facts or theories to a new design process. That's one of the ways you can approach clothes design easily and enhance your conception skills. The process of clothes design according to the conception of construction lines is composed of the following stages; the preparation stage involves an understanding of the principles and the entire process of clothes design conception based on construction lines; the idea stage requires a designer to make free alterations of the given basic construction lines by moving and replacing the lines and sides in diverse ways; in the creation and expression stage, the designer uses the newly reconstructed construction lines to create clothes design; and in the evaluation stage, the designer makes a presentation of his or her subjective design works and has his or her works assessed and tested in an objective fashion. In today's modern society that demands new and original globalized design, the results of the study will help to invigorate the development of new fashion design in a more systematic and practical manner by suggesting a feasible and gradual educational program for the development of design based on the technique of construction lines.

New approaches to testing and evaluating the impact capability of coal seam with hard roof and/or floor in coal mines

  • Tan, Y.L.;Liu, X.S.;Shen, B.;Ning, J.G.;Gu, Q.H.
    • Geomechanics and Engineering
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    • v.14 no.4
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    • pp.367-376
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    • 2018
  • Samples composed of coal and rock show different mechanical properties of the pure coal or rock mass. For the same coal seam with different surrounding rocks, the frequency and intensity of rock burst can be significantly different in. First, a method of measuring the strain variation of coal in the coal-rock combined sample was proposed. Second, laboratory tests have been conducted to investigate the influences of rock lithologies, combined forms and coal-rock height ratios on the deformation and failure characteristics of the coal section using this method. Third, a new bursting liability index named combined coal-rock impact energy speed index (CRIES) was proposed. This index considers not only the time effect of energy, but also the influence of surrounding rocks. At last, a new approach considering the influences of roof and/or floor was proposed to evaluate the impact capability of coal seam. Results show that the strength and elastic modulus of coal section increase significantly with the coal-rock height ratio decreasing. In addition, the values of bursting liability indexes of the same coal seam vary greatly when using the new approach. This study not only provides a new approach to measuring the strain of the coal section in coal-rock combined sample, but also improves the evaluation system for evaluating the impact capability of coal.

A Market-Based Replacement Cost Approach to Technology Valuation (기술가치평가를 위한 시장대체원가 접근법)

  • Kang, Pilsung;Geum, Youngjung;Park, Hyun-Woo;Kim, Sang-Gook;Sung, Tae-Eung;Lee, Hakyeon
    • Journal of Korean Institute of Industrial Engineers
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    • v.41 no.2
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    • pp.150-161
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    • 2015
  • This paper proposes a new approach to technology valuation, the market-replacement cost approach which integrates the cost-based approach and market-based approach. The proposed approach estimates the market-replacement cost of a target technology using R&D costs of similar R&D projects previously conducted. Similar R&D projects are extracted from project database based on document similarity between project proposals and technology description of the target technology. R&D costs of similar R&D projects are adjusted by mirroring the rate of technological obsolescence and inflation. Market-replacement cost of the technology is then derived by calculating the weighted average of adjusted costs and similarity values of similar R&D projects. A case of "Prevention method and system for the diffusion of mobile malicious code" is presented to illustrate the proposed approach.

A Study on the possibility to apply the characteristics of New Urbanism and our country in a new city business seen from the point of view of modernism and post-modernism - Focus on Eunpyung Newtown No. 1 District - (모더니즘과 포스트모더니즘의 관점에서 본 뉴어바니즘의 특성과 우리나라 신도시 사업에 적용가능성에 관한 연구 - 은평 뉴타운 1지구 개발을 중심으로 -)

  • Park, Jong-Hyun;Lee, Jong-Ryul
    • Journal of The Korean Digital Architecture Interior Association
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    • v.12 no.4
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    • pp.107-116
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    • 2012
  • Planning a major change in the domestic residential complex 'complex' in the 'city' to the influx of urban space. So, considering a set of self-environmental complex of openness and connectivity between cities inflow from residential urban housing is formed. Complex-oriented development approach and apartment high-rise and high-density regions of space and social disconnection that causes a uniform methodology that can solve the problem of housing plan, however, a recent New Urbanism New Urbanism has been introduced. And intravenous forms of communal life that occurred in the United States prior to World War II, this value is based on the main form. Design reorganizes This modern lifestyle factors (such as housing, jobs, shopping, leisure space) to go back to the traditional lifestyles while Neotradiotional Planning exercise. New Urbanism in the late 20th century, some literature refers to a postmodern approach adopted in the field of urban planning, the specific case. Actually important feature of post-modernism in the New Urbanism has been expressed. Problem is very confusing, and the principles of New Urbanism, New Urbanism, even those who claim that have different social and design views. Therefore, this study explores the postmodern tendencies of the New Urbanism, and based on this, the possibilities for the development of new towns in Korea mainly affected on the theory of New Urbanism in South Korea in Eunpyeong examine.

Proposal of methodology for AI-based new product development: ambidexterity approach (인공지능 기반 신제품 개발 방법론 제안: 양손잡이(Ambidexterity) 접근)

  • Chung, Doohee
    • Journal of Technology Innovation
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    • v.29 no.4
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    • pp.161-196
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    • 2021
  • This study presents a new methodology for developing AI-based products. It identifies the distinctive attributes of AI innovation that are different from existing methods, and presents a product design process and methodology reflecting these attributes. This study emphasizes that AI product development should be oriented toward an ambidexterity approach. This study proposes a design process and specific development method for AI-based products that including steps such as technology push oriented idea generation with morphological approach, market pull oriented consumer requirements analysis, product design refinement, etc. In order to verify the practical applicability of this methodology, an AI-based car infotainment system development strategy is derived as a case study. 13 innovative ideas were generated by the morphological approach and expert review based on technological possibility, and a total of 6 quality requirements were derived as new product development strategies through the analysis of consumer requirements by combining Kano and TOPSIS. The methodology proposed in this research paper can be usefully utilized for companies to pioneer new markets through AI-based products or to expand the market by upgrading existing products or services.

Analyzing a Class of Investment Decisions in New Ventures : A CBR Approach (벤쳐 투자를 위한 의사결정 클래스 분석 : 사례기반추론 접근방법)

  • Lee, Jae-Kwang;Kim, Jae-Kyeong
    • Proceedings of the Korea Inteligent Information System Society Conference
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    • 1999.10a
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    • pp.355-361
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    • 1999
  • An application of case-based reasoning is proposed to build an influence diagram for identifying successful new ventures. The decision to invest in new ventures in characterized by incomplete information and uncertainty, where some measures of firm performance are quantitative, while some others are substituted by qualitative indicators. Influence diagrams are used as a model for representing investment decision problems based on incomplete and uncertain information from a variety of sources. The building of influence diagrams needs much time and efforts and the resulting model such as a decision model is applicable to only one specific problem. However, some prior knowledge from the experience to build decision model can be utilized to resolve other similar decision problems. The basic idea of case-based reasoning is that humans reuse the problem solving experience to solve a new decision. In this paper, we suggest a case-based reasoning approach to build an influence diagram for the class of investment decision problems. This is composed of a retrieval procedure and an adaptation procedure. The retrieval procedure use two suggested measures, the fitting ratio and the garbage ratio. An adaptation procedure is based on a decision-analytic knowledge and decision participants knowledge. Each step of procedure is explained step by step, and it is applied to the investment decision problem in new ventures.

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Evaluation of the Economic Values and Optimal Deployment Timing of R&D Investment in New and Renewable Energy Using Real Option Approach (실물옵션을 이용한 신재생에너지 R&D의 경제적 가치 및 최적 적용시점 평가)

  • Kim, Kyung-Taek;Lee, Deok-Joo;Park, Sung-Joon
    • Journal of Korean Institute of Industrial Engineers
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    • v.38 no.2
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    • pp.144-156
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    • 2012
  • In recent years, advanced countries in energy sector are emphasizing the importance of the development and deployment of renewable energy to cope with the global environmental crisis such as depletion of fossil energy, climate convention to control emissions of greenhouse gases. In this paper, we evaluate the economic value of the investment in new and renewable energy R&D in Korea and optimal deployment timing of new and renewable energy by using the real option approach. The real option model adopted in this paper assumes that a decision maker has a compound option to abandon, deployment, or continue the R&D. As a result by using empirical data of Korea, it is found that there exists a considerable amount of positive real option value (ROV) in the investment of new and renewable energy R&D while its net present value (NPV) calculated by traditional discounted cash flow (DCF) model shows negative value.

Designing Processes for Ubiquitous-based Sport Business Model (유비쿼터스 기반 스포츠비즈니스모델 설계과정)

  • In, Sang-Woo
    • Journal of Information Technology Services
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    • v.10 no.4
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    • pp.47-65
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    • 2011
  • Business organizations are asked to create new business models utilizing current technological innovations such as ubiquitous computing technology for developing new domains of business to obtain a competitive advantage and achieve a sustainable development. This study was focused on the processes for developing new and practical business models. The purpose of this study was to propose ubiquitous sport business modeling processes from the modeling framework. In particular, this study focused on developing new, pragmatic, and effective sport business models, and this new type of business is defined as 'u-sport.' For design the business model, extensive literature reviews and case studies were conducted for benchmarking the cases and expert group review was conducted for developing u-sport business model framework. The suggested business modeling processes in this study were consisted of four phases; 1) organization strategy level setting phase, 2) business strategy level setting phase, 3) business structure level setting phase, and 4) service level setting phase. The modeling processes were verified to adapt ubiquitous sport business. This designing and modeling process is expected to play a significant role on enhancing the technology-based business environments as the process mainly focuses on the service and consumer oriented approach rather than technology and suppliers oriented approach. In conclusion, establishing sport business models by adapting the service modeling process will deliver an exponential growth and development of future ubiquitous based industry.