• 한국환경농학회지
• /
• 제29권3호
• /
• pp.282-286
• /
• 2010

잘게 부순 유채씨를 토양에 처리하여 토양중의 선충을 친환경적으로 방제하고 오이의 수량을 높이고자 2007년부터 2009년까지 3년간 시험한 결과 토양에 유채종실의 시용량이 많을수록 처리 후 50일에 토양 중 cyanate의 량이 많은 것을 볼 수 있었는데 유채 200 kg/10a을 시용한 토양 중 thiocyanate의 량은 30 mg/kg, 400 kg/10a을 시용한 토양은 40 mg/kg 으로 조사되었다. 토양선충 밀도는 관행의 경우 토양 300 g당 463 마리로 피해유발 수준인 150마리보다 보다 훨씬 높은 밀도로 가시적인 피해가 나타났으나 유채를 처리한 모든 시험구 토양의 경우 토양 300 g당 13~17마리로 뿌리혹 선충의 피해는 나타나지 않았다. 수량은 유채 시 용량이 많을수록 6~15% 증수하는 경향이나 선충 피해 예방과 경제성을 고려할 때 10a당 200 kg를 시용하는 것이 좋을 것으로 사료되었다.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제6권2호
• /
• pp.133-138
• /
• 2003

Five seed kernel namely, Neem (Azadirachta indica A. Juss.), Pongamia (Pongamia glabra L. Pierre), Tamarind (Tamarindus indica L.), Mahua (Madhuca indica Gmel.) and Shikakai (Acacia cancinna De.) were tested against hatching of eggs and larval mortality of Meloidogyne incognita causing root knot disease in mulberry along with Furadan (Carbofuran) and Bionema (a bioformulation developed from Verticillium chlamydosporium) for comparison. Results revealed that highest hush-up of hatching was observed in Neem (77.40%) and Pongamia (75.99%) seed kernel extracts at 100% concentration over the check. Similarly, highest larval mortality was observed in Neem and Pongamia by 76.00% and 74.50%, respectively at 100% concentration after 72 hrs of exposure period. Pot culture studies revealed that pre-application of seed kernel powders (20 days before inoculation of nematode) found to be more effective in controlling the root knot disease than post application. In pre application of seed kernel powders, maximum reduction of root knots was observed in case of Neem seed kernel powder (54.85%) followed by Pongamia (51.9%). Similar trend was also observed in reduction of egg masses/plant and nematode population ／250 cc soil. Rest of the seed kernel extracts was found to be less effective in suppression of hatching, enhancing the larval mortality and controlling the root knot disease. However, application of Furadan and Bionema tested for the comparison were found to be more effective than seed kernel powders. The generated information seems to be useful in developing an ecofriendly integrated approach for the control of root knot nematode disease in mulberry.

• 한국식물병리학회지
• /
• 제14권6호
• /
• pp.710-713
• /
• 1998

Spatial distribution in soil and seasonal population changes of Pasteuria nishizawae first found in Korea from soybean cyst nematode were studied. P. nishizawae infested in 61% of areas in a soybean field; in an average, about 0.3% of 46 cysts and 17.6% of 9 juveniles per 100 g soil were infested with the bacterium. The highest percentages of spore-attached nematodes were found in July (75%) and between soil depth of 11~20 cm. The number of spores per juvenile was positively correlated with the percentage of juveniles with spores (r=0.4203; P＜0.0133), but negatively correlated with the number of juveniles in soil (r=-0.3499; P＜0.042). P. nishizawae completed its life cycle in cyst and produced 1.7$\times$105 spores per cyst.

• The Plant Pathology Journal
• /
• 제32권2호
• /
• pp.123-135
• /
• 2016

U.S. cotton production is suffering from the yield loss caused by the reniform nematode (RN), Rotylenchulus reniformis. Management of this devastating pest is of utmost importance because, no upland cotton cultivar exhibits adequate resistance to RN. Nine populations of RN from distinct regions in Alabama and one population from Mississippi were studied and thirteen morphometric features were measured on 20 male and 20 female nematodes from each population. Highly correlated variables (positive) in female and male RN morphometric parameters were observed for body length (L) and distance of vulva from the lip region (V) (r = 0.7) and tail length (TL) and c' (r = 0.8), respectively. The first and second principal components for the female and male populations showed distinct clustering into three groups. These results show pattern of sub-groups within the RN populations in Alabama. A one-way ANOVA on female and male RN populations showed significant differences ($p{\leq}0.05$) among the variables. Multiple sequence alignment (MSA) of 18S rRNA sequences (421) showed lengths of 653 bp. Sites within the aligned sequences were conserved (53%), parsimony-informative (17%), singletons (28%), and indels (2%), respectively. Neighbor-Joining analysis showed intra and inter-nematodal variations within the populations as clone sequences from different nematodes irrespective of the sex of nematode isolate clustered together. Morphologically, the three groups (I, II and III) could not be distinctly associated with the molecular data from the 18S rRNA sequences. The three groups may be identified as being non-geographically contiguous.

• 한국응용곤충학회지
• /
• 제14권3호
• /
• pp.141-146
• /
• 1975

감교선출(Tylenchulus semipenetrans)에 대한 DBCP(Dibromo chloropropane) $80\%$ 유제의 처리방법 및 약량과 회수를 규명코저 시험하여 아래와 같은 결과를 얻었다. 1. 약량의 증가에 다라, 점주가 관주처리보다 좋은 효과를 보였다. 2. 온주귤원에서는 배수가 나빠서 2회처리로서 좋은 효과를 볼 수 있었다. 3. 점토함량이 낮은 하귤원에는 3.3-4.4l/10a의 1회점주처리가, 점토함량이 높은 온주귤원에는 4.4l/10a씩 2회점주처리가 좋았다.

• 한국식물병리학회지
• /
• 제14권6호
• /
• pp.622-624
• /
• 1998

During the course of study on plant parasitic nematodes associated with medicinal herbs in Korea, high population of different stages of citrus nematode, Tylenchulus semipentrans was found in association with Pyrola japonia Klenze in Imsil, Chunbuk province. The second stage larvae and males were isolated from rhizosphere soil ; immature and mature females were detached from the roots of infested plants of Pyrola japonica. Besides citrus it has been reported to parasitize on roots of Syringa vulgaris, Diospyros lotus, olive, grapevine, and pear in different parts of the world. This is the first record of T. semipenetrans parasitized on a medicinal plant, Pyrola japonica.

• Journal of Microbiology and Biotechnology
• /
• 제28권5호
• /
• pp.831-838
• /
• 2018

Trophic interactions of introduced biocontrol fungi with soil animals can be a key determinant in the fungal proliferation and activity. This study investigated the trophic interaction of an introduced biocontrol fungus with soil nematodes. The biocontrol fungus Trichoderma harzianum ThzID1-M3 and the fungivorous nematode Aphelenchoides sp. (10 per gram of soil) were added to nonsterile soil, and microbial populations were monitored for 40 days. Similar results were obtained when the experiment was duplicated. ThzID1-M3 stimulated the population growth of indigenous nematodes (p < 0.05), regardless of whether Aphelenchoides sp. was added. Without ThzID1-M3, indigenous nematodes did not increase in number and the added Aphelenchoides sp. nematodes almost disappeared by day 10. With ThzID1-M3, population growth of nematodes was rapid between 5 and 10 days after treatment. ThzID1-M3 biomass peaked on day 5, dropped at day 10, and then almost disappeared at day 20, which was not influenced by the addition of nematodes. In contrast, a large quantity of ThzID1-M3 hyphae were present in a heat-treated soil in which nematodes were eliminated. Total fungal biomass in all treatments peaked on day 5 and subsequently decreased. Addition of nematodes increased the total fungal biomass (p < 0.05), but ThzID1-M3 addition did not affect the fungal biomass. Hyphae of total fungi when homogenously distributed did not support the nematode population growth; however, hyphae of the introduced fungus did when densely localized. The results suggest that soil fungivorous nematodes are an important constraint on the hyphal proliferation of fungal agents introduced into natural soils.

• 식물병연구
• /
• 제7권1호
• /
• pp.42-48
• /
• 2001

참외 연작지에서 문제가 되는 뿌리혹선충의 효율적인 방제법을 구명하기 위하여, 땅콩뿌리혹선충이 감염된 성주과채류시험장 온실에서 실험을 실시하였다. 재배적 방제로는 참깨, 파, 옥수수 및 벼를 참외 심기 전에 윤작작물로 재배하였고, 물리적 방제는 건토, 태양열처리 및 객토였으며, 화학적 방제약제로는 forthizate 입제를 사용하였다. 방제효율은 방제 방법간 큰 차이가 있었는데, 벼 윤작, 태양열처리, 객토에서 방제효과가 가장 높아 뿌리혹선충 밀도는 90% 감소하였고 참외 수량은 무처리에 비해 2배 증수되었다. 그러나 이러한 처리구에서 선충밀도가 6월 이후 다시 증가하여 처리효과는 참외 한 작기에만 유효하였다. 다음으로 효과가 높은 것은 옥수수 윤작, 건토, forthizate 처리였다. Forthizate 처리는 초기 수량에서 유의한 증수 효과가 인정되었으나(P=0.05) 후기 수량과 전체 수량에서는 증수 효과가 없었다. 윤작작물로 참깨와 파는 선충 방제 효과가 없었다.

• Journal of Microbiology and Biotechnology
• /
• 제31권6호
• /
• pp.815-822
• /
• 2021

Indigenous fungus-feeding nematodes may adversely affect the growth and activity of introduced biocontrol fungi. Alginate pellets of the biocontrol fungus Trichoderma harzianum ThzID1-M3 and sclerotia of the fungal plant pathogen Sclerotinia sclerotiorum were added to nonsterile soil at a soil water potential of -50 or -1,000 kPa. The biomass of ThzID1-M3, nematode populations, and extent of colonization of sclerotia by ThzID1-M3 were monitored over time. The presence of ThzID1-M3 increased the nematode population under both moisture regimes (p < 0.05), and fungivores comprised 69-75% of the nematode population. By day 5, the biomass of ThzID1-M3b and its colonization of sclerotia increased and were strongly correlated (R² = 0.98), followed by a rapid reduction, under both regimes. At -50 kPa (the wetter of the two environments), fungal biomass and colonization by ThzID1-M3 were less, in the period from 5 to 20 days, while fungivores were more abundant. These results indicate that ThzID1-M3 stimulated the population growth of fungivorous nematodes, which in turn, reduced the biocontrol ability of the fungus to mycoparasitize sclerotia. However, colonization incidence reached 100% by day 5 and remained so for the experimental period under both regimes, although hyphal fragments disappeared by day 20. Our results suggest that indigenous fungivores are an important constraint for the biocontrol activity of introduced fungi, and sclerotia can provide spatial refuge for biocontrol fungi from the feeding activity of fungivorous nematodes.

• 한국환경농학회지
• /
• 제21권1호
• /
• pp.1-6
• /
• 2002

우리나라의 시설참외 주산지인 성주지역의 시설참외 연작 대책으로 객토와 심경에 의한 토양 화학성분의 변화와 뿌리혹선충 억제효과를 조사한 결과는 다음과 같다. 시설참외 연작 대책으로 많은 양의 객토를 함으로 EC는 4.30 dS/m에서 1.80 dS/m 58%, 유효인산은 406 mg/kg에서 182 mg/kg으로 55% 감축되고 유기물 함량이 16 g/kg에서 11 g/kg으로 31% 줄어 퇴비 등 각종 거름 사용량을 증가해야 하며 뿌리혹선충 밀도 억제효과는 1년 차 89%, 3년 차 84%, 5년 차 69%로 되어 근본 대책이 되지 못하면서 영농비 가중 및 환경 파괴의 문제가 수반되었다. 심토반전 효과도 객토와 비슷하여 EC 4.30 dS/m에서 1.98 dS/m 54% 줄고 유효인산 406 mg/kg에서 329 mg/kg으로 19% 줄었으며 뿌리혹선충 밀도 억제 효과는 처리 후 1년 차 71%, 3년 차 67%, 5년 차 42%가 되므로 처리 3년 이후에는 다른 대책이 필요하였다.