• 한국응용곤충학회지
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• 제44권2호
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• pp.145-150
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• 2005

골프장의 문제해충들 중 녹색콩풍뎅이(Popillia quadriguttata) 성충의 방제를 위하여 세 종의 한국산 곤충병원성선충 $\{$Steinernema carpocapsae KCTC 0981BP 계통 (ScK), S. glaseri Dongrae 계통(SgD), Heterorhabditis sp. KCTC 0991BP 계통 (HsK)$\}$을 이용하여 실험을 수행하였다. 3일 후, 녹색콩풍뎅이 성충 한 마리당 900마리의 선충을 처리하였을 때, 치사율은 ScK가 $97.5\%$, HsK가 $90.8\%$, SgD가 $80\%$를 각각 나타냄으로써 이용한 선충 모두가 높은 병원력을 나타내었다. 성충 한 마리 당 900마리의 선충을 처리한 3일 후의 치사율은 ScK는 $v$, HsK는 $90.8\%$, SgD는 $80\%$였다. 선충의 녹색콩풍뎅이 성충 표면부착율과 침입율은 접종 선충의 종과 밀도에 따라 차이가 있었다. 즉, 성충한 마리당 900마리의 선충을 처리하였을 때 녹색콩풍뎅이 성충의 표면에서 검출된 HsK의 비율은 $90.8\%$였으며, SgD는 $90.6\%$, ScK는 $35\%$였다. 녹색콩풍뎅이 성충 내에 침입한 선충의 검출여부에 따른 선충의 침입율은 성충 한 마리 당 900마리의 선충을 처리한 3일 후 ScK는 $97.5\%$, HsK와 SgD는 각각 $80.0\%$를 보였다. 반면, 성충 한 마리 당 90마리의 선충을 처리한 3일 후의 ScK 검출율은 $27.5\%$, SgD는 $72.5\%$였으나 HsK는 발견되지 않았다.

• 한국응용곤충학회지
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• 제51권4호
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• pp.389-395
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• 2012

본 연구는 쌀을 비롯한 저장곡물을 가해하는 어리쌀바구미(Sitophilus zeamais) 성충에 대해서 8종의 식물정유의 훈증독성과 Y-tube를 이용한 후각반응으로 기피효과를 조사하였다. $10{\mu}{\ell}/L$ air의 농도에서 훈증독성시험 결과 pennyroyal과 tea tree 오일에서 각각 97%, 63%의 높은 살충효과를 보였다. 특히, pennyroyal oil($LT_{50}$, hour=3.87)은 tea tree oil($LT_{50}$, hour=4.09)보다 높고 빠른 살충력을 보였다. $10{\mu}{\ell}$/filter paper의 농도에서 후각계를 이용한 기피효과는 Cedarwood(80%), Cinnamon(83.3%), Cypress(81.1%), pennyroyal(97.1%) oil이 높은 기피활성을 보였으며, 이보다 낮은 $1{\mu}{\ell}$/filter paper의 농도에서도 Cinnamon(82.4%), Cypress(80.0%), pennyroyal(86.7%) 오일은 높은 기피활성을 나타냈다.

• International Journal of Industrial Entomology and Biomaterials
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• 제23권2호
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• pp.215-221
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• 2011

To establish an indoor-rearing system for the Large Copper butterfly, $Lycaena$ $dispar$ and the Small Copper butterfly, $Lycaena$ $phlaeas$, the effect of temperature, photoperiod and host plants on larval development was investigated. The larvae of $Lycaena$ $dispar$ fed on $Rumex$ $crispus$, whereas the larvae of $Lycaena$ $phlaeas$ ate both $Rumex$ $crispus$ and $Rumex$ $acetosa$. The duration of the larval period of $Lycaena$ $dispar$ was 13.8 days on $R.$ $crispus$ and that, of $Lycaena$ $phlaeas$ was 15.9 days and 15.2 days on $R.$ $acetosa$ and $R.$ $crispus$ respectively. Laboratory experiments show that the Large Copper larvae are able to feed on other $Rumex$ species without harming their overall survival and can utilize these alternative host plants at least as efficiently as their natural host plant. This result suggests that plant chemistry is not responsible for their lack of utilization of the alternative host plants in the wild. Host plant choice by ovipositing females was measured with the two alternative hosts. $Lycaena$ $dispar$ preferred $R.$ $crispus$ to $R.$ $acetosa$, wheareas $Lycaena$ $phlaeas$ preferred $R.$ $acetosa$ to $R.$ $crispus$. Temperature has been proposed as an important determinant of developmental rate, lifespan and mortality in invertebrates. As temperature increased, length of the developmental period gradually decreased. The developmental period of the Large Copper larvae was 11.0 days and 28.5 days at $30^{\circ}C$ and $17.5^{\circ}C$, respectively.

• 한국패류학회지
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• 제28권1호
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• pp.1-6
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• 2012

2002년 6월부터 2003년 10월까지 충남 태안군 안면읍 내파수도 연안의 수하식 양식장에서 비단가리비 치패 (평균 각고 $32.97{\pm}6.21mm$, 전중량 $5.63{\pm}3.86g$) 를 채롱에 수용하여 밀도에 따른 성장과 생존을 연구하였다. 비단가리비는 주로 한국의 서해안과 중국의 북부 연안에 자연적으로 서식하는 종이다. 조사해역의 표층 수온은 $4.3-25.3^{\circ}C$, 염분은 29.2-32.1 psu, 용존산소는 5.32-7.51 mg/L이었고, pH는 7.84-8.12로 나타났다. 실험을 시작할 때 채롱 당 수용밀도는 20, 30, 40, 50 개체로 하였다. 실험을 시작하여 16개월이 경과한 후의 평균 각고는 64.35-76.23 mm였고, 전중량은 41.53-64.85 g, 생존율은 82-100%로 나타났다. 비단가리비의 성장률은 수용밀도와 역상관 관계를 가지고 있었다. 각고와 전 중량의 성장은 수온이 하강하면서 감소하였다. 비단가리비의 폐사는 대부분 3-4월과 9-10월에 나타났다. 밀도에 따른 생존율은 밀도가 증가함에 따라 감소하였으며 20마리 실험구에서 가장 높게 나타났다.

• Journal of Ginseng Research
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• 제44권2호
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• pp.222-228
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• 2020

Background: 20(S)-ginsenoside-Rg3 (C₄₂H₇₂O₁₃), a natural triterpenoid saponin, is extracted from red ginseng. The increasing use of 20(S)-ginsenoside Rg3 has raised product safety concerns. Methods: In acute toxicity, 20(S)-ginsenoside Rg3 was singly and orally administrated to Kunming mice and Sprague-Dawley (SD) rats at the maximum doses of 1600 mg/kg and 800 mg/kg, respectively. In the 26-week toxicity study, we used repeated oral administration of 20(S)-ginsenoside Rg3 in SD rats over 26 weeks at doses of 0, 20, 60, or 180 mg/kg. Moreover, a 4-week recovery period was scheduled to observe the persistence, delayed occurrence, and reversibility of toxic effects. Results: The result of acute toxicity shows that oral administration of 20(S)-ginsenoside Rg3 to mice and rats did not induce mortality or toxicity up to 1600 and 800 mg/kg, respectively. During a 26-week administration period and a 4-week withdrawal period (recovery period), there were no significant differences in clinical signs, body weight, food consumption, urinalysis parameters, biochemical and hematological values, or histopathological findings. Conclusion: The mean oral lethal dose (LD₅₀) of 20(S)-ginsenoside Rg3, in acute toxicity, is above 1600 mg/kg and 800 mg/kg in mice and rats, respectively. In a repeated-dose 26-week oral toxicity study, the no-observed-adverse-effect level for female and male SD rats was 180 mg/kg.

• 한국동물학회지
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• 제9권1호
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• pp.1-6
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• 1966

Frequently , large masses of the young short necked clam, Tapes japonica , die at their tidal flats in summer and this phenomenon has not been explained clearly. The purpose of the present investigation is to study the thermal condition and the chlorinity level of tidal flats in which the young clam appears to be injured. A study is also mad efor the burrowing organism in the lower layer of the esturay over which the fresh water flow during the low tide. Observation are made at five places of the tidal flat near Ikawazu Fixheries Laboratory of Tokyo University during the ebb and flow tide period of the spring tide. The diurnal and monthly changes of tidal temperatures and chlorinities are measured. Results of the study are ; 1. The surface temperature of the tidal flat increases with the ebb tide, reaches the highest between 12-14PM, and gradually decreases thereafter. The temperatures of tidal flat below 5 and 10 cm increase gradually until the flow tide reaches the surface. 2. At the spring tide in summer , the diurnal change of surface of the tidal flat temperature is very extensive ; it reaches 37-39$^{\circ}C$ in August. At the depths of 5 and 10 cm the temperature remains at 33 $^{\circ}C$ and 31$^{\circ}C$ , respectively. 3. The chlorinity of the tidal flat is higher during May through June and lower July through August, and this seems to be related to the amount of rainfall. 4. The chlorinity of the surface of tidal flat increases slightly during the ebb and flow tide periods. The observed higher chlorinity of surface of the tidal flat was 18.82% Cl. 5. At near the esturay, the fresh water that overflows the tidal flat affects the chlorinity of the surface but no such influence to the depth of the flat. 6. From above observations, it is assumed that the young short necked clam in the tidal flat could be exposed to the severe change of environmental conditions. The high temperature of the tidal flat in summer and the low chlorinity of it at flood period may be considered as the change in environment.

• 한국동물학회지
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• 제9권1호
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• pp.7-15
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• 1966

In a previous paper, the dirunal and monthly changes of the tidal flat temperature and chlorinity were observed. In this paper the resistance of the young short necked clam in various growth stages to the temperature, to the chlorinity and to the exposure were studies. The young clam used were in early (E), medium (M) and late (L) young stages whose shell lengths were 2.0-3.5mm, 9.0-11.0mm. and 14.0-16.0mm., respectively. The results were : 1. At various sea water temperatures , the chlorinity resistance of the young clam was directly proportional to the shell length. 2. When both inadequate sea water temperature and abnormal chlorinity are simultaneously applied, the resistance of these young clams was more markedly reduced than the case of applying either one of these conditions. 3. In clams of M and L, no lethal effect were observed when daily immersion of four to eight hours for a week into the sea water of any concentration of chloriity at 26-34$^{\circ}C$, whereas in E, 37-90% of mortalities were obtained by immersion of eight hours daily into both the fresh water and the sea water of higher chloriniites (more than 23.5$\textperthousand$ Cl) at the same temperature above. 4. The lower critical thermal maximum for lethal to the young clam was 38$^{\circ}C$. With four hours immersion daily at the water temperature of 38$^{\circ}C$, the mortalities of E, M and L to the lower chlorinities (less than 6.7$\textperthousand$Cl) were 100, 70-100 and 27-37% respectively ; to the higher chlorinities (more than 23.5$\textperthousand$Cl) 10-70, 10-37 and 3 % respectively ; to the normal range of chlorinities (13.4-16.8$\textperthousand$Cl) 0-13, 3 and 0 % respectively. 5. No lethal effects were observed in E and M clams immersed continuously for seven days in sea water with chlornities of 7.2 -21.7$\textperthousand$Cl at 18-24$^{\circ}C$, while notable mortalities were observed in E which had been kept at lower (less than 4.8$\textperthousand$ Cl) and higher (more than 24.1$\textperthousand$ Cl) chlorinites. 6. Although the resistance of the young clam to the chlorinity may have to be related closely to the life history of the clam prior subject to the experiment, the adapted chlorinity range was 7.2-19.3$\textperthousand$ Cl and the optimum range was 13.4-16.8$\textperthousand$Cl. 7. Remarkable lethal effects were observed for the E and M clams to the exposure temperature of 38$^{\circ}C$ whereas the L and had no such fatal results.

• 보존과학회지
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• 제28권4호
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• pp.395-401
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• 2012

정유 상태의 세신(Asarum sieboldii) 추출물이 유기질 문화재 보존을 위한 천연 살생물제로써의 적합성 여부를 탐색하기 위하여 목재부후균과 궐련벌레에 대한 항진균활성 및 살충활성을 조사하였다. 목재부후균 4종에 대한 세신 정유추출물의 항진균활성($IC_{50}$)을 측정한 결과 $1.50{\sim}2.84{\mu}l/disc$으로 나타났으며, 이 중 L. lepideus에서 활성이 가장 우수하였다. 궐련벌레 성충에서는 50%(v/w)과 100%(v/w)의 농도로 처리했을 때 약 90% 이상의 살충활성을 보였다. 가스크로마토그래피-질량분석기를 이용하여 정유추출물의 성분을 분석한 결과 총 35종의 화학성분이 검출되었으며, 주성분은 methyleugenol(56.32%), eucarvone(11.53%), safrole(5.79%), ${\delta}$-3-carene(2.09%) 순으로 나타났다. 따라서 세신 정유추출물은 목재부후균과 궐련벌레 등 곤충에 의해 야기되는 생물학적 열화로부터 유기질 문화재를 보존할 수 있는 천연 살생물제로써 활용 가능성을 확인하였다.

• 환경생물
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• 제31권4호
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• pp.353-361
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• 2013

해양에서 이산화탄소 증가에 따른 해양산성화가 넙치치어의 성장에 미치는 영향을 조사하기 위하여 현장 메조코즘 실험을 실시하였다. 동해 남부에 위치한 기장군 장안읍의 사질저질에서 실험군(이산화탄소 주입)과 대조군의 실험장비를 설치하여 43일 동안 총 447개체 (실험군 223개체, 대조군 224개체)를 대상으로 실시하였다. 실험군의 pH는 평균 $7.63{\pm}0.13$, 이산화탄소 ($fCO_2$)농도는 평균 $1660{\pm}540$ ${\mu}atm$로 2100년도에 예상되는 수준으로(IPCC 2007) 유지하였으며, 대조구는 현재 기장군 장안읍 해역의 해양환경으로 유지하였다. 실험기간동안 사망률은 실험군과 대조군 사이에서 큰 차이를 나타내지 않았고 시간이 지남에 따라 감소하는 경향을 나타내었다. 넙치치어의 체장과 체중은 대조군에서 더 큰 증가양상을 나타내어, 그 결과 대조군에서 더 큰 성장률(specific growth rate)을 보였다. 결론적으로 본 연구는 해양에서 이산화탄소 농도의 증가는 넙치 치어의 초기 성장에 영향을 줄 수 있는 가능성을 제시하였다.

• 대한예방한의학회지
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• 제5권1호
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• pp.116-133
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• 2001

Antifibrotic drugs could be delayed or inhibited massive collagen deposition in liver tissue or inhibited collagen synthesis. we investigated antifibrotic effects by several herbs(Schisnadra chinensis, Ganoderma japonicum, Sedum sarmentosum, Alisma canaliculatum, Plantago asiatica) extract with observations of collagen accumulation in liver tissue and collagen synthesis in sera. Rats were used for experimental animal that were devided 3 groups(sham control, BDL/S, BDL/S-ER). Rats were operated for inducing liver fibrosis(cirrhosis) by bile duct obstruction. Several herbs were prepared by water extraction and were applicated p. o. $3ml/day$ during 4 weeks. After observation period, rats were sacrificed and liver tissue and sera were collected. In result, the mortality of rats was 35% in BDL/S group and 20% in BDL/S-ER The color of bile juice in BDL/S-ER was bright yellow and murky yellow in BDL/S group. The significantly lower weight of liver($16.21g{\pm}5.3,\;20.58{\pm}2.4$) and spleen($1.96g{\pm}0.96,\;3.93{\pm}0.21$) were shown in BDL/S-ER than that of BDL/S(p<0.05) group. The value of collagen in liver tissue(25.7%) in BDL/S-ER was observed significantly lower than that of BDL/S group (Tab. 2). AST. ALT, ALP, t-bilirubin, BUN levels were low in BDL/S-ER as compared with those of BDL/S group, but the significance was not proven. The trichrome stained liver tissue in BDL/S-ER group was observed mild bile duct proliferation and fibrosis compared with BDL/S group. In conclusion, natural products inhibited new collagen synthesis and delayed massive collagen deposition in liver tissue, so that they have noticeable antifibrotic effects in experimental liver fibrosis(cirrhosis).