• Journal of Microbiology and Biotechnology
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• 제8권1호
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• pp.42-48
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• 1998

In order to maximize the secretory expression of human serum albumin (HSA) in the yeast Saccharomyces cerevisiae, a series of HSA expression vectors were constructed with a combination of different promoters, 5' untranslated regions (5'UTR), and secretion signal sequences. The expression vector composed of the galactose-inducible promoter GALl0, the natural 5'UTR, and the natural signal sequence of HSA directed the most efficient expression and secretion of HSA among the constructed vectors when introduced into several S. cerevisiae strains. Although the major form of HSA expressed and secreted in the yeast transformants was the mature form of 66 kDa, the truncated form of 45 kDa was also detected both in the cell extract and in the culture supernatant. The level of the intact HSA protein in the culture supernatant reached up to 30 mg/l at 24 h of cultivation in a shake-flask culture but began to decrease afterwards, indicating that the secreted HSA protein was unstable in a prolonged culture of yeast.

• 복식
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• 제54권6호
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• pp.13-23
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• 2004

To date, Italian fashion rans the top even its rise is far behind Paris and New York. The purpose of this study is to analyze and compare the aesthetic characteristics of the italian fashion through two representative designers who contributed to italian fashion. Italy had unique industrial system and craftsmanship from the history and sense of the beauty and the uniqueness from the culture. After the World War II, Italy started to produce elegant sportswears influenced by the progressive manufacturing techniques and practical culture of America. Italian fashion of 'easy elegance' is spearheaded by Vesace and Armani. The aesthetic characteristics of Italian fashion which are shown in the artworks of them are as follows. First, the characteristics of Versace dated from southern sensibility are sensualism, expressionism, historicism. 1) Sensualism: tight silhouette, overexposure, glamorous form, brilliant color, fabric showing femininity, gaudy accessaries 2) Expressionism: brilliant print and color, trimmings and accessaries 3) Historicism: form and motifs that are shown in the historical costume Second, the characteristics of Armani succeeded from northern tailoring are purism, functionalism, and multi-culturism. 1) Purism: clean lines, architectural and geometric forms, natural body line, neutral tones, luxurious fabric 2) Functionalism: functional form, neutral tone, natural fabrics 3) Multi-culturism: design and motif inferred from ethnic costume As such. competition between two different groups made it possible that Italian fashion occupies a special place in the international fashion system.

• Journal of Microbiology
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• 제34권3호
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• pp.241-247
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• 1996

The stability of the genetically engineered microorganisms and their recombinant plasmids released in natural environments has been regarded as one of the molecular ecological topics. In this study, the recombinant plasmids pCU103 in which the pcbCD genes involved in biodegradation of biphenyl and 4-chlorobiphenyl were cloned in pBluescript SK(+) vector, were examined for their structural and functional stability in different waters at 15 $^{\circ}C$ by the methods of electrophoresis, Southern hybridization, quantification with fluorescent dye, and transformation. The recombinant plamids maintained their stabilities for about 30 days in sterilized distilled water (SDW), 15 days in autoclaved creek water (AW), 25 days in filtered and autoclaved non-sterile creek water (FAW), 4 days in Luria-Bertani (LB) broth, and less than one day in filtered non-sterile creek water (FW). The covalently closed circular (CCC) form of the plasmid was decreased and open circular (OC) form was increased as a function of incubation time, and then linear (L) form was produced to be ultimately degraded out. The degradation rates of the plasmid were proportionally correlated to trophic level of the water, and the biological factor such as DNases was found to be one of the most critical factors affecting structural and functional stability of the plasmid in non-sterile natural water.

• Animal Systematics, Evolution and Diversity
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• 제33권2호
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• pp.65-72
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• 2017

This study reports three species in the genus Tubastraea from Korea: Tubastraea coccinea Lesson, 1829; Tubastraea faulkneri Wells, 1982; and Tubastraea micranthus (Ehrenberg, 1834). Tubastraea faulkneri and T. micranthus are newly recorded in Korea. The specimens of three species were collected in the subtidal zones off Jeju-do between 1991 and 2010. The two newly recorded species were described in detail based upon the morphological characters of skeletal structures. The previous records of T. coccinea in Korea were supplemented with additional data in the remarks. These three species have a straight septal arrangement or irregular septal fusion in common as a main character for the genus Tubastraea, but they differ with respect to the growth form, intercorallite distance, exsertness from common coenosteum, and the detailed characters of septal arrangement. Tubastraea faulkneri is similar to T. coccinea in its plocoid growth form, well developed common coenosteum, and corallite size. However, unlike the latter species, the former species is characterized by rare or absent budding adjacent to the corallite edges, wider intercorallite distance, and irregularly developed septal fusion near the columella. In particular, T. micranthus is distinguished by an axial dendroid growth form, and the smallest corallites in this genus.

• Journal of Microbiology and Biotechnology
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• 제33권3호
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• pp.348-355
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• 2023

Epifluorescence microscopy with image analysis was evaluated as a biofilm quantification method (i.e., quantification of surface area colonized by biofilms), in comparison with crystal violet (CV) staining. We performed different experiments to generate multispecies biofilms with natural and artificial bacterial assemblages. First, four species were inoculated daily in 16 different sequences to form biofilms (surface colonization, 0.1%-56.6%). Second, a 9-species assemblage was allowed to form biofilms under 10 acylase treatment episodes (33.8%-55.6%). The two methods comparably measured the quantitative variation in biofilms, exhibiting a strong positive relationship (R² ≥ 0.7). Moreover, the two methods exhibited similar levels of variation coefficients. Finally, six synthetic and two natural consortia were allowed to form biofilms for 14 days, and their temporal dynamics were monitored. The two methods were comparable in quantifying four biofilms colonizing ≥18.7% (R² ≥ 0.64), but not for the other biofilms colonizing ≤ 3.7% (R² ≤ 0.25). In addition, the two methods exhibited comparable coefficients of variation in the four biofilms. Microscopy and CV staining comparably measured the quantitative variation of biofilms, exhibiting a strongly positive relationship, although microscopy cannot appropriately quantify the biofilms below the threshold colonization. Microscopy with image analysis is a promising approach for easily and rapidly estimating absolute quantity of multispecies biofilms.

• 건축역사연구
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• 제5권1호
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• pp.18-38
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• 1996

Dosan Sedans was built in 1560, when Toege I Whang(1500-1571) was 60, as a place in which to study and teach disciples. The building was built to a humble 3-kan system which was a popular form of sodang in the 16th century, There found an enlargement of space by attaching outer walls around three sides which was designed by Toege himself. Minimum in space, moderation in form, conrol of embellishment, hermit scholar's aethetics became basic concept of the architecture of Dosan Sodang. After completion of sodang, Toege wrote many poems about pond, walls, flower bed and natural surroundings like sheer cliff, winding river and even rural daily life of villagers around the building. It could be sud that ,for Toege, architecture was cogniged as a whole complex of building and its surrounding natural, artificial and human circumstances.

• Animal Systematics, Evolution and Diversity
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• 제31권3호
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• pp.146-152
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• 2015

Two scleractinian species are newly recorded in Korea: Leptoseris mycetoseroides Wells, 1954 and Phyllangia hayamaensis (Eguchi, 1968). The two genera Leptoseris Milne Edwards and Haime, 1849 and Phyllangia Milne Edwards and Haime, 1848 are also newly recorded in Korea. The specimens were collected from the subtidal zones of Jeju-do Island, Korea by SCUBA diving from 1991 to 2007. Leptoseris mycetoseroides is characterized by its platelike growth form, intratentacular budding, irregularly developed collines, single styliform columella, and even septa and septocostae. Phyllangia hayamaensis is distinguished by its encrusting and plocoid growth form of corallites basally united with common coenosteum, trabecular columella, and irregular septal arrangements and paliform lobes.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제9권1호
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• pp.53-60
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• 2003

In this study, I investigate the critical evaluation elements at the architectural competition held nationwide in Germany. These evaluation elements, which can be regarded as the important design evaluation criteria, are selected by the design competition committee. Generally, in Germany the design competition committee consist of professors and famous architects who have theoretical and practical abilities with their own office. I classify and analyze the critical evaluation elements of seventy committee members, working on the selected nine prize-winners in Germany from 1997 to 2001. I conclude that the critical evaluation elements, ordered by frequency are : (1)circulation, (2)context, (3)function, (4)landscape & outside space, (5)organization of space, (6)arrangement of building, (7)form, arrangement of building, (7)form, (7)growth & change, (9)elevation design, (10)utilization of natural light. These design evaluation growth & change, (9)elevation design, (10)utilization of natural light. These design evaluation criteria for Hospital Architecture in Germany also can be applied to the evaluation methods of the korean Healthcare facility design.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2006년도 추계학술대회
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• pp.58-61
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• 2006

n-Pentane and n-hexane, previously regarded as non-hydrate formers, are found to form structure H hydrate in mixtures with 2,2-dimethylbutane. Even though they are thought to be too large to fit into the largest cage of the structure H hydrate, powder XRD and NMR measurements show that they form gas hydrates in mixtures with other sH hydrate former. These findings are of fundamental interest and also will impact the composition and location of natural gas hydrates and their potential as global energy resource and climate change materials.

• 로봇학회논문지
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• 제15권1호
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• pp.77-89
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• 2020

This paper proposes a simple and intuitive model-free torque-tracking control for rotary electro-hydraulic actuators. The undesirable natural-velocity-feedback effect is discussed by introducing mechanical impedance into the electro-hydraulic actuation system. The proposed model-free torque control comprises inner- and outer-loop control to achieve two control objectives. Inner-loop control reduces the mechanical impedance passively and optimally. To improve the tracking accuracy, a certain form of proportional-integral-derivative control is applied to the outer loop. The robustness of the proposed closed-loop system against external disturbances is demonstrated by transforming the two-loop control structure into a disturbance observer form. The proposed method is validated on a single joint electro-hydraulic actuator.