• 생명과학회지
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• 제15권5호
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• pp.715-725
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• 2005

식용 및 약용 식물 215 종의 천연물로부터 추출부위를 달리한 메탄올 추출물 309 종을 조제하였으며, 안전성이 우수한 천연물 충치예방 및 치료제 개발을 목표로 309종의 추출물의 S. mutans JC-2에 대한 항균 활성을 평가하였다. 항균활성은 96 well microtiter method에 의한 생육저해능 측정 및 disk paper method에 의한 생육저지환 크기를 측정하여 평가하였으며, 그 결과 가죽나무(가지), 곰딸기(열매), 독활(잎), 상수리나무(잎), 여뀌(전초), 작약(종자), 짚신나물(뿌리) 추출물에서 강한 활성을 확인하였다. 상기 7종 천연물의 최소 생육저지농도(MIC)를 측정하여 항균 활성을 비교한 결과, 여뀌, 상수리나무, 가죽나무 추출물이 $25\∼30{\mu}g/ml $의 농도에서 생육을 완전히 억제하였다 이러한 결과는 항균력이 우수한 천연물로부터 새로운 충치 예방 및 치료제 개발이 가능함을 제시한다

• 한국미생물·생명공학회지
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• 제38권3호
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• pp.289-294
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• 2010

단삼(Salvia miltiorrhiza Bunge)은 우리나라에서 전통적으로 부종, 관절염, 간염 등과 같은 염증질환의 치료에 사용해왔다. 본 연구에서는 단삼 메탄올 추출물을 이용하여 구강미생물에 대한 항균활성을 확인하였다. 그 결과 메탄올추출물은 실험 되어진 모든 구강미생물에서 강한 항균활성을 나타내었다(MICs, 8 to $64\;{\mu}g/mL$; MBCs, 16 to $64\;{\mu}g/mL$). 추가적으로 ampicillin이나 gentamicin과의 병용투여서 최소억제농도(MIC)와 최소살균농도(MBC)가 감소하는 병용효과를 나타내었다. 더불어 메탄올추출물 단독 사용시 보다 항생제와 병용투여시 빠른 사멸효과를 보였다. 결론적으로 단삼 메탄올추출물이 치아우식을 유발하는 많은 세균들에 대한 항균효과가 뛰어남을 확인 할 수 있었으며, 안정성이 높은 천연 치아우식예방제로서의 개발가능성을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제24권9호
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• pp.1232-1237
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• 2014

Lycopene, which is a well-known red carotenoid pigment, has been drawing scientific interest because of its potential biological functions. The current study reports that lycopene acts as a bactericidal agent by inducing reactive oxygen species (ROS)-mediated DNA damage in Escherichia coli. Lycopene treatment elevated the level of ROS-in particular, hydroxyl radicals ($^*OH$)-which can damage DNA in E. coli. Lycopene-induced DNA damage in bacteria was confirmed and we also observed cell filamentation caused by cell division arrest, an indirect marker of the DNA damage repair system, in lycopene-treated E. coli. Increased RecA expression was observed, indicating activation of the DNA repair system (SOS response). To summarize, lycopene exerts its antibacterial effects by inducing $^*OH$-mediated DNA damage that cannot be ameliorated by the SOS response. Lycopene may be a clinically useful adjuvant for current antimicrobial therapies.

• 한국식품저장유통학회지
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• 제8권2호
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• pp.206-212
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• 2001

Water extract of green tea(GTW) and 70% ethanol extract of green tea(GTE) were prepared for the test of antibacterial activity. The sensitivity of Staphylococcus aureus and Salmonella typhimurium to the green tea extracts in various pH of culture broth was tested. Tryptic soy broth(TSB) containing 0∼2%(w/v) of green tea extracts was adjusted to pH 5.0∼7.0 and inoculated with 10$\^$5/∼10$\^$6/ cells/ml of each bacteria. The plate counting method and clear zone test were used to determine inhibitory effect of green tea extracts. Green tea extracts completely inhibited the growth of S. aureus at 0.5% level and bactercidal at 0.5∼1.0% level of GTW and GTE at pH 5.0∼7.0. Green tea extracts were bactercidal to S. typhimurium at 1.5∼2.0% level of GTW and 1.0∼2.0% level of GTE at pH 7.0. Sal. typhimurium was more resistant than S. aureus. in same concentration of green tea extracts at same pH. The resistance of S. aureus and Sal. typhimurium was increased with decreasing pH of culture broth. The morphology of S. aureus cells treated with green tea extracts showed damage of cell wall and cytoplasmic membrane. Severely damaged cells of S. aureus lost electron dense material and cytoplasm. Green tea extracts stimulated autolysis and cell death of S. aureus. This result suggests that green tea extracts can be used as an effective natural antibacterial agent in food.

• 동의생리병리학회지
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• 제19권5호
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• pp.1233-1237
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• 2005

Catechin products in green tea extract was prepared to investigate antibacterial activity on the pathogenic bacteria. Survival of pathogenic bacteria (MASA - methicillin resistant Staphylocouus aureus, E.coli O157 and S. typhimurium Sal-13) in tryptic soy agar(TSA) containing Catechin products powder incubated at various concentration was evaluated. TSA containing $0{\sim}2%(w/v)$ of Catechin products was inoculated approximately $10^4\;CFU/ml$ of pathogenic bacteria and incubated at $37^{\circ}C$ for 24 hours. The plate counting technique and clear zoon test were used to test survival effect of the Catechin products. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was derived from the survival curves of pathogenic bacteria. S. typhimurium Sal-13 was the most sensitive strain to Catechin products. This result suggested that Catechin products can be used as an effective natural antibacterial agent.

• 미생물학회지
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• 제43권3호
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• pp.227-231
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• 2007

구강청정제에 사용되는 많은 항미생물 성분들은 치주조직의 착색 및 작열감 등의 부작용을 가지고 있다. 이러한 취약점을 해결하고, 좀 더 생체친화적인 항미생물 물질을 찾기 위하여,한국식물추출물은행에서 소장하고 있는 2,125종의 식물추출물을 검색하였다. 항미생물 능력을 확인하기 위해, 잘 알려진 치과 병원균(치주질환 3종, 충치 1종)에 대한 성장억제 효과를 측정하였다. 대표적인 치주질환 유발세균인 Porphyromonas gingivalis에 대해서 19중의 식물 추출물이 항생효과를 나타내었으며, 17종이 Prevotella intermedia에 대해, 45종이 Haemophilus actinomycetemcomitans에 대해, 그리고 85종의 추출물이 충치 유발세균인 Streptococcus mutans 에 대해 효과를 나타내었다. 이중에서 총8종의 식물추출물이 실험에 사용한4종의 미생물 중 3종 이상에 대해 항생작용을 나타내었다. 또한 Cudrania tricuspidata, Morus bombycis와 Mallotus japonicas가 실험에 사용된 4종의 치과 병원균 모두에 대해 항생작용을 나타내어 치과용 재료 혹은 양치액에 항미생물 물질로 사용될 가능성을 나타내었다.

• 한국염색가공학회지
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• 제21권1호
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• pp.38-45
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• 2009

First of all, the properties imparted to PET fabrics are resistance to and recovery from creasing or wrinkling when wet or dry; high resistance to stretch in the filament yarns but not in the staple; high abrasion resistance; good texture and appearance; resistance to heat ageing; good chemical resistance and good resistance, behind glass, to sunlight. But, the low moisture regain of PET fabric conduces to static troubles in textile processing. Furthermore, garments made from PET may, during wear, develop electric charges which attract to the fabric particles of soil(dirt, swarf, dust) flying in the air, so that the cuffs of shirts, for example, become soiled quickly and are not easily laundered clean. The sericin constitutes 25$\sim$30％ of silk protein and surrounds the fibroin fiber with sticky layer that supports the formation of a cocoon. The useful biochemical properties of sericin protein are oxidation resistant, antibacterial, UV resistant, hydrophilic property, and good affinity with hydrophobic material. These properties can be used as an improving reagent or a coating agent for natural and synthetic fibers, fabrics, and other intermediate products. The sericin is also applied to cross-link, and can be blended with other materials. In this study, we modified the surface of PET fabric by mixture of sericin finishing agent; sericin, polyuretane binder and 1,2,3,4-butanetetracarboxylic acid (BTCA) cross-link agent. Also, we investigated the finshing effect; moisture regain, stiffness, handle, drape and electrostatic. The moisture regain of PET fabric treated with sericin finishing agent was higher than that of untreated PET fabric. As a result of evaluating influence about handle of PET fabrics treated with sericin finishing agent, it was confirmed that the sericin finishing agent could be use as a linen like finishing agent.

• 한국한의학연구원논문집
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• 제12권1호
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• pp.69-75
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• 2006

치면세균막내의 미생물 중 Streptococcus mutans $N_{AKAI}$는 치면세균막형성에 가장 중요한 역할을 하는 원인균으로 알려져 있으며, Streptococcus mutans $N_{AKAI}$는 치아우식을 일으키는 주요 원인균으로 주목을 받고 있다. Streptococcus mutans $N_{AKAI}$균에 항균효과를 나타내는 화합물 acanthoic acid (AA, $C_{20}H_{30}O_2$)을 Acanthopanax koreanum $N_{AKAI}$의 클로로포롬 추출물에서 각종 컬럼크로마토그래피법으로 추출분리하여 ms, 1H-NMR 그리고 13C-NMR 등을 이용하여 구조를 동정하였다. AA의 Streptococcus mutans $N_{AKAI}$에 대한 최소억제농도(MIC)와 최소살균농도(MBC)를 조사하였으며, 이 때 각각 2와 $4\:{\mu}g/mL$을 나타냈었다. 또한 다른 천연물에서 분리된 화합물인 tanshinone IIA는 8/16\;${\mu}g/mL$, 녹차의 추출물과 eucalyptoi은 $250/500\;{\mu}g/mL$이었다. 그리고 또 다른 치주질환의 원균인 Streptococcus sobrinus $N_{AKAI}$, Streptococcus sanguis $N_{AKAI}$ 및 Streptococcus gordonii $N_{AKAI}$의 최소억제 농도의 범위는 $4{\sim}32\;{\mu}g/mL$이었다. 우리의 연구결과에서 Acanthopanax koreanum $N_{AKAI}$에서 분리한 acanthoic acid 치주질환 원인균들을 억제하는 항균제로의 가능성을 확인하였다.

• 약학회지
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• 제49권2호
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• pp.122-127
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• 2005

Dental caries is one of the most common oral diseases in the world. Glucosyltransferase (Gtase) of Streptococcus mutans (S. mutans) plays an important role in the develo pment of dental caries. For the purpose to develop anti- caries, we examined the effect of metabolites isolated from Streptomyces sp. M-20 on Gtase and the growth of S. mutans. Streptomyces sp. M-20 isolated from Mongolian soil showed 95~96% sequence homology with that of Streptomyces lin- colnensis. The metabolites of Streptomyces sp. M-20 were partially purified by extraction with ethyl acetate, silica gel column chromatography and preparative TLC. Partially purified metabolite, red colored component (MR-20) in ethyl acetate fraction showed potent antibacterial activitiy against S. mutans and inhibitory activity against Gtase purified from S. mutans, while another isolated yellow component (MY-20) showed no activity against S. mutans. The inhibitory activity of MR-20 against Gtase was confirmed by activity staining on SDS-polyacrylamide gel electrophoresis. The concentration of MR-20 for 50% inhibition $(IC_{50})$ against Gtase activity was $60{\mu}g/ml$. These results suggest that MR-20 can be developed for antibacterial agent and anticaries.

• Mycobiology
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• 제51권1호
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• pp.1-15
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• 2023

Wound care has become increasingly important over the years. Various synthetic products for wound care treatment have been reported to cause toxic side effects and therefore natural products are in significant demand as they have minimal side effects. The presence of bioactive compounds in medicinal mushrooms contributes to various biological activities which assist in the early inflammatory phase, keratinocyte proliferation, and its migration enhancement which are pertinent to wound rehabilitation. Lignosus rhinocerus (tiger milk mushroom) can reduce the inflammation phase in wound healing by fighting off bacterial infection and modulating pro-inflammatory cytokines expression in the early stage to avoid prolonged inflammation and tissue damage. The antibacterial, immunomodulating, and anti-inflammatory activities exhibited by most macrofungi play a key role in enhancing wound healing. Several antibacterial and antifungal compounds sourced from traditional botanicals/-products may prevent further complications and reoccurrence of injury to a wounded site. Scientific studies are actively underway to ascertain the potential use of macrofungi as a wound healing agent.