• Molecules and Cells
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• v.27 no.4
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• pp.423-428
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• 2009

Superoxide dismutases (SODs) constitute the first line of cellular defense against oxidative stress in plants. SODs generally occur in three different forms with Cu/Zn, Fe, or Mn as prosthetic metals. We cloned the full-length cDNA of the Thellungiella halophila Cu/Zn-SOD gene ThCSD using degenerate RT-PCR and rapid amplification of cDNA ends (RACE). Sequence analysis indicated that the ThCSD gene (GenBank accession number EF405867) had an open reading frame of 456 bp. The deduced 152-amino acid polypeptide had a predicted molecular weight of 15.1 kDa, an estimated pI of 5.4, and a putative Cu/Zn-binding site. Recombinant ThCSD protein was expressed in Escherichia coli and assayed for SOD enzymatic activity in a native polyacrylamide gel. The SOD activity of ThCSD was inactivated by potassium cyanide and hydrogen peroxide but not by sodium azide, confirming that ThCSD is a Cu/Zn-SOD. Northern blotting demonstrated that ThCSD is expressed in roots, stems, and leaves. ThCSD mRNA levels increased by about 30-fold when plants were treated with sodium chloride (NaCl), abscisic acid (ABA), and indole-acetic acid (IAA) and by about 50-fold when treated with UVB light. These results indicate that ThCSD is involved in physiological pathways activated by a variety of environmental conditions.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.3
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• pp.169-175
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• 1985

This experiment was carried out during 1983-1984 to determine an effective method for the introduction of pasture species on nonarable hill country without cultivation. In this experiment, 1.8 and 2.8kg a.i. $ha^{-1}$ rates of glyphosate and a 0.7kg a.i.$ha^{-1}$ rate of paraquat were applied, and also 30, 45 and 60 days intervals between spraying and sowing were used. Establishment of orchardgrass (Dactylis glomerata) was not affected by the application of herbicides, but that of ladino clover (Trifolium repens) was significantly affected by glyphosate and paraquat. It was found that glyphosate and paraquat have no apparent soil residual activity or pre-emergence effect when sprayed 30 to 60 days before sowing. Dry matter yield of pasture species on the herbicide treatments was significantly higher than that on the unsprayed treatments. The highest yield was obtained from the 1.8kg a.i.$ha^{-1}$ rate of glyphosate 45 days before sowing. Botanical analysis at the final cut showed that the shrubs and native grass of original site were reduced to 5% by glyphosate application, but those on the unsprayed control were still 51%. Crude protein content and in vitro dry matter digestibility of herbage on the herbicide treatments were higher than those on the unsprayed treatments. Results indicated that glyphosate was more effective to control resident shrubs and grass than paraquat, and good establishment and higher yield of surface-sown pasture species can be obtained by spraying 1.8 to 2.8kg a.i.$ha^{-1}$ of glyphosate, 30 to 45 days before sowing.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.22 no.3
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• pp.55-71
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• 2019

The purpose of this study is to propose an evaluation method to assess green environments of streetscapes to improve urban street green spaces in Jeonju City. Through a rapid assessment of urban street green spaces, we suggest an objective basis for expanding street green space as well as for adopting sustainable maintenance and improvement measures. We choose 12 sections of streetscapes (roads and sidewalks) to investigate existing street conditions which have more than four lanes and function as major road axes. Six large roads and six medium roads of Jeonju City center area are investigated as pilot assessment study sites. Site inventory checklists consist of environmental characteristics of streetscape, street tree status, and planting condition evaluation. Environmental characteristics of streetscapes are composed of physical and neighborhood factors. For instance, items for physical factors are types and width of road/sidewalks, paving materials, tree protection materials, and green strip. And surrounding landuse is a neighborhood factor. Assessment items for street tree status are street plant names (tree/shrubs/ground cover), size, and planting intervals. Planting condition evaluation items are tree shape, damage, canopy density, and planting types with existence of adjacent green space. Evaluation results are classified into three levels such as A(maintain or repair), B(greening enhancement), and C(structural improvement). In case of grade A, streetscapes have enough sidewalk width for maintaining green strip and a multi-layered planting(in large road only) with fairly good growing conditions of street trees. For grade B and C, streetscapes have a moderate level of sidewalk width with a single street tree planting. In addition, street tree growing conditions are appeared poor so that green enhancement or maintenance measures are needed. For median, only grade B and C are found as its planting growing foundations are very limited in space. As a result, acquiring enough sidewalk space is essential to enhance ecological quality of urban street green. Especially, it is necessary to have green strip with reasonable widths for plant growing conditions in sidewalks. In addition, we need to consider native species with multi-layer plant compositions while designing street green.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.25 no.1
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• pp.1-10
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• 2022

This study was conducted to investigate the germination characteristics through the temperature change treatment compared to the general room temperature immersion treatment of the native species, the Larix kaempferi, and the Berula platyphyllavar. japonica seeds, and to obtain basic data for the production of healthy seedlings with high productivity and efficiency. As a result, the germination rate of the larch seeds showed a significant difference according to room temperature and temperature immersion treatment, and the average germination days of the birch seeds showed a significant difference according to room temperature immersion and temperature immersion treatment. On the other hand, there was no significant difference in germinal uniformity. The results of the study are as follows. First, the results of the analysis of the fallen leaves were the highest germination rate in the treatment of 1.5 days of hot water and 1.5 days of cold water, the fastest germination rate, the lowest average germination day, and the highest germination uniformity. This is the best result in most items, and the change temperature immersion treatment of hot and cold baths was superior to the conventional room temperature immersion treatment. Second, the results of birch tree showed that the germination rate was the highest in 1.5 days of hot water and 1.5 days of cold water. The average germination day is 1.5 days of cold water 1.5 days of hot water 1.5 days, but the difference between 1.5 days of hot water and 1.5 days of cold water is 0.01 days. The highest germinated uniformity was found in 1.5 days of cold water and 1.5 days of hot water. As a result, in the case of larch and birch, the seed temperature immersion (1.5 days of hot water, 1.5 days of cold water) treatment is superior to the existing three-day immersion treatment in various analysis methods, and it is expected that productivity and efficiency can be improved at a low cost in a short period of time at the seedling production site through seeds.

• The Korean Journal of Physiology and Pharmacology
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• v.27 no.4
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• pp.417-426
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• 2023

The TRPM4 gene encodes a Ca²⁺-activated monovalent cation channel called transient receptor potential melastatin 4 (TRPM4) that is expressed in various tissues. Dysregulation or abnormal expression of TRPM4 has been linked to a range of diseases. We introduced the hemagglutinin (HA) tag into the extracellular S6 loop of TRPM4, resulting in an HA-tagged version called TRPM4-HA. This TRPM4-HA was developed to investigate the purification, localization, and function of TRPM4 in different physiological and pathological conditions. TRPM4-HA was successfully expressed in the intact cell membrane and exhibited similar electrophysiological properties, such as the current-voltage relationship, rapid desensitization, and current size, compared to the wild-type TRPM4. The presence of the TRPM4 inhibitor 9-phenanthrol did not affect these properties. Furthermore, a wound-healing assay showed that TRPM4-HA induced cell proliferation and migration, similar to the native TRPM4. Co-expression of protein tyrosine phosphatase, non-receptor type 6 (PTPN6 or SHP1) with TRPM4-HA led to the translocation of TRPM4-HA to the cytosol. To investigate the interaction between PTPN6 and tyrosine residues of TRPM4 in enhancing channel activity, we generated four mutants in which tyrosine (Y) residues were substituted with phenylalanine (F) at the N-terminus of TRPM4. The YF mutants displayed properties and functions similar to TRPM4-HA, except for the Y256F mutant, which showed resistance to 9-phenanthrol, suggesting that Y256 may be involved in the binding site for 9-phenanthrol. Overall, the creation of HA-tagged TRPM4 provides researchers with a valuable tool to study the role of TRPM4 in different conditions and its potential interactions with other proteins, such as PTPN6.

• Korean Journal of Environment and Ecology
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• v.25 no.4
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• pp.601-613
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• 2011

The purpose of this study is to provide an protected wildlife species and potential habitats in Seoul through the overall present status of the species which live in Seoul. This study was performed in Seoul, the entire area of which is 605.52 $km^2$. We analyzed a total of 57 studies, including academic papers and research reports, for the natural ecology in Seoul. As for the results from the study, it was found that a total 1,907 species from 5 taxa were inhabiting and growing within the area. Among the total, plants of 1,656 species accounted for 86.7%, 197 species of wild birds made up 10.3%, 29 species of amphibians and reptiles consisted of 1.5%, and 27 species of mammalia totaled 1.4%. A total of 440 species except foreign species and species legally preserved by the Ministry of Environment, common species and uncertain species were selected as primary candidates. By taxon, it shows 212 species of plants, 180 species of wild birds, 24 species of mammalia, and 24 species of amphibians and reptiles. For extensive research subjects and research data limited in certain insect family, analysis by literature for discovered insects was excluded. The selection criteria for the second candidate species was based on extinction crisis, rarity, historicity and locality, academic value, habitat specificity, etc. The second candidate species from the primary species were selected through taxon experts' evaluations. As for the results of the selection of the second candidates, a total 77 species were selected through the experts' evaluations. Each species by taxon was 25 species for plants, 21 species for wild birds, 5 for mammalia, 10 for amphibians and reptiles and 16 species for insects. A site investigation for the second candidates was conducted to select the final candidate species. For the target areas, the habitats of the second candidates given by the literature and 46 potential areas in Seoul, which show good natural resistance, were finally selected. From the result of the site investigation, it was found that 14 species of plants, 19 species of wild birds, 2 species of mammalia, 4 species of amphibians and reptiles, and 16 species of insects were actually inhabiting Seoul; thus, the final 55 protected species were selected.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.2
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• pp.93-104
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• 2002

A field survey and experiment was conducted from 1996 to 1998 to develop rational technology for turfgrass vegetation of runway side of Incheon International Airport on the reclaimed tidal land in Young-Jong Island. Backfill of the experimental site was finished on August 1995. The experimental site was 8 ha located in the middle of the construction place for the main parking lot in front of the terminal building construction. The experimental field was drained by main open ditch, and divided three main plots, no subsurface tile drain, subsurface tile drain spacing with 22.5m, and with 45 m, respectively. The 17 sub plots were designed to test the effect of soil covering with red earth loam by 5 cm and 20 cm depth, application of chemical compound fertilizers and livestock manures, dressing of artifical soils and hydrophylic soil conditioners. The tested turfgrasses were three transplanting indigenous turfgrasses, Zoysia koreana, Zoysia sinica and Zoysia japonica, and two hydroseeding mixed exotic turgrasses, cool type I(tall fescue 30%, kentucky blue grass 40%, perenial ryegrass 30%), and cool type II(tall fescue 40%, perenial ryegrass 20%, fine fescue 20%, alkaligrass 20%). The soil backfilled with dredged seasand was sand textured with high salt concentration and low fertility. The soil showed high pH, low organic matter and low available phophate contents. The percolation rate was fast with high hydraulic conductivity. Desalinization was fast after installation of the main open drainage system. No subsurface tile drainage effect was found showing little difference in turfgrass growth. The covering and visual growth of turfgrasses were the best in the 20-cm soil covering with compound fertilizer treatment. The covering and visual growth of turfgrasses were satisfactory in the 5 cm soil covering with compound fertilizer treatment and with livestock manure treatments. The hydrophillic soil conditioner treatments were effective but expensive at present. The coverage and visual quality of turfgrasses were good for Zoysia koreana and Zoysia japonica. The coverages of turfgrasses by the hydroseeding with the mixed exotic turfgrasses were less than transplanting of native turfgrasses. In conclusion, for the runway side vegetation purposes, the subsurface tile drainage might not necessary as main open ditch drainage be sufficient due to fast percolation rate of the backfilled dredged seasand. The 5 cm soil covering with red earth might be sufficient for the runway side, but the 20 cm soil covering might be necessary for the runway side where high density of turfgrass coverage was necessary to protect from the airplance air blow.

• Journal of Korean Society of Forest Science
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• v.98 no.4
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• pp.370-378
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• 2009

This study tries to provide the basic direction to form the arboretum with the distinct features by providing the basic data to help the differentiated strategy for each arboretum. For this purpose, the users' pattern, importance of the indicator by the function, and the stimulation and specialization importance were examined for Daegu Arboretum, Gyeongbuk Arboretum and Gyeongnam Arboretum in Gyeongsang Province. The result says, looking into the functions of arboretum, the collection function showed the highest importance in the preservation of the endangered crisis species; the display function showed the highest in the use as the nature experiencing spaces through the plant exhibition; the research function showed the highest in the study on Plant Systematics; the education function showed the highest in the protection of the native plants; and the recreational function showed the highest in the healthy recreational space. In the plan for the promotion of the arboretum showed the highest in the public education program operation such as the narration from arboretum and education for plant. Therefore, it is considered to need the system setup such as the education program, material development and specialist training in terms of the arboretum. For the specialization plan for arboretum in this study, it seem desirable to concentrate on the research and education related to the natural resources renewal, for Daegu Arboretum; to concentrate on the resort site for the protection and display of the species and the disabled visitors by utilizing the geographical traits in the mountains, for Gyeongbuk Arboretum; to create the specialization plan mainly for the tree species suitable for the warm weather and for the children.

• Journal of Dairy Science and Biotechnology
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• v.27 no.2
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• pp.55-62
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• 2009

Milk is called an almost complete food in terms of nutrition, especially for the younger generations because it contains a number of nutrients required for growth and development. Lactose intolerance is defined as a malabsorption of lactose in the intestine with some typical symptoms of abdominal pains and bloating, and occurred at 75% of global populations, which hampers milk consumption worldwide. Lacks of milk consumption in the underdeveloped countries frequently lead to many nutrients deficiencies, so that diseases including osteoporosis, hypertension, and colon cancer are more prevalent in the recent days. Lactose in foods needs to be hydrolyzed prior to intestinal absorption. The hydrolytic enzyme responsible for splitting lactose into its monomeric forms, glucose and galactose, is called as lactase or $\beta$-galactosidase. The former is primarily used as blood sugar and energy source and the latter used in glycolipid synthesis of brain tissues in infants. Lactose is clinically diagnosed with the breath hydrogen production test as well as intestinal biopsy. Reportedly, symptoms of lactose intolerance are widely prevalent at 25% of Europeans, 50 to 80% of Hispanics, South Indians, Africans, and Jews, almost 100% of Asians and native Americans. For the adults, phenotype of lactase persistence, which is able to hydrolyse lactose, is more common in the northern Europeans, but in the other area lactase non-persistence or adult-type hypolactasia is dominant. Genetic analysis on human lactase gene continued that lactase persistence was closely related to the err site of 1390 single nucleotide polymorphism from the 5'-end. To alleviate severity of lactose intolerance symptoms, some eating patterns including drinking milk a single cup or less, consumption along with other foods, whole milk rather than skimmed milk, and drink with live yogurt cultures, are highly recommended for the lactose maldigesters. Also, delay of gastric emptying is effective to avoid the symptoms from lactose intolerance. Frequency of lactose intolerance with conventional diagnosis is thought overestimated mainly because the subjects are exposed to too much lactose of 50 g rather than a single serving amount. Thus simple and accurate diagnostic method for lactose intolerance need to be established. It is thought that fermented milk products and low- or free lactose milks help improve currently stagnant milk consumption due to lactose intolerance which contributes to major barrier in milk marketing especially in Asian countries.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.4
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• pp.510-517
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• 2015

Miscanthus has been considered as the most promising bioenergy crop for lignocellulosic biomass production. In Korea, M. sacchariflorus and M. sinensis can be found easily in all regions. It is a great advantage to utilize as important species with respect to genetic and cross-breeding programs materials for creation of novel hybrids. For successful breeding programs, it is important to precisely understand the variability of flowering traits among Miscanthus species as breeding parents materials. In this study, flowering traits were observed daily in 960 germplasms of two Miscanthus species (M. sacchariflorus and M. sinensis) for growing seasons over 2 years. The flowering process was divided into three stages. ST (sprouting time) was recorded when first leaf of the plant emerged on soil. FS1 (flowering stage 1) and FS2 (flowering stage 2) were recorded when flag leaf was firstly observed, and 1 cm of panicle was showing on at least one stem, respectively. For 2013 and 2014, the latest germplasms exerted flag leaf, i.e. September 30 (DOY of FS1 164.1) and September 4 (DOY of FS1 141.0) occurred M. sacchariflorus cv. Geodae 1 and M. sacchariflorus cv. Uram collected from Southern Korea (Jeollanam-do), while Miscanthus germplasms collected from northern Korea (Gyeonggi-do) which emerged the earliest flag leaf in July and August, significantly decreased DOY. For DOY from ST to FS2, M. sacchriflorus germplasms ranged from 140 to 190 days, and 110 to 170 days for 2013 and 2014. The highest frequency showed to 160 days for 2013, and 150 days for 2014. In M. sinensis germplasms, the highest frequency showed to 180 days for 2013, and 170 days for 2014. In the results of correlation between the day of years from ST to FS2 for 2013 and 2014, M. sacchriflorus and M. sinensis showed high coefficient of correlation (0.70 and 0.89). It can be supposed that flowering characteristics of Miscanthus are largely affected by the unique phenotypic characteristic of native habitat than environmental factors of the current planted site. This study for flowering traits of Miscanthus may provides an important information in order to expedite the introduction as breeding materials for creation of new hybrid.