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Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti

  • Cho, Hyun ji;Hong, Seong Won;Kim, Hyun-ju;Kwak, Youn-Sig
    • The Plant Pathology Journal
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    • v.32 no.1
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    • pp.53-57
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    • 2016
  • Major diseases in grafted cacti have been reported and Fusarium oxysporum, Bipolaris cactivora, Phytophthora spp. and Collectotrichum spp. are known as causal pathogens. These pathogens can lead to plant death after infection. Therefore, some European countries have quarantined imported cacti that are infected with specific fungal pathogens. Consequently, we developed PCR detection methods to identify four quarantined fungal pathogens and reduce export rejection rates of Korean grafted cacti. The pathogen specific primer sets F.oF-F.oR, B.CF-B.CR, P.nF-P.nR, and P.cF-P.CR were tested for F. oxysporum, B.cactivora, P. nicotinae, and P. cactorum, respectively. The F.oF-F.oR primer set was designed from the Fusarium ITS region; the B.CF-B.CR and P.nF-P.nR primers respectively from Bipolaris and Phytophthora ITS1; and the P.cF-P.CR primer set from the Ypt1protein gene region. The quarantine fungal pathogen primer pairs were amplified to the specific number of base pairs in each of the following fungal pathogens: 210-bp (F. oxysporum), 510-bp (B. cactivora), 313-bp (P. nicotinae), and 447-bp (P. cactorum). The detection limit for the mono- and multiplex PCR primer sets was 0.1 ng of template DNA under in vitro conditions. Therefore, each primer set successfully diagnosed contamination of quarantine pathogens in export grafted cacti. Consequently, our methodology is a viable tool to screen contamination of the fungal pathogen in exported grafted cacti.

Screen of Green Manure Crops for Cultivation on Agricultural Land with Spring Season in the Central Regions of Korea

  • Cho, Hyeon-Suk;Seo, Myung-Chul;Kim, Jun-Hwan;Sang, Wan-gyu;Shin, Pyeong;Lee, Geon Hwi
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.6
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    • pp.689-696
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    • 2015
  • The green manure crops are returned to soil when the plants are still green. The green manure crops play a role in reducing chemical fertilizers, improvement of soil fertility, reduction of soil erosion, weed control, and landscapes effect. Typical green manure crops are barley green manure, hairy vetch, chines milk vetch and clovers. We sowed 49 species of green manure crops in upland soil to increase the usage of green manure crops. We searched the effectiveness of green manure yield, C/N ratio, soil mulching, and landscape effect. The green manure crops that have $5tonha^{-1}$ or more of green manure yield (fresh weight) were 6 gramineous crops, 4 leguminous crops, and 3 others. Green manures yield in a short growth period was higher in gramineous crops than leguminous crops. Among the green manure crops, 3 gramineous crops, 12 leguminous crops, and 3 others were under 25 in C/N ratio. Seven gramineous crops, 8 leguminous crops, and 5 others were over 50% of soil covering rate. Green manure crops that have beautiful flowers were in total 16 kinds, such as 4 gramineous crops, 6 leguminous crops, and 6 others. In result, based on green manure yield and C/N ratio, excellent species in the green manure crops were 4 species (triticale, 2 kinds of hairy vetch, and lupin). Green manure crops that were effective at soil mulching were 20 species, like hairy vetch, oats, sorghum, phacelia, buckwheat, etc. Buckwheat, crotalaria, mustard plant, phacelia, and etc. could be available for landscape crops.

Clinical and radiographic features of facial cosmetic materials: A systematic review

  • Alsufyani, Noura;Aldosary, Reem;Alrasheed, Rasha;Alsufyani, Mohammed
    • Imaging Science in Dentistry
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    • v.52 no.2
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    • pp.155-164
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    • 2022
  • Purpose: The aim of this study was to systematically screen the literature for studies reporting cosmetic material in the oral and maxillofacial complex to shed light on the types of cosmetic materials, their radiographic appearance, and possible complications. Materials and Methods: Five electronic databases were reviewed for eligible studies. The general search terms were "cosmetic," "filler," "face," and "radiograph." Demographics, material types, clinical and radiographic presentation, and complications were recorded. Results: Thirty-one studies with 53 cases met the inclusion criteria. The mean age was 52.6±15.4 years with a 4 : 3 female-to-male ratio. The most common material was calcium hydroxyapatite (CaHa) (n=14, 26.4%), found incidentally. The materials were generally located within the upper cheek and zygoma (n=35, 66.0%), radiographically well-defined (n=44, 83%), and had no effects on the surrounding structures (n=27, 50.9%). The internal structure was radiopaque (calcification, hyperdensity) for gold wires, CaHa, bone implants, and secondary calcification or ossification. Outdated cosmetic materials or non-conservative techniques were infiltrative, had effects on the surrounding structures, and presented with clinical signs, symptoms, or complications. Conclusion: Conventional radiography, cone-beam computed tomography, and multi-detector computed tomography are useful to differentiate several cosmetic materials. Their magnetic resonance imaging appearance was highly variable. The infrequent inclusion of cosmetic materials in the differential diagnosis implies that medical and dental specialists may be unfamiliar with the radiographic appearance of these materials in the face.

A Study of the Optimization of the MOF-5 Synthesis Process using Design of Experiments (실험계획법을 이용한 MOF-5 합성공정 최적화 연구)

  • Lee, Min Hyung;Lee, Sangmin;Yoo, Kye Sang
    • Applied Chemistry for Engineering
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    • v.33 no.4
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    • pp.402-407
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    • 2022
  • Statistical design of experiments was used to optimize the MOF-5 synthesis process. A mixture design was employed to optimize precursor concentration. The optimal composition of three chemical materials, terephthalic acid, zinc acetate dihydrate, and N,N-dimethylformamide for MOF-5 synthesis was determined by extreme vertices design methods as follows; 1 mol : 2.7 mol : 40 mol. A multilevel factorial design was selected to screen the significance of synthesis reaction conditions such as temperature, time, and stirring speed. Statistical analysis results suggested excluding stirring speed from further investigation. Using a central composition design, the synthesis time and temperature were optimized. The quadratic model equation was derived from 13 synthesis experiments. The model predicted that MOF-5 synthesized at 119 ℃ for 10.4 h had the highest crystallinity.

Rapid Screen for Bacteria Solubilzing Insoluble Phoshpate on Agar Plate

  • Son, Hong-Joo;Kang, Sung-Il;Kim, Yong-Gyun;Kim, Hee-Goo;Lee, Sang-Joon
    • Journal of Life Science
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    • v.10 no.1
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    • pp.64-65
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    • 2000
  • Insoluble phosphate-solubilizing bacteria are routinely screened by a plate assay method using Pikovskaya agar and a modified Pikovskaya medium. A modified Pikovskaya medium to improve the clarity of the yellow-colored halo has not necessarity improved the plate assay. Colonies of phosphate-solubilizing bacteria tested could be redily selected after 48 h of incubation by green-colored colony formation on plate in which bromcresol green(BCG) was included. Among them, two bacterial strains did not produce distinct yellow halos after 48 h of incubation. We suggest that the green colony formation on plate medium containing BCG is advantageous ofr rapid isolating phosphate-solubilizing bacteria directly from soil.

Service Technology Prospect for Next Generation Smart TV (차세대 스마트TV 서비스 기술 전망)

  • Hong, Jin-Woo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2012.05a
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    • pp.368-371
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    • 2012
  • Smart TV is approaching as a new media service to integrate the broadcasting and the internet according to the technology evaluation of convergence to broadcasting, telecommunication, and computing. Therefore, This paper looks out the next generation smart TV(Smart TV 2.0) technology based on CPNT which supports those services such as the broadcasting, telecommunication, a convergence of broadcasting & communication, and computer service through multi-screen.

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A Forward Genetic Approach for Analyzing the Mechanism of Resistance to the Anti-Cancer Drug, 5-Fluorouracil, Using Caenorhabditis elegans

  • Kim, Seongseop;Shim, Jaegal
    • Molecules and Cells
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    • v.25 no.1
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    • pp.119-123
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    • 2008
  • Pyrimidine antagonists including 5-Fluorouracil (5-FU) have been used in chemotherapy for cancer patients for over 40 years. 5-FU, especially, is a mainstay treatment for colorectal cancer. It is a pro-drug that is converted to the active drug via the nucleic acid biosynthetic pathway. The metabolites of 5-FU inhibit normal RNA and DNA function, and induce apoptosis of cancer cells. One of the major obstacles to successful chemotherapy is the resistance of cancer cells to anti-cancer drugs. Therefore, it is important to elucidate resistance mechanisms to improve the efficacy of chemotherapy. We have used C. elegans as a model system to investigate the mechanism of resistance to 5-FU, which induces germ cell death and inhibits larval development in C. elegans. We screened 5-FU resistant mutants no longer arrested as larvae by 5-FU. We obtained 18 mutants out of 72,000 F1 individuals screened, and mapped them into three complementation groups. We propose that C. elegans could be a useful model system for studying mechanisms of resistance to anti-cancer drugs.

Micro Scale Mechanical property of Polymeric materials for FPD(Flat Panel display) (FPD에 사용되는 고분자 재료의 기계적 물성특성 연구)

  • Lee N.K.;Lee H.J.;Lee H.W.;Chong E.G.;Choi D.S.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.220-224
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    • 2005
  • The technology trend of Flat Panel Display (FPD) equipments have been demanded that there are compact and multi-function. Therefore, nano/micro scale patterned on polymeric materials of Back Light Unit (BLU) in Liquid Crystal Display (LCD) that has been investigated. This paper describes a series of Horizontal Type Micro Tensile Tester that were carried out to investigate the load strain distance performance of typical polymeric material sheets. The polymeric materials film that micro size shaped specimens for tensile test are used by Cold-Isostatic-Press (CIP). Test equipment is Horizontal type Micro Tensile Tester that is presented to measure the micro scale mechanical property of thin film for FPD. This paper presents which easy testing tools measure for micro patterned on polyethylene (PET) specimens.

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Monoterpenoid of Korean Valerian Roots (국산 길초근의 Monoterpenoid)

  • Ryu, Kyung-Soo
    • Korean Journal of Pharmacognosy
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    • v.5 no.1
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    • pp.1-6
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    • 1974
  • Essential oils were separated by steam distillation from the roots of four species of the genus Valeriana and subjected to gas chromatography to screen monoterpenic hydrocarbons and related components. The amounts of the common components were contained in the following order: camphene, ${\alpha}-pinene$, bornyl-, myrtenyl isovalerate, myrtenylacetate and ${\beta}-pinene$, and small amounts of terpineol and terpinylacetate were also contained. Of the four species, the root of Valeriana fauriei $B_{RIQUET}$ var. dasycarpa $H_{ARA}$ contained the largest amounts of these components and the root of V. dageletiana $N_{AKAI}$ ex $M_{AEKAWA}$ the least. Since the root of V. dageletiana contained for less amounts of pinene, camphene and bornylacetate and none of dipentene and terpineol, this plant appears to be distinguished from the other three species.

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CPNT Based Next Generation Smart TV Service Technology (CPNT 기반 차세대 스마트TV 서비스 기술)

  • Hong, Jin-Woo
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.16 no.6
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    • pp.1209-1216
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    • 2012
  • Smart TV is approaching as a new media service to integrate the broadcasting and the internet according to the technology evaluation of convergence to broadcasting, telecommunication, and computing. Therefore, This paper proposes the next generation smart TV(Smart TV 2.0) technology based on CPNT which supports those services such as the broadcasting, telecommunication, a convergence of broadcasting & communication, and computer service through multi-screen. Also, this paper describes the concept and details of the related technologies.