Journal of Life Science

Korean Society of Life Science (한국생명과학회)
권호 표지

Rapid Screen for Bacteria Solubilzing Insoluble Phoshpate on Agar Plate

  • Son, Hong-Joo (Corresponding author, Department of Biotechnoloty, Miryang National University, Miryang 627-702) ;
  • Kang, Sung-Il (Department of Biotechnology, Miryang National University, Miryang 627-702) ;
  • Kim, Yong-Gyun (Department of Biotechnology, Miryang National University, Miryang 627-702) ;
  • Kim, Hee-Goo (Department of Mcirobiology, Pusan National University, Pusan 609-735) ;
  • Lee, Sang-Joon (epartment of Mcirobiology, Pusan National University, Pusan 609-735)
  • Published : 2000.04.01

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Abstract

Insoluble phosphate-solubilizing bacteria are routinely screened by a plate assay method using Pikovskaya agar and a modified Pikovskaya medium. A modified Pikovskaya medium to improve the clarity of the yellow-colored halo has not necessarity improved the plate assay. Colonies of phosphate-solubilizing bacteria tested could be redily selected after 48 h of incubation by green-colored colony formation on plate in which bromcresol green(BCG) was included. Among them, two bacterial strains did not produce distinct yellow halos after 48 h of incubation. We suggest that the green colony formation on plate medium containing BCG is advantageous ofr rapid isolating phosphate-solubilizing bacteria directly from soil.

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