• 한국약용작물학회지
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• 제15권5호
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• pp.329-334
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• 2007

The potential antioxidant and anticancer activities of Hexane, EtOAc (Ethyl acetate), BuOH (n-Buthanol) and water fractions from methanolic (MeOH) extract of Picrasma quassioides (D. Don) Benn. were evaluated in vitro. Tested fractions showed strong antioxidant activity, especially EtOAc fraction had the highest activity ($IC_{50}\;=\;114.01\;{\mu}g/mL$), containing high total phenolics and total flavonoids contents, showed $67.59\;Tan\;{\mu}g/mg$ and $64.95\;Que\;{\mu}g/mg$ respectively. Anticancer activity of these fractions was tested by MTT assay on HT-29 (the human colon carcinoma cells) cell line. BuOH fraction not only showed very high anticancer activity, but also had no cytotoxic effect on 293 (the human normal kidney cells) cell line. Considering these results, we used BuOH fraction of MeOH crude extract from P. quassioides (D.Don) Benn. to do assessment of apoptosis by flow cytometry and the mRNA expression levels of widely established apoptotic-related genes on HT-29 cell line. All the experiments showed that BuOH fraction can induce apoptosis on HT-29 cell line strongly. Taken together, methanolic extract of P. quassioides has potential for antioxidant and anticancer activities products.

• Ocean and Polar Research
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• 제36권3호
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• pp.247-254
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• 2014

In our continuing study on the antioxidant activity of Polysiphonia stricta, its crude extract was fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions according to solvent polarity. The solvent fractions were evaluated for their potential to inhibit lipid peroxidation and reactive oxygen species (ROS) production in HT 1080 cells. The n-BuOH fraction most strongly inhibited both lipid peroxidation and ROS production in HT 1080 cells. The n-BuOH fraction was further separated by repeated silica gel column chromatography and RP-HPLC to give methyl aconitates (2 and 3). The chemical structure of isolated compounds was determinated by NMR spectral analysis.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.191.1-191.1
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• 2003

The anti-oxidant activities of Acanthopanax senticosus stems were investigated. The n-BuOH fraction of A. senticosus stems exhibited a significant decrease in serum transaminase activities elevated by hepatic damage induced by CCl$_4$-intoxication in rats. The n-BuOH fraction inhibited the sGPT activities by 65.79%. The n-BuOH fraction showed the increase in the anti-oxidant enzymes such as hepatic cytoso1ic superoxide dismutase, catalase and glutathione peroxidase activities by 30.31, 19.82 and 155%, respectively, in CCl$_4$-intoxicated rats. These results suggest that the stems of A. senticosus possess not only the hepatoprotective, but also the anti-oxidant activities in rats.

• Ocean and Polar Research
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• 제38권3호
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• pp.185-193
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• 2016

본 연구에서는 북극 육상식물인 R. hyperboreus를 유기용매로 추출하여 얻어진 추출물에 대하여 세포내 ROS와 peroxynitrite 소거활성을 측정하였다. 추출물의 유의적인 소거활성을 확인한 후에 구성성분의 극성에 따른 소거효과의 변화를 보기 위하여 용매분획을 실시하여 n-hexane, 85% aq.MeOH, n-BuOH, water 분획물을 얻었다. 각 용매분획의 폴리페놀 함량을 측정하였으며 그 결과 n-hexane, water, 85% aq.MeOH, n-BuOH 분획의 순서대로 폴리페놀 함량이 증가하였다. 각 용매분획에 대한 항산화 활성을 검색한 결과 n-hexane 분획의 경우에는 세포생존율에 기인한 어느 정도의 활성변화는 있었으나 대체적으로 소거 활성은 폴리페놀 함량에 비례하였다. 폴리페놀 함량이 가장 높은 n-BuOH 분획물이 가장 좋은 항산화활성을 나타내었으며 그 다음 번째로 폴리페놀 함량이 높은 85% aq.MeOH 분획물이 두 번째로 좋은 항산화활성을 나타내었다.

• Advances in Traditional Medicine
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• 제7권3호
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• pp.217-228
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• 2007

Agrimonia pilosa Ledeb. has long been used for a useful natural agent ameliorating inflammation related symptoms in the folk medicine recipe. This study was performed to investigate effects of Agrimonia pilosa Ledeb.(AP) on the expression of inflammation related genes such as the inducible nitric oxide synthase (iNOS) in macrophage cell line, RAW 264.7 cells. The AP (whole plants) was extracted with 80% ethanol and sequentially partitioned with solvents in order to increase polarity. Among the various solvent extracts of AP, the n-butanol (BuOH) fraction showed the most powerful inhibitory ability against nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 cells without affecting cell viability. Reverse transcriptase-polymerase chain reaction and Western blot analysis revealed that the BuOH fraction provided a primary inhibitor of the iNOS protein and mRNA expression in LPS-induced RAW 264.7 cells. The DPPH and OH radical scavenging activities of the several fractions of 80% ethanol extracts of AP significantly increased by EtOAC and BuOH fractions. Thus, the present study suggests that the response of a component of the BuOH fraction to NO generation via iNOS expression provide an important clue to elucidate anti-inflammatory mechanism of AP.

• Natural Product Sciences
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• 제4권2호
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• pp.100-104
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• 1998

The antimutagenic activity of a methanol extract of roasted Cassia tora seed against aflatoxin $B_1(AFB_1)$ was demonstrated with the Salmonella typhimurium assay. The numbers of revertants per plate decreased significantly when this extract was added to the assay system using Salmonella typhimurium TA100 and or TA98. The MeOH extract was then sequentially partitioned with $CH_2Cl_2$, EtOAc, n-BuOH, and $H_2O$. The $CH_2Cl_2$ and n-BuOH fractions possessed antimutagenic activity, but the EtOAc and $H_2O$ fractions were inactive. Both the MeOH extract and its fractions were capable of inhibiting the indirect-acting mutagen $AFB_1$, suggesting that these fractions may prevent the metabolic activation of $AFB_1$, or scavenge the electrophilic intermediate capable of inducing mutations. Column chromatography using silica gel yielded pure alaternin from the $CH_2Cl_2$ a fraction, and adenosine and isorubrofusarin gentiobioside from the n-BuOH fraction. Alaternin and isorubrofusarin gentiobioside demonstrated significant antimutagenic activities.

• 생약학회지
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• 제45권1호
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• pp.35-40
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• 2014

In this study, in order to search for new functional materials from natural products, was carried out the study of antioxidant active ingredients in persimmon calyx(calyx of Diospyros kaki Thunberg). I have experimented with the effect of antioxidant activity of five different extract(MeOH, n-hexane, EtOAc, n-BuOH and $H_2O$ extract) obtained from persimmon calyx. As a result, the butanol extract, that is the main component fraction of antioxidant activity was found. Three compounds were isolated by silica gel column chromatography from the n-BuOH extract of persimmon calyx. Their structures of compound 1, 2 and 3 isolated from n-BuOH extract of persimmon calyx were identified as quercetin, (+)-catechin and gallic acid by using the TLC, $^1H$-NMR and $^{13}C$-NMR.

• Journal of Nutrition and Health
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• 제35권3호
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• pp.291-295
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• 2002

SD계 흰쥐를 사용하여 평균적으로 하루에 솔잎 추출물의 BuOH 획분을 25, 50, 100mg/kg BW가 섭취하도록 사료에 첨가하여 45일 동안 투여하였다. BuOH-25 투여그룹을 제외한 BuOH-5- 및 BuOH-100 투여그룹의 mitochondria 및 microsomes은 대조그룹 대비 11.6%, 20.1% 및 10.5%, 13.5%의 매우 유의적인 간장 내 콜레스테롤 침착 억제효과가 인정되었다. BuOH-25, BuOH-50, BuOH-100 투여그룹의 mitochondria에서는 대조그룹 대비 2.9%, 13.3%, 18.5%의 .OH 라디칼 억제효과로서 BuOH-5- 및 Bu-OH-100 투여그룹에서 높은 유의성이 인정되었으며, microsome에서는 대조그룹 대비 15.7%, 20.0%, 20.6%의 매우 효과적인 .OH 라디칼의 생성 억제효과가 인정되었다. 또한 BuOH-25, BuOH-50, BuOH-100 투여그룹의 간장 cytosol 획분에서는 대조그룹 대비 5.2%, 8.0%, 11.1%의 $O_2$라디칼의 생성 억제효과로서 BuOH-50 및 BuOH-100 투여 그룹에서 유의성이 인정되었다. 간장 mitochondria 획분에서 BuOH-25, BuOH-50, BuOH-100 투여그룹의 Cu/Zn-SOD 활성은 대조그룹 대비 각각 4.6%, 10.3%, 15.9%의 Cu/Zn-SOD 활성 증가효과로서 BuOH-50 및 BuOH-100 투여그룹에서 유의성이 인정되었지만, Mn-SOD 활성은 세가지 BuOH 투여그룹의 유의성은 나타나지 않았다. BuOH-25, BuOH-50, BuOH-100 투여그룹의 간장 cytosol 획분에서는 대조그룹 대비 각각 9.0%, 19.4%, 25.6%의 매우 유의적인 GPx 활성 증가효과가 인정되었다. 따라서 솔잎의 BuOH 획분은 조직의 콜레스테롤의 침착을 효과적으로 억제효과할 뿐만 아니라 활성산소의 생성을 유의적으로 억제하고 제거 효소의 활성을 증가시킴으로써 노화과정을 효과적으로 억제할 수 있을 것으로 기대된다.

• 한국수산과학회지
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• 제43권5호
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• pp.437-444
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• 2010

Extracts of the marine microalgae Nannochloropsis oculata were obtained using 80% methanol (MeOH) and water. The 80% MeOH extract was further fractionated with n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol (n-BuOH), and water to isolate the active fraction. Seven samples were prepared and their angiotensin converting enzyme (ACE), $\alpha$-glucosidase, and cancer cell growth inhibitory activities in vitro were determined. The most profound ACE inhibitory activity was observed in the chloroform fraction, while the others had moderate effects. By contrast, greater $\alpha$-glucosidase inhibitory activity was found in the EtOAc fraction, n-hexane fraction, and water extract of N. oculata. The antiproliferative effects of the extracts and fractions against HL-60, U937, CT-26, and SK-Hep1 cancer cells were also determined. The n-BuOH fraction had the strongest antiproliferative effects on CT-26 cells in a time-dependant manner (P<0.05). These results suggest that the extracts and fractions from N. oculata could be used as a potential functional food or as pharmaceutical ingredients.

• 원예과학기술지
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• 제29권1호
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• pp.68-73
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• 2011

본 연구는 차로써 이용이 증대되고 있는 구절초 꽃의 항산화 능력과 이와 관련된 유효한 성분을 탐색하고자 수행되었다. 구절초 꽃을 MeOH로 추출한 수율은 16%였고, MeOH 추출물을 다시 용매별로 분획한 결과 잔류 water > n-hexane > EtOAc > n-BuOH > $CHCl_3$ 분획물 순으로 추출수율을 보였다. 총 polyphenol 함량은 EtOAc분획물이 $195mg{\cdot}g^{-1}$으로 가장 높았고 그 다음으로 MeOH 추출물, $CHCl_3$, 잔류 water, n-BuOH, n-hexane 분획물 순으로 높게 나왔다. Flavonoid 함량 역시 EtOAc 분획물이 $143mg{\cdot}g^{-1}$으로 가장 높게 나타났으며 그 다음으로는 n-BuOH, n-hexane, 메탄올, 잔류 water, $CHCl_3$ 분획물 순으로 높았다. 구절초의 전자공여능과 총 항산화력 측정에서는 추출물의 농도가 높아질수록 활성이 증가하였으며 EtOAc 분획물이 $100{\mu}g{\cdot}mL^{-1}$에서 81.56%, 68.12%의 가장 높은 전자공여능 및 총 항산화력을 나타내었다. 구절초 MeOH 추출물과 EtOAc분획물을 HPLC로 분석한 결과 flavonoid 유효성분으로 quercetin과 luteolin이 확인되었으며 EtOAc 분획물 경우 각각의 함량이 $20.02mg{\cdot}g^{-1}$, $1.65mg{\cdot}g^{-1}$이었다. 이상의 결과를 종합해볼 때, 구절초 꽃의 항산화력은 MeOH 추출물의 EtOAc 분획에 포함된 quercetin에 주로 기인하는 것으로 판단되었다.