• Parasites, Hosts and Diseases
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• 제60권1호
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• pp.25-34
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• 2022

Alveolar echinococcosis (AE) caused by infection with E. multilocularis metacestode, represents one of the most fatal helminthic diseases. AE is principally manifested with infiltrative, proliferating hepatic mass, resembling primary hepatocellular carcinoma. Sometimes metastatic lesions are found in nearby or remote tissue. AE diagnosis largely depends on imaging studies, but atypical findings of imaging features frequently require differential diagnosis from other hepatic lesions. Serological tests may provide further evidence, while obtaining reliable AE materials is not easy. In this study, alternative antigens, specific to AE were identified by analyzing E. granulosus protoscolex proteins. An immunoblot analysis of E. granulosus protoscolex showed that a group of low-molecular-weight proteins in the range from 14 kDa to 16 kDa exhibited a sensitive and specific immune response to AE patient sera. Partial purification and proteomic analysis indicated that this protein group contained myosin, tubulin polymerization promoting protein, fatty-acid binding protein, uncharacterized DM9, heat shock protein 90 cochaperone tebp P-23, and antigen S. When the serological applicability of recombinant forms of these proteins was assessed using enzyme-linked immunosorbent assay, DM9 protein (rEgDM9) showed 90.1% sensitivity (73/81 sera tested) and 94.5% specificity (172/181 sera tested), respectively. rEgDM9 showed weak cross-reactions with patient sera from the transitional and chronic stages of cystic echinococcosis (3 to 5 stages). rEgDM9 would serve as a useful alternative antigen for serodiagnosis of both early- and advanced-stage AE cases.

• 한국발생생물학회지:발생과생식
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• 제4권1호
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• pp.67-77
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• 2000

심해에서 서식하며 특이한 조직형태를 갖고 있는 꼼치조직에서 cDNA를 제작하여 클로닝을 통해서, 꼼치에서 특이하게 발현하는 유전인자를 검색하였다. 그 결과 62례의 클론이 알려진 유전자에 동질성이 있었는데 이들은 thioesterase가 9례, myosin이 8례, creatine kinase가 7례, skeletal alpha-actin이 6례, parvalbumin b와 ribosomal protein이 각각 5례, type I collagen과 muscle troponin이 각각 3례, dopamine receptor, histatin, 그리고 heat shock protein이 각각 2례, cystatin, lectin, statherin, secretory carrier membrane protein, keratin type I, desmin, chloroplast, muscle tropomyosin, reticulum calcium ATPase, ribonucleoprotein이 각각 1례로 나타났다. 나머지 클론은 동질성이 낮거나 비반복성으로 나타났으며, 이 중 in situ hybridization으로 조직에서 특이하게 발현되는 5종류의 클론을 선택하여 분석하였으며, C 말단 단백질 구조와 특색(motif)을 분석하였다. 5종류의 클론에서 C9O-77은 약 5000bp 크기로 상피조직, 점액조직, 섬유조직 그리고 교원질 조직에서 강한 양성반응을 보이는 세포의 기질단백질로 예상되었다. C9O-116은 약 1500bp 크기로 섬유조직, 상피조직 그리고 점액조직에서 약한 양성반응을 보였고, 근육 다발 주변 부위 세포에서 매우 강한 양성반응을 보이는 막투과성 단백질로 예상되었다. C9O-130은 약 1200bp 크기로 상피조직, 근육조직 그리고 점액조직에서 양성반응을 나타냈으며, 특히 상피조직에서 강한 양성반응을 나타내는 세포내 단백질로 예상되었다. C9O-161은 약 2000bp 크기로 상피조직 과 근육조직 그리고 점액성 섬유조직에서 약한 양성반응을 보였고, 상피세포에서 강한 양성반응을 보였으며, 근육다발을 둘러싸는 섬유성 세포에서도 강한 양성반응을 보이는 세포외 기질단백질로 추측되었다. C9O-171은 약 1000bp크기로 상피조직, 근육조직 그리고 섬유기질 조직에 널리 강한 양성반응을 나타냈으며 교원띠조직에서도 양성반응을 보이는 전사인자로 추측되었다.

• Journal of Chest Surgery
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• 제39권7호
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• pp.527-533
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• 2006

배경: 전북대학교 흉부외과에서 1984년 5월 이후 시행한 St. Jude 기계판막을 이용한 승모판막 치환술의 장기 성적을 후향적 방법으로 고찰해보고자 한다. 대상 및 방법: 1984년 5월부터 1996년 12월까지 전북대학교 흉부외과에서 St. Jude 기계판막을 이용하여 승모판막 치환술을 받은 총 95명의 환자를 대상으로 하였으며 추적관찰 기간은 2004년 5월까지였다. 결과: 환자의 나이는 19에서 69세의 범위를 보였다. 평균 추적관찰 기간(mean${\pm}$standard deviation)은 $10.6{\pm}4.2$ 년이었다. 조기 사망은 4.2% (4/95) 이고 만기 사망 환자는 9명이었으며 5년, 10년, 20년 총 생존율은 각각 $90.5{\pm}3.0%,\;87.9{\pm}3.4%\;83.2{\pm}4.6%$를 보였으며 판막관련사망이 없을 확률은 각각 $95.5{\pm}2.1%,\;94.3{\pm}2.4%\;91.0{\pm}3.9%$를 보여주었다. 7명의 환자에서 혈색전층으로 인한 합병증이 발생하였고(1.05%/patient-year) 15명의 환자에게서 의의 있는 항응고제관련 출혈이 발생하였다(3.56%/patient-year). 추적관찰 기간 중 기계판막의 구조적 결함은 없었으며 모든 합병증이 발생하지 않을 확률은 5년, 10년, 20년 동안에 각각 $82.0{\pm}3.9%,\;71.3{\pm}4.8%\;42.4{\pm}10.5%$였다. 결론: St. Jude기계판막은 내구성이 좋으며 판막에 관련된 합병증의 빈도가 낮아 안전하게 사용할 수 있는 판막이라 생각하며 다만 기계판막을 가지고 있는 환자에서 항응고제 복용과 용량 조정에 관한 적정한 추적관찰로 항응고제 관련 출혈의 빈도를 더욱 낮추어야 한다고 생각한다.}3mm$였다. 괴사된 부위에서 살아 있는 세포를 발견할 수 없었다. 결론: 냉동 후 해동과정은 폐조직의 전도율을 변화시키며 이는 이차 냉동 시 폐조직의 온도를 더욱 떨어뜨리고 세포파괴의 범위를 넓힌다., $3{\mu}mol/L,\;6{\mu}mol/L,\;9{\mu}mol/L$의 농도로 첨가하여 심근세포로의 분화를 유도하였다. 4주간의 배양이 끝난 후 각 실험 조직군의 세포들을 cell blocks으로 만든 후에, CD34, heavy myosin chain, troponin T, SMA에 대한 항체를 이용하여 면역형광염색을 시행하였고, 형광염색에 반응을 보인 세포들의 대략적 비율을 비교하였다. 결과: CD34, heavy myosin chain에 대하여는 모두 음성이었으나, SMA에 대해서는 $3{\mu}mol/L,\;6{\mu}mol/L$의 복강 내 지방조직에서 $5{\sim}10%$ 정도의 양성반응을 보였고, troponin T에 대하여는 피하지방조직과 복강 내 지방조직 모두에서 $6{\mu}mol/L$와 $9{\mu}mol/L$에서 $10{\sim}15%$ 정도의 양성 반응을 보였다. 결론: 본 연구 결과, 지방조직에서 추출한 중간엽 줄기세포는 심근세포로 분화할 수 있는 가능성이 충분히 있는 것이 확인되었으나, 분화유도 비율면에서는 만족할 수준이 되지 못하였으며, 심근세포로의 분화는 복부 피하지방과 복강 내 지방조직에서 모두 가능한 것으로 판단되었다. 대형조각 작품들 - 예를 들어 대형화된 미니별 조각이나 개념미술, 또는 대지예술 등 -은 풍경의 실재가 아니기 때문에 환경으로부터 구분된다고 언급하고 있다. 이들 조각은 더 이상 만져지는 실체이거나 점유하는 공간의 상징언어를 지닌 조각의 범주에 한정되지 않게 된다.

• 운동영양학회지
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• 제24권2호
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• pp.11-21
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• 2020

[Purpose] Doxorubicin (DOX) is a potent anti-cancer drug that appears to have severe myotoxicity due to accumulation. The skeletal muscle has a regeneration capacity through satellite cell activation when exposed to extracellular stimulus or damage. Endurance exercise (EXE) is a therapeutic strategy that improves pathological features and contributes to muscle homeostasis. Thus, this study investigated the effect of EXE training in mitigating chronic DOX-induced myotoxicity. [Methods] Male C57BL/6J mice were housed and allowed to acclimatize with free access to food and water. All the mice were randomly divided into four groups: sedentary control (CON, n=9), exercise training (EXE, n=9), doxorubicin treatment (DOX, n=9), doxorubicin treatment and exercise training (DOX+EXE, n=9) groups. The animals were intraperitoneally injected with 5 mg/kg/week of DOX treatment for 4 weeks, and EXE training was initiated for treadmill adaptation for 1 week and then performed for 4 weeks. Both sides of the soleus (SOL) muscle tissues were dissected and weighed after 24 hours of the last training sessions. [Results] DOX chemotherapy induced an abnormal myofiber's phenotype and transition of myosin heavy chain (MHC) isoforms. The paired box 7 (PAX7) and myoblast determination protein 1 (MYOD) protein levels were triggered by DOX, while no alterations were shown for the myogenin (MYOG). DOX remarkably impaired the a-actinin (ACTN) protein, but the EXE training seems to repair it. DOX-induced myotoxicity stimulated the expression of the forkhead box O3 (FOXO3a) protein, which was accurately controlled and adjusted by the EXE training. However, the FOXO3a-mediated downstream markers were not associated with DOX and EXE. [Conclusion] EXE postconditioning provides protective effects against chronic DOX-induced myotoxicity, and should be recommended to alleviate cancer chemotherapy-induced late-onset myotoxicity.

• 한국가금학회지
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• 제42권1호
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• pp.27-32
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• 2015

In this study, we used two breeds of chicken to identify genomic regions corresponding to necrotic enteritis (NE) resistance. We scanned the genomes of a resistant and susceptible line of Fayoumi and White Leghorn chickens (20 birds/line) using a chicken 60 K Illumina SNP panel. A total of 235 loci with divergently fixed alleles were identified across the genome in both breeds; particularly, several clusters of multiple loci with fixed alleles were found in five narrow regions. Moreover, consensus 15-SNP haplotypes that were shared by the resistant lines of both breeds were identified on chromosomes 3, 7 and 9. Genes responsible for NE resistance were identified in chicken lines selected for resistance and susceptibility. Annotation of the regions spanning clustered divergently fixed regions revealed a set of interesting candidate genes such as phosphoinositide-3-kinase, regulatory subunit 5, p101 (PIK3R5) and inositol 1,4,5-trisphosphate receptor 1 (ITPR1), which participate in immune response. Consensus haplotypes were found in regions containing possibly relevant genes, such as myostatin and myosin, which play important roles in muscle development. Thus, genome scans of divergent selection in multiple chicken lines and breeds can be used to identify genomic regions associated with NE resistance.

• Journal of Oral Medicine and Pain
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• 제13권1호
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• pp.61-69
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• 1988

The study of the muscle fiver composition and the muscle fiver type conversion during unilateral edentulous condition was undertaken in the rostral superficial masseter muscle of the whiter rat. 16 4-week-old male white rats weighting approximately 130gm that crowns of left upper and lower molare were cut intentionally, were divided into 4 groups (one control group and 3 experimental groups). After experimental groups were sacrificed by etherization in 6 days($E_1$), 18 days($E_2$) and 36 days($E_3$) separately, samples of the rostral superficial masseter muscle were obtained bilaterally and the proportion of type I, type IIA, type IIB, and type IIC fibers was determined and counted according to their histochemical activity of myosin ATPase (at pH 9.4, pH 4.6, and pH 4.2)and PAD staining. The obtained results were as follows : 1. The rostral superficial masseter muscle of the white rat contained approximately 47.5% type I fiber and 52.5% type II fiber. 2. Type I/ Type II ratio of molar-present side was increased significantly in the group E2 (18 days group) 3. Type IIA fiber was increased at molar-present side and decreased at molar-absent side in experimental groups.

• 한국수산과학회지
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• 제40권6호
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• pp.337-342
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• 2007

The formation of lysinoalanine (LAL) in protein recovered from the belanger's croaker, Johnius grypotus, using a pH shifting process was measured by amino acid analysis. The LAL peak was detected at 49.24 min, between phenylalanine and histidine peaks in the amino acid analyzer. LAL was not detected in the fish muscle or in protein recovered using the alkaline pH shifting process. LAL was not formed in protein recovered after storage for up to 9 hrs at pH 11, but was detected in the soluble protein fraction at pH 11, followed heating at $90\;^{\circ}C$. The myosin heavy chain decreased with storage time at pH 11. The results suggest that the alkaline shifting process for recovering fish muscle protein is safe, and that no LAL forms.

• Molecules and Cells
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• 제38권9호
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• pp.781-788
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• 2015

Mutations of MYO15A are generally known to cause severe to profound hearing loss throughout all frequencies. Here, we found two novel MYO15A mutations, c.3871C>T (p.L1291F) and c.5835T>G (p.Y1945X) in an affected individual carrying congenital profound sensorineural hearing loss (SNHL) through targeted resequencing of 134 known deafness genes. The variant, p.L1291F and p.Y1945X, resided in the myosin motor and IQ2 domains, respectively. The p.L1291F variant was predicted to affect the structure of the actin-binding site from three-dimensional protein modeling, thereby interfering with the correct interaction between actin and myosin. From the literature analysis, mutations in the N-terminal domain were more frequently associated with residual hearing at low frequencies than mutations in the other regions of this gene. Therefore we suggest a hypothetical genotype-phenotype correlation whereby MYO15A mutations that affect domains other than the N-terminal domain, lead to profound SNHL throughout all frequencies and mutations that affect the N-terminal domain, result in residual hearing at low frequencies. This genotype-phenotype correlation suggests that preservation of residual hearing during auditory rehabilitation like cochlear implantation should be intended for those who carry mutations in the N-terminal domain and that individuals with mutations elsewhere in MYO15A require early cochlear implantation to timely initiate speech development.

• 한국식품영양과학회지
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• 제2권1호
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• pp.1-21
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• 1973

The muscle of abalone, Notohaliotis discus (REEVE), and top-shell, Turbo cornutus Solander, were examined for protein composition. Then paramyosins which are known as one of the important structural protein of the muscle fibrils were isolated from the both muscle and their physico-chemical properties such as solubility, salting-out behaviour, intrinsic viscosity, ATPase activity, etc. involving amino acid composition and N-terminal amino acid residues were investigated to elucidate phylogenie characteristics more intensively from the viewpoint of comparative biochemistry. The analysis of protein composition resulted in the following estimations: abalone muscle; water-soluble protein of 22 %, salt-soluble protein, 34%, alkali-soluble protein, 20%, and stroma protein, 24%, and top-shell muscle; water-soluble protein of 16%, salt-soluble protein, 30%, alkali-soluble protein, 29%, and stroma protein, 25%, respectively. It is demonstrated in sedimentation analysis that paramyosin and myosin-actomyosin account for approximately 65% and 35% of the salt-soluble protein of abalone, and that the composition of both sediments in top-shell was approximately 70% and 30%, respectively. The ultracentrifugally homogenous paramyosins isolated essentially according to Bailey's ethanol-dried method from both of the muscle showed a $S^{\circ}_{20,w}$ of 3. 14s for abalone and a $S^{\circ}_{20,w}$ of 3.50s for top-shell. The both paramyosins were commonly rich in arginine, aspartic acid, and glutamic acid, while scarcely contained proline and tryptophan, in rough accord with the other paramyosins thus far reported. It is clear that these gastropod paramyosins showed of having the characteristic N-terminal amino acid residues such as N-aspartic acid, N-valine, N-serine, and N-threonine in common. The abalone paramyosin completely salted in with KCl beyond $0.35{\mu}$ and the top-shell paramyosin beyond $0.30{\mu}$. The abalone paramyosin was salted-out between 18% and 30% saturation of ammonium sulphate and the top-shell paramyosin between 22% and 29% saturation. The intrinsic viscosities at abalone and top-shell paramyosins at $25^{\circ}C$ were estimated respectively to be 3.1 dl/g and 2.6 dl/g showing somewhat higher than the values for some other paramyosins from lamellibranchs. In regard with the ATPase activity, the para myosin specimens did not exhibit any significant activity over through the pH conditions of 5 to 9.5. irrespective of the presence of $Ca^{++}$ or $Mg^{++}$. So was the case with the abalone paramyosin prepared by a slightly modified Bailey's wet-extraction method.

• 한국수산과학회지
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• 제5권1호
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• pp.29-38
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• 1972

복족류 근육단백질을 다른 연분동물의 그것과 비교생화학적으로 검토하고져 전복을 선정하여 근육단백질의 조성을 측정하고 그 주요 구성단백질인 paramyosin을 정열 단리하여 몇가지 생물물리화학적인 성질에 관하여 실험하였다. 전복근육의 단백조성은 수용성구분 $19\~22\%$, 염용성구분 $27\~39\%$, 알카리 가용성구분 $20\~26\%$, 그리고 stroma $20\~28\%$이었다. 염용성구분은 초원심분석에서 Paramyosin이 약 $65\%$, actomyosin이 약 $30\%$, myosin이 약$5\%$로서 이루어져 있음을 알았다. 그리고 초원심분석상 균질의 단일 표품으로 판명 분리된 본 실험에서의 전복 Paramyosin은 침항정수($S^{\circ}\;_{20,\;{\omega}$) 3.14s, 정전염용 $0.35{\mu}$ 이상, $25^{\circ}C$에서의 고유점도는 3.1이었고, 한편 동 Paramyosin은 염석분석에서 이 단백질의 염석절위가 $18\~30\%$이었다. 그리고 아미노산 분석결과, 구성아미노산은 arginine, aspartic acid, glutamic acid등이 많이 함유되어 있고, proline과 tryptophane이 흠여되어 있는 점 등 2매패류의 아미노산조성과 비슷하였으나, Iysine/arginine의 비는 0.61로서 2매패류보다 낮았다.