• Mycobiology
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• v.36 no.4
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• pp.236-241
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• 2008

The colonization of an arbuscular mycorrhizal fungus Glomus intraradices BEG110 in the soil caused a decrease in disease severity in cucumber plants after fungal inoculation with Colletotrichum orbiculare. In order to illustrate the resistance mechanism mediated by G. intraradices BEG110, infection patterns caused by C. orbiculare in the leaves of cucumber plants and the host cellular responses were characterized. These properties were characterized using transmission electron microscopy on the leaves of cucumber plants grown in soil colonized with G. intraradices BEG110. In the untreated plants, inter- and intra-cellular fungal hyphae were observed throughout the leaf tissues during both the biotrophic and necrotrophic phases of infection. The cytoplasm of fungal hyphae appeared intact during the biotrophic phase, suggesting no defense response against the fungus. However, several typical resistance responses were observed in the plants when treated with G. intraradices BEG110 including the formation of sheaths around the intracellular hyphae or a thickening of host cell walls. These observations suggest that the resistance mediated by G. intraradices BEG110 most often occurs in the symplast of the host cells rather than in the apoplast. In addition, this resistance is similar to those mediated by biotic inducers such as plant growth promoting rhizobacteria.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.1
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• pp.85-88
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• 1987

Quercus acutissima seedlings were grown for two years in fumigated or non-fumigated in nursery soil in a medium with and without vegetative inoculum of the ectomycorrhizal fungi, Pisolitlius tinctorius (Pt) and Rhizopogon rubescens (Rr). Mycorrhizal formation were 42% in fumigated Pt inoculation and 36% in fumigated Rr inoculation. $2.16{\ell}\;per\;m^2$ of Pt vegetative inoculum in fumigated soil stimulated the seedling height (98%), root collar diameter (132%) and weight (420%). And Rr inoculation in fumigated soil increased the seedling height (44%), root collar diameter (23%) and weight (157%) compared with non-treated plot. There was no effect of Pt and Rr inoculation on the growth in non-fumigated soil. Nitrogen and $P_2O_5$ contents in foliage were not different by the treatment but $K_2O$ and Ca in fumigated soil were higher than non-fumigated soil.

• Applied Biological Chemistry
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• v.27 no.1
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• pp.40-44
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• 1984

Experiments on the GA production ability by ectomycorrhizal fungus, Pisolithus tinctorius was carried out to investigate specific physiological phenomena of growth increase in host plants by formation of mycorrhizae, The culture extract of P. tinctorius was purified by solvent fractionation, sephadex LH-20 chromatography, silica gel partition chromatography and TLC, successively. GA activities in the purified GA fractions were monitored by micro-drop bioassay using dwarf rice seedlings, 'Tan-ginbozu'. $30{\sim}60%$ EtOAc election fractions of silica gel pardon chromatography and the zone of Rf $0.1{\sim}0.4,\;0.6{\sim}0.8$ of TLC exhibited the GA-like activities. The GA activities were increased with the more treated amount of culture extracts. This activity in 100ml of culture solution was equivalent to 0.1ng of $GA_3$.

• Journal of Forest and Environmental Science
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• v.19 no.1
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• pp.99-107
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• 2003

For the investigation of the potentials of ectomycorrhizal (ECM) fungi on pine seedlings for reforestation on fire-damaged forest lands, six months old seedlings of Pinus densiflora, which were artificially inoculated or non-inoculated with Pisolithus tinctorius, were transplanted into the fire-damaged forest land. Seedling mortality was recorded as 3.5% for the seedlings inoculated with P. tinctorius at three months after transplanting, while it was 70.5% for the non-inoculated seedlings. Mycorrhizal root formation was shown as typically Y-branched type, and thier rate was 82% for inoculated seedlings, but it was 52% for non-inoculated. Comaprisons in seedling height, fresh weight, and dry weight between pine seedlings inoculated or non-inoculated with P. tinctorius at every 3 months till 9 months after transplanting showed that the inoculated seedlings were much better in all criteria as compared to the non-inoculated at the level of 63%, 35%, 18% in seedling height, 206%, 114%, 70% in fresh weight, and 187%, 109%, 63% in dry weight, respectively. The percentages were decreased by the elapse of time, which indicate that P. tinctorius give a growth-stimulating effects on seedlings at the early stage. T/R ratio for inoculated and non-inoculated seedlings after 9 months was 1.75 and 1.90, respectively.

• Journal of Korean Society of Forest Science
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• v.87 no.2
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• pp.270-275
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• 1998

The management of pine-mushroom forest means the environmental control for fairy ring of Tricholoma matsutake. Thus, the management demands intensive study for keeping healthy condition of the fairy ring, and that for the measurement of active portion of the soil ecosystem. This study was conducted to investigate the impact of T. matsutake fungus on the soil physicochemical properties and dehydrogenase activity by dividing the fairy ring into 3 regions such as 'zone of decayed mycorrhizae', 'zone of physiologically active mycorrhizae', and 'in front of fairy ring'. The passing of T. matsutake did not result in significant changes in canon contents of soils, but available phosphorus, carbon, and nitrogen contents were different between the soils of active mycorrhizal zone and that in front of fairy ring. The dehydrogenase activity around the fairy ring of T. matsutake was quite lower than that in general forest soils, which indicated that the fairy ring of T. matsutake was built up in the relatively immature soils which contain little organic matter. Thus, the dehydrogenase activity of soil was thought to be used as an index for the management of pine-mushroom forest with considering that the management practically means the environmental control for keeping good conditions for the development of fairy ring of T. matsutake. Especially, the dehydrogenase activity measurement can be recommended as a tool for time-decision of litter removal by floor raking since the activity is a good index of litter decomposition.

• Journal of Korean Society of Forest Science
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• v.88 no.4
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• pp.454-461
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• 1999

The dynamics of root system in the fairy rings of Tricholoma matsutake was investigated in four zones divided into 'zone of decayed mycorrhizae', 'zone of mycorrhizae for fruiting', 'zone of physiologically active mycorrhizae' and 'zone of roots free from mycorrhizal infection'. The roots of Pinus densiflora in fairy rings, which occupied 70% of upper crown, comprised about 60% and 87% of total roots and alive roots, respectively. The ratio of fine-roots of P. densiflora over total roots was relatively higher than other species since that of P. densiflora was about 45% while that of the other species was about 13% on research of fine-roots. Especially, the roots of pine comprised about 70% of total root in the zone of mycorrhizae for fruiting and the zone of physiologically active mycorrhizae, which indicated that the pine roots were closely related to the fairy rings of T. matsutake. The fine roots of P. densiflora in the zone of physiologically active mycorrhizae was about 60.7%(1,087mg/100g soil) which was about twice compared to that of other zones. It allowed us to suppose that the fine roots of P. densiflora can make active growth in the zone of physiologically active mycorrhizae, and the growth was promoted by the fairy ring formation of T. matsutake. In addition, we found 3~5 times higher amount of fine roots than that of medium roots of P. densiflora in this zone, which indicated that the fairy rings of T. matsutake locate in the region of active growth of P. densiflora' roots.

• Korean Journal of Plant Taxonomy
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• v.47 no.4
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• pp.289-296
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• 2017

The three self-compatible, terrestrial orchids Cephalanthera erecta, C. falcata, and C. longibracteata flower synchronously in sympatric populations. Cephalanthera erecta and C. longibracteata, which have white flowers that do not fully open, are predominantly autogamous, whereas the food-deceptive C. falcata, whose bright yellow flowers open completely, is predominantly outcrossing. The formation of hybrids rarely occurs between species owing to strong prepollination barriers (floral isolation). If these three species have evolved toward the prevention of interspecific hybridization, we can expect that the spatial distribution patterns of the three species would be characterized as spatial segregation (i.e., 'spatial repulsion') from each other. To test this prediction, we studied the three Cephalanthera species in sympatric populations showing coincident flowering within Yeonwhasan Provincial Park (YPP, Gyeongsangnam Province, South Korea). We found strong spatial aggregation in each population and spatial independence in the interspecific spatial distribution, differing from previous studies. We further hypothesize that Cephalanthera species in sympatry within YPP are distributed somewhat randomly in space, perhaps due to the sharing of similar mycorrhizal fungi.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.155-165
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• 2016

Domestic and international production of Tricholoma matsutake has decreased owing to matsutake forests being left alone, host plant disease, forest fires, climate change, and so on. In order to identify strains that are suitable for the production of T. matsutake-inoculated seedlings, Pinus densiflora seedlings were inoculated with T. matsutake after in vitro rooting and mycorrhization was examined in the roots of T. matsutake-inoculated seedlings after 6 months. The mycorrhization rate was greater than 80% for 5 strains (NIFoS 421, 434, 1681, 1984, and 2001) out of 19 total strains. Seven strains (NIFoS 434, 441, 561, 562, 1016, 1807, and 1812) showed shoot/root ratios of less than 3.0 and had a seedling shoot biomass of 2.0 to 4.8 times higher than that of the root. Eight strains (NIFoS 441, 561, 562, 1016, 1807, 1812, 1984, and 2001) stimulated increases in shoot volume and three stains (NIFoS 441, 562, and 1812) promoted the growth of root biomass by mycorrhizal formation. In conclusion, 4 strains (NIFoS 434, 561, 1984, and 2001) out of 19 total strains tested showed higher mycorrhization rates and seedling growth than those of the other strains. We expect that the use of these four strains may contribute to T. matsutake-inoculated seedling production.

• Journal of Korean Society of Forest Science
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• v.78 no.2
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• pp.160-167
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• 1989

This study was carried out to examine the mycorrhizal development, growth, and nutrient uptake of Quercus serrate seedlings while they were grown for 120 days in a pot medium with and without Pisolithus tinctorius, and also with applications of nitrogen levels. The results were summarized as follows : 1) Inoculated seedlings showed ectomycorrhizal formation of 61. 75%. 2) In the root development, the best nitrogen level was $300{\mu}g/ml$ for inoculated seedlings, and the number of short roots, the number of primary lateral roots and total length of lateral roots were 34.3, 30.7, and 17.2% greater, respectively, than noninoculated seedlings. 3) Growth of shoot, leaf area, total dry weight, and volume of inoculated seedlings were increased 26.9, 52.3, 31.7, and 85.7% greater respectively, than noninoculated seedlings. 4) Inoculated seedlings were more enhanced in the uptake of N, P, K, and Ca than noninoculated seedlings, and the enhanced uptake was best shown at $300{\mu}g$ N/ml treatment.

• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.429-444
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• 1998

Objective of this study was to select ectomycorrhizal fungi for black spruce(Picea mariana) inoculation to overcome the growth inhibitory effects of Kalmia angustifolia. Nineteen isolates representing 11 species of ectomycorrhizal fungi were tested for their abilities to grow and form mycorrhizae with black spruce seedlings in the presence of water leachate of leaves of Kalmia. Mycelium growth of 9 isolates were inhibited by the leaf leachate. Colony diameter and biomass of the other 10 isolates were either increased or unaffected under the same conditions. Acidic pH of the culture medium(pH 3 and 4) inhibited some of the fungi, but a combination of acidic pH and the leaf leachate was more inhibitory. Thirteen isolates were able to form ectomycorrhizae with black spruce in presence of 25% leaf leachate in pure culture. Four isolates, Paxillus involutus(NF4), Cenococcum geophilum(GB12), Laccaria laccata(GB23), and E-strain(GB45) formed mycorrhizae more successfully than the others in presence of up to 50% Kalmia leaf leachate. Black spruce seedlings pre-inoculated with these fungi were grown with Kalmia leaf leachate and live Kalmia plants during a four month greenhouse experiment. Abundant mycorrhizae(77-91% of root tips) were developed on seedlings pre-inoculated with P. involutus, L. laccata and E-strain but relatively poor mycorrhization(32% of root tips) resulted with C. geophidum. Over 90% of the short root mycorrhizae were attributed to the inoculated fungi although indigenous mycorrhizae also occurred on most seedlings. Persistence of the mycorrhizae was not affected by living Kalmia plants. Over 80% of the mycorrhizae on seedlings inoculated with P. involutus, L. laccata and E-strain and 53% of the mycorrhizae on seedlings inoculated with C. geophilum were attributable to the inoculant fungi. Control seedlings formed about 45% ectomycorrhizal short roots with indigenous fungi. The L. laccata and C. geophilum inoculated seedlings exhibited enhanced mycorrhizae formation in presence of Kalmia leaf leachate. Mycorrhizae formation with inoculant fungi was 4-15% lower at pH 4 than at pH 5, with the greatest inhibition occurring for L. laccata. Seedlings inoculated with P. involutus had the greatest shoot and root growth followed by L. laccata and E-strain inoculated seedlings. The P. involutus and L. laccata inoculated seedlings were significantly taller with more shoot dry biomass than the uninoculated(control) seedlings. E-strain inoculated seedlings had significantly higher shoot dry biomass and significantly lower number of first order lateral roots compared to the control but other growth parameters such as height, root dry weight and number of short root tips were not significantly different from the control. Seedlings inoculated with C. geophilum were not significantly different from the uninoculated seedlings in any of the growth parameters except for the number of first artier lateral roots which was significantly less than the control seedlings.