• Title/Summary/Keyword: mycological characteristics

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Mycological Characteristics and Pathogenicity of Fusarium oxysporum Schlecht. emend. Snyld. & Hans. Causing Stem Rot of Cactus (접목선인장 줄기썩음병균, Fusarium oxysporum Schlecth. emend. Snyd. & Hans.의 균학적 특성과 병원성)

  • 현익화;이상덕;이영희;허노열
    • Korean Journal Plant Pathology
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    • v.14 no.5
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    • pp.463-466
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    • 1998
  • A Fusarium species was isolated from stems of cactus(Hylocereus trigonus) showing rot symptoms at Koyang, Kyonggi province in 1997. This pathogen was identified as Fusarium oxysporum based on mycological characteristics. The rot symptom appeared at the soil line and roughly circular lesions, 1∼3 mm in diameter, appeared on basal stems. The pathogen formed both microconidia and macroconidia. Microconidia were formed abundantly in false-heads on short monophialides, oval to kidney-shaped. Macroconidia were slightly sickle-shaped, 3∼5-septated with an attenuated apical cell and a foot-shaped basal cell. Colony color on PDA was white, peach or purple. Chlamydospores were formed abundantly on PDA. The pathogen was able to cause stem rot symptoms to cactus by wound inoculation as well as non-wound inoculation.

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Mycological Characteristics and Carbon Source Activities of Unrecorded Wild Yeast Species from Vineyard Soil in Korea (국내 포도밭 토양에서 분리된 야생 효모 미기록종들의 균학적 특성과 탄소원 활성)

  • Bong Soon Yim;Myung Kyum Kim
    • The Korean Journal of Mycology
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    • v.51 no.4
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    • pp.373-381
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    • 2023
  • This study aimed to isolate and characterize wild yeast strains from the vineyard soil in Korea. Twenty yeast strains were isolated from vineyard soil in Gimpo-si, Gyeonggi-do, Korea, where Vitis labrusca cv. Campbell Early is grown. Eighteen strains were previously recorded in Korea. The remaining two, Cyberlindnera mrakii VG-21-10C and Starmerella bacillaris GR9 were not previously recorded in Korea. The mycological characteristics of VG-21-10C and GR9 were investigated. Both were oval-shaped, convex, and smooth. However, differences were evident in colony color and carbon assimilation activities. Strain VG-21-10C is white-colored and assimilates glucose, glycerol, D-xylose, D-cellobiose, D-saccharose, and D-raffinose as carbon sources. Strain GR9 is cream-colored and assimilates glucose, D-saccharose, and D-raffinose as carbon sources.

Bisifusarium Delphinoides, an Emerging Opportunistic Pathogen in a Burn Patient with Diabetes Mellitus

  • Park, Ji-Hyun;Oh, Junsang;Song, Ji-Sun;Kim, Jayoung;Sung, Gi-Ho
    • Mycobiology
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    • v.47 no.3
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    • pp.340-345
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    • 2019
  • An 82-year-old man with diabetes was admitted to the emergency department with a third-degree burn on his left leg. The deep swab specimen from his left leg was cultured on Sabouraud dextrose agar without cycloheximide and incubated at $25^{\circ}C$ for 5 days. On the basis of morphological characteristics and multigene phylogenetic analyses of the internal transcribed spacer region of ribosomal DNA and partial fragments of beta-tubulin and translation elongation factor 1-alpha, the causal agent of fungal skin infection was identified as Bisifusarium delphinoides, which was newly introduced by accommodating a Fusarium dimerum species complex. Thus, we describe here the first case of skin infection caused by B. delphinoides on a burn patient with diabetes mellitus based on morphological observation and molecular analysis.

Mycologic Characteristics of Trichophyton tonsurans (Trichophyton tonsurans의 진균학적 성상)

  • Bang, Young Jun;Kim, Ssang Young;Shin, Du Man
    • Korean Journal of Clinical Laboratory Science
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    • v.36 no.1
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    • pp.7-12
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    • 2004
  • Trichophyton(T.) tonsurans has been identified for the first time in Korea in 1995 and has been increasing in number of reported in wrestlers, in judo players, and in korean - style wrestlers. Author tried mycological studies with T. tonsurans is isolated in Daegu Korea. The results are as follows: All 16 strains of T. tonsurans tested for nutritional requirement, showed strongly stimulated growth by thiamine, mild to moderate growth on casamino acid agar, poor growth on ammonium nitrates base agar. All 16 strains of T. tonsurans tested showed positive results for hair perforation and urease test. Gross findings of T. tonsurans showed that fine granular surface with light yellow hue or white color and mahogany brown color on the reverse side. Microscopic findings showed that septated hyphae, chlamydoconidia and microcondia. Microconidia were characteristic in their arrangerment and shape : round or oval shaped microconidia laterally to hyphae, some were match - head like terminal swelling.

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Identification and Antifungal Susceptibility Profiles of Cyberlindnera fabianii in Korea

  • Park, Ji-Hyun;Oh, Junsang;Sang, Hyunkyu;Shrestha, Bhushan;Lee, Hyeyoung;Koo, Jehyun;Cho, Sung-Il;Choi, Ji Seon;Lee, Min-Ha;Kim, Jayoung;Sung, Gi-Ho
    • Mycobiology
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    • v.47 no.4
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    • pp.449-456
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    • 2019
  • Invasive fungal infections caused by Cyberlindnera fabianii have recently increased. However, biochemical kits such as API 20 C AUX and Vitek-2C have misidentified this species as other Candida spp. such as C. pelliculosa or C. utilis due to no information of Cy. fabianii in yeast database. During our 2016-2017 surveys, eleven isolates of Cy. fabianii were obtained in International St. Mary's Hospital in Korea. Here, we describe its morphological and molecular characteristics and tested its antifungal susceptibility against nine antifungal agents. The sequences of the ITS region and the D1/D2 region of LSU revealed 100% identity with the sequences of Cy. fabianii. In comparison with the results from MALDI-TOF mass spectrometry, we found that Cy. fabianii can be distinguished from other species. In antifungal susceptibility test, voriconazole and echinocandins exhibited good antifungal activities against the majority of Cy. fabianii isolates despite the absence of standard criteria.

Botrysphaeria dothidea, the Causal Organism of Ripe Rot of Kiwifruit (Actinidia deliciosa) in Korea

  • Koh, Young-Jin;Lee, Jae-Goon;Lee, Dong-Hyun;Hur, Jae-Seoun
    • The Plant Pathology Journal
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    • v.19 no.5
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    • pp.227-230
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    • 2003
  • Ripe rot was commonly found in overripe kiwifruits (Actinidia deliciosa) during a disease survey conducted in Jeju and Jeonnam provinces in Korea in 1999. While the disease did not manifest any clear external symptoms on kiwifruits, it caused portion of the fruit surface to collapse. Watersoaked flesh tissue could be seen on the sunken part when the skin of the collapsed portion was peeled off. The milky internal symptom with dark green margin developed concentrically as the fruit ripened. A species of Botryosphaeria was consistently isolated from lesions showing typical symptoms of ripe rot on kiwifruit, and its pathogenicity was confirmed by artificial inoculation test on healthy kiwifruits. The fungus was also pathogenic to apple and pear. The mycological characteristics of the fungus were consistent with those of Botryosphaeria dothidea. This is the first report on the detailed mycological characteristics of the causal organism of post-harvest ripe rot of kiwifruit in Korea.

Mycological Characteristics of Ophiostoma quercus, a Sap-staining Fungus Isolated from Japanese Black Pine in Korea (국내 해송에서 분리한 변색균 Ophiostoma quercus의 균학적 특성)

  • Hyun, Min-Woo;Suh, Dong-Yeon;Yun, Yeo-Hong;Kim, Seong-Hwan
    • The Korean Journal of Mycology
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    • v.36 no.2
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    • pp.123-129
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    • 2008
  • One of the sapstain fungal species isolated from the stained sapwood of a Japanese black pine in Korea was characterized. The fungal species is tolerant to cycloheximide and has Petosum-like synnema and Sporothrix type synanamorph which are found in the anamorphs of the Ophiostoma piceae complex group. But the species could not form perithecia on MEA. Based on cultural and morphological properties and analysis of the $\beta$-tubulin gene sequence, the fungal species was identified as Ophiostoma quercus. Here, we report mycological characteristics of the anamorphic stage of Ophiostoma quercus isolated in Korea.

Pathogenicity and Mycological Characteristics of Pythium myriotylum Causing Rhizome Rot of Ginger (생강뿌리썩음병균 Pythium myriotyrum의 병원성 및 균학적 특성)

  • Kim, Choong-Hoe;Yang, Sung-Seok;Park, Kyong-Seok
    • Korean Journal Plant Pathology
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    • v.13 no.3
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    • pp.152-159
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    • 1997
  • Six pathogenic Pythium isolates obtained from diseased ginger rhizomes were identified as Pythum myriotyrum Drechsler based on various morphological and physiological characteristics. The isolates showed strong virulence on underground parts of buds, crowns, rhizomes, roots and aerial parts of leaves and stems as well. The isolates caused rot of germinated seeds of 10 different crops tested, including cucumber and pepper, and markedly inhibited seedling growth of 3 crops tested, including corn and barley. Maximum, optimum and minimum growth temperatures for P. myriotylum were 39~45$^{\circ}C$, 33~37$^{\circ}C$ and 5~7$^{\circ}C$, respectively. Optimum pH for the growth was 6~7. Mycelial linear growth was most rapid on V-8 juice agar, but aerial mycelia were most abundant on PDA and corn meal agar. Zoosporangial and oogonial formation was greatest on V-8 juice agar. Optimum temperatures for the production of zoosporangia and oogonia were 20~35$^{\circ}C$ and 15$^{\circ}C$, respectively.

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