• Plant Resources
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• v.3 no.2
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• pp.110-117
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• 2000

The recycling method of enokitake cultural waste and the potentiality of second flush for enokitake were determined, because this fungus is not as prolific as the more commonly cultivated white rot fungi in the conversion of sawdust to mycelial mass. The mycelial growth of F. velutipes on several substrates, variously treated with rice bran was promoted at ratios of 10-20% (w/w) on all substrates, but suppressed at above ratios, although some difference was there. The mycelial densities generally increased correlated to the supplementation contents of rice bran. It could be concluded that F. velutipes preferred mild acidic to acidic conditions for mycelial growth, considering that the mycelial growth rate was highest on waste of pH 6.01, treated with 0.1 % Ca(OH)$_2$ and on populus mixed waste of pH 6.02, non treated. The ranges of substrate bulk densities, which was pertinent for mycelial linear growth were from B.D. (g/cc) 0.17 to 0.23 on waste and populus mixed waste all. The pertinent contents of rice bran supplementation in bottle cultivation was from 20 to 30% on waste and 20% on populus mixed waste, considering the requried duration for pinheading and fruiting yields. Standard bulk density for filling and utilizing the waste and populus mixed waste for commercial f. velutipes cultivation were B.D.(g/cc) 0.19 ~ 0.23, and 0.23~ 0.25, which could be conversed to 510~ 540g/900m1 and 520~ 570g/900m1, respectively, The second flush of F. velutipes was tried and the re-inoculation by sawdust and liquid spawn showed somewhat good results, indicating the potentiality of second crop and suggesting further research for it.

• The Korean Journal of Mycology
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• v.26 no.1 s.84
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• pp.39-46
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• 1998

This study was carried out to obtain the basic data for artificial culture of Sparassis crispa. The optimal conditions for the mycelial growth were $25^{\circ}C$ and pH 4, respectively. The mycelial density of S. crispa was most compact in the Hamada media, whereas colony diameter was prominent in the Hoppkins media. Carbon sources such as maltose, arabinose and mannitol were favorable for stimulating a mycelial growth of S. crispa. Glycine, one of nitrogen sources also appeared to be favorable to the mycelial growth. The optimum C/N ratio was about 20 : 1 in case that 1% glucose as carbon source was mixed with the basal medium. Fumaric acid or lactic acid as organic acid was most favorable to the mycelial growth.

• The Korean Journal of Mycology
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• v.11 no.3
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• pp.121-128
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• 1983

Nutritional and physio-chemical conditions for mycelial growth and fruit-body formation of Pleurotus florida were determined in synthetic media. Mannitol and sucrose were good sugar substances for the mycelial growth and fruit-body formation whereas less mycelial growth and no fruit-body formation was obtained with arabinose, lactose and inulin. The optimum concentration of mannitol was about 2%. Peptone as a nitrogen sources resulted in a rapid mycelial growth and fruit-body formation with higher yield, but nitrite-nitrogens inhibited the mycelial growth. The higher yield was obtained with 0.2% peptone. Among the vitamins used, the greatest mycelial growth and fruit-body formation brought about by thiamine and folic acid. $KH_2PO_4$ and $MgSO_4$ at 0.2% and 0.02%, respectively, were effective for the mycelial growth and fruit-body formation, but other inorganic salts used were ineffective. The optimum temperature for mycelial growth and fruit-body formation were $25^{\circ}C$ and $20^{\circ}C$, respectively, and light intensity of $100{\sim}500\;lux$ and pH 6.0 appeared to be effective.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.81-87
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• 2019

The investigation of mycelial growth for artificial cultivation in T. albuminosus determined that within PDA, YM, MEA medium, both KNCAF 9001 and 9002 strains showed the highest mycelial growth in MEA media with 89, 81 mm, respectively, followed by YM, PDA in respected order. Furthermore, optimal mycelial growth of 89 and 77 mm in 9001 and 9002, respectively, at 25 ℃. Mycelial growth rapidly decreased at 30 ℃, but it was confirmed that it survived at 40 ℃. At pH 6, mycelial growth was fast with growth of 89 and 88 mm for both 9001 and 9002, respectively. In the case of 9001, the addition of with cotton seed hull(80%) and rice bran (20%) each other resulted in maximum productivity of 99 mm/10 days. The result differed in order of corn cob(97 mm), oak sawdust(96 mm) and poplar sawdust(93 mm). Ultimately, the result confirmed that addition of rice bran in comparison to bran resulted in higher yield. Even with identical medium component, 9002 showed the same tendency as 9001 but its growth was relatively slower than 9001. The effect of mycelial growth on the moisture content of the medium was best at 101. 94 mm/10 days when the moisture content of 9001 and 9002 was 65%. Mycelial growth rate tended to be slower when the moisture content was higher or lower than 65%.

• KSBB Journal
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• v.26 no.4
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• pp.357-364
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• 2011

This study was carried out to investigate the effects of agitation, aeration and scale-up on the mycelial growth, exo-polysaccharide (EPS) production, and mycelial morphology in the liquid culture of Ganoderma lucidum. A correlation between roughness and operating variables was also studied to scale-up the liquid culture of G. lucidum in a jar fermenter. When the agitation speed or aeration rate increased, the morphological form was changed from rough pellet to smooth pellet form. Increase of the agitation and aeration reduced the mycelial roughness. On the other hand, in the case of pellet size, it was not affected by aeration. The higher EPS production was obtained at approximately 17% of roughness and mycelial pellet size of 3~5 mm. The morphology at each fermenter was closely correlated with kLa value, and it was found that similarity of morphology would be used as a criteria of scale-up for liquid culture of G. lucidum.

• Mycobiology
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• v.33 no.1
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• pp.65-67
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• 2005

Cystoderma amianthinum, one of edible fungi belongs to Agaricaceae of Basidiomycota, has a good taste and flavor. This study was carried out to obtain the basic informations for the optimum mycelial growth of C. amianthinum. The optimal conditions for the mycelial growth were $25^{\circ}C$ and pH 5 in potato dextrose agar (PDA). C. amianthinum showed the favorable growth in the PDA and yeast malt extract agar (YMA). The favorable carbon and nitrogen sources promoting mycelial growth were fructose and histidine, respectively. The optimum C/N ratio was about 30 : 1 in case that 1 % glucose was supplemented to the basal medium as a carbon source.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.1067-1070
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• 2004

In the course of screening for specific inhibitors against the mycelial form of Candida albicans from natural resources, we have isolated and identified A6792-1 from Streptomyces sp. A6792 by using several chromatographies. By spectral analyses, this compound was determined as 7-oxostaurosporine, having a structure of staurosporine aglycon noiety. 7-Oxostaurosporine exhibited a selective growth inhibitory activity against the mycelial form of Candida spp. up to $100\mu\textrm{g}/disc$ in bioassay. It also exhibited a specific antifungal activity against the mycelial form of Candida spp. including C. krusei, C. albicans, C. tropicalis, and C. lusitaniae with MICs ranging from 3.1 to $25\mu\textrm{g}/ml$ 7-Oxostaurosporine demonstrated no in vivo toxicity in SPF ICR mice. Therefore, this compound may have a considerable potential as an antifungal agent based on the preferential inhibition against growth of the mycelial form of Candida spp., dimorphic fungi.

• Mycobiology
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• v.30 no.4
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• pp.208-212
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• 2002

For comparison of mycelial colonization of Phellinus linteus on logs, several techniques of inoculation were tested; sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation. The mycelial colonization of P. linteus on logs was good in the treatment of sterilized short log inoculation, but poor in the traditional methods such as drilling inoculation and log-end sandwich inoculation. The initial mycelial growth and the full mycelial colonization of P. linteus in logs were the best in case of 20 cm logs under the condition of 42% moisture content. Also, the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method of logs after $5{\sim}6$ months of incubation was the best for formation of basidiocarps of P. linteus. The formation of fruiting body of P. linteus was quite good in the cultivation house at $31{\sim}35^{\circ}C$ and over 96% of relative humidity.

• Korean Journal Plant Pathology
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• v.13 no.1
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• pp.30-36
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• 1997

The effects of carbon and nitrogen sources on the mycelial growth and sporulation of microconidia and chlamydospores of five isolates of Cylindrocarpon destructans (Zinssm.) Scholten causing root rot of Panax ginseng were studied. For the carbon sources, fructose, glucose, maltose, and sucrose in Czapek-Dox broth showed good mycelial growth of 178∼201 mg in dry weight compared with 64 mg of the control. The best carbon sources tested for conidial formation were sucrose and maltose with 2.75 and 3.03 log conidia/ml, respectively. For the nitrogen sources, aspartic acid, NaNO3, KNO3, arginine, threonine, and leucine increased mycelial growth of the fungi to 208∼231 mg in dry weight without significant difference (p=0.05) among them. Meanwhile the growth with cystine was poor (26.3 mg dry weight), and no conidium and chlamydospore were formed. Maximum microconidial formation was observed in the media with NaNO3 and KNO3 as 3.37 and 3.35 log conidia/ml, and for the chlamydospore formation the (NH4)2SO4-containing medium and the nitrogen-absent medium were the best as 3.40 and 3.57 log chlamydospores/ml, respectively. No conidium was found in the medium without nitrogen sources, in which chlamydospore formation increased 6 times more than in the nitrogen-amended medium. However, deletion of carbon source in the medium did not affect on the formation of conidia and chlamydospores of C. destructans.

• Korean Journal Plant Pathology
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• v.10 no.4
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• pp.325-332
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• 1994

In order to find out formulation and effect of soil amendment on Fusarium wilt of sesame caused by Fusarium oxysporum f. sp. vasinfectum, the study was conducted during the last two years of 1992 to 1993. Among 14 chemicals (1%, w/w) added to soil including CaO individually, Al2(SO4)3, Alum, and CaO suppressed mycelial growth and conidial germination of F.oxysporum f. sp. vasinfectum. CaCl2 suppressed mycelial growth only, while glycerine, KCl, K2 HPO4, and triple superphosphate suppressed conidial germination. Suppression rate was ranged from 21 to 100% on mycelial growth. The 8 chemicals were finally selected. Among the 4 organic compounds, composted pine bark showed definite suppression on mycelial growth and conidial germination of the fungus, whereas milled alfalfa leaves was only effective on conidial germination of Fusarium wilt pathogen. The antagonist Trichoderma harzianum grew well in the soil medium amended with the composted pine bark and chemicals mixture (CPM) amendment (1%, w/w) and suppressed mycelial growth of the fungus effectively. In pot test, Fusarium wilt of sesame was completely controlled by CPM amendment.