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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effect of Agitation, Aeration and Scale-up on Mycelial Morphology During Liquid Culture of Ganoderma lucidum

영지의 액체배양 중 균사형태에 미치는 통기.교반의 영향 및 Scale-up

  • Lee, Hak-Su (Technique Research Institute, Dongbang Future Chemical Co., Ltd.) ;
  • Lee, Ki-Young (Department of Bioengineering and Technology, Kangwon National University) ;
  • Choi, Sang-Yun (School Life Sci. Biotechnol., Korea University) ;
  • Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University)
  • 이학수 (동방미래화학(주) 기술연구소) ;
  • 이기영 (강원대학교 생물공학과) ;
  • 최상윤 (고려대학교 생명공학원) ;
  • 이신영 (강원대학교 생물공학과)
  • Received : 2011.06.15
  • Accepted : 2011.08.29
  • Published : 2011.08.30
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Abstract

This study was carried out to investigate the effects of agitation, aeration and scale-up on the mycelial growth, exo-polysaccharide (EPS) production, and mycelial morphology in the liquid culture of Ganoderma lucidum. A correlation between roughness and operating variables was also studied to scale-up the liquid culture of G. lucidum in a jar fermenter. When the agitation speed or aeration rate increased, the morphological form was changed from rough pellet to smooth pellet form. Increase of the agitation and aeration reduced the mycelial roughness. On the other hand, in the case of pellet size, it was not affected by aeration. The higher EPS production was obtained at approximately 17% of roughness and mycelial pellet size of 3~5 mm. The morphology at each fermenter was closely correlated with kLa value, and it was found that similarity of morphology would be used as a criteria of scale-up for liquid culture of G. lucidum.

Keywords

References

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