• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 추계학술대회
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• pp.133-133
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• 2020

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 춘계학술대회
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• pp.19-19
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• 2022

• The Plant Pathology Journal
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• 제35권3호
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• pp.280-286
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• 2019

In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed interactions with abiotic and biotic stress response-related rice proteins, as shown by genome-wide Y2H screening employing PVF11 and a cDNA library from B. glumae-infected rice. To confirm the effect of PVF11 on B. glumae virulence, in planta assays were conducted at different stages of rice growth. As a result, a PVF11-defective mutant showed reduced virulence in rice seedlings and stems but not in rice panicles, indicating that PVF11 involvement in B. glumae virulence in rice is stage-dependent.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.68.1-68
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• 2003

When plants are infected by plant pathogens, typical disease symptom termed lesion, appears in compatible interaction. Whereas, in incompatible interactions, only small speck of lesions are visible on the leaf surfaces. Hypersensitive response (HR) of plant which is the result of infection by incompatible pathogens, is a well known defense response inducing rapid cell death resulting in complete resistance. However, some rice mutants show spontaneous disease symptoms during the growth stages without interaction with pathogens. We investigated the spontaneous cell death mutant called Blast Lesion Mimic(BLM) generated by EMS mutation, on the relationship with the hypersensitive response as well as resistant characteristics. Accumulation of phenolic compounds were detected around the lesions as lesions develop on leaf surface. Activation of PR gene was detected before the lesion appeared, and that result indicates the defense-related response are started earlier than lesion formation. The BLM mutant showed resistant response to inoculation of Magnaporthe grisea KJ201 with which the wild type Hwacheong is totally susceptible. Informations on the formation of spontaneous lesions and detail analysis of lesion mimic mutants and related genes are very limited to date. It is really important to understand the phenomenon of the defense-related lesion formation for developing resistant cultivar for rice blast pathogens

• 한국육종학회지
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• 제42권1호
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• pp.23-27
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• 2010

The objective of this study was to map the gene for reduced embryo size in rice using DNA markers. The reduced embryo size mutant was induced from N-methyl-N-nitrosourea (MNU) treated Taichung 65. Genetic analysis revealed that the phenotype of the reduced embryo was controlled by a single recessive gene, designated as re-1. For mapping the gene controlling embryo size, an $F_2$ population was developed from a cross between the Korean Tongil-type, Milyang 23 (Oryza sativa ssp. indica) and the mutant. The ratio of $F_2$ seeds nearly fitted to 3:1 ratio, indicating that this phenotype was controlled by a single recessive gene. Bulked sergeant analysis was performed with SSR markers. The gene for the reduced embryo size was detected on chromosome 1. The gene was further mapped between two SSR markers, RM315 and RM265 on chromosome 1 (approximately 1.5 Mb interval). The linked markers will facilitate selection of this grain character in a breeding program and provide the foundation for positional cloning of this gene.

• 한국작물학회지
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• 제40권5호
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• pp.637-643
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• 1995

5-methyltryptophan (5MT) 저항성 벼 (Oryza sativa L. val. Sasanishiki)로 부터 선발된T-RI의 자식후대개체 중에서 저항성이 우성형질로 분리된 TR75주를 이용하여 그 식물체의 여러 특성을 조사하였다. 그 결과를 요약하면 다음과 같다. 1. TR75는 종자, 근, 약에 있어서는 25 mg/1의 5MT가 포함된 MS배지에서 callus가 형성되었으나, 야생형의 경우에는 어느 기관에서도 Callus 유도는 일어나지 않았다. 2. 각 기관으로 부터 유도된 TR75의 callus는 50mg/1의 5MT배지에서도 저항성을 보여주었지만 야생형의 callus는 25mg/1의 5MT 배지에서 모두 갈변화 하였다. 3. TR75는 3종류의 아미노산 아날로그(L-azetidine-2-carboxylic acid, S-2-aminoethyl-L-cystene, p-fluoro-DL-phenylalanine)에 대해서는 저항성을 갖고 있지 않다. 4. TR75의 유리아미노산 함량은 야생형 종자에 비해 tryptophan, phenylalanine, aspartic acid가 각각 5.0, 5.3, 2.7 배 증가하였으며, 그 외 다른 종류의 아미노산 함량도 1.5∼2.0배 증가하였다. 전 아미노산 함량도 약 1.5배 가량 증가하였다. 그러나 alanine과 leucine은 약간 감소하였다.

• 방사선산업학회지
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• 제6권2호
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• pp.129-138
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• 2012

Proteomic analysis was performed in order to identify proteomic changes by salt stress between the Japonica cv. Donganbyeo (WT) and two salt-tolerant (ST) mutant lines by using the SDS-PAGE and 2-DE. Two salt tolerant rice mutant lines, ST-87 and ST-301, were selected by in vitro mutagenesis with gamma-ray. Three-week-old seedlings were treated with 171 mM NaCl for 7 days. In the SDS-PAGE, three proteins with molecular weights of 27, 46 and 58 kDa were highly increased under salt treatment. Total proteins from shoots of both WT and ST-lines were separated by two-dimensional gel electrophoresis. In 2-DE, 201, 226, 217 and 213 protein spots were detected in the untreated-or treated-WT and untreated- or treated-ST-87, respectively. Of theses, 17 and 10 protein spots were up- and down-regulated under salt stress in the WT, respectively. While, 16 and 8 protein spots were up- and down-regulated under salt stress in the ST-87, respectively, compared with the untreated plants. High intensity or de novo synthesized proteins were analyzed by MALDI-TOF/MS analysis.

• 방사선산업학회지
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• 제5권3호
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• pp.259-266
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• 2011

When plants undergo stress, Reactive oxygen species (ROS) which remove bad elements such as mildew and virus is activated in plant body. However, if ROS is excessively increased, plant will be harmed itself by destruction of cell and signal system and phenomenon of lipid peroxidation. In order to identify content of lipid peroxidation and activity of some enzymes scavenging ROS, phenotypical and physiological analysis was performed with two mutant lines, Till-II-877 and Till-II-894, comparing with cv. Dongan (WT). In phenotype analysis, two mutant lines give to well-conditioned growth better than WT in since 5 days after salt treatment. In enzyme activities, there was a modest difference in the content of catalase (CAT) and peroxidase (POD) between Till-II-877 and Till-II-894, two mutant lines showed high levels in CAT contents than WT. However, they express low levels in POD contents. In MDA analysis, the content of Till-II-877 was higher than that of WT, but Till-II-894 was lower. This result indicates that two mutants have different mechanism against salt stress.

• 한국육종학회지
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• 제40권3호
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• pp.303-307
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• 2008

출수가 늦어 남해안 일부 지역에서만 재배되던 재래종 녹미(일명; 생동찰벼)를 개량하여 전국적으로 안전하게 재배할 수 있도록 신품종 "녹원찰벼"를 육성하였다. 생동찰벼의 종자에 감마선 200 Gy를 조사하여 $M_2$세대에서 출수가 빠르고 키가 작은 우량 계통을 선발하였다. 계통선발과 지역적응성 시험을 거쳐 유망계통 원농 17호(녹원찰벼)를 최종적으로 선발하여 품종등록하였다. 녹원찰벼는 생동찰벼와 비교하여 중부지방에서 이삭 피는 시기가 생동찰벼보다 15일정도 빠른 8월 17-20일경이었고 간장은 15 cm 정도 작은 91 cm이었다. 녹원찰벼의 현미 수량은 생동찰벼보다 10% 정도 증가하였다. 녹원찰벼의 백미 및 현미중의 아미노산 함량은 다른 대조 품종보다 10-60% 높았다. 엽록소 함량은 대조품종 신선찰벼 및 남평벼보다 비해 2배 높았다. 등숙기에 엽록소 소실이 늦어 녹색 현미 찹쌀로 이용이 가능하여, 일반 쌀에 섞거나 현미밥을 지으면 연한 황록색의 시각적인 효과와 고소하고 차진 특성이 있다. 병해충에도 강하여 앞으로 친환경 기능성 쌀로 이용확대가 기대된다.

• 한국식품영양과학회지
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• 제39권9호
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• pp.1313-1319
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• 2010

일품벼에서 유래된 신형질미의 취반 및 가공 등 용도개발을 위한 목적으로 백미와 현미에 대한 성분 분석 및 물리적 특성을 분석한 결과, 원품종인 일품벼과 신형질미인 고아미2호, 백진주, 설갱의 이화학적 특성은 품종 간에 유의한 차이를 보였다. 고아미2호는 고 아밀로스 품종으로 분류되며, 난소화성 다당류의 함량도 높게 나타난 반면에, 백진주는 저아밀로스 품종으로 반 찰벼의 특성을 보였다. 지방산은 백미와 현미에서 linoleic acid와 oleic acid가 전체 지방산의 70~75%를 차지하는 양질의 기름으로서, 백미는 linoleic acid가 현미에는 oleic acid 함량이 높은 경향을 보였다. 한편, 고아미2호는 palmitic acid 함량이 가장 높아서 다른 품종과 다소다른 지방산 조성을 나타내었다. 백미의 주요 아미노산은 aspartic acid, glutamic acid, cysteine, hydroxy lysine이고, 현미에서는 cysteine을 제외한 aspartic acid, glutamic acid, hydroxy lysine인 것으로 나타났다. 쌀 품종의 호화특성을 DSC로 분석한 결과, 호화에 필요한 흡열엔탈피는 반 찰성인 백진주가 가장 높고, 다음은 설갱, 일품, 고아미2호 순으로, 고아미2호가 가장 낮은 흡열엔탈피을 보였다. 취반미의 식미치는 일품벼가 가장 높았으나, 밥의 텍스쳐를 분석한 결과, 고아미2호의 경도가 가장 높고, 일품, 백진주, 설갱 순으로 경도가 낮아지는 것으로 분석되었다. 신형질미 중에서, 고아미2호 취반미는 푸석한 조직감을 보여 다른 품종과는 현저히 다른 특성을 나타내므로, 고아미2호는 취반용 쌀로서 이용하기 보다는 가공용 기능성 곡류 소재로서 활용 가능성이 높으며, 반면에 백진주와 설갱은 원품종인 일품과 비교했을 때 취반용과 가공용도에 모두 적합할 것이다.