• Journal of Mushroom
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• v.8 no.3
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• pp.116-121
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• 2010

To elucidate the effect of humidity to the characteristics related to mushroom cultivation, five white strains and four brown strains of Flammulina velutipes were cultivated and investigated on their characters. The periods for fruiting initiation, growth and harvest were a little decreased when humidity increased after peak at 75%. Brown strains showed fast fruiting than white ones. ASI 4103 was the fastest and ASI 4166 and ASI 4153 were the latest. The productivity of ASI 4166 and ASI 4149 were the best in all conditions. In general, white strains showed high yields at 75% and brown ones did at 95%. Individual mushroom weight decreased and water content of mushroom increased in most strains when humidity increased.

• The Korean Journal of Mycology
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• v.43 no.3
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• pp.174-179
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• 2015

For development a new anti-diabetic compound from edible mushroom, antihyperglycemic ${\alpha}$-glucosidase inhibitory activities of Pleurotus ostreatus, Pleurotus cornucopiae, Pleurotus salmoneostramineus, Pleurotus eryngii and Neolentinus lepideus were investigated on its water and ethanol extracts. ${\alpha}$-Glucosidase inhibitory activity of Neolentinus lepideus fruiting body showed the highest at 86.3% in the 95% ethanol extracts and water extract from Pleurotus cornucopiae was also higher at 48.5% among water extracts. Therefore, Neolentinus lepideus which showed very high ${\alpha}$-glucosidase inhibitory activity was selected as a new anti-diabetic agent-containing mushroom and the ${\alpha}$-glucosidase inhibitor was maximally extracted when treated with 95% ethanol at $30^{\circ}C$ for 48 hr. The ethanol extracts from Neolentinus lepideus fruiting body showed dosage-dependent hypoglycemic action after administration to 120 min in the SD-rat and streptozotocin-induced diabetic SD-rat.

• Journal of Mushroom
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• v.7 no.4
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• pp.168-172
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• 2009

We attempted to isolate the bacterial strains from the fruiting bodies of Agaricus brazilensis and determined their effects on browning and distortions of mushrooms. No bacterial strains were isolated when the middle of browning regions of A. brazilensis were used. Total 125 bacterial strains were obtained from the surface of browning regions and classified into 17 different genera and 29 species by using MIDI methods. Most common genus were Pseudomonas (26), Yersinia (29), and Cedecea (29). High lytic activity were detected when Pseudomonas strains were tested, while relatively low lytic activity were observed with both Yersinia and Cedecea strains.Therefore, we believed the distortion of mushroom could be the result of bacterial infections. Also, the development of brownish color was detected in large number of A. brazilensis strains only by incubation at 4C, suggesting no specific correlation between bacterial strains and brownish color development. Also, it is considered that the development of brownish color can be the normal changes of A. brazilensis.

• Journal of Mushroom
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• v.16 no.2
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• pp.86-89
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• 2018

To develop Grifola frondosa as a new commercial product, we investigated the optimal primordium formation method for bottle cultivation. Primordium formation was initiated on the sixth day in all treatments, but in the T7 treatment, which consisted of removing old spawn and turning the bottle upside-down, was initiated on the ninth day. The ratio of primordium formation was at its highest value, 98.9%, in T3 and T7. Among the treatments, T7 produced the highest quality fruiting bodies and a mushroom yield of 112.8 g in each 850 mL bottle, but its crop cycle time was somewhat longer than those of the other treatments. These results indicate that appropriate methods for primordium formation in bottle culture could enhance mushroom quality and productivity.

• Journal of Microbiology and Biotechnology
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• v.12 no.4
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• pp.691-691
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• 2002

In the course of our screening for neuroprotective agents, a new compound (1) was isolated together with a known compound, caruilignan C (2), from the fruiting body of Daldinia concentrica. Their structures were determined on the basis of various spectral studies. Both compounds exhibited neuroprotective effect against iron-induced neurodegeneration in a primary culture of mouse cortical neurons.

• Journal of Microbiology and Biotechnology
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• v.12 no.4
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• pp.692-694
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• 2002

In the course of our screening for neuroprotective agents, a new compound (1) was isolated together with a known compound, caruilignan C (2), from the fruiting body of Daldinia concentrica. Their structures were determined on the basis of various spectral studies. Both compounds exhibited neuroprotective effect against iron-induced neurodegeneration in a primary culture of mouse cortical neurons.

• Journal of Mushroom
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• v.11 no.4
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• pp.219-225
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• 2013

This study was carried out to investigated optimum mixing ratio of Korean natural Juglans mandshurica using as functional plants. Total nitrogen and carbon source of J. mandshurica was 0.21% and 46.0%, respectively and C/N ratio was 219. Total nitrogen source and pH of substrate mixed with J. mandshurica was 2.3~2.9 and 5.0, respectively. The contents of CaO, MgO and $Na_2O$ at J. mandshurica media were higher at harvest media than inoculation media. Mycelial growth was the fastest at J. mandshurica 20%, and slower by increase of J. mandshurica substrate. Yields of fruiting body show the highest to 163 g/850 mL of medium which are addition 10% of J. mandshurica sawdust, and diameter and thick of pileus were the highest, too. The L value of pileus and stipes were increased by increase of J. mandshurica substrate, but there was no significant difference in the a-value and the b-value. The contents of $P_2O_5$ and $K_2O$ of fruiting body were increased at J. mandshurica substrate, but there was no significant difference in contents of CaO, MgO and $Na_2O$. The contents of Cu of fruiting body was decreased by increase of J. mandshurica substrate, but Fe, Mn and Zn were increased.

• The Korean Journal of Mycology
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• v.48 no.2
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• pp.95-102
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• 2020

This study was carried out to develop a new method of cultivation of 'Hwadam' a new variety of Lentinula edodes, to improve fruiting body productivity and reduce labor. To determine the optimum filter number for box cultivation, L. edodes were assessed for their cultural characteristics and mushroom yield. During spawn running of L. edodes in the box, the temperature gradually increased to the highest value of 25.8℃, 23.7℃, and 23.0℃ on day 18 for the four filter, two filter, and no treatment groups, respectively. Oxygen concentration displayed an opposite trend to carbon dioxide concentration, reaching the lowest value on day 21. The oxygen concentration progressively increased as more filters were used (5.4%, 8.2%, and 8.9% treatment with no, two, and four filters, respectively. Growth of L, edodes in the box resulted in the highest yield (2,228 g/7kg substrate) and biological efficiency (70.7%) using four filters. The biological efficiency of the four filter treatment was 29.4% higher compared to bag cultivation (41.3%). Further studies are necessary to verify stable productivity of fruiting bodies through repeated cultivation.

• Journal of Mushroom
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• v.14 no.3
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• pp.90-93
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• 2016

'Mantari' is a new variety of oyster mushroom for bottle culture. It was bred by crossing monokaryons isolated from 'DM11732' and 'Chunchu-2ho'. The optimum temperature for the mycelial growth was $26{\sim}29^{\circ}C$ on PDA medium, and that for the primordia formation and the growth of fruiting body of 'Mantari' on sawdust media was $18^{\circ}C$ and $16^{\circ}C$. It took 32 days to finish spawn running, 4 days to finish primordia formation, and 3 days to finish fruiting body growth in the bottle culture. The fruitcharacteristics: the pileus was round and gray-black in color, and the stipe was long, thin, and light gray in color. The yield per bottle was 179 g/900 ml and was 5% higher than that of the control variety (Chunchu-2ho). The physical properties of the fruit body were as follows: springiness, cohesive, gumminess, and brittleness of the stipe tissue were 96%, 76%, 160 g, and 15 kg, respectively.

• Journal of Mushroom
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• v.18 no.1
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• pp.84-90
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• 2020

Wild-type isolates of H. erinaceus were collected from different geographical areas of Korea. Nineteen isolates were cultured on mushroom substrate for producing fruiting bodies. Of these, 14 isolates formed pinheads and fruiting bodies on the substrate. The morphological and cultivation characteristics of fruiting bodies were categorized by pinheading, fruit body formation, spine types, fresh weights, and colors. Microstructures, including spines, spores, and basidia on the fruiting bodies were observed using scanning electron microscopy. The H. erinaceus isolates demonstrated different PCR polymorphisms produced by universal fungal primers (UFPs) and were classified into four groups based on their high genetic diversity.