• Journal of agricultural medicine and community health
• /
• v.15 no.1
• /
• pp.3-8
• /
• 1990

Seven specimens of salmon(Oncorhynchus keta) purchased directly, in Oct. 10. 1990, in the Taep'o port. Kang-won-do were examined for infection state of anisakid larvae and classification of extracted larvae according to morphological characters. The results were as follows. 1) From seven salmon 202 anisakid larvae were found, and mean infection number of anisakid larvae per individual salmon was 28.86. 2) From total extracted anisakid larvae 198 larvae(98%) were found in muscle. Therefore the distribution of anisakid larvae in intestine was extremely rare compared to in muscle. 3) The percentage of anisakid larvae in II, IV muscle region was 93% and from this we could surmise that most anisakid larvae in salmon did not penetrate to the very distanted muscle from intestine. 4) Three types of anisakid larvae(Anisakis Type I, Contracaecum Type B, Contracaecum Type D) were identified and, among them, Contracaecum Type B was the first recording type in Korea 5) Larvae of Contracaecum it genus were found only in intestine. Therefore it surmised that penetration neture to muscle of Contracaecum larvae was less than that of Anisakis Type I.

• Parasites, Hosts and Diseases
• /
• v.45 no.1 s.141
• /
• pp.65-68
• /
• 2007

The application of Giemsa technique to stain compressed diaphragm samples obtained from rodents experimentally infected with Trichinella spiralis is described. Diaphragm samples from rats heavily infected with 20 muscle larvae per gram of body weight(20 ML/gbw) were cut into several pieces and stained with Giemsa; on the other hand, whole diaphragms from slightly infected mice(1 ML/gbw) were also stained with Giemsa. Besides, muscle samples were also stained with Giemsa. Observation at 10 $\times$ magnification revealed that both ML and nurse cells(NC) look as bluish structures clearly contrasting with the pinkish color of the non-infected muscle fibers. NC in the diaphragms of mice could be easily observed at naked eye as blue points contrasting with the pink surrounding areas formed by the non-infected muscle fibers. Among NC observed in the diaphragms of rats infected with 20 ML/gbw, 4.4% was multiple infection. These findings were confirmed in sectioned and hematoxylin-eosin stained specimens. This data could be usefulness for a rapid diagnosis of trichinellosis in post-mortem mammals without magnification procedures.

• Biomedical Science Letters
• /
• v.12 no.2
• /
• pp.99-104
• /
• 2006

Present study was performed to investigate the host-parasite relationship of the Korean Trichinella isolate (KTI). In the experiment to observe the infectivity of KTI to several kinds of animals, the reproductive capacity index (RCT) was highest in cats, and that in mice, hamsters and rats was followe4 in descending order. However, birds, i.e. wild goose and chicken, did not infect with KTI. The number of larvae per a gram of muscle (LPG: 377) was highest in the tongue of cats experimentally infected with KTI larvae. LPG in the diaphragm, anterior leg, back, posterior leg and abdominal muscles were 313, 246, 234, 225 and 170 respectively. Muscle larvae recovered at 55 days after infection were revealed the highest infectivity (RCI: 137.2) in mice. RCI was comparatively low in the mice infected with less than 25 day-old and more than 300 day-old larvae. In the experiment to observe the susceptibility of KTI by the mouse strain, ICR (RCI: 137.2), C57BL/6 (RCI: 108.8), DBA/2 (RCI: 107.1), C3H (RCI: 98.7), BALB/c (RCI: 96.9), FVB (RCI: 96.1) and B6C3F1 (RCI: 85.3) were very susceptible. However, BDF1 (RCI: 57.7) and CBA (RCI. 57.1) were revealed the moderate susceptibility, and B6CBAF1 (RCI: 23.1) was shown the lowest. The infection sites of adults were posteriorly transferred in the small intestine of experimental mice according to the infection periods of muscle larvae. The infection characteristics of KTI observed in this study may be useful as the basic data in the advanced studies, furthermore in the study of other Trichinella isolates.

• Parasites, Hosts and Diseases
• /
• v.35 no.3
• /
• pp.211-214
• /
• 1997

Susceptibility of some vertebrates was examined to the early third-stage larvae (EL3) of Gnathostomn hispidum. The larvae collected from the Chinese loaches were infected to 4 silk carps, 3 snake heads, 3 bullfrogs. 5 mice and 9 albino rats. No worms were detected in fish. silk carps and snake heads. In 3 bullfrogs fed 30 larvae, a total of 9 EL3 was recovered in the gastrointestinal tract (8 larvae) and liver (ones). In 5 mice inferred with 50 larvae, a tolal of 37 (74.0%) advanced third-stage larvae (AdL3) was recovered from the muscle (31 larvae), liver (5 larvae) and kidney at 4 weeks after infection. In 9 albino rats iilfected with 115 larvae, a total of 40 (34.8%) AdLa was found in the muscle. The Inammalian hosts were found susceptible to the EL3 of G. hispinum from Chinese loaches.

• Journal of fish pathology
• /
• v.23 no.1
• /
• pp.123-129
• /
• 2010

The infestation status of anisakid type larvae was investigated in migrating chum salmon (Oncorhynchus keta), with different condition (captured area, sex, body portion) during 2006~2008. The mean infection number of anisakid larvae per individual female and male fish captured from Namdae river was $98{\pm}27$, $103{\pm}27$, respectively. The mean infection number of anisakid larvae per individual female and male fish captured from the coastal area of Yangyang was $63{\pm}18$ and $108{\pm}17$, respectively. The anisakid larvae were mainly found in abdominal muscles (85%) but only a little in the visceral portion. Two types of anisakid larvae (A. simplex, Contracaecum type) were identified but other anisakid larvae were not detected. To investigate the effect of storing temperature on the viability of anisakid larvae, the section of abdominal muscle were stored at different temperature (room temperature, $4^{\circ}C$, $-20^{\circ}C$, $-80^{\circ}C$). As a result, it was necessary to store at $-20^{\circ}C$ for more than 6 hrs to kill the larvae. The present results revealed that chum salmon caught in Korea are heavily infected with anisakid larvae, mainly in the abdominal muscle, and A. simplex was dominantly found in this study.

• Journal of Environmental Health Sciences
• /
• v.26 no.2
• /
• pp.67-69
• /
• 2000

Yellow tails (Seriola quinqueradiata) were purchased from the Jakalchi fish market located in Busna and examined for anisakid larvae. The collected larvae were classified by their morphologicaltypes. Of the 16 yellow tails exmained, seven (43.8%) were infected with the larvae. Among 90 larvae, 81 were collected from the omentum and 9 from the muscle. Anisakids were classified into five larval types, anisakis type I (56 larvae in number, 62.2%), Contracaecum type A (8, 8.9%) and type D (7, 7.8%) and Contracaecum type C (19, 21.1%). The present study revealed that Anisakis type I was the most common among the five larval types in the yellow tails caught in the South sea of Korea peninsula.

• Parasites, Hosts and Diseases
• /
• v.41 no.2
• /
• pp.125-127
• /
• 2003

Muscle larvae of Trichinella isolates from two outbreaks in Korea were analyzed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiple-PCR. All of the muscle larvae showed a band similar to that of T. spiralis larvae of the reference strain. The two Korean Trichinella isolates (isolate code ISS623 and ISS1078) might be classifiable to Trichinella spiralis.

• Parasites, Hosts and Diseases
• /
• v.30 no.3
• /
• pp.157-162
• /
• 1992

Although the sea eel (Astroconger myriaster) is suspected as one of the most important fish host for human anisakiasis in Korea, no report has been made on the infection status of the sea eel with anisakid larvae. In the present study, 26 sea eels (Astroconger myriaster) were purchased from the Noryangjin 6sh market in Seoul, and anisakid larvae were collected from their viscera, muscle, head and skin. The collected larvae were classified by their morphological types. A total of 1,351 anisakid larvae were collected from 15 of 26 fish examined. Among them, 1,269 were recovered from the viscera, 66 from the muscle, and 16 from the head and skin. Morphologically, most of the anisakids were classified into 6 known larval types, Anisakis type I (564 larvae) of Berland(1961) , Contracaecum type A(409) and type D(5) of Koyama et at. (1969), Contracaecum type C'(83) and type D'(117) of Chai et at. (1986), and Contracaecum type V(1) of Yamaguti (1935). Remaining 172 specimens were new in the available literature, hence, designated as Centracaecum type A'(new type). The present results revealed that the sea eels caught in the Korean waters are heavily infected with anisakid larvae, not only in their viscera but also in the muscle, and Anisakis type I was the most common among the 7 larval types.

• Parasites, Hosts and Diseases
• /
• v.38 no.2
• /
• pp.111-115
• /
• 2000

Three cases of human infection by Trichinella spiralis were first confirmed by detecting encysted larvae in the biopsied muscle in December 1997, in Korea. The patients were one 35-and two 39-year-old males residing in Kochang-gun, Kyongsangnam-do. They had a common past history of eating raw liver, spleen, blood and muscle of a badger, Meles meles melanogenvs, and complained of high fever, facial and periorbital edema, and myalgia. Hematologic and biochemical examinations revealed leukocytosis and eosinophilia, and highly elevated levels of GOT, GPT, LDH and CPK. In the gastrocnemius muscle of a patient, roundly coiled nematode larvae were detected. The larvae measured 0.775-1.050 (av 0.908) mm in length, and 0.026-0.042 (av. 0.035) mm in maximum width. The specific IgG antibody levels in three patients' sera were significantly higher when compared with those of normal controls. The patients were treated with flubendazole and albendazole for 15-30 days, and discharged at 13-34 days post-admission. From the above findings, it was confirmed that T. spiralis is present in Korea, and the badger plays a role of as the natural host.

• Journal of Environmental Health Sciences
• /
• v.23 no.1
• /
• pp.14-17
• /
• 1997

A total of 93 specimens of Astroconger myriaster were purchased from the Chungmu fishery market at the south sea area during the period from October 1 to November 25, 1996. Specimens were examined for the infection status with larvae anisakid. Anisakid larvae were collected from viscera, muscle, omenturn and liver. Eight hundred four larval anisakids sorted from 93 specimens of A. myriaster ranged from 9.0 to 26.5mm in their body length. They were classified based on morphological and morphometric observations as follows Anisakis type 1 larvae (465 larvae, 57.8%: positive rate), Contracaecum type D(129, 16.0%), Contracaecum type A(90, 11.2%), Anisakis type 11(60, 7.5%), Phapidascaris(3, 0.4%) and unknown type(57, 7.1%).