• Title/Summary/Keyword: multiplication concept

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Studies on Host-Virus Interaction of Poxviruses 1. Cytochemical, Autoradiographic and Immunocytological Analysis in Cowpox Virus-FL Cell System (Poxvirus 감염(感染)에 있어서의 Virus-숙주세포(宿主細胞)의 상호관계(相互關係) 1. Cowpox Virus-FL 세포계(細胞系)의 세포화학적(細胞化學的) Autoradiography 및 세포면역학적해석(細胞免疫學的解析))

  • Kim, Uh Ho
    • Korean Journal of Veterinary Research
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    • v.15 no.1
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    • pp.57-67
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    • 1975
  • The poxvirus group is considered to be a typical cytoplasmic inclusion forming virus. Every poxvirus has been reported to produce only one kind of inclusion in the infected tissues. A vague concept that inclusions of poxviruses are eosinophilic or acidophilic has prevailed. Although many papers and theories about the nature of the inclusion have been presented, most of them are not quite convincing on the point of the relations with virus multiplication, and an analysis of papers published showed that there seem to be many discrepancies in the descriptions of the nature of the poxvirus inclusions. Comparative studies on host-virus interaction with cowpox, orf, swinepox and fowlpox viruses which selected from each Group (I-IV) of poxviruses were performed from the morphological and virological standpoints. At first, in cowpox virus-FL cell system, as a comparative model, cytoplasmic inclusion, nucleic acid metabolism by autoradiography and detection of viral antigen by immunofluorescence were studied and obtained the results as follows: 1. The focus-like cytopathic effect (CPE) at early stage developed to entire culture at terminal stage of infection, and also the developing status of CPE was correlated to viral doses for inoculation. Two kinds of cytoplasmic inclusions which named A and B type were easily observed by Giemsa, hematoxylin-eosin (H & E) and May-Greenwald Giemsa (MGG) stainings in the infected cells. The B type inclusions were formed at early stage of infection and the A type inclusions were produced subsequently the B type formation. The B type which common type inclusion in poxviruses was a small compact or aggregate at early stage and developed to a large diffuse body at terminal stage of infection. On the other hand, the A type inclusion which depend upon the kind of virus was appeared as round and discrete shape, and its size and number was increased gradually during the culture period. It was characteristic to form distinct halos around the both types of inclusions in acid fixed, H & E stained preparations of infected cultures. The B type inclusion was always positive in Feulgen reaction and showed as DNA containing body but the A type inclusion was not. 2. In the relationship between inclusion and DNA metabolism of infected cells by the qualitative autoradiography using 3H-thymidine, the appearance of silver grains was coincided with B type inclusion but not with A type inclusion. This showed that the DNA synthesis was proceeded in all B type inclusions except those in the terminal stage with a diffuse form. This suggested that the B type inclusions are only sites of DNA synthesis and this was proceeded after the cell infection independently. The activity of DNA synthesis of the inclusions was nearly the same as that of the nucleic of normal cells and non-inclusion bearing cells. and non-inclusion bearing cells. Regardless of the size of the degree of DNA synthesis of the B type inclusion, inclusion bearing cells all showed remarkable suppression of nuclear DNA synthesis. 3. By the direct fluorescent antibody technique viral antigen in infected cells was detected. The B type inclusions have been proved to contain a great deal of viral antigen, whereas the basic substance of A type inclusion did not show antigenicity except the round edge. It was suggested that the round edge fluorescence might be caused by the glare of cytoplasmic viral antigen which pushed out and concentrated by the A type inclusion development. 4. Hemorrhagic red pock formations on chorioallantoic membrane of embryonated chicken egg had proved the characteristic of used viral strain. 5. By the above studies on the nature of two types of inclusions and the role they play in virus multiplication, it was concluded that the B type inclusion must be the site of the synthesis of viral DNA and protein as well as the site of the virus.

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The Microbiological Assessment of a University Foodservice Establishment, and Hazard Analysis for Quality Control of Fried Fish Cake Soup Preparation (대학 급식시설의 위생 실태조사 및 품질관리를 위한 연구 - 제 1 보 : 오뎅국을 중심으로 -)

  • Rew, Kyung;Kim, Jeong-Mi;Kwak, Tong-Kyung
    • Journal of Nutrition and Health
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    • v.18 no.4
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    • pp.283-292
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    • 1985
  • A sanitary quality of a university foodservice establishment was assessed in terms of time and temperature, pH and Aw, and microbiological evaluation. Critical control points during various phases in product flow of fried fish cake soup were identified using hazard analysis concept. The results are summarized as follows : 1) Time and temperature data indicated that phases of holding ingredients at room temperature after cooking, and hot - holding of soup before assembly were critical. 2) pH and Aw values were in favorable for microbial growth. 3) Microbiological data indicated that microbial quality of ingredients was in poor condition. A holding practice of cooked ingredients at room temperature might provide the chance for microbial contamination and multiplication. Hot - holding time and temperature of soup should be strictly controlled to assure the food safety, since the microbial quality of serving food can be directly influenced by the hot - holding temperature of soup. 4) Microbiological test results for food containers, equipments, working surfaces, and supplies indicated that sanitary conditions of earthenware, gloves, wiping cloths and cutting board should be improved promptly. 5) Critical control points identified were : ingredients, pre - preparation, post - preparation, and holding before assembly and service. 6) Guidelines for the effective quality control program for this operation were suggested.

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Development of Korean Chemical Ranking and Scoring System (CRS-Korea) and its Application to Prioritizing National Toxic Chemicals (화학물질 우선순위 선정 기법(CRS-Korea)의 개발과 적용)

  • Park Hoa-Sung;Kim Ye-shin;Lee Dong Soo;Shin Yong-seung;Choi Seung-pil;Park Seong-eun;Kim Myung-hyun;Yang Ji-yeon;Shin Dong-chun
    • Environmental Analysis Health and Toxicology
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    • v.20 no.2 s.49
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    • pp.109-121
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    • 2005
  • A chemical ranking and scoring (CRS-Korea) system was developed and proposed to use as the first step to prioritize the toxic chemicals for the purpose of monitoring and detailed risk assessment that might follow as necessary. The CRS-Korea system takes a basic concept of risk assessment (both human health risk and ecological risk) in that risk score is determined by the product of toxicity score and exposure score. Included in the toxicity category are acute toxicity, chronic/sub -chronic toxicity, carcinogenicity, and other toxicity. The exposure category consists of quantity released to the environment, bioconcentration, and persistence. A consistent scheme and a comprehensive chemical data base are offered in the CRS-Korea system to calculate a score for the each component in the two categories by using specific physicochemical, fate, and toxic properties and the quantity of the chemical used. The toxicity score is obtained by adding up all the individual scores for the components in the toxicity category. The exposure score is determined by multiplication of the score of the quantity released with the sum of persistent score and bioconcentration score. Equal weight is given to the toxicity score and the exposure score. As the CRS-Korea system was applied to identify 50 national priority chemicals, it was found that significant data gap exists on toxicity and fate properties and that the uncertainty associated with estimating the quantify released to the environment is notably high. The proposed CRS system is only a screening tool in the first step toward the priority setting and should be used with expert judgement and other considerations necessary.

Quantitative Methodology to Assess Cyber Security Risks of SCADA system in Electric Power Industry (전력 SCADA 시스템의 사이버 보안 위험 평가를 위한 정량적 방법론에 관한 연구)

  • Kang, Dong-Joo;Lee, Jong-Joo;Lee, Young;Lee, Im-Sop;Kim, Huy-Kang
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.23 no.3
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    • pp.445-457
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    • 2013
  • This paper is about the study to build a quantitative methodology to assess cyber threats and vulnerabilities on control systems. The SCADA system in power industry is one of the most representative and biggest control systems. The SCADA system was originally a local system but it has been extended to wide area as both ICT and power system technologies evolve. Smart Grid is a concept to integrate energy and IT systems, and therefore the existing cyber threats might be infectious to the power system in the integration process. Power system is operated on a real time basis and this could make the power system more vulnerable to the cyber threats. It is a unique characteristic of power systems different from ICT systems. For example, availability is the most critical factor while confidentiality is the one from the CIA triad of IT security. In this context, it is needed to reflect the different characteristics to assess cyber security risks in power systems. Generally, the risk(R) is defined as the multiplication of threat(T), vulnerability(V), and asset(A). This formula is also used for the quantification of the risk, and a conceptual methodology is proposed for the objective in this study.

An Adaptive Decision-Feedback Equalizer Architecture using RB Complex-Number Filter and chip-set design (RB 복소수 필터를 이용한 적응 결정귀환 등화기 구조 및 칩셋 설계)

  • Kim, Ho Ha;An, Byeong Gyu;Sin, Gyeong Uk
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.24 no.12A
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    • pp.2015-2024
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    • 1999
  • Presented in this paper are a new complex-umber filter architecture, which is suitable for an efficient implementation of baseband signal processing of digital communication systems, and a chip-set design of adaptive decision-feedback equalizer (ADFE) employing the proposed structure. The basic concept behind the approach proposed in this paper is to apply redundant binary (RB) arithmetic instead of conventional 2’s complement arithmetic in order to achieve an efficient realization of complex-number multiplication and accumulation. With the proposed way, an N-tap complex-number filter can be realized using 2N RB multipliers and 2N-2 RB adders, and each filter tap has its critical delay of $T_{m.RB}+T_{a.RB}$ (where $T_{m.RB}, T_{a.RB}$are delays of a RB multiplier and a RB adder, respectively), making the filter structure simple, as well as resulting in enhanced speed by means of reduced arithmetic operations. To demonstrate the proposed idea, a prototype ADFE chip-set, FFEM (Feed-Forward Equalizer Module) and DFEM (Decision-Feedback Equalizer Module) that can be cascaded to implement longer filter taps, has been designed. Each module is composed of two complex-number filter taps with their LMS coefficient update circuits, and contains about 26,000 gates. The chip-set was modeled and verified using COSSAP and VHDL, and synthesized using 0.8- μm SOG (Sea-Of-Gate) cell library.

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Artistic Achievements of Locomotive Overlap -Focusing on (1968) by Norman McLaren- (동체중첩의 예술적 성취 -노먼 맥라렌(Norman McLaren)의 <빠 드 되(Pas de deux)>(1968)를 중심으로-)

  • Moon, Jae-Cheol;Kim, Si-Hun
    • Cartoon and Animation Studies
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    • s.49
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    • pp.87-112
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    • 2017
  • Overlap what images are laid on top of each other performs various purposes of artistic expressions. Especially, overlap to describe motion has been placed on lots of art fields analysing and connecting each part of motion. This kind of overlap is called as 'Locomotive overlap' and it is defined as 'Technique for multiplication to arrange simultaneously motions of single object on one screen'. Locomotive overlap was started from fine art and photography, so this static kind of it is classified as 'Static locomotive overlap'. Afterwards, film and animation succeeded static locomotion overlap and realized real dynamic locomotive overlap with moving images, so this dynamic kind of it is classified as 'Dynamic locomotive overlap'. Most of all, by Norman McLaren accomplished its own artistic value and aesthetics in locomotive overlap has been placed in the center of the history of locomotive overlap, so to analyze this work and to research before and after it will confirm context and artistic achievements of locomotive overlap. Nowdays locomotive overlap is extending its technology and dimension more and more on the strength of development of digital technology. Some of works using digital technology show new possibilities of locomotive overlap by reinterpreting original media and concept of . Ultimately, this research for locomotive overlap focusing on the analysis of confirms artistic achievements of locomotive overlap and suggests to contemplate the meaning and new possibilities of it.