• Journal of Ginseng Research
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• v.30 no.3
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• pp.132-136
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• 2006

This study was carried out to study the effect of emergence type of multiple stem and main bud on the quality of fresh and red ginseng in Korean ginseng. To achieve the aim of this study, characteristics of roots and quality factors were investigated. Single stem plants were 62.9% of the total samples, and the remainder(37.1%) were multiple stem plants. The number of stems affected considerably on root weight. Root weight of triple stem type with triple main bud was the largest among the types. As the number of stem per plant increased, the root quality became worse. In both single and multiple stem, the more the number of main buds was, the lower the quality grade was. Yield of the red ginseng was about 30%, showing little difference between single stem and multiple stem plant. As the number of stem increased, yield of Bonsam decreased. The quality grade of red ginseng of single stem was better than that of multiple stem. Multiple stem plant produced relatively more Yangsam and Japsam. As the number of main bud increased, the quality grade of ginseng decreased.

• Journal of Korean Society of Forest Science
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• v.76 no.4
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• pp.361-369
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• 1987

The idea of multiple-use of forest land is tile one field of economics to improve the efficiency of forest land, and is the famous management technique widely used in the developed forestry country. This paper introduces the STEM and the constraint method, which is one kind of mathematical programming techniques used for multiple forest Land use, and discusses the differences between these two methods by using the hypothetical data.

• Biomolecules & Therapeutics
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• v.27 no.1
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• pp.15-24
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• 2019

Neural stem cells (NSCs) can proliferate and differentiate into multiple cell types that constitute the nervous system. NSCs can be derived from developing fetuses, embryonic stem cells, or induced pluripotent stem cells. NSCs provide a good platform to screen drugs for neurodegenerative diseases and also have potential applications in regenerative medicine. Natural products have long been used as compounds to develop new drugs. In this review, natural products that control NSC fate and induce their differentiation into neurons or glia are discussed. These phytochemicals enable promising advances to be made in the treatment of neurodegenerative diseases.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.131-135
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• 1995

Physiologically modified stem nodes derived from ex vitro and in vivo explants of hybrid aspen (Populus alba L.X P.grandidentata Michx. 'Crandon') were tested for their multiple shoot regeneration capacity using a broad spectrum dosage of cytokinins. Ex vitro derived stem nodes with excised axillary buds at the time of culture produced 11 to 13 multiple shoots on 20 to 30 $\mu$M zeatin containing Woody Plant Medium (WPM) after 6 weeks. Excision of axillary bud sprouts after 2 weeks of culture and culture of the remaining stem nodes on WPM with 1.0 to 2.0 $\mu$M BA or 10 to 30 $\mu$M zeatin produced 13 to 15 and 7 to 8 shoots per explant, respectively, Multiple tiny shoots were produced when in vivo derived stem nodes (on which all leaves were removed) were cultured on WPM with 30 to 50 $\mu$M 2iP or 20 to 50 $\mu$M zeatin. The greatest number of multiple tiny shoot proliferation (32 to 50 shoots per explant) were obtained when the explants were cultured on media containing 20 $\mu$M zeatin. Successful transplanting of these multiple shoots into the greenhouse and/or nursery was achieved.

• Medical Lasers
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• v.9 no.2
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• pp.134-141
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• 2020

The use of stem cell therapy to treat various diseases has become a promising approach. The ability of stem cells to self-renew and differentiate can contribute significantly to the success of regenerative medical treatments. In line with these expectations, there is a great need for an efficient research methodology to differentiate stem cells into their specific targets. Photobiomodulation (PBM), formerly known as low-level laser therapy (LLLT), is a relatively non-invasive technique that has a therapeutic effect on damaged tissue or cells. Recent advances in adapting PBM to stem cell therapy showed that stem cells and progenitor cells respond favorably to light. PBM stimulates different types of stem cells to enhance their migration, proliferation, and differentiation in vitro and in vivo. This review summarizes the effects of PBM on targeted differentiation across multiple stem cell lineages. The analytical expertise gained can help better understand the current state and the latest findings in PBM and stem cell therapy.

• Korean Journal of Otorhinolaryngology-Head and Neck Surgery
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• v.56 no.12
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• pp.749-753
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• 2013

Basically stem cells have characteristics of multi-potency, differentiation into multiple tissue types, and self-renew through proliferation. Recent advances in stem cell biology can make identifying the stem-cell like cells in various mammalian tissues. Stem cells in various tissues can restore damaged tissue. Stem cells from the adult nervous system proliferate to form clonal floating colonies called spheres in vitro, and recent studies have demonstrated sphere formation by cells in the tympanic membrane, vestibular system, spiral ganglion, and partly in the organ of Corti. The presence of stem cells in the ear raises the possibilities for the regeneration of the tympanic membrane & inner ear hair cells & neurons. But the gradual loss of stem cells postnatally in the organ of Corti may correlate with the loss of regenerative capacity and limited hearing restoration. Future strategies using endogenous stem cells in the ear can be the another treatment modality for the patients with intractable inner ear diseases.

• Biomolecules & Therapeutics
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• v.26 no.4
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• pp.380-388
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• 2018

Neural stem cells (NSCs) have the ability to self-renew and differentiate into multiple nervous system cell types. During embryonic development, the concentrations of soluble biological molecules have a critical role in controlling cell proliferation, migration, differentiation and apoptosis. In an effort to find optimal culture conditions for the generation of desired cell types in vitro, we used a microfluidic chip-generated growth factor gradient system. In the current study, NSCs in the microfluidic device remained healthy during the entire period of cell culture, and proliferated and differentiated in response to the concentration gradient of growth factors (epithermal growth factor and basic fibroblast growth factor). We also showed that overexpression of ASCL1 in NSCs increased neuronal differentiation depending on the concentration gradient of growth factors generated in the microfluidic gradient chip. The microfluidic system allowed us to study concentration-dependent effects of growth factors within a single device, while a traditional system requires multiple independent cultures using fixed growth factor concentrations. Our study suggests that the microfluidic gradient-generating chip is a powerful tool for determining the optimal culture conditions.

• IMMUNE NETWORK
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• v.13 no.3
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• pp.107-110
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• 2013

Graft-versus-host disease (GVHD) is a common complication of allogeneic stem cell transplantation (allo-SCT). However, a similar syndrome has been reported in autologous stem cell transplantation (ASCT) as well. The target organs of GVHD in ASCT are the skin, liver and gastrointestinal (GI) tract, which are consistent with those in allo-SCT. Histologic findings from the skin and the mucosa of the GI tract also show similar features. Here we describe a case of autologous GVHD involving the skin of a patient who underwent ASCT for multiple myeloma. In this patient, the response to a total prednisone dose of 0.5 mg/kg/day was unsatisfactory, and the patient required more intensive and prolonged immunosuppressive therapy with slow tapering.

• Journal of Forest and Environmental Science
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• v.39 no.2
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• pp.81-95
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• 2023

Due to the multiple ecosystem benefits that iconic large, old growth trees provide, forest managers are applying thinning treatments around these legacy trees to improve their vigor and reduce mortality, especially in the face of climate change and other forest health threats. One objectives of this study was to analyze sub-hourly stem fluctuations of legacy ponderosa (Pinus ponderosa Dougl. Ex P. & C. Laws) and sugar pines (Pinus lambertiana Dougl.) in the mixed-conifer forests of the Sierra Nevada in multiple different radius thinning treatments to assess the short-term effects of these treatments. Thinning treatments applied were: R30C0 (9.1 m radius), R30C2 (9.1 m radius leaving 2 competitors), and RD1.2 (radius equaling DBH multiplied by 1 ft/in multiplied by 1.25). The other objective was to assess climatic drivers of hourly stem fluctuations. Using the dendrometeR package, we gathered daily statistics (i.e. daily amplitude) of the stem fluctuations, as well as stem cycle statistics such as duration and magnitude of contraction, expansion, and stem radial increment. We then performed correlation analyses to assess the climatic drivers of stem fluctuations and to determine which radial thinning treatment was most effective at improving growth. We found an important role that mean solar radiation, air temperature, and relative humidity play in stem variations of both species. One of the main findings from a management perspective was that the RD1.2 treatment group allowed both species to contract less on warmer and higher solar radiation days. Furthermore, sugar pine put on more stem radial increment on higher solar radiation days. These findings suggest that the extended radius RD1.2 thinning treatment may be the most effective at releasing legacy sugar and ponderosa pine trees compared to the other forest management treatments applied.

• BMB Reports
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• v.48 no.4
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• pp.193-199
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• 2015

Degenerative retinal diseases affect millions of people worldwide, which can lead to the loss of vision. However, therapeutic approaches that can reverse this process are limited. Recent efforts have allowed the possibility of the stem cell-based regeneration of retinal cells and repair of injured retinal tissues. Although the direct differentiation of pluripotent stem cells into terminally differentiated photoreceptor cells comprises one approach, a series of studies revealed the intrinsic regenerative potential of the retina using endogenous retinal stem cells. Muller glial cells, ciliary pigment epithelial cells, and retinal pigment epithelial cells are candidates for such retinal stem cells that can differentiate into multiple types of retinal cells and be integrated into injured or developing retina. In this review, we explore our current understanding of the cellular identity of these candidate retinal stem cells and their therapeutic potential for cell therapy against degenerative retinal diseases. [BMB Reports 2015; 48(4): 193-199]