• Asian Journal for Public Opinion Research
• /
• 제9권2호
• /
• pp.188-211
• /
• 2021

Decreasing response rates to traditional survey methods, like face-to-face and telephone interviews, have led survey practitioners around the world to seek new ways of conducting surveys in recent years." The COVID-19 pandemic exacerbated this problem because it made conducting face-to-face interviews even more difficult than before. For example, it made conducting face-to-face surveys infeasible in 2020 in South Korea, and so the Korean Academic Multimode Open Survey (KAMOS) was unable to conduct a planned face-to-face survey to recruit new panel members. The entire 8,514-member panel, established via two-stage probability-based sampling from 2016 to 2019, was invited to take three online/telephone surveys in 2020. Of these panel members, 1,352 responded to at least one survey in 2020. To test to what extent the panel remained representative of the adult South Korean population, we compared the two groups of panel members: those who responded to at least one survey in 2020 and those who did not. After weighting both groups on the basis of age, sex, and geographical area, we analyzed their responses to some of the questions that were asked during multiple rounds of the face-to-face panel-recruiting interviews. Using Cohen's d for survey items that could be analyzed numerically and Cramér's V for categorical items, we were able to conclude that the respondents to the 2020 surveys were equivalent to the non-respondents in terms of both demographics and in the answers they originally gave to substantive questions on a variety of topics related to social science or public opinion research, including questions about quality of life, societal issue, and politics (Cohen's d items <0.2, 95% CI; Cramér's V items <0.1, 95% CI). This analysis may provide a model for others who wish to test the continued representativeness of their panel or who would like to use a different survey mode or change some other aspect of their methodology and test whether it is equivalent to their former methodology. Our success in building a panel that retained its representativeness may be useful to those in other countries where face-to-face surveys had previously been the norm but are becoming increasingly difficult to conduct.

• 한국미생물·생명공학회지
• /
• 제49권2호
• /
• pp.174-180
• /
• 2021

Chalcones exhibit multiple biological activities. Various studies have attempted to modify the structure of chalcones with a special focus on the addition of substituents to the benzene rings. However, these chemical modifications did not improve the water solubility and bioavailability of chalcones. Glycosylation can markedly affect the physical and chemical properties of hydrophobic compounds. Here, we evaluated the ability of a highly promiscuous glycosyltransferase (GT) BsGT1 from Bacillus subtilis ATCC 6633 to biosynthesize chalcone glucosides. Purified BsGT1 catalyzed the conversion of 4'-hydroxychalcone (compound 1), 4'-hydroxy-4-methylchalcone (compound 2), and 4-hydroxy-4'-methoxychalcone (compound 3), into chalcone 4'-O-β-D-glucoside (compound 1a), 4-methylchalcone 4'-O-β-D-glucoside (compound 2a), and 4'-methoxychalcone 4-O-β-D-glucoside (compound 3a), respectively. To avoid the addition of expensive uridine diphosphate glucose (UDP-Glc), a whole-cell biotransformation system was employed to provide a natural intracellular environment for in situ co-factor regeneration. The yields of compounds 1a, 2a, and 3a were as high as 90.38%, 100% and 74.79%, respectively. The successful co-expression of BsGT1 with phosphoglucomutase (PGM) and UDP-Glc pyrophosphorylase (GalU), which are involved in the biosynthetic pathway of UDP-Glc, further improved the conversion rates of chalcones (the yields of compounds 1a and 3a increased by approximately 10%). In conclusion, we demonstrated an effective whole-cell biocatalytic system for the enzymatic biosynthesis of chalcone β-D-glucoside derivatives.

• 한국환경보건학회지
• /
• 제48권4호
• /
• pp.216-226
• /
• 2022

Background: The amount of smoking in adolescence increases with a younger age of smoking initiation and affects physical health. To establish and evaluate smoking-related policies, it is important to determine actual smoking status. Validation of self-reported questionnaires can identify the accuracy of the questionnaire data reflecting smoking status. Objectives: The purpose of this study was to evaluate the validity of self-reported smoking status and identify factors affecting the accuracy of self-reported smoking in South Korean adolescents. Methods: This study investigated the consistency between cotinine concentrations and self-reported questionnaire data through the analysis of urine samples collected from 922 adolescents aged 13~18 among the participants of Cycle 3 of the Korean National Environmental Health Survey. Smoking status was classified using the cotinine cut-off point of 39.85 ㎍/L in adolescents, and factors affecting the accuracy were analyzed through multiple logistic regression analysis. Results: The smoking rates according to the self-reported questionnaire and cut-off point-based cotinine concentrations among adolescents were 3.1% and 5.1%, respectively. The results found 97.1% consistency between self-reported smokers and smokers according to cotinine concentration. Factors affecting the discrepancy showed a significant relationship, including gender, secondhand smoke, and use of e-cigarettes. Conclusions: The results can be used as basic data to establish a smoking policy for adolescents through continuous monitoring and improvement of questionnaire items of factors affecting the discrepancy.

• Journal of Microbiology and Biotechnology
• /
• 제33권2호
• /
• pp.167-179
• /
• 2023

The rifampicin-resistant strain E9-302 of Edwardsiella ictaluri strain 669 (WT) was generated by continuous passage on BHI agar plates containing increasing concentrations of rifampicin. E9-302 was attenuated significantly by 119 times to zebrafish Danio rerio compared to WT in terms of the 50% lethal dose (LD₅₀). Zebrafish vaccinated with E9-302 via intraperitoneal (IP) injection at a dose of 1 × 10³ CFU/fish had relative percentage survival (RPS) rates of 85.7% when challenged with wild-type E. ictaluri via IP 14 days post-vaccination (dpv). After 14 days of primary vaccination with E9-302 via immersion (IM) at a dose of 4 × 10⁷ CFU/ml, a booster IM vaccination with E9-302 at a dose of 2 × 10⁷ CFU/ml exhibited 65.2% RPS against challenge with wild-type E. ictaluri via IP 7 days later. These results indicated that the rifampicin-resistant attenuated strain E9-302 had potential as a live vaccine against E. ictaluri infection. A previously unreported amino acid site change at position 142 of the RNA polymerase (RNAP) β subunit encoded by the gene rpoB associated with rifampicin resistance was identified. Analysis of the whole-genome sequencing results revealed multiple missense mutations in the virulence-related genes esrB and sspH2 in E9-302 compared with WT, and a 189 bp mismatch in one gene, whose coding product was highly homologous to glycosyltransferase family 39 protein. This study preliminarily explored the molecular mechanism underlying the virulence attenuation of rifampicin-resistant strain E9-302 and provided a new target for the subsequent study of the pathogenic mechanism of E. ictaluri.

• 한국임상약학회지
• /
• 제33권1호
• /
• pp.62-69
• /
• 2023

Objective: Adherence is an important component in the treatment of various diseases, and poor adherence to antidepressants in patients with major depressive disorder is common. Non-adherence can be more prevalent in elderly patients with multiple morbidity and polypharmacy, resulting in negative treatment outcomes. The purpose of this study was to analyze adherence to antidepressants in Korean elderly patients with major depressive disorder. Method: A retrospective study was conducted using the Korean National Health Insurance claims database, and the subjects of this study were patients aged 65 or older who received at least one prescription of antidepressant monotherapy for the treatment of major depressive disorder between January 1, 2020 and June 30, 2020. Adherence was measured using the proportion of days covered at 6 months after the initial antidepressant prescription date. Logistic regression analysis was used to identify factors associated with adherence. Results: A total of 416,766 patients were finally included in the study. Over half of patients were non-adherent (52.67%) to antidepressants. According to the multivariate logistic regression analysis, national health insurance or medical aid, taking selective serotonin reuptake inhibitors or selective norepinephrine reuptake inhibitors, and having comorbidities were significantly associated with greater rates of adherence in the study subjects. The highest adherence rate was observed in patients taking vortioxetine. Conclusion: There was a considerable rate of non-adherence in Korean elderly patients with major depressive disorder. Health care professionals should try to improve adherence in elderly patients with major depressive disorder.

• 한국정보통신학회논문지
• /
• 제26권12호
• /
• pp.1759-1768
• /
• 2022

본 논문은 AI Hub에서 제공하는 웰니스 대화 스크립트, 주제별 일상 대화 데이터세트와 Github에 공개된 챗봇 데이터세트를 활용하여 사용자의 발화에서 우울 관련 감정을 탐지하는 모델을 제안한다. 우울 관련 감정에는 우울감, 무기력을 비롯한 18가지 감정이 존재하며, 언어 모델에서 높은 성능을 보이는 KoBERT와 KoELECTRA 모델을 사용하여 감정 분류 작업을 수행한다. 모델별 성능 비교를 위해 우리는 데이터세트를 다양하게 구축하고, 좋은 성능을 보이는 모델에 대해 배치 크기와 학습률을 조정하면서 분류 결과를 비교한다. 더 나아가, 사람은 동시에 여러 감정을 느끼는 것을 반영하기 위해, 모델의 출력값이 특정 임계치보다 높은 레이블들을 모두 정답으로 선정함으로써, 다중 분류 작업을 수행한다. 이러한 과정을 통해 도출한 성능이 가장 좋은 모델을 Depression model이라 부르며, 이후 사용자 발화에 대해 우울 관련 감정을 분류할 때 해당 모델을 사용한다.

• 한국음향학회지
• /
• 제41권2호
• /
• pp.218-226
• /
• 2022

수중 음향 채널의 주요 특징은 다중 경로와 빠른 주파수 페이딩을 가지고 있다. 이로 인해 다중 주파수 대역을 가지는 주파수 변이 변조(Frequency Shift Keying, FSK) 신호는 다중 경로로 인한 선택적 페이딩으로 인하여 특정한 주파수에 할당된 정보 비트의 손실을 가져온다. 따라서 본 논문에서는 프리엠블 오류율 기반으로 각 중심 주파수의 이득을 조절하는 알고리즘을 제안하였다. 제안한 알고리즘은 수신되는 신호의 페이딩으로 인한 특정 주파수의 이득을 변화하면서 프리엠블 오류율이 10 % 미만인 영역의 값을 할당하여 최적의 이득 값을 추정하였다. 경북 문경의 호수에서 300 m ~ 500 m의 이동 거리를 가지는 호수 실험을 하였으며, 부호화율 1/3을 가지는 주파수 변이 변조 신호의 터보 등화 모델에서 터보 등화 반복 횟수가 증가함에 따라 제안한 알고리즘의 적용 시 모두 복호됨을 확인하였다.

• Journal of Korean Neurosurgical Society
• /
• 제66권4호
• /
• pp.382-392
• /
• 2023

Objective : The use of indocyanine green videoangiography (ICG-VA) to assess blood flow in the brain during cerebrovascular surgery has been increasing. Clinical studies on ICG-VA have predominantly focused on qualitative analysis. However, quantitative analysis numerical modelling for time profiling enables a more accurate evaluation of blood flow kinetics. In this study, we established a multiple exponential modified Gaussian (multi-EMG) model for quantitative ICG-VA to understand accurately the status of cerebral hemodynamics. Methods : We obtained clinical data of cerebral blood flow acquired the quantitative analysis ICG-VA during cerebrovascular surgery. Varied asymmetric peak functions were compared to find the most matching function form with clinical data by using a nonlinear regression algorithm. To verify the result of the nonlinear regression, the mode function was applied to various types of data. Results : The proposed multi-EMG model is well fitted to the clinical data. Because the primary parameters-growth and decay rates, and peak center and heights-of the model are characteristics of model function, they provide accurate reference values for assessing cerebral hemodynamics in various conditions. In addition, the primary parameters can be estimated on the curves with partially missed data. The accuracy of the model estimation was verified by a repeated curve fitting method using manipulation of missing data. Conclusion : The multi-EMG model can possibly serve as a universal model for cerebral hemodynamics in a comparison with other asymmetric peak functions. According to the results, the model can be helpful for clinical research assessment of cerebrovascular hemodynamics in a clinical setting.

• Journal of Hospice and Palliative Care
• /
• 제25권2호
• /
• pp.55-65
• /
• 2022

Caring for patients with cancer is highly stimulating and rewarding, attracting health professionals to the field who enjoy the challenge of managing a complex illness. Health professionals often form close bonds with their patients as they confront ongoing disease or treatment impacts, which may be associated with multiple losses involving function and/or eventual loss of life. Ongoing exposure to patient loss, along with a challenging work setting, may pose significant stress and impact health professionals' well-being. The prevalence rates of burnout and compassion fatigue (CF) are significant, yet health professionals have little knowledge on these topics. A 6-week continuing education program consisting of weekly small-group video-conferencing sessions, case-based learning, and an online community of practice was delivered to health care providers providing oncology care. Program content included personal, organization and team-related risk and protective factors associated with CF, grief models, and strategies to mitigate against CF. Content analysis was completed as part of the program evaluation. In total, 189 participants (93% nurses) completed the program, which was associated with significant improvements in confidence and knowledge of CF and strategies to support self and team resilience. Qualitative themes and vignettes from experiences with the program are presented. Key themes included knowledge gaps, a lack of support related to CF and strategies to support resilience, organization-and team-based factors that can inhibit expression about the impacts of clinical work, the health professional as a "person" in caregiving, and the role of personal variables, self-skill practices, and recommendations for education and support for self and teams.

• Journal of Nutrition and Health
• /
• 제56권3호
• /
• pp.330-347
• /
• 2023

Purpose: This study identified words closely associated with the keyword "dietary supplement" (DS) using big data in Korean social media and investigated consumer perceptions and trends related to DSs before (2019) and after the coronavirus disease 2019 (COVID-19) pandemic (2021). Methods: A total of 37,313 keywords were found for the 2019 period, and 35,336 keywords were found for the 2021 period using blogs and cafes on Daum and Naver. Results were derived by text mining, semantic networking, network visualization analysis, and sentiment analysis. Results: The DS-related keywords that frequently appeared before and after COVID-19 were "recommend", "vitamin", "health", "children", "multiple", and "lactobacillus". "Calcium", "lutein", "skin", and "immunity" also had high frequency-inverse document frequency (TF-IDF) values. These keywords imply a keen interest in DSs among Korean consumers. Big data results also reflected social phenomena related to DSs; for example, "baby" and "pregnant woman" had lower TD-IDF values after the pandemic, suggesting lower marriage and birth rates but higher values for "joint", indicating reduced physical activity. A network centered on vitamins and health care was produced by semantic network analysis in 2019. In 2021, values were highest for deficiency and need, indicating that individuals were searching for DSs after the COVID-19 pandemic due to a lack an awareness of the need for adequate nutrient intake. Before the pandemic, DSs and vitamins were associated with healthcare and life cycle-related topics, such as pregnancy, but after the COVID-19 pandemic, consumer interests changed to disease prevention and treatment. Conclusion: This study provides meaningful clues regarding consumer perceptions and trends related to DSs before and after the COVID-19 pandemic and fundamental data on the effect of the pandemic on consumer interest in dietary supplements.