• Molecules and Cells
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• v.26 no.6
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• pp.536-547
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• 2008

Anthocyanidin synthase (ANS, leucoanthocyanidin oxygenase), a 2-oxoglutarate iron-dependent oxygenase, catalyzed the penultimate step in the biosynthesis of the anthocyanin class of flavonoids, from the colorless leucoanthocyanidins to the colored anthocyanidins. The full-length cDNA and genomic DNA sequences of ANS gene (designated as GbANS) were isolated from Ginkgo biloba for the first time. The full-length cDNA of GbANS contained a 1062-bp open reading frame (ORF) encoding a 354-amino-acid protein. The genomic DNA analysis showed that GbANS gene had three exons and two introns. The deduced GbANS protein showed high identities to other plant ANSs. The conserved amino acids (H-X-D) ligating ferrous iron and residues (R-X-S) participating in 2-oxoglutarate binding were found in GbANS at the similar positions like other ANSs. Southern blot analysis indicated that GbANS belonged to a multi-gene family. The expression analysis by real-time PCR showed that GbANS expressed in a tissue-specific manner in G. biloba. GbANS was also found to be up-regulated by all of the six tested abiotic stresses, UV-B, abscisic acid, sucrose, salicylic acid, cold and ethylene, consistent with the promoter region analysis of GbANS. The recombinant protein was successfully expressed in E. coli strain with pET-28a vector. The in vitro enzyme activity assay by HPLC indicated that recombinant GbANS protein could catalyze the formation the cyanidin from leucocyanidin and conversion of dihydroquercetin to quercetin, suggesting GbANS is a bifunctional enzyme within the anthocyanidin and flavonol biosynthetic pathway.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.3
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• pp.303-310
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• 2015

The study was conducted to analyze the genetic parameters of somatic cell score (SCS) of Holstein cows, which is an important indicator to udder health. Test-day records of somatic cell counts (SCC) of 305-day lactation design from first to fifth lactations were collected on Holsteins in Korea during 2000 to 2012. Records of animals within 18 to 42 months, 30 to 54 months, 42 to 66 months, 54 to 78 months, and 66 to 90 months of age at the first, second, third, fourth and fifth parities were analyzed, respectively. Somatic cell scores were calculated, and adjusted for lactation production stages by Wilmink's function. Lactation averages of SCS ($LSCS_1$ through $LSCS_5$) were derived by further adjustments of each test-day SCS for five age groups in particular lactations. Two datasets were prepared through restrictions on number of sires/herd and dams/herd, progenies/sire, and number of parities/cow to reduce data size and attain better relationships among animals. All LSCS traits were treated as individual trait and, analyzed through multiple-trait sire models and single trait animal models via VCE 6.0 software package. Herd-year was fitted as a random effect. Age at calving was regressed as a fixed covariate. The mean LSCS of five lactations were between 3.507 and 4.322 that corresponded to a SCC range between 71,000 and 125,000 cells/mL; with coefficient of variation from 28.2% to 29.9%. Heritability estimates from sire models were within the range of 0.10 to 0.16 for all LSCS. Heritability was the highest at lactation 2 from both datasets (0.14/0.16) and lowest at lactation 5 (0.11/0.10) using sire model. Heritabilities from single trait animal model analyses were slightly higher than sire models. Genetic correlations between LSCS traits were strong (0.62 to 0.99). Very strong associations (0.96 to 0.99) were present between successive records of later lactations. Phenotypic correlations were relatively weaker (<0.55). All correlations became weaker at distant lactations. The estimated breeding values (EBVs) of LSCS traits were somewhat similar over the years for a particular lactation, but increased with lactation number increment. The lowest EBV in first lactation indicated that selection for SCS (mastitis resistance) might be better with later lactation records. It is expected that results obtained from these multi-trait lactation model analyses, being the first large scale SCS data analysis in Korea, would create a good starting step for application of advanced statistical tools for future genomic studies focusing on selection for mastitis resistance in Holsteins of Korea.

• Korean Journal of Food Science and Technology
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• v.41 no.2
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• pp.136-140
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• 2009

The verification of irradiation treatments, using dried garlic and cabbage treated at 0-20 kGy, was investigated by analyzing the photostimulated luminescence (PSL), electron spin resonance (ESR) and thermoluminescence (TL) characteristics of the samples. The PSL results showed that the photon counts/60 sec of the non-irradiated dried garlic and cabbage were 287-337, corresponding to negative, while those of the irradiated samples were 7511-54063 photon counts/ 60 sec, corresponding to positive, making it possible to discriminate the non-irradiated from the irradiated samples. In ESR analysis, the dried garlic irradiated at 20 kGy exhibited cellulose radicals, whereas the irradiated dried cabbage showed crystalline sugar-induced multi-component signals, which were not found in the non-irradiated samples. The ESR signal intensity significantly increased as the irradiation dose increase ($R^2$= 0.9369 - 0.9926). The TL glow curves of the irradiated samples appeared at a temperature interval of 150-250, which were significantly different from those of non-irradiated samples, showing a significant increase in TL signal intensity with irradiation dose ($R^2$= 0.9670 - 0.9768). To enhance the reliability of the results, the first glow curve ($TL_1$) was compared with the second glow curve ($TL_2$) obtained after a re-irradiation step at 1 kGy. The TL ratio ($TL_1/TL_2$) was in good agreement with the reported TL threshold values for both the non-irradiated (<0.1) and irradiated (> 0.1) samples.

• 한국해양학회지
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• v.22 no.2
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• pp.57-62
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• 1987

The Markov chain properties of the sea surface temperature (SST) anomalies, namely, the dependency of the monthly SST anomaly on that of the previous month, are studied based on the SST data for 28years(1957-1984) at 5 stations in the southeastern coast of Korea. Wi classified the monthly SST anomalies at each station into the low, the normal and the high state, and computed transition probabilities between SST anomalies of two successive months The standard deviation of SST anomalies at each station is used as a reference for the classification of SST anomalies into 3states. The transition probability of the normal state to remain in the same state is about 0.8. The transition probability of the high or the low states to remain in the same state is about one half. The SST anomalies have almost no probability to transit from the high (the low) state to the low (the high) state. Statistical tests show that the Markov chain properties of SST anomalies are stationary in tine and homogeneous in space. The multi-step Markov chain analysis shows that the 'memory' of the SST anomalies at the coastal stations remains about 3 months.

• Journal of Korean Home Economics Education Association
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• v.33 no.1
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• pp.151-167
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• 2021

This study reports on the development of a dietary education program for Korean young adults in single-person households. The 7th National Health and Nutrition Survey (2016-2018) was used to compare and analyze the dietary behavior of single-person households and multi-person households, and an online survey was conducted on 350 young adults (age 19-39 years) living in Seoul. According to the analysis, single-person households had higher rates of breakfast and eating out than multi-person households, and significantly lower average intake of energy and nutrients (p<0.05). In particular, in the case of single-person households, the lower the frequency of cooking at home, the higher the rate of breakfast and the higher the frequency of eating out and delivery food (p<0.05). Based on the survey, a dietary education program for young adults single-person households was developed by applying the DESIGN six-step procedure and social cognitive theory as a conceptual model. The first session consisted of the health and economic benefits of home-cooked meals, the second session of the importance of the breakfast and the effect of exercise in life, the third session of the importance of balanced nutrition and the principles of a healthy diet, the fourth session of food safety and storage, and the fifth session of social dining. Each session was composed of a combination of theoretical lectures to motivate 'more making and eating healthy home-cooked meals' and cooking practice for improving behavioral performance.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.12
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• pp.677-685
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• 2018

In construction, schedule management is the basic but important step, for the effective schedule management, the preparation of the reasonable schedule table should be prioritized. In the design stage, the optimal construction period can be selected through comparison of various conditions and construction methods considering weather conditions and site characteristics. But, At the planning phase, it is difficult to select the effective method and calculate the proper construction period by the basic data(D/B) analysis. In this paper, the construction method considering characteristics of each type and conditions of existing city was selected. For the reasonable duration calculation, we analyzed the unit schedule for RC method for open type and Shield TBM method for tunnel type. The normal project duration of construction assuming of 1,200m of extension and every 200m of ventilation was prepared by integrating each unit schedule. It was analyzed that it took 893 days for the open type and 616 days for the tunnel type. The results of this study will help to make type selection and normal project duration more easily in the planning phase. If it is linked to the design stage, it will be easy to estimate the process and construction cost.

• BMB Reports
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• v.40 no.5
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• pp.715-722
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• 2007

A new full-length cDNA encoding hyoscyamine $6\beta$-hydroxylase (designated as aah6h, GenBank Accession No. EF187826), which catalyzes the last committed step in the scopolamine biosynthetic pathway, was isolated from young roots of Anisodus acutangulus by rapid amplification of cDNA ends (RACE) for the first time. The full-length cDNA of aah6h was 1380 bp and contained a 1035 bp open reading frame (ORF) encoding a deduced protein of 344 amino acid residues. The deduced protein had an isoelectric point (pI) of 5.09 and a calculated molecular mass of about 38.7 kDa. Sequence analyses showed that AaH6H had high homology with other H6Hs isolated from some scopolamine-producing plants such as Hyoscyamus niger, Datura metel and Atropa belladonna etc. Bioinformatics analyses results indicated AaH6H belongs to 2-oxoglutarate-dependent dioxygenase superfamily. Phylogenetic tree analysis showed that AaH6H had closest relationship with H6H from A. tanguticus. Southern hybridization analysis of the genomic DNA revealed that aah6h belonged to a multi-copy gene family. Tissue expression pattern analysis firstly founded that aah6h expressed in all the tested tissues including roots, stems and leaves and indicated that aah6h was a constitutive-expression gene, which was the first reported tissue-independent h6h gene compared to other known h6h genes.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.48 no.5
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• pp.84-95
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• 2011

In this paper, we present a deblocking algorithm that removes grid and staircase noises, which are called "blocking artifacts", occurred in the compressed satellite images. Particularly, the given satellite images are compressed with equal quantization coefficients in row according to region complexity, and more complicated regions are compressed more. However, this approach has a problem that relatively less complicated regions within the same row of complicated regions have blocking artifacts. Removing these artifacts with a general deblocking algorithm can blur complex and undesired regions as well. Additionally, the general filter lacks in preserving the curved edges. Therefore, the proposed algorithm presents an adaptive filtering scheme for removing blocking artifacts while preserving the image details including curved edges using the given quantization step size and content analysis. Particularly, WLFPCA (weighted lowpass filter using principle component analysis) is employed to reduce the artifacts around edges. Experimental results showed that the proposed method outperforms SA-DCT in terms of subjective image quality.

• Journal of the Korean Society for Library and Information Science
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• v.45 no.4
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• pp.53-76
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• 2011

This study assesses research patterns and trends of library and information science(LIS) in Korea by applying bibliometric analysis. For the study, 2,400 peer-reviewed publications from 2001 to 2010(including conference proceedings) published by 159 LIS professors in Korea were analyzed by year, author, affiliation and journal. The study findings showed an increasing trend in collaboration(52.75% of total publications with single authors and 47.25% with multiple authors) among LIS professors in Korea, robust publication patterns of Korean LIS faculty(average 1.51 publications per year), and an increasing number in foreign publications(170 publications). The study results also suggested an internationalization of LIS in Korea. Specifically, the study found a higher rate of Korean LIS faculty with foreign degrees than in previous years as well as a higher publication rate of professors with international degrees. The analysis of publication patterns conducted by the study, which is a first step in our aim to establish a multi-faceted approach for assessing the impact of scholarly work, will be followed up with analysis of references and citations to evaluate the quality of publications.

• Journal of Internet Computing and Services
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• v.16 no.1
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• pp.39-46
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• 2015

This paper presents an automatic method to detect and count the cells from microorganism images based on mobile environments. Cell counting is an important process in the field of biological and pathological image analysis. In the past, cell counting is done manually, which is known as tedious and time consuming process. Moreover, the manual cell counting can lead inconsistent and imprecise results. Therefore, it is necessary to make an automatic method to detect and count cells from biological images to obtain accurate and consistent results. The proposed multi-step cell counting method automatically segments the cells from the image of cultivated microorganism and labels the cells by utilizing topological analysis of the segmented cells. To improve the accuracy of the cell counting, we adopt watershed algorithm in separating agglomerated cells from each other and morphological operation in enhancing the individual cell object from the image. The system is developed by considering the availability in mobile environments. Therefore, the cell images can be obtained by a mobile phone and the processed statistical data of microorganism can be delivered by mobile devices in ubiquitous smart space. From the experiments, by comparing the results between manual and the proposed automatic cell counting we can prove the efficiency of the developed system.