• BMB Reports
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• v.46 no.2
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• pp.65-72
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• 2013

In situ detection of RNAs is becoming increasingly important for analysis of gene expression within and between intact cells in tissues. International genomics efforts are now cataloging patterns of RNA transcription that play roles in cell function, differentiation, and disease formation, and they are demon-strating the importance of coding and noncoding RNA transcripts in these processes. However, these techniques typically provide ensemble averages of transcription across many cells. In situ hybridization-based analysis methods complement these studies by providing information about how expression levels change between cells within normal and diseased tissues, and they provide information about the localization of transcripts within cells, which is important in understanding mechanisms of gene regulation. Multi-color, single-molecule fluorescence in situ hybridization (smFISH) is particularly useful since it enables analysis of several different transcripts simultaneously. Combining smFISH with immunofluorescent protein detection provides additional information about the association between transcription level, cellular localization, and protein expression in individual cells.

• Proceedings of the Korean Information Science Society Conference
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• 1998.10c
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• pp.435-437
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• 1998

영상에 대한 영역분할은 영상에 대한 인식 시스템에서 가장 중요하고도 어려운 분야로 알려져 있다. 주로 사용되는 방법은 화소중심기법과 영역중심기법이 사용되는데, 화소중심기법은 적은 시간이 걸리는데 비해 영역분할 효과가 떨어지고, 영역중심기법은 상대적으로 양질의 영역분할 효과를 얻을 수 있지만 많은 시간이 걸린다. 본 논문에서는 영역분할에 대한 방법으로 thresholding방법을 이용한 2단계로 이루어진 영역분할 방법을 제안한다. 제안된 방법은 화소의 전역정보와 지역정보를 모두 사용하여 기존의 전역 thresholding방법에 비해 향상된 영역 분할을 수행하고, 지역정보를 이용하는 영역중심 기법에 비해 시간을 단축하는 효과를 가지고 있다. 첫 번째 단계에서는 기존에 알려진 전역 thresholding방법을 사용하여 영역분할을 하고, 두 번째 단계에서는 영상에 대해 미리 알려진 사전지식을 이용하여 영역분할이 제대로 되지 않은 영역을 구분하여 해당 영역에 대해서만 thresholding작업을 수행한다. 사용된 영상은 자궁경부 세포진 영상으로 대상이 되는 영역은 자궁경부 세포의 핵으로 제한하였다.

• Proceedings of the KIPE Conference
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• 2010.11a
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• pp.244-245
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• 2010

본 논문은 1kW급 가정용 연료전지의 계통연계 및 고효율 전력변환 시스템 개발에 관한 것이다. 본 시스템에서 구현된 전력변환장치는 일반적인 연료전지용 PCS와 마찬가지로 DC/DC 컨버터부와 DC/AC 인버터부로 구성되며, 전체 시스템의 동작은, DC/DC 컨버터부에서는 고효율화를 위하여 공진형으로 동작되며, DC/AC 인버터부에서는 출력전력 제어와 계통연계를 수행한다.

• Journal of the Korea Society of Computer and Information
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• v.6 no.3
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• pp.72-77
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• 2001

The method of connection admission control is presented by using the equations related with traffic classes for real-time connection admission control. without assuming the cell arrival processes in the multi-level structure of ATM traffic control. The equations related with traffic classes are obtained by 3 or 4 of first order inequalities from the admissible ranges of connection considering the expected total instantaneous demand to guarantee the required quality of services of each class.

• Journal of Microbiology and Biotechnology
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• v.33 no.5
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• pp.680-686
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• 2023

Aging is a complex series of multi-organ processes that occur in various organisms. As such, an in vivo study using an animal model of aging is necessary to define its exact mechanisms and identify anti-aging substances. Using Drosophila as an in vivo model system, we identified Crataegus pinnatifida extract (CPE) as a novel anti-aging substance. Regardless of sex, Drosophila treated with CPE showed a significantly increased lifespan compared to those without CPE. In this study, we also evaluated the involvement of CPE in aging-related biochemical pathways, including TOR, stem cell generation, and antioxidative effects, and found that the representative genes of each pathway were induced by CPE administration. CPE administration did not result in significant differences in fecundity, locomotion, feeding amount, or TAG level. These conclusions suggest that CPE is a good candidate as an anti-aging food substance capable of promoting a healthy lifespan.

• Proceedings of the KSR Conference
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• 2010.06a
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• pp.640-649
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• 2010

Coarse granular materials such as gravel and crushed stone have been used as an important fill materials to large soil structure of railway, road, dam and so on. Although much studies for general soil materials have been carried out domestically, the studies for coarse materials were insufficient. Particularly, it is the level in which the study for dynamic properties(Elastic modulus and damping ratio) of coarse materials, applies the foreign country literature. This is due to the lack of large equipment for element test. But large soil structures made of coarse granular materials are generally important infrastructures. Therefore, the reliable design parameters for coarse materials should be obtained for safe and economic design, construction and maintenance. Triaxial test is the laboratory test method that is capable of controlling a confining pressure and boundary condition. In this project, we made a multi-purpose large triaxial testing system. This testing system is able to test coarse granular materials with maximum particle diameter of 100mm and support both the load control and displacement control. The load cell is installed inside of triaxial cell and the axial displacement is measured locally in order to control and measure more accurately in the small strain level. The verification test of this testing system was carried out with urethane verification specimens. So, from now on the useful information for coarse granular materials are expected to suggested by performing many tests with various material and condition.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.12C
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• pp.1014-1020
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• 2008

Storage devices such as memories are widely used in various electronic products. They require high-density memory and currently the data has been stored in multi-level format, that results in high error rate. In this paper, we apply error correction schemes that are widely used in communication system to the storage devices for satisfying low bit error rate and high code rate. In A WGN channel with average BER $10^{-5}$ and $5{\times}10^{-6}$, we study error correction schemes for 4-1evel cell to achieve target code rate 0.99 and target BER $10^{-11}$ and $10^{-13}$, respectively. Since block codes may perform better than the concatenated codes for high code rate, and it is important to use less degraded inner code even when many bits are punctured. The performance of concatenated codes using general feedforward systematic convolutional codes are worse than the block code only scheme. The simulation results show that RSC codes must be used as inner codes to achieve good performance of punctured convolutional codes for high code rate.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.11a
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• pp.430-430
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• 2008

Using multi plasma enhanced chemical vapor deposition system (Multi-PECVD), p-a-Si:H deposition layer as a $p^+$ region which was annealed by laser (Q-switched fiber laser, $\lambda$ = 1064 nm) on an n-type single crystalline Si (100) plane circle wafer was prepared as new doping method for single crystalline interdigitated back contact (IBC) solar cells. As lots of earlier studies implemented, most cases dealt with the excimer (excited dimer) laserannealing or crystallization of boron with the ultraviolet wavelength range and $10^{-9}$ sec pulse duration. In this study, the Q-switched fiber laser which has higher power, longer wavelength of infrared range ($\lambda$ = 1064 nm) and longer pulse duration of $10^{-8}$ sec than excimer laser was introduced for uniformly deposited p-a-Si:H layer to be annealed and to make sheet resistance expectable as an important process for IBC solar cell $p^+$ layer on a polished n-type Si circle wafer. A $525{\mu}m$ thick n-type Si semiconductor circle wafer of (100) plane which was dipped in a buffered hydrofluoric acid solution for 30 seconds was mounted on the Multi-PECVD system for p-a-Si:H deposition layer with the ratio of $SiH_4:H_2:B_2H_6$ = 30:120:30, at $200^{\circ}C$, 50 W power, 0.2 Torr pressure for 20 minutes. 15 mm $\times$ 15 mm size laser cut samples were annealed by fiber laser with different sets of power levels and frequencies. By comparing the results of lifetime measurement and sheet resistance relation, the laser condition set of 50 mm/s of mark speed, 160 kHz of period, 21 % of power level with continuous wave mode of scanner lens showed the features of small difference of lifetime and lowering sheet resistance than before the fiber laser treatment with not much surface damages. Diode level device was made to confirm these experimental results by measuring C-V, I-V characteristics. Uniform and expectable boron doped layer can play an important role to predict the efficiency during the fabricating process of IBC solar cells.

• Journal of IKEEE
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• v.19 no.1
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• pp.1-9
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• 2015

This paper proposes the design of sub-pixel interpolation circuit for real-time multi-decoder supporting 4K-UHD video images. The proposed sub-pixel interpolation circuit supports H.264, MPEG-4, VC-1 and new video compression standard HEVC. The common part of the interpolation algorithm used in each video compression standard is shared to reduce the circuit size. An intermediate buffer is effectively used to reduce the circuit size and optimize the performance. The proposed sub-pixel interpolation circuit was synthesised by using 130nm standard cell library. The synthesized gate-level circuit consists of 122,564 gates and processes 35~86 image frames per second for 4K-UHD video at the maximum operation frequency of 200MHz. Therefore, the proposed circuit can process 4K-UHD video in real time.

• Microbiology and Biotechnology Letters
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• v.49 no.4
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• pp.587-593
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• 2021

Infertility is a key issue affecting mood and behavior in men. Microorganisms are one of the primary etiological agents that may be associated with infertility. The objective of the present study was to identify bacterial causative agents from the semen of infertile subjects and determine the effect of bacterial infection on sperm quality, as well as determine the susceptibility of these bacteria to drugs. Forty semen samples from 30 infertile patients and 10 fertile individuals were collected. The pH, volume, motility, and concentration of semen were analyzed. The samples were processed and identified by biochemical testing using API identification kits. The antibiotic susceptibility pattern was determined using the disc diffusion method. Abnormal sperm quality was observed. The mean age of the individual and their sperm morphology, concentration, progressive motility, pH level, and pus cell content were 31.9 years, 2.7%, 10.4 million/ml, 27.3%, 8.3, and 5.7, respectively. Among the tested samples, oligoasthenozoospermia was found to show the highest occurrence, at 27/30 samples, followed by teratozoospermia, at 25/30 samples, and asthenozoospermia, at 22/30 samples. Of the tested infertile patients' sperm, 19, 6, and 5 isolates were identified as Escherichia coli, Klebsiella pneumonia, and Staphylococcus epidermidis, respectively. The results also revealed multi-drug resistance in the bacteria. Compared to that shown by the other tested antibiotics, amikacin showed higher activity against all isolated bacteria. However, the bacteria exhibited maximum resistance against gentamicin, cefotaxime, levofloxacin, and ampicillin. In conclusion, leukocytospermia and bacterial infections are possibly responsible for sperm abnormalities. Multi-drug resistant bacteria were detected. Gentamicin, cefotaxime, levofloxacin and ampicillin were shown the highest resistance, while amikacin was the most effective antimicrobial agent against the isolated bacteria.