• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.49-55
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• 2022

A total of 20 mulberry varieties preserved in Korea were typed for eight polymorphic microsatellite loci. We obtained 6 to 15 alleles per locus with an average value of 10.6, per-locus observed heterozygosity ranging from 0.35 to 1.00, and per-locus polymorphic information content (PIC) ranging from 0.61 to 0.87, indicating that most loci are highly variable. Phylogenetic analysis using the eight microsatellite loci was sufficiently suitable for classifying 20 mulberry varieties preserved in Korea. A total of 160 variety-specific apomorphic alleles were obtained from eight loci discriminated 20 mulberry varieties. These variety-specific alleles from this analysis are expected to be useful for the discrimination of other mulberry varieties. Furthermore, a substantial number of homozygote loci, represented by 60 among 180 alleles in eight loci were found. These results collectively suggest that these microsatellite locus primers are potentially crucial molecular markers for the eventual classification of mulberry varieties that are preserved as hundreds in Korea.

• 한국잠사곤충학회지
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• 제2권
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• pp.73-81
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• 1962

This treatise is to analyze the chemical components in the mulberry varieties which are cultured in Korea and to estimate the nutritive values for them. Some of these varieties were developed lately in this country just by the general breeding aspect of mulberry leaf producing and silkworm raising experimentation without analysing the nutritive values of them which may be differed by various culturing condition. This work will be helpful for the improvement of mulberry varieties and the correct judgement of them. The varieties used for the analysis are as followings; A. Morus bombycis Koridz species. 1.Ichi-Hei. 2. Shimano-Uchi B. Morus alba L. species. 3. Kairyo-Nezumigaeshi. 4. Suwon-Daeyop. 5. Suwon No. 3. 6. Suwon No. 4. 7. Yongchon-Chuwoo. C. Morus Ihou (Ser.) Koidz species. 8. Ro-Soh. The specimens were eventually taken under the air and soil dry season because of the rain shortage during the sampling period and the results are found as a rather different from the normal specimen under the normal climate. Therefore, this treatise will be an important report on a special culturing condition and the nutritive values by the mulberry varieties, and still comparable because they were grown under the same condition. The individual conclusions are; 1. Yongchon-Chuwoo which was originated in this country, was found as an important variety for this country from the aspect of mulberry nutritive value and leaf producing amount, and it is believed that this is the best suitable variety for the soil of Korea. 2. Ichi-Hei occupies the better situation as well as Yongchon-Chuwoo from the nutritive criticizing even though it was originated in Japan. 3. The lately developed Suwon No.3 and No.4 are best from the leaf producing point of view, but they are not found such a good varieties from the nutritive aspect. The result may be happened as poor because they were cut before spring sprout started. 4. Ro-Soh which was normally recognized as a poor variety, was also found as a poor nutritive and leaf producing variety. 5. The crude protein and crude fat components in the mulberry leaves decreased as the leaf maturity was progressed, but the crude fiber and crude ash components increased reversively in general view. It was also found that there were some specific changing nature by the mulberry varieties and could not rule them by just one conclusion. 6. During the air and soil dry season, the ash component increases but the hydrocarbon and moisture contents decrease considerably, and which resulted to the increase of mulberry hardness ratio and the decrease of sugar-protein ratio, eventually it causes the decrease of the nutritive value. It was also found that Ichi-Hei, Ro-Soh, and Suwon No. 3 had a strong recovering nature to their normal nutritive condition after raining. 7. Mulberry is of course a rich calorie diet, and is calculated as 50 to 60 Cal. per gram.

• 한국축산식품학회지
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• 제30권4호
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• pp.649-654
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• 2010

In order to develop new functional yogurts using mulberries and mulberry leaves, which were cultivated in Hwasun-gun, Jeonnam Province, Korea, the nutritional compositions, fermentation conditions, sensory properties, and storage stabilities of the yogurts were analyzed. The mulberry powder yogurt contained 87.96% moisture, 3.21% carbohydrate, 4.52% protein, 3.63% lipid, and 0.68% ash, and the mulberry leaf yogurt contained 86.36% moisture, 4.13% carbohydrate, 4.87% protein, 3.79% lipid, and 0.85% ash. A yogurt base was fermented for 13 h with 0.01% ABT-5 starter inoculum at $40^{\circ}C$. To prepare the mulberry jam and mulberry leaf yogurts, a variety of mulberry jam and mulberry leaf samples were added to the yogurt base. The sensory evaluation results of the yogurts containing the mulberry jam and mulberry leaves indicated that a product made with 15% mulberry jam was more strongly preferred than other samples. When the mulberry jam and mulberry leaf yogurts were stored at $4^{\circ}C$ for 15 d, there were no significant changes in pH, titratable acidity, or viable cell numbers of lactic acid bacteria and Bifidobacterium bifidum.

• 한국약용작물학회지
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• 제28권6호
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• pp.435-444
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• 2020

Background: As the Positive List System (PLS) is implemented in broad application to agricultural products, attention to the correct use of pesticides is also needed in the production of mulberry leaves and fruit. In this regard, three types of pesticides against mulberry popcorn disease were applied 2 - 3 times both in the field and greenhouses to prepare safety standards. Residual pesticide analysis was conducted on mulberry fruits and leaves. Methods and results: Three pesticieds, thiophanate-methyl, thiophanate-methyl·triflumizole and fluopyram registered as PLS pesticides for mulberry popcorn disease, were sprayed in the Wanju and in Buan regions, after which residual pesticide analysis was conducted using high-performance liquid chromatography (HPLC). As three pesticieds were either undetected or below the permissible level in mulberry fruit, demonstrating that they were suitable for safe spraying. However, 5.6 mg/kg of thiophanate-methyl was detected in the greenhouse after three application, which was slightly above maximum residue limit (MRL). Furthermore the level of thiophanate-methyl·triflumizole was higher than 0.2 mg/kg ("Gwasang No. 2" variety, spraying twice) or similar to 0.09 mg/kg ("Daesim" variety, spraying thrice) the permissible level (0.1T mg/kg) as the thiophanate-methyl was detected in mulberry leaves in the greenhouse. Conclusions: The spraying frequency for controlling mulberry popcorn disease in greenhouses should be limited to two times or less, especially when mulberry leaves are treated with thiophanate-methyl·triflumizole careful consideration is required if the leaves are to be used as food materials.

• 한국잠사곤충학회지
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• 제25권1호
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• pp.21-26
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• 1983

全原一號 品種은 1920年代 後半에 全北 原蠶種製造所에서 選拔하였으며, 1970年代 中盤까지 湖南一部 地方에 栽培되였었다. 本 品種의 正確한 特性 究明을 위하여 1977年에 水原과 全州에 改良뽕을 對照品種으로 全原一號 品種을 植栽供試하고 1979年부터 1982年가지 4個年間 栽培試驗을 實施하여 究明된 特性은 다음과 같다. 1) 全原 1號는 白桑系에 屬하는 中生種이다. 2) 枝條는 展開性, 條數型으로 伸張力이 弱하다. 3) 枝條의 枯損長比率은 改良뽕보다 약간 높으나 큰차 없었으며, 뽕나무 오갈병저항성과 耐倒伏性은 改良뽕보다 弱하다. 4) 年間改量은 正葉量으로 10a當 1,474kg으로서 改良보다 14% 적었다.

• 한국잠사곤충학회지
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• 제10권
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• pp.27-33
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• 1969

상전에 있어서 각 부위에 따르는 수량의 변이를 알고저 시험을 행하여 아래와 같은 결과를 얻었다. 1. 어떠한 품종이던지 중앙부보다 주변 부위가 수량이 많았다. 2. 상전의 방향에 따라서 볼 때는 항상 남쪽 외측이 가장 수량이 많았고 다음이 서쪽과 동쪽 외측이며 북쪽 외측도 상당히 많았다. 각 방향의 $\frac{1}{4}$내측부분도 중앙부보다 약간 많은 경향은 있었으나 큰 차는 없었다. 품종별로 볼 때에는 개량서반과 같은 발조수가 많고 절간이 짧은 소엽형의 품종은 이와 반대의 형질을 가진 노상보다 주변효과가 크다. 5. 시기적으로 볼 때에는 개량서반은 추잠기보다 춘잠기에 있어서 주변효과가 크지만 노상은 도리어 추기가 춘기보다 약간 크다. 6. 상엽중의 건물에 있어서도 거의 같은 경향을 나타냈다. 7. 이것을 종합해 보건데 상전을 조성할 때에 있어서는 주변 효과가 적은 동서로 긴 형태로 하는 것이 증수를 하는데 유리하다.

• International Journal of Industrial Entomology and Biomaterials
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• 제47권2호
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• pp.126-133
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• 2023

Genetic resources of mulberry trees are commonly preserved as trophosomes, which are vulnerable to environmental factors, such as natural disasters, diseases, and pests. This study establishes a basic protocol for ultra-low temperature cryopreservation of mulberry trees using a two-step freezing process. The procedure was established using the "Daeshim" variety and then tested on genetic resources from 24 other mulberry varieties. Samples were first dried to a moisture content of 33-43% in a low-temperature forced-air chamber at -5 ℃, then slowly frozen from -5 ℃ to -20 ℃, and preserved in liquid nitrogen (-196 ℃). To determine the regeneration rate, isolated dormant buds were inoculated into MS basal medium, and grown shoots were grafted onto 1-year-old rootstock via chip budding and then cultured. After freezing in liquid nitrogen, the "Daeshim" variety exhibited a survival and regeneration rate of more than 70% and 50%, respectively. Applying the two-step freezing process to genetic resources from 24 mulberry species yielded average survival and regeneration rates of 85.3% and 75.5%, respectively. Morus alba showed survival and regeneration rates of 100%, confirming the efficacy of the two-step freezing method. These results indicate the high feasibility of ultra-low-temperature cryopreservation through two-step freezing of dormant buds from mulberry genetic resources. Additional research is required into the variations in regeneration rates with freezing period in liquid nitrogen.

• 한국잠사곤충학회지
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• 제42권2호
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• pp.67-72
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• 2000

Autotetraploid 'Sawonppong 26'was induced by dropping clochicine solution in growing point of Keomseolppong which is of high rootability and some agronomic charactelistics are as follows. Sawonppong 26 is a tetraploid of Keomseolppong with 56 of the chromosome number. Its fertlization percent was 59.4% and the germination percent of sank seed was normal. Sugar degree of mulberry fruit was 13.3% and total acidity was 0.69%. Single fruit weight of Sawonppong 26 was increased by 79%, as compared to that of Keomseolppong. Even though percent of survival of hard wood cutting was 94%, number of rootings was less and diameter of root was slender, it is expectedly a promising variety to be used for a breeding parent from the aspect of rootability.

• International Journal of Industrial Entomology and Biomaterials
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• 제35권1호
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• pp.45-50
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• 2017

Using the relatively low-cost, far-infrared dryer inhibiting the destruction of a variety of physiologically active components of the mulberry fruit, we have studied to make semidry mulberry fruit that can be kept at room temperature for a long time. By adjusting the temperature of the far-infrared dryer step-by-step, we developed a semi-dry method of maintaining the shape of the mulberry fruit. In addition, by drying the coating of honey after removing the juice generated by the mulberry fruit thawing process improved the acceptability of the taste of fruit. We conducted heat treatment mulberry fruit into a $95^{\circ}C$ infrared dryer 5 hours to thaw the frozen mulberry fruit. After 10 to 20% of honey coating, the primary drying ($95^{\circ}C$, 5 hours) was implemented. then, the secondary drying was conducted after controlling the temperature of the far infrared dryer $60^{\circ}C$, for 10 hours. These manufacture process was able to obtain semi-dried mulberry fruit. Dry weight ratio and moisture content were around 25%, and around 16% level respectively. It was to enable long-term storage at room temperature. Therefore, it is suggested that the method of using the far-infrared drying machine to manufacture semi-dried mulberry fruit can be a way to improve the farm income if applied to the farm.

• International Journal of Industrial Entomology and Biomaterials
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• 제16권2호
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• pp.49-56
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• 2008

A preliminary survey on the incidence and intensity of root disease complex (association of Meloidogyne incognita and root rot pathogens) was carried out in the sericultural areas of Karnataka. A total of 280 mulberry gardens were surveyed in 14 districts of Karnataka belonging to different types of soil (red sandy, red loamy and black cotton), farming systems (irrigated and rainfed), varieties (V-1, K-2, Local and S-13) and age of the plants (0-5, 5-10 and 10-15 years). It was observed that the association of M. incognita with Botryodiplodia theobromae and Fusarium solani causes the root disease complex in mulberry. Of the 280 gardens visited, 94 were infested with the disease complex and incidence was recorded as 33.6%. The higher intensity of root disease complex was observed when the root system had more than 100 galls/plant with infection of mixed population of B. theobromae and F. solani in sandy soil under irrigated farming. The 5-10 years old mulberry plantation with V-I variety was found to be most susceptible to root disease complex. Districts like Mysore, Kolar, Mandya, Tumkur, Chitradurga and Bangalore were observed as sensitive areas. Further, the wounds caused by M. incognita in mulberry roots favour the easy entry of root rot pathogens, which increased the severity of the disease very fast.