• Journal of Nutrition and Health
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• 제54권5호
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• pp.448-458
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• 2021

본 연구는 뽕나무 열매 착즙 분말 (MJ)이 지방세포 분화과정에서 염증에 관여하는 유전자 발현 및 miR-132/143 조절에 미치는 효과에 중점을 두고 조사하였다. MJ는 3T3-L1 지방세포 분화 동안 지질축적이 억제되었고 PPAR-γ, CEBP-α 및 aP2와 같은 지방형성 유전자 발현을 억제하였다. 또한 염증 조절 매개체인 TNF-α, IL-6, MCP-1 및 iNOS 유전자 발현이 MJ 처리에 의해 감소되었다. MJ의 지질 축적 억제 효과는 염증과 지방분화에 관여하는 miR-132/143의 발현 감소와 관련이 있음을 확인하였다. 따라서 MJ는 염증을 동반하는 비만 예방 및 치료에 도움이 될 수 있는 식품 소재로서 가능성이 있음을 시사하고 있다. 그러나 MJ의 주요 성분인 안토시아닌의 생체이용율은 1-2% 미만으로 추정되며 예상되는 표적 조직이나 혈류에서 미량 검출되는 것으로 알려져 있다 [33]. 이를 해결하기 위해서는 생체 내 동물실험을 통한 다각적인 분석이 향후 진행되어야 할 것으로 판단된다.

• 한국축산식품학회지
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• 제35권6호
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• pp.807-814
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• 2015

This study investigated the effect of added freeze-dried mulberry fruit juice (FDMJ) (1, 3 and 5%) on the antioxidant activity and fermented characteristic of yogurt during refrigerated storage. A decrease in pH of yogurt and increase in acidity was observed during fermentation. The yogurts with FDMJ exhibited faster rate of pH reduction than control. Initial lactic acid bacteria count of yogurt was 6.49-6.94 Log CFU/g and increased above 9 Log CFU/g in control and 1% in FDMJ yogurt for 24 h. The total polyphenol and anthocyanin content of FDMJ yogurt was higher than that of control due to the presence of phytochemical contents in mulberry. Moreover, antioxidant activity such as DPPH and reducing power was highest 5% FDMJ yogurt. During cold storage, pH decreased or remained constant in all yogurts with values ranging from 4.08 to 4.78 units. In sensory evaluation, the score of 1% FDMJ yogurt was ranked higher when compared with other yogurts. It is proposed that mulberry fruit juice powder can be used to improve sensory evaluation and enhance functionality of yogurt.

• Nutrition Research and Practice
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• 제3권4호
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• pp.272-278
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• 2009

Postprandial hypoglycemic effect of mulberry leaf (Morus alba L.) was compared in two animal models: Goto-Kakizaki (GK) rats, a spontaneous non-obese animal model for type II diabetes, and their counterpart control Wistar rats. First, the effect of a single oral administration of mulberry leaf aqueous extract (MLE) on postprandial glucose responses was determined using maltose or glucose as substrate. With maltose-loading, MLE reduced peak responses of blood glucose significantly in both GK and Wistar rats (P < 0.05), supporting the inhibition of $\alpha$-glucosidase by MLE in the small intestine. With glucose-loading, MLE also significantly reduced blood glucose concentrations, measured at 30 min, in both animal models (P < 0.01), proposing the inhibition of glucose transport by MLE. Next, dried mulberry leaf powder (MLP) was administered for 8 weeks by inclusion in the diet. By MLP administration, fasting blood glucose was significantly reduced at weeks 4 and 5 (P < 0.05), but then returned to values that were similar to those of the control at the end of experimental period in GK rats. Insulin, HOMA-IR, C-reactive protein, and triglycerides tended to be decreased by MLP treatment in GK rats. All other biochemical parameters were not changed by MLP administration in GK rats. Collectively, these findings support that MLE has significant postprandial hypoglycemic effect in both non-obese diabetic and healthy animals, which may be beneficial as food supplement to manage postprandial blood glucose. Inhibitions of glucose transport as well as $\alpha$-glucosidase in the small intestine were suggested as possible mechanisms related with the postprandial hypoglycemic effect of MLE.

• International Journal of Industrial Entomology and Biomaterials
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• 제35권2호
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• pp.89-96
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• 2017

Various health promoting effects of steamed and freeze-dried mature silkworm powder (SMSP) have been reported. However, it is not still clear which substances in SMSP are responsible for those health promoting effects yet. In this study, we examined and compared the quantities of phytochemicals in SMSP and freeze-dried mulberry leave powder (FMLP). To investigate the optimal solvent for extracting phytochemicals from SMSP and FMLP, we used four different solvents. Among them, 80% ethanol extracts from SMSP and FMLP contained the highest amount of five flavonoids examined. In addition, FMLP had high contents of flavonoids compared with those of SMSP. The amounts of rutin, isoquercetin, astragalin, quercetin, and kaempferol in FMLP were $5.078{\pm}0.187mg/g$, $5.162{\pm}0.083mg/g$, $2.989{\pm}0.061mg/g$, $3.317{\pm}0.236mg/g$, and $2.243{\pm}0.237mg/g$, respectively, while the amounts of rutin, isoquercetin, astragalin, quercetin, and kaempferol in SMSP were $0.171{\pm}0.024mg/g$, $0.252{\pm}0.032mg/g$, $0.374{\pm}0.031mg/g$, $0.645{\pm}0.063mg/g$, and $0.0512{\pm}0.047mg/g$, respectively. Taken together, SMSP could be a source for providing various and readily absorbable flavonoids.

• Nutrition Research and Practice
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• 제9권2호
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• pp.137-143
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• 2015

BACKGROUND/OBJECTIVES: Mulberry leaves contain quercetin derivatives, which have the effects of reducing obesity and improving lipid and glucose metabolism in mice with obesity. It is not clear whether or not mulberry leaves can directly affect metabolic disorders, in the presence of obesity, because of the interaction between obesity and metabolic disorders. The aim of the current study was to assess the direct action of quercetin derivatives on metabolic disorders in non-obese conditions in short-term high-fat diet fed mice. MATERIALS/METHODS: C57BL/6N mice were fed a high-fat diet, supplemented with either 0% (control), 1%, or 3% mulberry leaf powder (Mul) or 1% catechin powder for five days. Anthropometric parameters and blood biochemistry were determined, and hepatic gene expression associated with lipid and glucose metabolism was analyzed. RESULTS: Body and white fat weights did not differ among the four groups. Plasma triglycerides, total cholesterol, and free fatty acids in the 1%, 3% Mul and catechin groups did not differ significantly from those of the controls, however, plasma glucose and 8-isoprostane levels were significantly reduced. Liver gene expression of gp91phox, a main component of NADPH oxidase, was significantly down-regulated, and PPAR-${\alpha}$, related to ${\beta}$-oxidation, was significantly up-regulated. FAS and GPAT, involved in lipid metabolism, were significantly down-regulated, and Ehhadh was significantly up-regulated. Glucose-metabolism related genes, L-PK and G6Pase, were significantly down-regulated, while GK was significantly up-regulated in the two Mul groups compared to the control group. CONCLUSIONS: Our results suggest that the Mul quercetin derivatives can directly improve lipid and glucose metabolism by reducing oxidative stress and enhancing ${\beta}$-oxidation. The 1% Mul and 1% catechin groups had similar levels of polyphenol compound intake ($0.4{\times}10^{-5}$ vs $0.4{\times}10^{-5}$ mole/5 days) and exhibited similar effects, but neither showed dose-dependent effects on lipid and glucose metabolism or oxidative stress.

• 한국식품과학회지
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• 제38권6호
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• pp.756-761
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• 2006

뽕잎 분말을 첨가하여 제조한 펠릿을 서로 다른 수분 함량(14, 16, 18%), 팽화온도(220, 230, 240$^{\circ}C$), 팽화시간(4, 5, 6초)에서 제조된 뻥튀기에 대한 특성을 조사하였다. 비체적은 수분 함량, 팽화온도, 팽화시간의 증가에 따라 증가되었고 파괴력은 팽화온도와　팽화시간의 증가에 따라 증가되었지만 수분 함량의 증가에 따라 감소되는 것으로 나타났다. 색도 중 L값은 팽화온도와 팽화시간의 증가에 따라 감소되었고 a값과 b값은 팽화온도와 팽화시간의 증가에 따라 증가된 것으로 나타났으며 모두 수분 함량과는 서로 상관이 없는 것으로 나타났다. 관능검사에서는 수분 함량 18%, 팽화온도 240$^{\circ}C$, 팽화시간 4초에서 제조된 뽕잎 뻥튀기가 제일 높게 나타났고 높은 수분 함량, 높은 팽화온도, 짧은 팽화시간에서 제조된 뽕잎 뻥튀기가 전체적 기호도가 좋은 것으로 나타났다. 이러한 결과로 볼 때 높은 수분 함량, 높은 팽화온도, 짧은 팽화시간 조건으로 품질 특성이 우수한 뽕잎 뻥튀기를 제조 할 수 있을 것으로 생각된다.

• 한국식품영양과학회지
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• 제46권3호
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• pp.289-297
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• 2017

본 연구는 고지방식이로 유도된 제2형 당뇨 동물모델에서 잠상산물의 항당뇨 및 지질개선 효과를 규명하기 위하여 C57BL/6N 마우스에 14주간 잠상산물 분말(ML, SK, MF, CT)을 급여한 후 제2형 당뇨의 임상적 지표 및 인슐린 신호 전달체계에 미치는 영향을 분석하였다. HFD군보다 잠상산물을 급여한 군들의 체중증가량이 감소하는 경향이었으며, 혈장 포도당 및 인슐린 농도는 HFD군보다 ML분말을 급여한 군에서 특히 감소하였다. 혈장 T-C 농도는 HFD군에 비하여 ML과 SK 분말 급여 군에서 유의적으로 감소하였으며, 혈장 HDL-C 농도 및 HTR은 HFD군보다 ML군과 SK군에서 현저히 증가하였다. 간 조직 지질 농도는 HFD군보다 잠상산물을 급여한 네 군 모두에서 TG와 T-C 농도의 유의적인 감소가 나타났다. 이상의 결과 잠상산물 중 ML분말이 혈장 포도당, 인슐린, T-C 및 TG 농도를 가장 효과적으로 감소시켰으며, 혈장 HDL-C 농도 및 HTR은 증가시켰다. 또한, 내장지방 조직의 AKT, S6K, 그리고 AMPK의 활성도 증가시킨 것으로 보아 고지방식이에 의한 인슐린 저항성 및 지질 대사를 개선한 것으로 생각된다. 향후 ML의 인슐린 저항성 개선 효과에 대해 더 깊이 있는 기전연구가 수행된다면 제2형 당뇨환자를 위한 건강기능성 식품으로 개발될 수 있을 것으로 생각한다.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권2호
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• pp.207-210
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• 2004

Artificial diet (AD) has shown with different advantages over mulberry leaves (ML). Various types of such feed for silkworm have been developed and proposed. The aims of this study were possibility rearing whole instar of silkworm on AD and to find some economic formulation for rearing silkworm, compare to that ML. Eleven ADs (D$_1$ to D$_{11}$) were prepared with different percentage of mulberry leaf powder, and other ingredients such as soybean meal, cellulose, potato starch, agar, wheat flour, rice bran, etc. Five formulated diets (D$_1$ D$_2$, D$_{5}$, D$_{10}$ and D$_{11}$) appeared to have similar response to that ML (D$_{m}$). There were no significant differences for duration period between ADs, all of which recorded with higher duration than mulberry leaves. Compared to other died used in the present study D$_1$ and D$_{10}$ showed better growth development and survival rate. Larvae fed D$_1$, D$_2$, D$_{5}$, D$_{10}$ and D$_{11}$ grew faster and produced heavier and more useful cocoons.coons.coons.coons.

• 한국작물학회지
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• 제56권1호
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• pp.18-22
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• 2011

뽕나무 오디함유 천연색소 C3G의 식용색소화 및 안정생산을 위한 최적 전처리 조건은 0.1 % citric acid-70% EtOH로서 오디로부터 제조한 순수 오디색소 분말의 수율은 8%이었다. 실제 농가에서 오디 색소 추출시 추출 시간 및 제조 비용을 대폭 절감할 수 있는 추출 방법을 개발하였다. 오디색소 추출시 품종의 선택이 중요하므로 C3G 함량이 높은 품종을 선택하도록 해야 할 것이다. 오디 색소 분말은 알코올, 물 및 밀가루 등 식품 재료나 가공방법에 상관없이 적색소의 특성이 유지되므로 다양한 식품에 적용하여 고부가가치를 향상시킬 수 있을 것으로 판단된다.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.125-125
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• 2002

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