• Title/Summary/Keyword: mud layer

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Biocontrol Activity of Bacillus amyloliquefaciens CNU114001 against Fungal Plant Diseases

  • Ji, Seung Hyun;Paul, Narayan Chandra;Deng, Jian Xin;Kim, Young Sook;Yun, Bong-Sik;Yu, Seung Hun
    • Mycobiology
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    • v.41 no.4
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    • pp.234-242
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    • 2013
  • A total of 62 bacterial isolates were obtained from Gomsohang mud flat, Mohang mud flat, and Jeju Island, Republic of Korea. Among them, the isolate CNU114001 showed significant antagonistic activity against pathogenic fungi by dual culture method. The isolate CNU114001 was identified as Bacillus amyloliquefaciens by morphological observation and molecular data analysis, including 16SrDNA and gyraseA (gyrA) gene sequences. Antifungal substances of the isolate were extracted and purified by silica gel column chromatography, thin layer chromatography, and high performance liquid chromatography. The heat and UV ray stable compound was identified as iturin, a lipopeptide (LP). The isolate CNU114001 showed broad spectrum activity against 12 phytopathogenic fungi by dual culture method. The semi purified compound significantly inhibits the mycelial growth of pathogenic fungi (Alternaria panax, Botrytis cinera, Colletotrichum orbiculare, Penicillium digitatum, Pyricularia grisea and Sclerotinia sclerotiorum) at 200 ppm concentration. Spore germ tube elongation of Botrytis cinerea was inhibited by culture filtrate of the isolate. Crude antifungal substance showed antagonistic activity against cucumber scleotiorum rot in laboratory, and showed antagonistic activity against tomato gray mold, cucumber, and pumpkin powdery mildew in greenhouse condition.

The conservation processing of archaeological wooden artifacts excavated from The HAMAN sungsan mountain fortress wall (함안 성산산성 출토 목제유물의 보존처리)

  • Ahn, Bok-Jun;Yang, Seok-Jin
    • 보존과학연구
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    • s.25
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    • pp.155-170
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    • 2004
  • Jonam mountain is located in Gung nam Hamangun gayaeup, and it is 139.4m above sea. Most of Haman area consists of hill and mountains and Pinus densiflora Sieb. et Zucc,Quercus acutissima Carruth, Quercus variabilis Bl, Quercus mongolica Fisch and Robiniapseudoacacia L are distributed. Sungsan mountain fortress wall is designated as a national historical spot 67th and our laboratory excavated and surveyed Sungsan mountain fortress wall from 1991 to 1994.Especially, we confirmed mud layer in 1992 and excavated wooden remains such as wooden strip with inscription and so on. Also, wooden strip with inscription and the wooden remains of the type which is various such as brush, knife, various club, vessel, awl were excavated in 2002.After classifying remains on the spot, they were washed. We found three pieces of wooden strip during washing, and three pieces of wooden strip during conservation processing. Also, we confirmed others. Until now, we have processed 8000 wooden remains, and we are processing about 1500wooden remains.

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Net Radiation and Soil Heat Fluxes Measured on Coastal Wetland Covered with Reeds (갈대 서식 연안습지에서의 순복사와 토양열 플럭스)

  • Kim, Hee-Jong;Kim, Dong-Su;Yoon, Ill-Hee;Lee, Dong-In;Kwon, Byung-Hyuk
    • Journal of Environmental Science International
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    • v.16 no.2
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    • pp.233-239
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    • 2007
  • In the coastal wetland the mud is consist of fine particles, which means that it is characterized by small gap, and heat transfer is obstructed since moisture is found between the gaps. The relationship between net radiation ($R_N$) and soil heat flux($H_G$) shows a counterclockwise hysteresis cycle, which refer to a time lag behind in the maximal soil heat fluxes. The albedo is independent of seasonal variation of the vegetation canopy which plays very important roles to store and control the heat in the atmospheric surface layer.

Study on the Long-term Change of nitrogen in the Tidal Area of River (하천 감조부에 있어서 질소의 장기변동에 관한 연구)

  • 김원규;강주복
    • Water for future
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    • v.25 no.2
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    • pp.67-78
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    • 1992
  • Several field surveys were conducted to investigate changes of water quality with time in a tidal river. Results indicated that nitrification process were dependent on the change of salinity and suspended solids concenttration. Therefore laboratory batch experiments were conducted, using suspended solids and sediment taken from a tidal river, to study the effect of salinity on nitrification and to estimate kinetic parameters of it in the tidal river. suspended solids and sediment were sampled at a point in the middle stream. Sediments were collect from the aerobic layer of mud. The change of nitrogen concentration with time was clearly explained with Monod groth model and kinetic parameters were obtained by curve fitting method. Changes in NH4-N, NO2-N, and NO3-N concentrations in the river ROKKAU with time were simulated well using Lagrangian reference frame and parameter values obtained in the laboratory tests. T도 mechanism of nitrification by suspended solids and sediment in a tidal river is shown to depend on tidal effects.

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Preliminary Comparison of Deep-sea Sedimentation in the Ulleung and Shikoku Basins: Deep-sea Circulations and Bottom Current (울릉분지와 시코쿠분지 심해퇴적작용의 비교에 관한 기초연구: 심층수순환과 저층류)

  • Chun, Seung-Soo;Lee, In-Tae
    • Journal of the Korean earth science society
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    • v.23 no.3
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    • pp.259-269
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    • 2002
  • Based on sedimentary structures, degree of bioturbation, and internal erosional layers, the deep-sea core sediments in the East Sea (Ulleung and Yamato basins) and the Northwestern Pacific Ocean (Shikoku Basin) can be divided into two parts (upper and lower) with the boundary of around 10,000 years B.P. in age. The upper part of core KT94-10 from Shikoku Basin is characterized by low sedimentation rate, internal erosion layer, high degree of bioturbation and cross-lamination structures. It can be interpreted as the bottom-current deposits which show some different characteristics from turbidite or hemipelagic sediment. However, its lower part consists of highly bioturbated, massive mud, suggesting that it be not related to the influence of bottom current. On the other hand, the cores in Ulleung and Yamato basins do not show any evidence of bottom-current deposits: their upper parts consist of bioturbated mud, and lower parts are characterized by laminated mud with pyrite filaments, indicating anaerobic condition. Consequently, these sedimentological characteristics suggest that deep-sea circulation would be changed from slow-moving to fast-moving one at this bounding time commonly in the Northwestern Pacific Ocean and the East Sea. Also, even in the same time, the deep-sea circulation in the Northwestern Pacific area would be relatively faster than that in the East Sea.

The Study on Removing Paraloid B-72 from Painting Layer on Mural of Mireukjeon Hall at Geunsansa Temple (금산사 미륵전 벽화 채색층의 Paraloid b-72 제거방법과 안정성에 관한 연구)

  • Jin, Byung-Hyuk;Cho, Jae-Yeon;Park, Jin-Yeon;Han, Sung-Hee;Kim, Yong-Sun
    • Korean Journal of Heritage: History & Science
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    • v.50 no.3
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    • pp.88-109
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    • 2017
  • As the technique to remove Paraloid B-72, which is known as an irreversible material, the method using organic solvent and heating, though the ways vary depending on the kind of material to be removed, has been usually used, but it has yet to apply to mud mural because of the technical limit in processing and the potential risk of damage and, moreover, the removal efficiency which also remains unproven. Thus, in a bid to seek the way to safely remove Paraloid B-72 contained in mural, the test was conducted in a way of applying a compress method, which is deemed most efficient. The solvents which are proven to be Paraloid B-72 were applied to the absorbents such as active carbon fiber and methyl cellulose and then were eluted to the surface of mud mural sample which was prepared in the same size and condition for a certain time before evaluating the stability and removal efficiency. Such test was intended to identify the applicability to the mural of Mireukjeon Hall at Geunsansa Temple, which had been treated with Paraloid B-72 for preservation in the past. As a result, the way of mixing the absorbent such as active carbon fiber and Xylene alone or with other quick vaporable solvents proved to be most efficient in removing Paraloid B-72 from mud mural and particularly Acetone:Xylene(1:1wt%) was found to be the most stable among others. Such a test outcome is expected to be a useful data for removing Paraloid B-72 from the mural of Mireukjeon Hall at Geunsansa Temple as well as for restoring other mural cultural assets in the coming days.

Age Dating and Paleoenvironmental Changes of the Kunang Cave Paleolithic Site

  • Yum, Jong-Kwon;Lee, Yung-Jo;Kim, Jong-Chan;Kim, In-Chul;Kim, Ju-Yong
    • The Korean Journal of Quaternary Research
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    • v.17 no.2
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    • pp.145-148
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    • 2003
  • The Kunang cave paleolithic site is located at Tanyang [$N37^{\circ}2'$, $128^{\circ}21'E$], Chungbuk Province, which is in the Central part of the Korean peninsula. The cave is developed at 312 amsl in a karstic mountainous area. The South Han River flows across this region and other caves can also be found near the river. The site was discovered in 1986 and excavated 3 times by the Chungbuk National University Museum until now. The cave was wellpreserved from modem human activities until the first discovery. The full length of the cave is estimated to be ca. 140 m. However, a spacious part up to 11 m from the entrance has been excavated. Eight lithological units are divided over the vertical profile at a depth of 5 m. Each unit is deposited in ascending order as follow: mud layer (Unit 9), lower complex (Unit 8) which is composed of angular blocks and fragments with a muddy matrix, lower travertine layer (Unit 7; flowstone), middle complex (Unit 6; cultural layer) which is composed of fragments with a muddy matrix, middle travertine layer (Unit 5; flowstone), yellowish muddy layer (Unit 4), upper complex (Unit 3; cultural layer) which has a similar composition to Unit 8. the upper travertine layer (Unit 2; flowstone), and finally surface soil layer (Unit 1). The most abundant vestiges in the cultural layers are the animal bones. They are small fractured pieces and mostly less than 3 cm in length. About 3,800 bone pieces from 25 animal species have been collected so far, 90 percent of them belonging to young deers. Previous archaeological study of these bone pieces shows thatprehistoric people occupied the cavenot for permanent dwelling but for temporary shelter during their seasonal hunting activity. More extensive studies of these bones together with pollen analysis are in progress to reconstruct the paleoenvironment of this cave. Only a single date (12,500 BP) obtained from a U-Th measurement of the upper travertine layer was previously available. In spite of the importance of the cave stratigraphy, there was no detail chronological investigation to establish the depositional process of the cultural layers and to understand the periodic structure of the cave strata, alternating travertine floor and complex layers. We have measured five 14C age dating (38900+/-1000, 36400+/-900, 40600+/-1600, more than 51000 and 52000 14C BP) using Seoul National University 14C AMS facility, conducted systematic process of the collagen extraction from bone fragments samples. From the result, we estimate that sedimentation rate of the cave earth is constant, and that the travertine layers, Unit 2 and Unit 3, was formed during MIS 5a(ca. 80 kBP) and MIS 5c (ca. 100 kBP) respectively. The Kunang Cave site is located at Yochonli of the region of Danyang in the mid-eastern part of Korea. This region is compased of limestones in which many caves were found and the Nam-han river flows meanderingly. The excavations were carried out three times in 1986, 1988, and 1998.

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Seasonal Change of Skin Mucus Cells of Misgurnus mizolepis (Cobitidae) Dwelling in a Natural Stream in Korea (자연하천에 서식하는 미꾸라지 Misgurnus mizolepis 피부 점액세포의 계절변화)

  • Oh, Min-Ki;Park, Jong-Young
    • Korean Journal of Ichthyology
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    • v.22 no.4
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    • pp.230-237
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    • 2010
  • Histological observation on the seasonal variation of mucus cells of the mud loach Misgurnus mizolepis inhabiting a natural stream was carried out on three skin regions (dorsal, lateral and occiput) from March 2008 to February 2009. Our results showed no differences in general morphology by season, but the mucus cells of the epidermis showed significant seasonal change in their size and number as the water temperature changed. The ratio of surface area of the mucus cell layer and mucus cells, and the number of mucus cells in surface area of the epidermis were the greatest in the cold winter and the least in the hot summer in all regions of the epidermis. In particular, the occiput seemed to be a very sensitive region in response to environmental change, showing wide fluctuations in the size of mucus cells throughout the year and a great change in between seasons, especially from late autumn to early winter when the temperature decreased. As the temperature became colder, a small and spherical-shaped mucus cell was transformed into a large and elongated columnar form with a lot of secreted mucus material in a superficial layer of the epidermis. From our results, we can safely surmise that cold temperature is an important environmental factor having a close relationship with the modification of mucus cells of M. mizolepis in winter.

A Study on the Particle Size of Sand to Prevent Penetration of Subterranean Termite (Reticulitermes speratus kyushuensis) in Wooden Buildings (국내 지중흰개미의 목조건축물 유입 차단을 위한 모래의 적정 입도 연구)

  • Kim, Si Hyun;Kim, Tae Heon;Chung, Yong Jae
    • Journal of Conservation Science
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    • v.38 no.2
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    • pp.80-86
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    • 2022
  • Termites cause massive damage to wooden architectural heritage structures. Chemical treatments have been commonly used to control them. In foreign countries, physical barriers made of sheet and particles impenetrable to termite are being used as an alternative to the chemical method. To study the efficacy of physical barriers, we investigated the appropriate sand particle size that can prevent the penetration of R. speratus kyushuensis. Upon evaluating the barrier properties of sand with particle sizes ranging from 0.85 to 4.00 mm, the penetration of termites was found to be effectively blocked at a particle size range of 1.00 to 2.80 mm. At smaller particle sizes, termites managed to move the sand particles and build an almost linear mud tube to penetrate the sand layer. At larger particle sizes, the termites could penetrate the sand layer by passing through the sand gaps.

Seasonal Variations of the Heat Flux in Muddy Intertidal Sediments near the Jebu Island during the Ebb Tides in the West Coast of Korea (서해 제부도 해역의 간조시 갯벌 퇴적층내 지온 및 열수지의 계절변화)

  • Na, Jung-Yul;Yu, Sung-Hyup;Seo, Jang-Won
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.5 no.1
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    • pp.1-9
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    • 2000
  • Vertical temperature distributions in muddy intertidal sediments near the Jebu Island on the west coast of Korea were obtained during the period of ebb tide which occurred in day time. The observations of mud temperature were made with thermistor embedded probe at 2cm interval for 18cm-layer of sediment for five different months of the year. Temporal changes in the vertical profile of the sediment temperature are strongly depend on the air temperature, the previous time of flood tide and the time of ebb tide. Heat exchanges in the surface layer (0-2 cm) in terms of magnitude and direction are greater than and opposite to those in the deeper sediment layer (8-12 cm), respectively and do not show any significant seasonal variations. In general, the surface layer gains heat while the deeper layer loses the heat. By using the 1-D diffusion equation temporal vertical profiles of the sediment temperature were obtained and were compared with the observed ones. The results show that in the sediment layer below 4 cm-depth the heat transport is predominantly by molecular diffusion. The average magnitude of heat flux into the sediment layer (0-18 cm) during the ebb tide when the mudflats were exposed in the middle of the day were between 4.1 and $28.9\;W/m^2$.

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