• The Journal of Dong Guk Oriental Medicine
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• v.2 no.2
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• pp.69-84
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• 1993

This study was performed to identify the effect of Backchulsan and Backpeundu, on the muco-substances of the goblet cells in the small and large intestines of the mouse. After injected castor oil into oral cavity, the experimental animals were administrated with 5.952mg per 30g body weight of Backchulsan, with 0.519mg per 30g body weight of Backpeundu. The specimens used in this study were obtained from the small and large intestines of the mouse. All specimens were stained with periodic acid-Schiff reaction, alcian blue pH 2.5 and alcian blue pH 1.0 procedures. The results were as follows : 1. The most remarkable decrease of neutral and acid mucosubstance was observed in the control group 1 and 6 hours after castor oil administration. 2. Increase of neutral and mucosubstance was observed in Backchulsan administration group 3 hours in comparision with that of control group administrated of castor oil, especially of the upper and lower villi regions. 3. Increase of neutral and acid mucosubstance was observed in Backpeundu administration group 6 hours in comparision with that of control group administration of castor oil. 4. The most remarkable increase of neutral and acid mucosubstance was observed in Backchulsan administration group after 3 hours in comparision with that of Backpeundu administration group.

• Animal cells and systems
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• v.12 no.2
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• pp.85-91
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• 2008

Structure and mucosubstance histochemistry in the epidermis of five teleostean species, i. e., spotty belly, Agrammus agrammus, devil stinger, Inimicus japonicus, stone fish, Erosa erosa, cubed snailfish, Liparis tessellatus, and Japanese bluefish, Scombrops boops were investigated. The epidermis of five species studied is composed of three layers: superficial, middle, and basal layer. The superficial layer is comprised of rather flattened cells. Mucous cells, the type commonly found in fishes are completely lacking in the epidermis of devil stinger, stone fish, and cubed snailfish. The epidermis of devil stinger and stone fish have multicellular glands which do not have mucosubstances. The skin surface of them is covered with mucous layer. The superficial cells in the epidermis of devil stinger, stone fish, and cubed snailfish are mucus-secreting cells. The composition and the amount of the mucosubstances vary in species and body regions. The mucous layer on the skin surface and superficial epidermal cells of devil stinger contain a mixture of neutral and acidic(sulfated and nonsulfated) mucosubstances. In stone fish, the mucous layer has acidic(sulfated and non-sulfated) mucin and the superficial epidermal cells contain neutral mucin. In cubed snailfish, the type of epidermal mucosubstances is identified as a mixture of neutral and acidic(non-sulfated) mucin. The mucous cells of the epidermis in spotty belly and Japanese bluefish contain neutral mucin.

• The Journal of Dong Guk Oriental Medicine
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• v.7 no.1
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• pp.119-133
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• 1998

This study was performed to investigated the effects of an immunosuppression and a mitigation of inflammation of Scutellaria baicalensis GEORGI on Allergic contact dermatitis. In order to study, after oral administration of Scutellaria baicalensis GEORGI extract, contact hypersensitivity assay, general morphologic change of skin, sulfated acid mucosubstance, mast cell, IL-2R, ICAM-1 and CD11b were observed in BALB/C mouse induced allergic contact dermatitis by the contact sensitizing DNCB. Ear swelling in contact hypersensitivity assay was decreased in the Scutellaria baicalensis GEORGI extracts administered(HEGT) group, compared with DNCB painted group. In the general morphologic change of skin, hyperplasia of keratinocytes, increase of lymphocytes and inflammatory cell, increase of vasculogenesis and epidermal lymphocytes infiltration, increase of cell damage in epidermal basal layer and prickle layer were decreased in the HEGT group, compared with DNCB painted group. Increase of sulfated acid mucosubstance, mast cell, IL-2R positive cell, ICAM-1 positive cell, CD11b positive cell were decreased in the HEGT group, compared with DNCB painted group.

• Animal cells and systems
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• v.11 no.2
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• pp.183-189
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• 2007

To investigate the properties of mucosubstances of the epidermis in various teleostean species, conventional histochemical stainings were used on the skin in five species of order Perciformes, i. e., yellowtail, Seriota quinqueradiat, striped beakperch, Oplegnathus fasciatus, brown spotted grouper, Epinephelus chlorostigma, sea chub, Ditrema temmincki and multicolorfin rainbowfish, Halichoeres poecilopterus. The following methods were used: periodic acid Schiff (PAS), alcian blue (AB) pH at 2.5, AB pH at 1.0, AB pH at 2.5-PAS, AB pH at 1.0-PAS, aldehyde fuchsin (AF) pH at 1.7-AB pH at 2.5 and high iron diamine (HID)-AB pH at 2.5. The epidermis of all five species consisted of three layers: superficial, middle, and basal layer. The superficial layer was comprised of rather flattened cells. In particular, the outermost layer of striped beakperch and middle layer of sea chub consisted of mucus-secreting cells. Mucous cells, the unicellular glands, were found in epidermis but varied in number in different body regions and species. Although there was a slight difference in the amount in various species and body regions, the secretory contents of the mucous cells in the five teleostean species contained acidic mucopolysaccharides. In yellowtail, striped beakperch, and multicolorfin rainbowfish, the property of mucosubstances was identified as sialomucin, while it was sulphomucin in brown spotted grouper and sea chub.

• Applied Microscopy
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• v.31 no.4
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• pp.325-331
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• 2001

Integumentary structures of the stone flounder, Karefus bicoloratus were examined by means of the light and transmission electron microscopy. Stratified epidermal layer consists of supporting cells, unicellular glands and granular cells. The epidermal layer could be classified into superficial, intermediated and basal layer by morphology and structure of the supporting cells . The cytoplasm of supporting cells is divided into cortex and medullar part. In the cortex microfilaments are well developed. Mucous cells of unicellular gland were observed in the superficial and intermediated layer of the epidermis. The mucous materials were identified as glycoprotein of neutral and carboxylated mucosubstance by histochemical methods. Club cell has well developed smooth endoplasmic reticula and Golgi complex in the cytoplasm. Granular cells were observed in the intermediated and basal layer, and the cytoplasm is occupied with membrane-bounded granules of electron dense. Three types of pigment cells could be distinguished with electron density of cytoplasmic inclusions. Nerve myelins were observed near the pigment cells.

• Journal of Veterinary Clinics
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• v.16 no.2
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• pp.339-351
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• 1999

Excess secretion of goblet cell stimulated by inhalation anesthetics have side effects during operation or postoperative care. Mucosubstances, which are almost secreted by goblet cells in the epithelium of the respiratory tract, are secreted by a direct irritation of inhalation anesthetics. This study was carried out to compare the differences of mucus secretion on lower respiratory tract stimulated by ethyl ether, halothane and isoflurane. Total of 24 rabbits were used as experimental animals. The trachea and the 1st bronchi were fixed in 10% neutral buffered formalin. After embedding in paraffin, the specimens were sectioned to a thickness of 6 ${\mu}{\textrm}{m}$, and PAS-H, Alcian blue pH 2.5 and Alcian blue pH 1.0 stains were performed for the observation of the composition and the quantity of the mucus. The results were as follows; Ethyl ether and isoflurane irritated the mucous membrane of the respiratory tract. Ethyl ether irritated more than isoflurane. Halothane irritated the mucous membrane, but its effect was minimal and had little influences during operation. In the specimens stained with PAS-H, Alcian blue pH 2.5 and Alcian blue pH 1.0, the mucosubstance lining the cilia and in the goblet cells of the trachea and 1st bronchi were the strongly PAS-H reactive mucosubstances, moderately Alcian blue pH 2.5 and Alcian blue pH 1.0. The PAS-H reactive mucosubstance were polysaccharides, neutral mucopolysaccharides, mucoproteins, glycoproteins and glycolipids. Trachea was easily affected than bronchi by inhalation anesthetics. Consequently, it is suggested that because halothane does not irritates respiratory mucosal secretion, its application may be efficient to the depressed respiratory system.

• Animal cells and systems
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• v.3 no.3
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• pp.253-258
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• 1999

The present study describes histochemical properties of the mucosubstances in the pedal sole of abalone, in relation to two main additional functions known to date. Only neutral mucosubstances were demonstrated in the lateral epithelium of the pedal sole of the abalone, however, two distinct types of cells, one containing neutral and the other containing acidic mucosubstances, were identified in the ventral epithelium. The epithelial mucocytes distributed throughout both epithelial and subepithelial glands located in the ventral subepithelial region showed a mixture of neutral and acidic mucosubstances. However, acidic mucosubstances were found in larger amounts than neutral ones in epithelial mucocytes, and vice versa in subepithelial glands. Among these acidic mucosubstances, sulphated substances were dominant. Lesser amounts of carboxylated substances also occurred together with low levels of neuramic and sialic acid. Collectively, the notable morphological differences between abalone and other gastropoda were observed in the ventral surface of the foot, which showed a tall columnar epithelium, peripherally concentrated mucocytes, and subepithelial glands. Mucosubstances are presumably produced by these cells and may also play a role in clinging to some substances to support abalones in addition to the main functions of lubrication and protection.

• Korean Journal of Ichthyology
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• v.12 no.1
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• pp.25-32
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• 2000

The structure of skin was studied in Iksookimia longicorpus based on the micro-anatomical investigation of skin fragments taken from four regions. The epidermis was distinguished by two types of skin glands, a small mucous cell and a large club cell. The mucous cell was acid sulfomucins (some sialomueins) but the club cell did not give any histochemical tests for mucosubstances. The presence of a well defined lymphatic system with small lymphocytes was established in the stratum germinativum layer of the epidermis. A large number of blood capillaries run very close to each other just below the basement membrane, and a definite area giving AB and PAS positive was present between the basement membrane and scale.

• The Korean Journal of Malacology
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• v.19 no.2
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• pp.81-87
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• 2003

The digestive system of the abalone, Haliotis discus hannai consists of radula sac, esophagus, crop, stomach, intestine (anterior, mid and posterior intestine) and hepatopancreas. The epithelial layer was composed of ciliated columnar cells, mucous cells and granular cells. And epithelium thickness of the crop was thicker (90.80 ${\mu}$m) than those of other regions. Mucous cells of PAS positive in the esophagus were more advanced than those of other regions. The contents of mucous cell were neutral and acid mucosubstance in the esophagus and the anterior, mid and posterior intestine. And it seem to be lipid in the crop and stomach.

• Biomolecules & Therapeutics
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• v.9 no.4
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• pp.263-269
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• 2001

In this study, we tried to investigate whether poly-L-arginine (PLA) (MW 10,800) significantly affect mucin release from cultured hamster airway goblet cells and the mucosubstances of hypersecretory air-way goblet cells of rats. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with $^3$H-glucosamine for 24 hr and chased for 30 min in the presence of varying concentrations of PLA to assess the effects on $^3$H-mucin release. Possible cytotoxicities of PLA were assessed by measuring both Lactate Dehydrogenate (LDH) release and by checking the possible changes on the morphology of HTSE cells during treatment. For in vivo experiment, hyperplasia of rat airway goblet cells and increase in intraepithelial mucosubstances were induced by exposing rats to SO$_2$ for 3 weeks and varying concentrations of PLA were administered inhalationally to assess the effects on the mucosubstances of airway goblet cells of rats. The results were as follows : (1) PLA significantly inhibited mucin release from cultured HTSE cells in a dose-dependent manner; (2) there was no significant release of LDH and no significant change on the morphology of cultured HTSE cells during treatment; (3) PLA also affected the intraepithelial mucosubstances of hypersecretory rats and restored them to the levels of control animals. We conclude that PLA inhibit mucin release from airway goblet cells without significant cytotoxicity and possibly normalize the hypersecretion of airway mucosubstances in vivo. This finding suggests that PLA might function as an airway mucoregulative agent.