• BMB Reports
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• v.52 no.10
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• pp.613-618
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• 2019

Microglial cells are known as the main immune cells in the central nervous system, both regulating its immune response and maintaining its homeostasis. Furthermore, the antioxidant ${\alpha}-lipoic$ acid (LA) is a recognized therapeutic drug for diabetes because it can easily invade the blood-brain barrier. This study investigated the effect of ${\alpha}-LA$ on the inflammatory response in lipopolysaccharide (LPS)-treated BV-2 microglial cells. Our results revealed that ${\alpha}-LA$ significantly attenuated several inflammatory responses in BV-2 microglial cells, including pro-inflammatory cytokines, such as tumor necrosis $factor-{\alpha}$ and interleukin (IL)-6, and other cytotoxic molecules, such as nitric oxide and reactive oxygen species. In addition, ${\alpha}-LA$ inhibited the LPS-induced phosphorylation of ERK and p38 and its pharmacological properties were facilitated via the inhibition of the nuclear factor kappa B signaling pathway. Moreover, ${\alpha}-LA$ suppressed the activation of NOD-like receptor pyrin domain containing 3 (NLRP3) inflammasomes, multiprotein complexes consisting of NLRP3 and caspase-1, which are involved in the innate immune response. Finally, ${\alpha}-LA$ decreased the genes accountable for the M1 phenotype, $IL-1{\beta}$ and ICAM1, whereas it increased the genes responsible for the M2 phenotype, MRC1 and ARG1. These findings suggest that ${\alpha}-LA$ alleviates the neuroinflammatory response by regulating microglial polarization.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.21 no.1
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• pp.43-48
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• 2021

One of the effective ways to increase data transmission rate is to use massive antenna technique where tens or hundreds of antennas are deployed in base station and spatial diversity gain is improved by multiuser method. If multiuser method is applied, there will be inter-user interference and maximal ratio combiner (MRC) is conventionally used to reduce the complexity of the receiver and to eliminate interference. However, as the number of mobile devices increases, the performance of the conventional receiver becomes deteriorated. To solve this problem, we propose a new detector that completely eliminates the interference from the registered devices and reduces that from the unregistered devices. Then, to reduce the complexity of the proposed scheme, we propose adaptive algorithm of the proposed scheme. Through simulation, we show that the proposed scheme has better bit error rate performance than the conventional scheme.

• Journal of Information Technology Services
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• v.20 no.1
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• pp.131-143
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• 2021

Commercial transactions, one of the pillars of the capitalist economy, are occurring countless times every day, especially small and medium-sized businesses. However, small and medium-sized enterprises are bound to be the legal underdogs in contracts for commercial transactions and do not receive legal support for contracts for fair and legitimate commercial transactions. When subcontracting contracts are concluded among small and medium-sized enterprises, 58.2% of them do not apply standard contracts and sign contracts that have not undergone legal review. In order to support small and medium-sized enterprises' fair and legitimate contracts, small and medium-sized enterprises can be protected from legal threats if they can reduce the risk of signing contracts by analyzing various risks in the contract and analyzing and informing them of toxic clauses and omitted contracts in advance. We propose a risk prediction model for the machine reading-based legal contract to minimize legal damage to small and medium-sized business owners in the legal blind spots. We have established our own set of legal questions and answers based on the legal data disclosed for the purpose of building a model specialized in legal contracts. Quantitative verification was carried out through indicators such as EM and F1 Score by applying pine tuning and hostile learning to pre-learned machine reading models. The highest F1 score was 87.93, with an EM value of 72.41.

• Journal of Physiology & Pathology in Korean Medicine
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• v.36 no.1
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• pp.1-6
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• 2022

The tuber of Pinellia ternata (Thunb.) Brei (TPT) used in traditional Oriental medicine for the treatment of cough, sputum, vomiting, and insomnia, possesses antioxidant, antibacterial, and anti-inflammatory effects. Although recent studies have reported the anticancer effects of TPT in several cancer cells, it is still unclear whether TPT regulates tumor-associated macrophage (TAM) characterized by the immunosuppressive M2 macrophage phenotype. Our results showed that the ethanol extract of TPT (ETPT) suppressed the migration of RAW264.7 mouse macrophage cells and THP-1 human monocytes differentiated into macrophages towards the conditioned media (CM) collected from lung cancer cells, suggesting that ETPT would attenuate the recruitment of macrophages into tumors. In addition, ETPT suppressed the interleukin (IL)-4 or IL-6-induced M2 macrophage polarization in RAW264.7 cells. ETPT treatment not only downregulated the mRNA expression of M2 macrophage markers including arginase-1, mannose receptor C type 1 (MRC-1), and IL-10, but also inhibited the phosphorylation of signal transducer and activator of transcription 3 (STAT3) and STAT6, general regulators of M2 macrophage polarization. Finally, the transwell assay results showed that the CM from M2-polarized RAW264.7 cells increased the migration of mouse lewis lung carcinoma (LLC) cells, while those from RAW264.7 cells co-treated with ETPT and IL-6 significantly reduced the migration of LLC cells. Taken together, our observations clearly demonstrate that ETPT suppressed the cancer cell migration by regulating macrophage recruitment and M2 macrophage polarization.

• Journal of Internet of Things and Convergence
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• v.8 no.5
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• pp.17-25
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• 2022

Recently the massive amount of data has been generated because of the number of edge devices increases. And especially, the number of raw unstructured HTML documents has been increased. Therefore, MRC(Machine Reading Comprehension) in which a natural language processing model finds the important information within an HTML document is becoming more important. In this paper, we propose HTDE(HTML Tag Depth Embedding Method), which allows the BERT to train the depth of the HTML document structure. HTDE makes a tag stack from the HTML document for each input token in the BERT and then extracts the depth information. After that, we add a HTML embedding layer that takes the depth of the token as input to the step of input embedding of BERT. Since tokenization using HTDE identifies the HTML document structures through the relationship of surrounding tokens, HTDE improves the accuracy of BERT for HTML documents. Finally, we demonstrated that the proposed idea showing the higher accuracy compared than the accuracy using the conventional embedding of BERT.

• Journal of Microbiology and Biotechnology
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• v.33 no.7
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• pp.864-874
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• 2023

Natural killer (NK) cell dysfunctions against hepatocellular carcinoma (HCC) in a hypoxic environment. Many solid tumors are present in a hypoxic condition, which changes the effector function of various immune cells. The transcription of hypoxic-inducible factors (HIFs) in cancer cells make it possible to adapt to their hypoxic environment and to escape the immune surveillance of NK cells. Recently, the correlation between the transcription of HIF-1α and pro-inflammatory cytokines has been reported. Interleukin (IL)-6 is higher in cancers with a highly invasive ability, and is closely related to the metastasis of cancers. This study showed that the expression of HIF-1α in HCC cells was associated with the presence of IL-6 in the environment of HCC-NK cells. Blocking of IL-6 by antibody in the HCC-NK interaction changed the production of several cytokines including TGF-β, IL-1, IL-18 and IL-21. Interestingly, in a co-culture of HIF-1α-expressed HCC cells and NK cells, blocking of IL-6 increased the production of IL-21 in their supernatants. In addition, the absence of IL-6 significantly enhanced the cytotoxic ability and the expression of the activating receptors (NKG2D, NKp44, and NKG2C) in NK cells to HIF-1α-expressed HCC cells. These effects might be made by the decreased expression of HIF-1α in HCC cells through the inhibited phosphorylation of STAT3. In conclusion, the absence of IL-6 in the interaction of HIF-1α-expressed HCC cells and NK cells could enhance the antitumor activity of NK cells to HCC cells.

• International journal of advanced smart convergence
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• v.12 no.1
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• pp.140-148
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• 2023

With the OTT media market growing rapidly, the significance of trustworthy data verification and certification cannot be emphasized enough. This study delves into the crucial need for such measures in South Korea, exploring the steps involved, the technological and policy-related considerations, and the challenges that may arise once these measures are put into place. Drawing on in-depth interviews and the analytical hierarchy process (AHP), this study surveyed various stakeholder groups, both directly and indirectly related to OTT data authentication and certification. By assessing the severity of OTT data-related issues and identifying the requirements for reliability-improvement policies, participants shared their valuable insights and opinions on this pressing matter. The survey results clearly indicate a divided opinion among stakeholders and industry experts on the reliability of OTT data, with some expressing trust while others remain skeptical. However, there was a consensus that advertising-based AVOD is more reliable than SVOD. By analyzing the priorities of authentication and verification, this study paves the way for the establishment and operation of a Korean MRC (KMRC), centered on the OTT media industry. The KMRC will serve as a vital platform for ensuring the authenticity and accuracy of OTT data in South Korea, providing businesses and industry players with a reliable source of information for informed decision-making. This study highlights the pressing need for reliable data authentication and certification in the rapidly growing OTT media market, and provides a persuasive case for the establishment of a KMRC in South Korea to meet this critical need.

• Journal of Life Science
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• v.26 no.11
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• pp.1320-1329
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• 2016

The present study examined the cytotoxic effects of 1, 2, 3, 4, 6-penta-O-galloyl-${\beta}$-D-glucose (PGG), known as the pentahydroxy gallic acid ester of glucose, in the various human cancer cell lines (A-549, MDA-MB-231, U87-MG, MCF-7 and PANC-1), normal MRC-5 fetal fibroblasts, and dental papilla tissue- derived mesenchymal stem cells (DPSCs). Significantly (p<0.05) lower half maximal inhibitory concentration ($IC_{50}$) values were observed in the A-549 and MDA-MB-231 cells showing a high proliferation capacity, compared with other cancer and normal cell lines with a relatively low proliferation capacity. The population doubling time (PDT) was significantly (p<0.05) higher in the $10{\mu}M$ PGG-treated cell lines than those of untreated control cell lines. The present study demonstrated that the $IC_{50}$ value increases proportionally to the extending PDT. A high cell number with senescence-associated ${\beta}-galactosidase$ activity was also observed in the $10{\mu}M$ PGG-treated cells compared with those of untreated control cells. Moreover, the level of telomerase activity was significantly (p<0.05) decreased with $10{\mu}M$ PGG treatment, especially in A-549 and MDA-MB-231 cells showing a high proliferation capacity. Based on these observations, PGG could serve as a potent agent for cancer chemotherapy, as its treatment was more effective in cells with a high proliferation capacity.

• Clinical and Experimental Pediatrics
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• v.48 no.7
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• pp.779-788
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• 2005

Purpose : Echinacea, a traditional plant medicine has been used as immune-stimulant. Recent studies have revealed that extract of Echinacea has immunostimulatory effects on human blood mononuclear cells. This study was designed for the purpose of screening the genes associated with immunologic effects of Echinacea on monocytes and dendritic cells using a cDNA microarray chip. Methods : $CD14^+$ monocyte cells were cultured for one day with Echinacea extract(final concentration : $50{\mu}g/mL$) in experiment 1, but were cultured without Echinacea in experiment 2. The gene expression of these cultured monocytes was analyzed using the cDNA microarray chip. Dendritic cells produced from $CD14^+$ monocyte were cultured for five days with GM-CSF and IL-4, and then cultured for one day with Echinacea in experiment 3, but were done without Echinacea in experiment 4. Results : In experiments 1 and 2, there were 17 significantly expressed genes with average expression ratios above 2.5, including interferon gamma-inducible protein 30(IFI 30), CDC(cell-division-cylcle)-like kinase 2(CLK 2), syndecan binding protein(syntenin), superoxide dismutase 2, etc. In experiments 3 and 4, there were 24 gene, with significantly expressed genes were 24 genes, which were insulin-like growth factor 2(somatomedin A), methyl-CpG binding domain protein 3, IFI 30, small inducible cytokine subfamily A, member 22, etc. The genes encoding CD44, IFI 30, mannose receptor C type 1(MRC 1), chemokine receptor 7(CCR 7), CLK 2, syntenin and cytochrome C oxidase subunit VIII were significantly expressed in both monocytes and dendritic cells cultured with Echinacea. Conclusion : This study employed a cDNA microarray chip to elicit the immune-associated gene profile; the expression was enhanced by Echinacea in CD14+ monocytes and dendritic cells. Thus we laid the basis for the quantitative and functional analysis of genes induced by Echinacea in monocytes and monocyte-derived dendritic cells.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39A no.9
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• pp.535-547
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• 2014

We investigate BER (Bit Error Ratio) performance according to the gain of spatial and frequency diversities in uplink SC-FDMA of SIMO (Single Input Multiple Output) systems. The main results of the analysis in this paper are as follows. First, we prove that performance of integrated system for considering spatial and frequency diversity combining in parallel is equivalent with the performance of sequential system for performing diversity combining in sequence. By signal modeling, it is demonstrated that the performances of both systems are the same when the frequency diversity combining technique of the sequential system is equal to diversity combining technique of the integrated system, and spatial diversity combining technique of the sequential system is performed as MRC in advance of frequency diversity combining. Secondly, it is found that effect on the BER performance is different according to the gain of spatial and frequency diversities, respectively. The frequency diversity gain increases by increasing the number of subcarrier. It might affect the performance improvement of high SNR(Signal to Noise Ratio) while it maintains gap between performances of ZF(Zero Forcing) and MMSE(Minimum Mean Square Error) in frequency diversity combining schemes. Also, spatial diversity gain increases as the number of receiving antennas increases. It means that it can reduce performance gap between ZF and MMSE in frequency diversity combining schemes by increasing the number of receiving antennas. In addition, it might affect the performance improvement of the whole SNR. Finally, through the analysis of performance according to the spatial diversity gain, the performance of ZF in frequency diversity combining is equal to the MMSE if the number of receiving antennas is 6 or more.