• The Korean Journal of Zoology
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• v.17 no.3
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• pp.107-116
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• 1974

The metabolism of CdR-2-$^14 C$ and UdR-2-$^14 C$ in mouse small intestine has been studied in connection with the effect of heat treatment on the enzymes concerned in vitro. CdR-2-$^14 C$ is deaminated reaidly by CdR-aminohydrolase at nucleoside level and then degraded into U by the action of nucleosidase which is quite resistant to cleave N-pentose bond of cytosine nucleosides, CdR and CR. High inactivation temperature of $80^\\circC$ was observed for CdR-aminohydrolase, while nucleosidase has an inactivation temperature of $60^\\circC$. CdR-aminohydrolases in various tissues of mouse were inactivated at $80^\\circC$, but not one in tissues of rabbit. It might be assumed that there are correlations between order specificity and inactivation temperature of the enzyme. A physiological significance of the appearance of CdR-aminohydrolase in differentiated tissues of mammals possibly be regarded as a main function in catabolic pathways.

• Journal of Bio-Environment Control
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• v.9 no.4
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• pp.237-243
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• 2000

Agricultural machine is currently operated by man power in the greenhouse, which is oppressively hot and humid, and is for a farmer not to work in comfortable circumstances. In the future, agricultural machine will not have to operate by man power, but it will need do by unmanned power. In order to put into the automatic and unmanned operation of agricultural machine, this system was designed and built to move through the fixed path in the greenhouse. This system was composed of guiders(wires), a limit switch, an operating equipment, its software for automatizing a machine in the greenhouse. The guider was connected between the wall pillars, and the equipment was able to slide over the fixed path made of the guider, by rectilinear and rotational motion. A micro mouse was developed with a stepping motor to calculate on the success rate of its operation with the system As might be expected, this system with the micro mouse was moved the moved the paths with a success rate of 100% on the flat plane surface in our laboratory. However, on the sand plane or the other materials plane, the success rate was not better than 80%. If the micro mouse were well operated, the success rate was would be 100%. Based on the results of this research, this system would be expected to operate well on the path made of a simple wire.

• The Journal of Korean Medicine
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• v.30 no.5
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• pp.61-76
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• 2009

Background: Membranous nephropathy (MN) is the most common cause of adult nephrotic syndrome worldwide and has been defined as granular subepithelial deposition of immune complexes along the glomerular basement membrane (GBM). MN has few known treatments and gives rise to side effects under treatment with steroids and immunosuppressives. Objective: The purpose of this experimental study was to demonstrate the effects of Scutellariae Radix extract (SRE) treatment on MN mouse model induced by cBSA. Methods: We divided mice into 4 groups. The Normal group had no treatment. We induced MN mouse model to the other 3 groups by injecting cBSA into the abdominal cavity. The control group was treated with cBSA (10 mg/kg, i.p.) only. The second group, 'SRE-250', was treated with cBSA (10 mg/kg, i.p.) and SRE (250 mg/kg, p.o.). The third group, 'SRE-500', was treated with cBSA (10 mg/kg, i.p.) and SRE (500 mg/kg, p.o.). After cBSA and SRE treatment for 4 weeks, gain in body weight, 24hrs proteinuria, serum albumin, total cholesterol, triglyceride, BUN and creatinine of all groups were measured. TNF-$\alpha$, IL-6, IL-1$\beta$, IL-10, IFN-$\gamma$, IgA, IgM and IgG levels of all groups were gauged. H&E staining and electron microscopy of the kidney were observed. Results: SRE showed significant decrease in the 24hrs proteinuria, serum triglyceride, BUN, TNF-$\alpha$, IL-6, serum IgA, IgM and IgG levels compared with the control group. SRE showed increase in the serum IL-10 and IFN-$\gamma$ levels compared with control on RT-PCR. SRE considerably decreased in the thickening of the GBM on H&E staining and deposition of electron-density on electron microscopy of the kidney compared with the control. Conclusions: According to the above results, it is suggested that SRE decreases the symptoms of MN induced by cBSA in mouse model. Therefore, SRE seems to be applicable to MN in clinical practice.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.102-102
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• 2002

Heparin-bindin epidermal growth factor (HB-EGF) is one of the EGF family to be expressed at the time of implantation in the mouse uterus. Although HB-EGF has been shown to stimulate the development of embryo and uterus in the mouse, its correlation between cell adhesion molecules remains undefined. Integrin $\alpha$$_{ν}$$\beta$$_3$, one of the cell adhesion molecules, is an important mediator of cell-substratum and cell-cell adhesion in implantation. In the present studies, we investigated the effects of HB-EGF on the embryonic development, initiation of implantation and expression of integrin $\alpha$$_{ν}$$\beta$$_3$ in in vitro culture, blocking of HB-EGF, RT-PCR and immunofluores cence analysis. The results showed that HB-EGF significantly improved the developmental rate of hatched embryos (24.1%, p<0.01) and outgrowth embryos (42.5%, p<0.01). On the other hand, this growth factor showed no offset before the hatching embryonic stage. Analysis of RT-PCR showed that HB-EGF upregulated the expression level of integrina $\alpha$$_{ν}$$\beta$$_3$ subunit genes on the preimplantation embryo and outgrowth of blastocyst (120hr and 144hr after hCG injection). Immunofluorescence analysis showed that the integrin $\alpha$$_{ν}$$\beta$$_3$ subunits localized at the pericellular borders and cell-cell contact areas. Increase in fluorescence intensity was observed in the HB-EGF treated embryos. Intrauterine injection of an anti-HB-EGF antiserum at day 3 significantly decreased the number of implantation sites (14.4, p<0.01) and significantly increased the number of recovered embryos(6.4, p<0.05) at day 5. From these results, it imply that HB-EGF improve the embryo development and accelerated the expression of integrin $\alpha$$_{ν}$$\beta$$_3$ in the preimplantation mouse embryos.

• Journal of the Korean Institute of Intelligent Systems
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• v.12 no.2
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• pp.97-102
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• 2002

In this paper, we propose the technique for recognizing the human s emotion by using the CCD color image. To do this, we first get the face image by using skin-color from the original color image acquired by the CCD camera. And we propose the method for finding man s feature points(eyebrows, eye, nose, mouse) from the face image and the geometrical method for recognizing human s emotion (surprise, anger, happiness, sadness) from the structural correlation of man s feature feints. The proposed method in this paper recognize the human s emotion by learning the neural network. Finally, we have proven the effectiveness of the Proposed method through the experimentation.

• Journal of the Korean Institute of Intelligent Systems
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• v.14 no.3
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• pp.330-335
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• 2004

In this paper, we propose the technique for recognizing the human emotion by using the color image. To do so, we first extract the skin color region from the color image by using HSI model. Second, we extract the face region from the color image by using Eigenface technique. Third, we find the man's feature points(eyebrows, eye, nose, mouse) from the face image and make the fuzzy model for recognizing the human emotions (surprise, anger, happiness, sadness) from the structural correlation of man's feature points. And then, we infer the human emotion from the fuzzy model. Finally, we have proven the effectiveness of the proposed method through the experimentation.

• The Journal of Dong Guk Oriental Medicine
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• v.7 no.2
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• pp.97-105
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• 1999

Hepatic tissues of ICR mouse were intraperitoneally injeced with Colpomenia bullosa(CB) Extract after Triton WR-1339(TX) injection were observed to investigate the antihyperlipermic effect of CB extract for hyperlipidemic hepatic tissue caused by destruction of lipid metabolism. The hepatic tissues were obtained at hour-24, 48, and 72 after TX injection with CB extract treatment. And then these specimen were fixed in 10% neutral buffer solution and were cryocut. The tissue stained by H&E for general morphology and sudan black B for lipid distribution. The increase of hepatocyte havinig meshlike cytoplasm were shown in all hepatic lobules after TX injection and the hepatic plates were disappeared in the region of meshlike hepatocyte aggregation. But the hepatocyte having meshlike cytoplasm were disappeared and hapatic plate were rearranged in CB extract injected mouse. The number of blue black colored lipid drop in hepatic cytoplasm of mouse injected with TX were increased and the size of lipid drop were enlarged. But the number of lipid drop in hepatic cytoplasm of mouse treated CB extract were decreased and the size of lipid drop were diminished. As results indicated that the accumulation of lipid drop caused by TX injection were mitigated by the antihyperlipidermic effect of CB extract.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2001.12a
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• pp.203-207
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• 2001

In this Paper, we propose the neural network based emotion recognition method for intelligently recognizing the human's emotion using CCD color image. To do this, we first acquire the color image from the CCD camera, and then propose the method for recognizing the expression to be represented the structural correlation of man's feature Points(eyebrows, eye, nose, mouse) It is central technology that the Process of extract, separate and recognize correct data in the image. for representation is expressed by structural corelation of human's feature Points In the Proposed method, human's emotion is divided into four emotion (surprise, anger, happiness, sadness). Had separated complexion area using color-difference of color space by method that have separated background and human's face toughly to change such as external illumination in this paper. For this, we propose an algorithm to extract four feature Points from the face image acquired by the color CCD camera and find normalization face picture and some feature vectors from those. And then we apply back-prapagation algorithm to the secondary feature vector. Finally, we show the Practical application possibility of the proposed method.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2010.07a
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• pp.205-207
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• 2010

본 논문에서는 버블포핑을 구현하는 증강현실 기법을 제안한다. 제안 기법은 진동촉각햅틱 마우스, 비디오 입력용 웹캠, 마커 패턴 영상, 그래픽 구 버블 객체, 그래픽 마우스 등으로 구성된다. 마우스의 이동에 따라 그래픽 마우스가 3차원 공간상에서 이동하면서 버블과의 충돌이 발생하게 되면, 충돌시 발생하는 버블의 이벤트로 fade-out과 diverge and fade-out의 버블 효과를 구현한다. 제안 시스템은 ARToolkit 및 OpenGL을 기반으로 제작되었으며, 시각적인 마커 기반 시스템으로 버블의 움직임과 사용자의 인터랙션을 통해, 버블 포핑의 증강현실을 전달한다.

• BMB Reports
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• v.45 no.1
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• pp.7-13
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• 2012

Phospholipase D (PLD), a superfamily of signaling enzymes that most commonly generate the lipid second messenger Phosphatidic Acid (PA), is found in diverse organisms from bacteria to man and functions in multiple cellular pathways. A fascinating member of the family, MitoPLD, is anchored to the mitochondrial surface and has two reported roles. In the first role, MitoPLD-generated PA regulates mitochondrial shape through facilitating mitochondrial fusion. In the second role, MitoPLD performs a critical function in a pathway that creates a specialized form of RNAi required by developing spermatocytes to suppress transposon mobilization during meiosis. This spermatocyte-specific RNAi, known as piRNA, is generated in the nuage, an electron-dense accumulation of RNA templates and processing proteins that localize adjacent to mitochondria in a structure also called intermitochondrial cement. In this review, we summarize recent findings on these roles for MitoPLD functions, highlighting directions that need to be pursued to define the underlying mechanisms.