• 대한수의학회지
• /
• 제56권4호
• /
• pp.241-247
• /
• 2016

This study was conducted to evaluate the hangover relieving effect of Sanghwang mushroom mycelium extract (SME). The extract showed 1,1-diphenyl-2-picrylhydrazyl radical scavenging effect in a concentration-dependent manner and high antioxidant capacity ($56.67{\pm}1.77%$) when administered at $120{\mu}g/mL$. In addition, SME significantly increased (p < 0.005) the aldehyde dehydronase (ALDH) activity ($126.03{\pm}9.11%$) when applied at 8 or $16{\mu}L/mL$. A locomotor activity test showed that the alcohol-water treated group showed significantly decreased motor activity at 90 min post-administration. However, the alcohol-SME treated group showed a 20-fold higher motor activity than that observed in the alcohol-water treated group at 90 min post-administration. Blood was harvested from each mouse at 90 min post-administration, and both alcohol and aldehyde concentrations were measured. The alcohol-SME treated group showed significantly lower (p < 0.5) alcohol ($120.13{\pm}12.83{\mu}g/mL$) and aldehyde ($7.26{\pm}1.22{\mu}g/mL$) concentrations than the values observed in the alcohol-water treated group. These results suggest that the hangover relieving effect of SME results from increased ALDH activity, which reduces the aldehyde concentration in the blood.

• Journal of Acupuncture Research
• /
• 제18권3호
• /
• pp.1-9
• /
• 2001

Objective : In order to study the effect of contralateral acupuncture on recovery of motor disorders in stroke patients a clinical study was performed. Methods : Thirteen patients with poststroke-hemiplegia were randomized into two groups. Six patients(test group) treated with contralateral acupuncture. The other seven patients(control group) treated with ipsilateral acupuncture. The activity of daily living was measured with a modified barthel index(MBI). The therapy was performed one a day for 3 weeks. Results : In terms of improvement of activity of daily living, the test group showed statistically meningful increase after 1 week treatment. but the control group showed statistically meningful increase after 2 weeks(p<0.05). In terms of score of MBI, the groups showed no statistically meningful difference after 3 week treatment. Conclusions : These results support that contralateral acupuncture therapy has almost same effectness compared with ipsilateral acupuncture therapy in improvement of the activity of daily living of poststroke-hemiplegic patients.

• PNF and Movement
• /
• 제14권3호
• /
• pp.245-254
• /
• 2016

Purpose: This study investigates the influence of elastic resistance exercise using proprioceptive neuromuscular facilitation (PNF) on the daily activities of a patient with incomplete spinal cord injury. The result will be proposed as background data for effective intervention in a patient with incomplete spinal cord injury. Methods: The target subject was a patient with incomplete spinal cord injury to the cervical cord (C6). Elastic resistance exercise based on PNF was performed for 30 min daily, five times a week, for eight weeks. The ASIS motor scale was applied to test the muscular strength of the upper limb, and the spinal cord independence measure II (SCIM II) was used to evaluate the capacity of daily activity. Results: By applying elastic resistance exercise based on PNF, the muscular strength of the upper limb increased and the performance of daily activity improved. Conclusion: Because elastic resistance exercise based on PNF positively influences the ASIA motor scale and SCIM II of the patient with incomplete spinal cord injury. It can be used for training programs to improve the capacity of daily activity of the patient.

• BMB Reports
• /
• 제40권5호
• /
• pp.723-730
• /
• 2007

Kinesin is an ATP-driven microtubule motor protein that plays important roles in control of microtubule dynamics, intracellular transport, cell division and signal transduction. The kinesin superfamily is composed of numerous members that are classified into 14 subfamilies. Animal kinesins have been well characterized. In contrast, plant kinesins have not yet to be characterized adequately. Here, a novel plant-specific kinesin gene, GhKCH2, has been cloned from cotton (Gossypium hirsutum) fibers and biochemically identified by prokaryotic expression, affinity purification, ATPase activity assay and microtubule-binding analysis. The putative motor domain of GhKCH2, $M_{396-734}$ corresponding to amino acids Q396-N734 was fused with 6$\times$His-tag, soluble-expressed in E. coli and affinity-purified in a large amount. The biochemical analysis demonstrated that the basal ATPase activity of $M_{396-734}$ is not activated by $Ca^{2+}$, but stimulated 30-fold max by microtubules. The enzymatic activation is microtubule-concentration-dependent, and the concentration of microtubules that corresponds to half-maximum activation was about 11 ${\mu}M$, much higher than that of other kinesins reported. The cosedimentation assay indicated that $M_{396-734}$ could bind to microtubules in vitro whenever the nucleotide AMP-PNP is present or absent. As a plant-specific microtubule-dependent kinesin with a lower microtubule-affinity and a nucleotide-independent microtubule-binding ability, cotton GhKCH2 might be involved in the function of microtubules during the deposition of cellulose microfibrils in fibers or the formation of cell wall.

• 대한임상검사과학회지
• /
• 제48권1호
• /
• pp.41-48
• /
• 2016

Motor evoked potential of spinal surgery is known to cause damage due to the movement path of the continuous scan operation and surgery can be performed with minimized disability after surgery. However, if it is not at all formed at the wave motion evoked potential can occur during surgery and, in some cases the size of the waveform to be measured is very small and intermittent. In this case, the surgery cannot provide information about whether there is neurological damage. Increased intensity of the wave-induced motion of the dislocation does not occur if it appears in a very small amplitude stimulus, but changing the inspection area that electrical stimulation of the waveform changes could not be found. However, stimulation of a wide area in the cerebral cortex was found to occur with a waveform in the patients who underwent examination. Through this study, we propose a useful motor evoked potential test. From November to December 2015 three spine surgery patients visited Samsung Medical Center as neurosurgery patients with omission discomfort, gait disturbance, and no symptom of strength before surgery. In spine surgery patients with motor grade weakness, when motor evoked potential waveform has not been measured, in examination of the site of electrical stimulation of the cerebral cortex from entering the C3+C5/C4+C6 or C3+C1/C4+C2 if by the activity of more motor neuron unit, it was found that the waveform is better formed.

• 한국콘텐츠학회논문지
• /
• 제9권1호
• /
• pp.348-358
• /
• 2009

미만성 축삭손상(diffuse axonal injury)은 외상성 뇌손상의 일반적인 형태이며, 인지 장애의 주요 원인으로 생각되어 진다. 흔들린 아기 증후군(shaken baby syndrome)과 같이 뇌에 전단력이 심하게 가해졌을 때도 신체 장애 뿐만 아니라 인지 장애가 특징적으로 나타난다. 신체 활동은 건강 증진과 더불어 기억 및 학습과 관련된 해마의 기능 향상에도 영향을 미친다. 본 연구의 목적은 흰쥐를 대상으로 미만성 축삭 손상을 일으킨 후 반복적인 운동 훈련을 통해 운동 수행력을 관찰하고 해마에서 GAP-43의 발현을 통해 축삭 재생의 변화를 관찰하는 것이었다. 실험동물은 운동 훈련을 적용시키는 실험군과 대조군으로 구분하였고, 각각의 군을 다시 1일, 7일 및 14일군으로 구분하였다. 그 결과, 운동 훈련을 적용시킨 실험군이 대조군보다 운동 수행력의 향상이 더 유의했으며, 해마에서 GAP-43의 발현도 같은 양상을 나타냈다. 이러한 차이는 7일군과 14일군보다 1일군과 7일군 사이에 더 크게 나타났다. 그러므로 미만성 축삭손상 후 운동 훈련은 운동 수행력의 향상에 영향을 미치며, 인지와 관련된 해마의 구조적 변화도 야기 시키는 것으로 생각된다.

• 대한물리치료과학회지
• /
• 제8권1호
• /
• pp.821-831
• /
• 2001

Motor ability were hypothesized to be major factors that may have an influence on IADL(instrumental activities of daily living). The purpose of this study was to investi gate the effect of motor ability of elderly on IADL. This study consisted of forty eight elder male and ninety elder female. The subjects were selected from L, S, Y-institution in kyoung ki do. The average age of elder male and female was 73.81, 71.38 years. The data were analyzed with t-test, repeated measurement, correlation test, regression test, using SPSS PC+ and MINITAB program. The measurement item of motor ability was muscle strength(left, right- grip strength), flexibility(sitting trunk flexion, trunk extension, leg raising while in a standing position), agility(whole body reaction test, standing up), power(sargent jump), balance (stork stand). IADL was measured with Philadelphia Geriatric Center IADL. The result of this study were the following : 1. The factor of significant difference of between elder male and female was muscle strength, flexibility, power but no significant difference was agility, balance. 2. Between IADL of elder male and female was no significant difference. 3. Correlation of IADL and Motor ability of elder male had effect on muscle strength and power, agility. Correlation of Body composition and Motor ability of elder female had effect on muscle strength and power, agility. As a result of this study, optimal motor ability of elder male and female had improved IADL. In addition to, this result of this study, it can suggested the consideration of the Health promotion program for elderly.

• 대한물리치료과학회지
• /
• 제19권3호
• /
• pp.23-30
• /
• 2012

The purpose of this study was to apply treadmill training through motor learning to cerebral palsy children and examine its effects on their Gross Motor Functions. The subjects of this study were 13 spastic diplegia children who had difficulty in independent gait, and GMFCS level III, IV. We performed treadmill gait training using the principle of weight bearing, based on 4times a week for 30 minutes before and after each session physical therapy we gave weight bearing treadmill training 5 to 10 minutes, during 7 weeks(April 9, 2012~May 26, 2012) fittingly for the children's gait characteristics. In order to test how the weight bearing treadmill training affects spastic diplegia children's gross motor functions, we measured body mobility with Gross Motor Function Measure (GMFM). These data were collected before and after the experiment and analyzed through comparison. Data collected from the 13 spastic diplegia children the results were as follows. For evaluating with regard to change in body mobility, significant difference was observed between before and after the experiment in measured gross motor functions, which were crawling, kneeling, standing, walking, jumping and running(p<0.05). According to the results of this study, when gait training through motor learning was applied to spastic cerebral palsy children, it made significant changes in their body mobility. Accordingly, for the effective application of gait training through motor learning to cerebral palsy children, it is considered necessary to make research from different angle on how such training affects children's mobility, activity of muscles in the lower limbs, and gait characteristics.

• 대한간호학회지
• /
• 제33권5호
• /
• pp.591-600
• /
• 2003

Purpose: The purpose of this study was to investigate the effects of exercise training using biofeedback and constraint-induced movement on the upper extremity function of hemiplegic patients. Method: A non-equivalent pretest-posttest design was used. Study subjects were a conveniently selected group of 40 hemiplegic patients(20 experimental subjects, 20 control subjects) who have been enrolled in two community health centers. After biofeedback training the subjects of experimental group were given constraint-induced movement, involving restraint of unaffected U/E in a sling for about 6 hours in a day over a period of two weeks, while at the same time intensively training the affected U/E. Outcomes were evaluated on the basis of the VIE motor ability(hand function, grip power, pinch power, U/E ROMs), and motor activity(amount, quality). Result: 1. After 2 weeks of treatment, the motor abilities of affected U/E(hand function, grip power, pinch power, ROMs of wrist flexion, elbow flexion and shoulder flexion/extension) were significantly higher in subjects who participated in exercise training than in subjects in the control group with no decrement at 4-week follow-up. However, there was no significant difference in wrist extension between experimental or control group. 2. After 2 weeks of treatment, the amount of use and the quality of motor activity of affected U/E were significantly higher in subjects who participated in exercise training than in subjects in the control group with no decrement at 4-week follow-up. Conclusion: The above results state that exercise training using biofeedback and constraint-induced movement could be an effective intervention for improving U/E function of chronic hemiplegic patients. Long-term studies are needed to determine the lasting effects of constraint-induced movement.

• 대한한방부인과학회지
• /
• 제27권3호
• /
• pp.1-11
• /
• 2014

Objectives: Hwangryunhaedok-tang (HH) is a representative heat clearing multiherb prescription. We evaluated the protective effect of HH against heat stress exposure in mice. Methods: Six weeks old ICR mice were used for this study. After $43^{\circ}C$ heat stress for 15 minutes, we evaluated the changes of motor activity and c-fos expression level to determine the proper heat stress and evaluation time. The subjects were divided into 4 groups (1. control group, 2. heat stress group with normal saline, 3. heat stress group with administration of 100 mg/kg, and 4. heat stress group with administration of 1000 mg/kg). After oral administration of HH once a day for 3 days, 2, 3, 4 groups were exposed to $43^{\circ}C$ heat stress for 15 minutes. Then, we evaluated the motor activity for 120 minutes and analyzed the c-fos expression using western blot. We investigated the effect of HH and its ingredients on c-fos expressions after heat stress. The mice were administrated HH, Scutellariae Radix, Coptidis Rhizoma, Phellodendri Cortex, and Gardeniae Fructus for 3 days. After one hour of last treatment, the mice were exposed to heat stress at $43^{\circ}C$ for 15 min. After two hours, the hypothalamus was dissociated and lysed to measure c-fos expression. Results: After oral administration of HH for 3 days, motor activity was recovered insignificantly. 100 mg/kg HH treatment reduced the c-fos expression after heat stress but insignificantly. Among the ingredients of HH, Coptidis Rhizoma and Scutellariae Radix treatment groups significantly reduced the c-fos after stress. Conclusions: These results show that Hwangryunhaedok-tang may be effective to reduce the heat stress response.