• 한국의상디자인학회지
• /
• 제13권2호
• /
• pp.47-62
• /
• 2011

The purpose of this study was to investigate the knitwear preferences and purchase behaviors of university students. The subjects were 493 university students in Daejeon and Chungnam Provinces. The method of this study was a survey and measurement instruments were 34 stimuli which were manipulated pullover patterns and shapes and self-administrated questionnaire with knitwear design preference items and knitwear purchase behavior items. Data were analyzed by factor analysis, frequency analysis, Cronbach'${\alpha}$, t-test, ANOVA and $Sch{\acute{e}}ffe$ test using SPSS program. The results of the study were as follows. First, university students most preferred achromatic colors, pastel tones, solid patterns, and 100% cotton. Second, as for knitwear purchase behaviors, university students considered the esthetical factor to be most important among 4 dimensions (comfortable, esthetical, economical, and conspicuous factors) as important purchase criteria, and they used internet web sites for knitwear purchases. Third, there were significant differences in preferred pullover shape depending on neckline shape, neck depth, sleeve shape and clothing length. University students preferred the classic pullover design with V neckline, normal neck depth, set-in sleeves and normal length. Fourth, university students preferred the cloud motif and riding man motif among the 9 Baekje traditional motifs and one point small pattern on the left chest and crosswise bending pattern for pattern arrangement.

• BMB Reports
• /
• 제37권2호
• /
• pp.254-259
• /
• 2004

The wild-type and temperature-sensitive (ts) repressor genes were cloned from the temperate mycobacteriophage L1 and its mutant L1cIts391, respectively. A sequencing analysis revealed that the $131^{st}$ proline residue of the wild-type repressor was changed to leucine in the ts mutant repressor. The 100% identity that was discovered between the two DNA regions of phages L1 and L5, carrying the same sets of genes including their repressor genes, strengthened the speculation that L1 is a minor variant of phage L5 or vice versa. A comparative analysis of the repressor proteins of different mycobacteriophages suggests that the mycobacteriophage-specific repressor proteins constitute a new family of repressors, which were possibly evolved from a common ancestor. Alignment of the mycobacteriophage-specific repressor proteins showed at least 7 blocks (designated I-VII) that carried 3-8 identical amino acid residues. The amino acid residues of blocks V, VI, and some residues downstream to block VI are crucial for the function of the L1 (or L5) repressor. Blocks I and II possibly form the turn and helix 2 regions of the HTH motif of the repressor. Block IV in the L1 repressor is part of the most charged region encompassing amino acid residues 72-92, which flanks the putative N-terminal basic (residues 1-71) and C-terminal acidic (residues 93-183) domains of L1 repressor.

• 대한지리학회지
• /
• 제44권2호
• /
• pp.176-186
• /
• 2009

경제적, 환경적 이유에서, 승용차 이용을 억제하고 대중교통수단 분담율을 제고하는 교통정책은 수도권 지자체에게 있어 매우 중요한 과제이다. 통행행태를 근본적으로 이해하는 것은 이러한 정책의 수립과 집행에 매우 중요한 필요조건이다. 소규모 샘플자료를 이용, 특정한 통행 행태와 몇몇의 사회경제적 혹은 지리공간적 변수들 간의 상관관계에 대한 횡단면적 분석을 행하는 것이 이 분야 전통적인 연구주제였다. 연구결과는 스냅샷과 유사한, 시공간적으로 제한된 정보만을 제공한다. 그러나, 통행은 공간적으로 상이한 장소에서 벌어지는 일상활동 참여로부터 파생되며, 일상 활동계획 상에서의 활동-통행 간 순서 관계는 통행 행태의 중요한 틀이다. 본 연구는 다차원 정보배열비교법과 핵심정보배열추출기법을 이용, 서울시민의 일일 대중교통 이용 자료인 스마트 카드 자료를 분석하여 서울시내 대중교통 이용 행태의 일반 특성을 탐구한다. 분석 결과 서울 강남/북 간 버스-전철 연계통행과 간선버스 통행간 통행 행태의 중요한 차이를 확인할 수 있었다. 즉 통행패턴은 보통 강북과 강남 권역 안에서 형성이 되어 있어, 자연 장애물인 한강을 건너 강남/북을 가로지르는 통행패턴은 상대적으로 적었다. 또한 강북과 강남 각각의 권역 안에서 CBD를 들르는 통행패턴은 보통 전철 혹은 지선버스-전철의 연계교통수단을 이용하는 데 반해 지선버스 혹은 간선버스만을 이용하는 통행패턴은 CBD가 아닌 지역으로 목적지가 국한되는 경향이 있다.

• Applied Biological Chemistry
• /
• 제38권1호
• /
• pp.49-54
• /
• 1995

한국 마늘에 감염된 바이러스의 종류와 병 발생 메카니즘을 구명하기 위하여, 마늘 바이러스 cDNA clone들을 분리하였다. 24개 cDNA clone들의 부분적인 염기 서열을 결정하였고, 이 중 poly(A) tail을 가진 5개 clone들의 염기 서열을 결정하였다. 이를 이미 알려진 다른 식물 바이러스와 비교했을 때, clone V9은 일차구조가 carlavirus와 유사성을 보이므로 GLV cDNA clone으로 여겨진다. Northern blot 결과로부터 GLV genome의 크기는 8.5 knt이고, poly(A) tail을 가지고 있다는 것을 알 수 있었다. clone V9의 3' 말단부분에는 바이러스 복제과정에서 cis-acting element로 작용한다고 여겨지는 hexanucleotide motif(5'-ACCUAA)가 존재한다. 또한 carlavirus의 껍질 단백질 subgenomic RNA의 5' 말단에 보존되어 있는 5'-TTAGGT도 나타난다. 이들은 모두 carlavirus의 특징들이다. 껍질 단백질 유전자를 pRSET-A 발현 벡터에 재조합하고, E. coli BL21에서 발현시켰다. 발현된 껍질 단백질을 $Ni^{2+}$ NTA affinity chromatography에 의해 정제하였다. 껍질 단백질을 토끼에 주사하여 항체를 만든 후, immunoblot을 한 결과 GLV 껍질 단백질에 해당하는 24 kDa polypeptide가 인지되었다. 또한 다양한 마늘 품종에 대해서 immunoblot을 한 결과, GLV 껍질 단백질의 크기와 GLV의 감염정도가 마늘 품종에 따라서 차이가 있다는 것을 알 수 있었다.

• The Plant Pathology Journal
• /
• 제22권3호
• /
• pp.260-264
• /
• 2006

Talaromyces flavus mediates the transmission of Apple stem grooving virus (ASGV) to several host plants. The ASGV-F carried by T.flavus was partially purified from the fungus. Based on sequence analysis and homology searches, this is closely related to other ASGV strains isolated from host plants. The partially purified viral coat protein (CP) was separated on a 12% SDS-polyacrylamide gel and analyzed by Western blotting with an ASGV anti-serum. A single band at 28 kDa reacted with the ASGV anti-serum. The deduced amino acid sequence of the ORF-l showed conserved domains, including an NTP-binding helicase motif, GFAGSGKT. The amino acid sequences of the helicase and CP showed strong homology to other ASGV strains (98%). All ASGV isolated from plants and fungi had salt bridges composed of the CP and the GFAGSGKT motif of the helicase, which are commonly conserved in plant viruses. These results suggest that ASGV-F is one of ASGV strains isolated from T.flavus based on sequence similarity as well as the serological analysis of CP.

• 한국환경성돌연변이발암원학회지
• /
• 제22권3호
• /
• pp.137-141
• /
• 2002

본 연구는 분열형 효모 Schizosaccharomyces pombe에서 여러 가지 DNA 절제회복 및 유전자 발현에 관여하는 SNF2/SW12유전자의 기능을 연구하기 위하여 이에 관련되는 유전자를 분리하고 그 특성을 연구하였다. SNF2 motif 의 conserved sequence를 primer로 하여 중합효소 연쇄반응(PCR) 방법으로 480 bp 크기의 DNA fragment를 분리하여, 이를 probe로 하여 효모에서 hrp2＋ 유전자를 분리하였다. 분리한 hrp2＋ 유전자의 sequence homology를 비교한 결과 3개의 SNF2 motif를 포함하고 있었다. hrp2＋유전자의 전사체 크기는 4.7 kb임을 Northern hybridization으로 확인하였다. hrp2＋유전자의 전사 개시 부위를 알기 위하여 primer extension분석을 한 결과, 첫 번째 ATG에서 약47 base pair 위쪽에 위치함을 확인하였다. 또한 특성 연구를 위하여 Northern hybridization으로 hrp2＋ 유전자의 UV와 MMS에 대한 유도성을 조사한 결과 자외선에 대해서만 유전자의 발현이 유도되었다. 이 결과 분리한 hrp2＋는 UV-inducible 유전자임을 확인하였다.

• Genomics & Informatics
• /
• 제4권4호
• /
• pp.147-160
• /
• 2006

GATA transcription factors are widespread eukaryotic regulators whose DNA-binding domain is a class IV zinc finger motif in the form $CX_{2}CX_{17-20}CX_{2}C$followed by a basic region. In fungi, they act as transcriptional activators or repressors in several different processes, ranging from nitrogen source utilization to mating-type switching. Using an in-house bioinformatics portal system, we surveyed 50 fungal and 9 out-group genomes and identified 396 putative fungal GATA transcription factors. The proportion of GATA transcription factors within a genome varied among taxonomic lineages. Subsequent analyses of phylogenetic relationships among the fungal GATA transcription factors, as well as a study of their domain architecture and gene structure, demonstrated high degrees of conservation in type IVa and type IVb zinc finger motifs and the existence of distinctive clusters at least at the level of subphylum. The SFH1 subgroup with a 20-residue loop was newly identified, in addition to six well-defined subgroups in the subphylum Pezizomycotina. Furthermore, a novel GATA motif with a 2f-residue loop ($CX_{2}CX_{21}CX_{2}C$, designated 'zinc finger type IVc') was discovered within the phylum Basidiomycota. Our results suggest that fungal GATA factors might have undergone multiple distinct modes of evolution resulting in diversified cellular modulation in fungi.

• Genomics & Informatics
• /
• 제3권4호
• /
• pp.142-148
• /
• 2005

The immune response-related genes have been suggested to be the most favorable genes for positive selection during evolution. Comparing the entire DNA sequence of chimpanzee chromosome 22 (PTR22) with human chromosome 21 (HSA21), we have identified 15 orthologs having indel in their coding sequences. Among them, interferon-${\alpha}$ receptor-1 gene (IFNAR1), an immuneresponse-related gene, is subjected to comparative genomic analysis. Chimpanzee IFNAR1 showed the same genomic structure as human IFNAR1 (11 exons and 10 introns) except the 3 bp insertion in exon 4. The sequence alignment of IFNAR1 coding sequence indicated that 'ISPP' amino acid sequence motif is highly conserved in chimpanzee and other animals including mouse and chicken. However, the human IFNAR1 shows that one proline residue is missing in the sequence motif. The homology modeling of the IFNAR1 structures suggests that the proline deletion in human IFNAR1 leads to the formation of the following ${\alpha}$-helix, whereas two sequential prolines in chimpanzee IFNAR1 inhibit it. As a result, human IFNAR1 may adopt a characteristic structure distinct from chimpanzee IFNAR1. This human specific trait could contribute to specific immune response in the most optimized manner for humans. Further molecular biological studies on the IFNAR1 will help us to gain insights into the molecular implication of species-specific host-pathogen interaction in primate evolution.

• 패션비즈니스
• /
• 제16권2호
• /
• pp.44-61
• /
• 2012

The 21st century directs designomics to achieve wealth by designing old beyond a simple recognition of the importance of culture. One of the representative local cultures of Gyeongnam is Gaya culture. The Gaya period had over 600 years history with rich and excellent iron manufacturing skill and advanced pottery culture. It is the very time to reevaluate the importance of Gaya equivalent to the Three Kingdoms in our ancient history which was has been so far ignored and isolated. In order to develop unique and excellent culture of Gaya as local fashion cutural goods, questionnaire survey on the recognition of Gaya culture and cultural goods purchasing status had been conducted to target visitors of Representative National Museum. Data Analysis Using SPSS 18.0 Win was performed with frequency analysis and t-test. As to the questionnaire survey, important criteria for purchasing in case of buying cultural goods could be found and the residents in Gyeongnam had higher recognition of developing cultural goods by the motif of Gaya relics. 'Mounted vessel in the shape of warrior on horseback(12.4%)' and 'Chariot wheel-shaped Pottery(10.8%)' were selected the most suitable motif, 'Cellphone strap(11.0%)' and 'key holder(10.2%), 'cup (7.2%)'were selected the most suitable item of cutural goods. These will be actively reflected in the development of fashion cultural goods design applying Gaya relics.

• 방사선산업학회지
• /
• 제2권3호
• /
• pp.97-104
• /
• 2008

Cinnamate-4-hydroxylase (C4H) is a key enzyme of phenylpropanoid pathway, which leads a variety of secondary metabolites to participate in differentiation and protection of plant against environmental stresses. In this study, we isolated a full-length cDNA of the C4H gene from a black raspberry (Rubus occidentalis L.), using a reverse transcriptase-PCR and rapid amplification of the cDNA ends (RACE)-PCR. The full-length cDNA of the RocC4H gene contained a 1,515 bp open reading frame (ORF) encoding a 504 amino acid protein with a calculated molecular weight of about 57.9 kDa and an isoelectric point (pI) value of 9.1. The genomic DNA analysis revealed that RocC4H gene had three exons and two introns. By multiple sequence alignment, RocC4H protein was highly homologous with other plant C4Hs, and the cytochrome P450-featured motifs, such as the heme-binding domain, the T-containing binding pocket motif (AAIETT), the ERR triad, and the tetrapeptide (PPGP) hinge motif, were highly conserved. Southern blot analysis revealed that RocC4H is a single copy gene in R. occidentalis.