• Title/Summary/Keyword: morphometric technique

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Morphometrical Analysis of Developing Renal Tubular and Glomerular Cells in Rabbit (토끼의 발생기 뇨세관과 사구체 세포들의 형태계측학적인 분석)

  • 정호중;양영철;배기원
    • Journal of Life Science
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    • v.8 no.4
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    • pp.449-456
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    • 1998
  • Using morphometric technique, the present study was made of the kidney of rabbit embryo in the 13 days of the gestation. Their section areas of the renal tubules and the glomerular cells were compared with the those of adult rabbit, 1. In the developing renal tubule, the tubular section areas were larger than that of the adult, but the nuclear section areas of tubular calls were smaller than the adult. The microvilli of their cell surface were poorly developed, and their cytoplasm contained well developed mitochondria with euchromatic nucleus. 2. In the developing glomeruli. the nuclear section area of the cells of the parietal layer, the podocytes, and the endothelial cells were similar size, but their nuclei were larger than those of the adult. The cytopalsm of the podocytes contained well-developed rough endoplasmic reticulum, Golgi apparatus, and round mitochondria.

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Morphometric and Genetic Variability Among Tylenchulus semipenetrans Populations from Citrus Growing Area in Korea

  • Park, Byeong-Yong;Park, Sun-Nam;Lee, Jae-Kook;Bae, Chang-Hwan
    • The Plant Pathology Journal
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    • v.25 no.3
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    • pp.236-240
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    • 2009
  • Tylenchulus semipenetrans, citrus nematode is an important phytopathogenic nematode and responsible for serious damage on citrus. However, little information is available about genetic variability of T. semipenetrans among different populations with variation of conventional diagnostic characteristics. In this study, we compared the morphometric and genetic characteristics among different populations. The mature female of T. semipenetrans collected in this study had thicker cuticle than those in the previous studies. In comparative sequence analysis of T. semipenetrans populations obtained from Jeju in Korea, we observed genetic variations within clones generated from single individuals. To determine whether variability among copies of nuclear ribosomal DNA sequences exists in the genome of T. semipenetrans, PCR-RFLP technique from individuals of Korean isolates with MseI and MspI restriction enzymes was used to prove experimentally that all populations have intra-specific variations. Restriction enzyme digestion created several fragments on 3.0% agarose gel corresponding to several haplotypes in all populations, though some populations displayed fragment deletion. The total length of fragments was larger than before digestion, indicating sequence heterogeneity within the genome of T. semipenetrans.

Linear-Time Korean Morphological Analysis Using an Action-based Local Monotonic Attention Mechanism

  • Hwang, Hyunsun;Lee, Changki
    • ETRI Journal
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    • v.42 no.1
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    • pp.101-107
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    • 2020
  • For Korean language processing, morphological analysis is a critical component that requires extensive work. This morphological analysis can be conducted in an end-to-end manner without requiring a complicated feature design using a sequence-to-sequence model. However, the sequence-to-sequence model has a time complexity of O(n2) for an input length n when using the attention mechanism technique for high performance. In this study, we propose a linear-time Korean morphological analysis model using a local monotonic attention mechanism relying on monotonic alignment, which is a characteristic of Korean morphological analysis. The proposed model indicates an extreme improvement in a single threaded environment and a high morphometric F1-measure even for a hard attention model with the elimination of the attention mechanism formula.

Clinical Outcome of Modified Cervical Lateral Mass Screw Fixation Technique

  • Kim, Seong-Hwan;Seo, Won-Deog;Kim, Ki-Hong;Yeo, Hyung-Tae;Choi, Gi-Hwan;Kim, Dae-Hyun
    • Journal of Korean Neurosurgical Society
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    • v.52 no.2
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    • pp.114-119
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    • 2012
  • Objective : The purpose of this study was 1) to analyze clinically-executed cervical lateral mass screw fixation by the Kim's technique as suggested in the previous morphometric and cadaveric study and 2) to examine various complications and bicortical purchase that are important for b-one fusion. Methods : A retrospective study was done on the charts, operative records, radiographs, and clinical follow up of thirty-nine patients. One hundred and seventy-eight lateral mass screws were analyzed. The spinal nerve injury, violation of the facet joint, vertebral artery injury, and the bicortical purchases were examined at each lateral mass. Results : All thirty-nine patients received instrumentations with poly axial screws and rod systems, in which one hundred and seventy-eight screws in total. No vertebral artery injury or nerve root injury were observed. Sixteen facet joint violations were observed (9.0%). Bicortical purchases were achieved on one hundred and fifty-six (87.6%). Bone fusion was achieved in all patients. Conclusion : The advantages of the Kim's technique are that it is performed by using given anatomical structures and that the complication rate is as low as those of other known techniques. The Kim's technique can be performed easily and safely without fluoroscopic assistance for the treatment of many cervical diseases.

Comparative Analysis of Cervical Lateral Mass Screw Insertion among Three Techniques in the Korean Population by Quantitative Measurements with Reformatted 2D CT Scan Images : Clinical Research

  • Cho, Jae-Ik;Kim, Dae-Hyun
    • Journal of Korean Neurosurgical Society
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    • v.44 no.3
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    • pp.124-130
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    • 2008
  • Objective : Our purpose of this study is to compare insertion angles and screw lengths from Roy-Camille, Magerl, and our designed method for cervical lateral mass screw fixation in the Korean population by quantitative measurement of reformatted two dimensional (2D) computed tomography (CT) images. Methods : We selected thirty Korean patients who were evaluated with thin section CT scans and reconstruction program to obtain reformatted 2D-CT images of the transversal plane passing the cranio-caudal angle using three different techniques. We measured the minimum angle to avoid vertebral artery (VA) injury, the ideal angle and depth for bicortical screwing of cervical lateral mass. Morphometric measurements of the lateral masses from C3-C7 were also taken. Results : In all three techniques, the mean safety angles from the VA were less than 8 degrees and the necessary depth of the screw was about 14 mm for safety to the VA and for the bicortical purchase. In our designed technique, the mean $\beta$ angles of each level from C3 to C7 were 29.0. 29.8. 29.5. 26.3. and 23.9 degrees, respectively. Conclusion : Results of this study and data from the literature indicate that differences may exist between the Korean and Western people in the length and angle for ideal lateral mass screw fixation. In addition, our technique needs further cadaveric and clinical study for safety and efficacy for being performed as alternative method for cervical lateral mass fixation.

Application of RTI to Improve Image Clarity of a Trace Fossil Cochlichnus Found from the Jinju and Haman Formations

  • Sangho Won;Dal-Yong Kong
    • Economic and Environmental Geology
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    • v.56 no.4
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    • pp.397-408
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    • 2023
  • A total of 64 specimens of trace fossils were collected from the Jinju Formation of the construction site of Jinju Aviation Industrial Complex, and from the Haman Formation of Namhae Gain-ri fossil site. The fossils are continuously and regularly meandering sine-curve in shape. The fossil varies in morphology: width between 0.2 and 5.6 mm, wavelength between 1.5 and 28 mm, and amplitude between 0.9 and 7.9 mm; the Jinju specimens are commonly wider than the Haman ones. The ratio of wavelength to amplitude is more or less regular regardless of width of the specimen, and the linear correlation of the ratios shows that the Jinju specimens fit better than the Haman specimens. Taking all morphometric parameters, specimens in all size ranges are temporarily identified as ichnospecies Cochlichnus anguineus. In order to obtain more distinct and clearer images of Cochlichnus, we selected two specimens and applied a new imaging technology RTI. For photography of the trace fossils, 50 to 80 images were taken per set with photometric lighting close to the surface and horizontally. RTI technology clearly showed that the images of tiny fossils were improved: the surface contrast become sharper and messy and unnecessary information disappeared. Currently, RTI technology is used in many fields including preservation of cultural properties and archaeology. As a consequence, we hope to apply this technique to the field of paleontology, especially to the study of trace fossils of very small size.

Measurement of Critical Structures around Paraclinoidal Area : A Cadaveric Morphometric Study

  • Lee, Hyun-Woo;Park, Hyun-Seok;Yoo, Ki-Soo;Kim, Ki-Uk;Song, Young-Jin
    • Journal of Korean Neurosurgical Society
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    • v.54 no.1
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    • pp.14-18
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    • 2013
  • Objective : Although removal of the anterior clinoid process (ACP) is essential surgical technique, studies about quantitative measurements of the space broadening by the anterior clinoidectomy are rare. The purposes of this study are to investigate the dimension of the ACP, to quantify the improved exposure of the parasellar space after extradural anterior clinoidectomy and to measure the correlation of each structure around the paraclinoidal area. Methods : Eleven formalin-fixed Korean adult cadaveric heads were used and frontotemporal craniotomies were done bilaterally. The length of C6 segment of the internal carotid artery on its lateral and medial side and optic nerve length were checked before and after anterior clinoidectomy. The basal width and height of the ACP were measured. The relationships among the paraclinoidal structures were assessed. The origin and projection of the ophthalmic artery (OA) were investigated. Results : The mean values of intradural basal width and height of the ACP were 10.82 mm and 7.61 mm respectively. The mean length of the C6 lateral and medial side increased 49%. The mean length of optic nerve increased 97%. At the parasellar area, the lengths from the optic strut to the falciform liament, distal dural ring, origin of OA were 6.69 mm, 9.36 mm and 5.99 mm, respectively. The distance between CN III and IV was 11.06 mm. Conclusion : With the removal of ACP, exposure of the C6 segments and optic nerve can expand 49% and 97%, respectively. This technique should be among a surgeon's essential skills for treating lesions around the parasellar area.

Detecting Malignant Urothelial Cells by Morphometric Analysis of $ThinPrep^{(R)}$ Liquid-based Urine Cytology Specimens (형태 계측학적 분석과 $ThinPrep^{(R)}$ 액상 소변세포검사를 이용한 악성 요로상피 세포 검출)

  • Shin, Bong-Kyung;Lee, Young-Suk;Jeong, Hoi-Seon;Lee, Sang-Ho;Kim, Hyun-Chul;Kim, A-Ree;Kim, In-Sun;Kim, Han-Kyeom
    • The Korean Journal of Cytopathology
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    • v.19 no.2
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    • pp.136-143
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    • 2008
  • Urothelial carcinoma accounts for 90% of all the cases of bladder cancer. Although many cases can be easily managed by local excision, urothelial carcinoma rather frequently recurs, tends to progress to muscle invasion, and requires regular follow-ups. Urine cytology is a main approach for the follow-up of bladder tumors. It is noninvasive, but it has low sensitivity of around 50% with using the conventional cytospin preparation. Liquid-based cytology (LBC) has been developed as a replacement for the conventional technique. We compared the cytomorphometric parameters of $ThinPrep^{(R)}$ and cytospin preparation urine cytology to see whether there are definite differences between the two methods and which technique allows malignant cells to be more effectively discriminated from benign cells. The nuclear-to-cytoplasmic ratio value, as measured by digital image analysis, was efficient for differentiating malignant and benign urothelial cells, and this was irrespective of the preparation method and the tumor grade. Neither the $ThinPrep^{(R)}$ nor the conventional preparation cytology was definitely superior for distinguishing malignant cells from benign cells by cytomorphometric analysis of the adequately preserved cells. However, the $ThinPrep^{(R)}$ preparation showed significant advantages when considering the better preservation and cellularity with a clear background.

In Vitro Cultivation of Cymatocarpus solearis(Brachycoeliidae) Metacercariae to Obtain the Adult Stage without the Marine Turtle Definitive Host

  • Grano-Maldonado, Mayra;Alvarez-Cadena, Jose
    • Parasites, Hosts and Diseases
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    • v.48 no.1
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    • pp.49-55
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    • 2010
  • In vitro cultivation of trematodes would assist studies on the basic biology of the parasites and their hosts. This is the first study to use the yolk of unfertilized chicken eggs as a simple and successful method of ovocultivation and the first time to obtain the adult-stage of the trematode Cymatocarpus solearis Braun, 1899 (Digenea: Brachycoeliidae). Chicken eggs were inoculated with metacercariae from the muscle of the spiny lobster, Panulirus argus (Latreille, 1804). The metacercariae were excysted and incubated for 576 hr (24 days) at $38^{\circ}C$ to obtain the adult stage. Eggs in utero were normal in shape and light brown color. The metacercariae developed into mature parasites that have been identified as the adultstage found in marine turtles. The adult lobsters collected in Quintana Roo State, Mexico, showed the prevalence of 49.4% and the mean intensity of 26.0 per host (n=87). A statistical study was performed to determine that no parasitic preference was detected for male versus female parasitized lobsters. Morphometric measurements of the adult-stage of C. solearis obtained in our study have been deposited in the National Helminths Collection of the Institute of Biology of the National Autonomous University of Mexico. This study is significant because it is the first time that a digenean of the family Brachycoeliidae has been demonstrated to develop in vitro from metacercariae into adults capable of producing eggs using the yolk of unfertilized chicken eggs. Secondly, this technique allows to obtain the adult stage of C. solearis without the presence of its marine turtle host, allows us to describe the mature parasites, and thus contribute to our understanding of the biology of C. solearis.

Can a spontaneous smile invalidate facial identification by photo-anthropometry?

  • Pinto, Paulo Henrique Viana;Rodrigues, Caio Henrique Pinke;Rozatto, Juliana Rodrigues;da Silva, Ana Maria Bettoni Rodrigues;Bruni, Aline Thais;da Silva, Marco Antonio Moreira Rodrigues;da Silva, Ricardo Henrique Alves
    • Imaging Science in Dentistry
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    • v.51 no.3
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    • pp.279-290
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    • 2021
  • Purpose: Using images in the facial image comparison process poses a challenge for forensic experts due to limitations such as the presence of facial expressions. The aims of this study were to analyze how morphometric changes in the face during a spontaneous smile influence the facial image comparison process and to evaluate the reproducibility of measurements obtained by digital stereophotogrammetry in these situations. Materials and Methods: Three examiners used digital stereophotogrammetry to obtain 3-dimensional images of the faces of 10 female participants(aged between 23 and 45 years). Photographs of the participants' faces were captured with their faces at rest (group 1) and with a spontaneous smile (group 2), resulting in a total of 60 3-dimensional images. The digital stereophotogrammetry device obtained the images with a 3.5-ms capture time, which prevented undesirable movements of the participants. Linear measurements between facial landmarks were made, in units of millimeters, and the data were subjected to multivariate and univariate statistical analyses using Pirouette® version 4.5 (InfoMetrix Inc., Woodinville, WA, USA) and Microsoft Excel® (Microsoft Corp., Redmond, WA, USA), respectively. Results: The measurements that most strongly influenced the separation of the groups were related to the labial/buccal region. In general, the data showed low standard deviations, which differed by less than 10% from the measured mean values, demonstrating that the digital stereophotogrammetry technique was reproducible. Conclusion: The impact of spontaneous smiles on the facial image comparison process should be considered, and digital stereophotogrammetry provided good reproducibility.