The Korean Journal of Cytopathology (대한세포병리학회지)

The Korean Society for Cytopathology (대한세포병리학회)
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Detecting Malignant Urothelial Cells by Morphometric Analysis of $ThinPrep^{(R)}$ Liquid-based Urine Cytology Specimens

형태 계측학적 분석과 $ThinPrep^{(R)}$ 액상 소변세포검사를 이용한 악성 요로상피 세포 검출

  • Shin, Bong-Kyung (Department of Pathology, Guro Hospital, Korea University Medical College) ;
  • Lee, Young-Suk (Department of Pathology, Guro Hospital, Korea University Medical College) ;
  • Jeong, Hoi-Seon (Department of Pathology, Guro Hospital, Korea University Medical College) ;
  • Lee, Sang-Ho (Department of Pathology, Guro Hospital, Korea University Medical College) ;
  • Kim, Hyun-Chul (Department of Pathology, Guro Hospital, Korea University Medical College) ;
  • Kim, A-Ree (Department of Pathology, Guro Hospital, Korea University Medical College) ;
  • Kim, In-Sun (Department of Pathology, Guro Hospital, Korea University Medical College) ;
  • Kim, Han-Kyeom (Department of Pathology, Guro Hospital, Korea University Medical College)
  • 신봉경 (고려대학교 구로병원 병리과) ;
  • 이영석 (고려대학교 구로병원 병리과) ;
  • 정회선 (고려대학교 구로병원 병리과) ;
  • 이상호 (고려대학교 구로병원 병리과) ;
  • 김현철 (고려대학교 구로병원 병리과) ;
  • 김애리 (고려대학교 구로병원 병리과) ;
  • 김인선 (고려대학교 구로병원 병리과) ;
  • 김한겸 (고려대학교 구로병원 병리과)
  • Published : 2008.09.30
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Abstract

Urothelial carcinoma accounts for 90% of all the cases of bladder cancer. Although many cases can be easily managed by local excision, urothelial carcinoma rather frequently recurs, tends to progress to muscle invasion, and requires regular follow-ups. Urine cytology is a main approach for the follow-up of bladder tumors. It is noninvasive, but it has low sensitivity of around 50% with using the conventional cytospin preparation. Liquid-based cytology (LBC) has been developed as a replacement for the conventional technique. We compared the cytomorphometric parameters of $ThinPrep^{(R)}$ and cytospin preparation urine cytology to see whether there are definite differences between the two methods and which technique allows malignant cells to be more effectively discriminated from benign cells. The nuclear-to-cytoplasmic ratio value, as measured by digital image analysis, was efficient for differentiating malignant and benign urothelial cells, and this was irrespective of the preparation method and the tumor grade. Neither the $ThinPrep^{(R)}$ nor the conventional preparation cytology was definitely superior for distinguishing malignant cells from benign cells by cytomorphometric analysis of the adequately preserved cells. However, the $ThinPrep^{(R)}$ preparation showed significant advantages when considering the better preservation and cellularity with a clear background.

Keywords

References

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