• 제목/요약/키워드: morphometric analysis

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한국에 서식하고 있는 설치류의 계통분류학적 연구: 6. 집쥐속 2종의 형태학적 형질, 염색체 핵형 및 미토콘디리아 DNA의 분석 (Systematic Studies on Korean Rodents: VI. Analysis of Morphometric Characters, Chromosomal Karyotypes and Mitochondrial DNA in Two Species of Genus Rattus)

  • 고홍선
    • Animal Systematics, Evolution and Diversity
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    • 제8권2호
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    • pp.231-242
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    • 1992
  • 한국에 서식하고 잇는 집쥐속 2종 (곰쥐, Rattus rattus Linnaeus; 집쥐, Rattus norvegicus Berkenhaut)의 채집된 표본들의 31개 형태적 형질들을 통계적으로 분석하여 종내 지리적 변이와 종간 차이를 구명하였다. 염색체 G-bands와 C-bands도 비교하였으며, 미토콘드리아 DNA의 제한요소에 의한 절단 단편들의 분석도 하였다. 한국내 여섯지역의 곰쥐들은 형태적 형질에 있어서 서로 비슷하였다: 두동장, 미장, 염색체 핵형과 C-bands에 있어서는 일본산 곰쥐인 Rattus rattus tanezumi와 유사하였다. 한국내 일곱지역의 집쥐들은 형태적 형질에 있어서 서로 비슷하였다: 염색체 핵형은 동부 아시아산 집쥐인 Rattus norvegicus caraco와 유사하였다. 집쥐와 곰쥐는 형태적으로 형질뿐만 아니라, 염색체 핵형과 미토콘드리아 DNA도 차이가 있었다. 한국에 서식하고 있는 곰쥐의 옳바른 학명은 Rattus rattus tanezumi Temminck이고, 집쥐의 학명은 Rattus norvegicus caraco Pallas이며, 집쥐는 곰쥐와 다른 종임이 확인되었다.

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Morphological Studies on the Jack Mackerel Populations

  • Kim, Wan Soo
    • 한국해양학회지
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    • 제4권1호
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    • pp.17-35
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    • 1969
  • This work concerns morphological studies of the Japaness jack mackerel, Trachurus japonicus(Temminck & Schlegel). A total of 48 random samples consisting of 2669 fish is examined, representing three geographic regions, namely, Kamakura, Nagasaki, and Tottori. Preservation affects length and weight of fish considerably, but the variability after 10 days is shown to be negligible. The variability in measuring the morphometric characters due to different measures and orders is found to be significant. They are relatively small, however, compared to the variability in mean length to be expected in samples drawn from the population. Two meristic characters, namely, anal and second dorsal fin rays and three morphometric characters, namely, head length, first and second dorsal insertion distances are chosen for the study. Results of the statistical analysis reveal that differences in the selected meristic characters among samples within and between regions are found to be significant. In general, it is reasonable to state that the jack mackerel collected in Nagasaki region, on the average, have greatest number of anal fin ray and second dorsal fin ray followed by Kamakura, and Tottori regions in that order. It is found that although no significant differences in the slope of regression line are noticed, the mean differences of the selected morphometric measurements in relation to fish length are found to be significant among samples within each region. Differences in the regression coefficients as well as the adjusted sample means are found to be significant between regions. The analysis suggests that samples from Nagasaki region, on the average, have longest head length and greatest distances from snout to the insertion of first dorsal and second dorsal fins followed by Kamakura, and Tottori regions in that order.

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Morphometric and Genetic Variation of Tropilaelaps Mites Infesting Apis dorsata and A. mellifera in Thailand

  • Suppasat, Tipwan;Wongsiri, Siriwat
    • 한국양봉학회지
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    • 제33권4호
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    • pp.227-237
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    • 2018
  • The majority parasitic bee mites of Thailand in genus Tropilaelaps are infesting colonies of native bees (Apis dorsata) and introduced bees (A. mellifera). The investigation aims to study morphological and genetic variation of Tropilaelaps mites infected different hosts. Adult mites were collected from honey bee brood throughout Thailand. Traditional and geometrical morphometrics were measured on photograph by using TPS program. Additional, COI gene variations were examined by PCR-RFLP and nucleotides sequencing. Tree of mites relationships were constructed by NJ and MP assumptions. Morphometric results indicated T. mercedesae were major species infesting on A. dorsata and A. mellifera. Mophological variation represented at anal and epigynial plate, which the shape of the anal plate apex margin has been key character to identify between T. mercedesae (bell to blunt shape) and T. koenigerum (pear shape). However, the discriminant analysis suggested that geometric results were potential to classify Thai Tropilaelaps populations from different hosts better than traditional morphometric. Otherwise, PCR-RFLP clearly detected the site of Dra I and Xba I digestion of Thai Tropilaelaps morphotypes. The COI sequences of T. koenigerum were founded infesting only A. dorsata in Thailand and four sequences that related to the Thai T. mercedesae morphotypes. The NJ and MP tree were clearly classified Thai Tropilaelaps species which were suggested both from morphological and molecular analysis. This information might be basically of taxonomic status, but this should have implication for controlling these mites in Thailand and other countries.

한국산 재래꿀벌의 전자계량형태학적 분류. II. 전 47형질에 대한 각 지역개체군간 판정분석 (Electron-Morphometric Classification of the Native Honeybees from Korea. Part II. Discriminant Analysis for Different Populations Based on the Total Characters)

  • 권용정;허은엽
    • 한국응용곤충학회지
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    • 제32권1호
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    • pp.30-41
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    • 1993
  • 우리나라에 분포하고 있는 재래꿀벌(Apis cerana)의 일벌(worker)을 대상으로 춘계 15지역 및 하계 16지역 개체군을 선택하였으며, 총 47개 정량형질에 대해 계절 및 개체군별로 판별분석(discriminant analysis)을 실시하였다. 그 결과, 각 계절별 및 개체군별 분리도는 모든 비교방법에서 매우 뚜렷하였다. 특히, 전체 47형질 중 앞다리 경절 길이(FTL)가 분리 기여도가 가장 큰 형질로 나타났다.

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각시붕어 Rhodeus uyekii와 떡납줄갱이 R. notatus 간 정교배체, 상반교배체의 계측형질과 세포유전학적 분석 (Morphometric Traits and Cytogenetic Analysis in Induced Cross and Reciprocal Hybrid between Rhodeus uyekii and R. notatus)

  • 김봉석;강언종;장혁;박인석
    • 한국어류학회지
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    • 제24권3호
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    • pp.151-159
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    • 2012
  • 정교배체인 각시붕어, Rhodeus uyekii (♀)${\times}$떡납줄갱이, R. notatus (♂) 잡종, 그리고 상반교배체인 떡납줄갱이 (♀)${\times}$각시붕어(♂) 잡종을 대상으로 혈액도말을 통한 적혈구 크기, flowcytometry를 통한 세포유전학적인 형질 및 truss dimension과 classical dimension에 의한 외부 계측형질에서의 특성을 부모종간의 차이를 비교하였다. 각시붕어와 떡납줄갱이의 정교배체 및 상반교배체의 계측형질은 일반적으로 이들 유도시 사용된 모계와 부계 종의 중간을 보였다. 정교배체는 14개의 계측형질이 부계의 형질과 유사하였으며 7개의 계측형질이 모계의 형질과 유사하였다. 상반교배체의 경우, 13개의 계측형질이 부계의 형질을, 5개의 계측형질이 모계의 형질과 유사하였다. 정교배체와 상반교배체의 체색소 분포는 중간을 보이나 양친 종을 닮게 나타났다(P<0.05). 적혈구 크기와 DNA 함량은 정교배체와 상반교배체 모두 모계의 적혈구 크기 및 DNA 함량을 따르는 경향이 나타났다(P<0.05). 본 연구에서 파악된 각시붕어와 떡납줄갱이의 잡종 계측형질 및 세포유전학적인 형질은 납자루아과의 관상어 개발시 종간 구별에 유용할 것이다.

Morphometric Characterisation of Root-Knot Nematode Populations from Three Regions in Ghana

  • Nyaku, Seloame Tatu;Lutuf, Hanif;Cornelius, Eric
    • The Plant Pathology Journal
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    • 제34권6호
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    • pp.544-554
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    • 2018
  • Tomato (Solanum lycopersicum) production in Ghana is limited by the root-knot nematode (Meloidogyne incognita, and yield losses over 70% have been experienced in farmer fields. Major management strategies of the root-knot nematode (RKN), such as rotation and nematicide application, and crop rotation are either little efficient and harmful to environments, with high control cost, respectively. Therefore, this study aims to examine morphometric variations of RKN populations in Ghana, using principal component analysis (PCA), of which the information can be utilized for the development of tomato cultivars resistant to RKN. Ninety (90) second-stage juveniles (J2) and 16 adult males of M. incognita were morphometrically characterized. Six and five morphometric variables were measured for adult males and second-stage juveniles (J2) respectively. Morphological measurements showed differences among the adult males and second-stage juveniles (J2). A plot of PC1 and PC2 for M. incognita male populations showed clustering into three main groups. Populations from Asuosu and Afrancho (Group I) were more closely related compared to populations from Tuobodom and Vea (Group II). There was however a single nematode from Afrancho (AF4) that fell into Group III. Biplots for male populations indicate, body length, DEGO, greatest body width, and gubernaculum length serving as variables distinguishing Group 1 and Group 2 populations. These same groupings from the PCA were reflected in the dendogram generated using Agglomerative Hierarchical Clustering (AHC). This study provides the first report on morphometric characterisation of M. incognita male and juvenile populations in Ghana showing significant morphological variation.

Morphometric variation, genetic diversity and allelic polymorphism of an underutilised species Thaumatococcus daniellii population in Southwestern Nigeria

  • Animasaun, David Adedayo;Afeez, Azeez;Adedibu, Peter Adeolu;Akande, Feyisayo Priscilla;Oyedeji, Stephen;Olorunmaiye, Kehinde Stephen
    • Journal of Plant Biotechnology
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    • 제47권4호
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    • pp.298-308
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    • 2020
  • Genetic diversity among Thaumatococcus daniellii populations in the southwestern region of Nigeria were assessed using morphometric and molecular markers to determine the population structure and existing genetic relationship for its improvement, conservation and sustainable utilisation. Populations from five locations in each of the six states were used for the study. Morphometric data were collected on folia characters and analysed for variability. Genome DNA was isolated from the plant leaf and amplified by polymerase chain reaction with inter-simple sequence repeat markers (ISSR) to determine the allelic polymorphism, marker effectiveness and genetic relationship of the population. The results showed significant variations in petiole length and leaf dimensions of the populations within and across the states. These morphometric traits are the major parameters that delimit the populations and they correlated significantly at P≤0.05. Analysis of the electrophoregram showed that the ISSR markers are effective for the diversity study. A total of 136 loci were amplified with an average of 7.16 loci per marker, 63.2% of the loci were polymorphic. The Principal Coordinate Analysis revealed that seven factors accounted for 81.6% of the variation and the dendrogram separated the populations into two major groups at a genetic distance of 10 (about 90% similarity) with sub-groups and clusters. Most populations within the state had a high degree of similarity, nonetheless, strong genetic relationship exists among populations from different states. The close relationship between populations across the states suggests a common progenitor, which are likely separated by ecological or geographical isolation mechanisms.

Morphometric analysis of the inter-mastoid triangle for sex determination: Application of statistical shape analysis

  • Sobhani, Farshad;Salemi, Fatemeh;Miresmaeili, Amirfarhang;Farhadian, Maryam
    • Imaging Science in Dentistry
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    • 제51권2호
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    • pp.167-174
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    • 2021
  • Purpose: Sex determination can be done by morphological analysis of different parts of the body. The mastoid region, with its anatomical location at the skull base, is ideal for sex identification. Statistical shape analysis provides a simultaneous comparison of geometric information on different shapes in terms of size and shape features. This study aimed to investigate the geometric morphometry of the inter-mastoid triangle as a tool for sex determination in the Iranian population. Materials and Methods: The coordinates of 5 landmarks on the mastoid process on the 80 cone-beam computed tomographic images(from individuals aged 17-70 years, 52.5% female) were registered and digitalized. The Cartesian x-y coordinates were acquired for all landmarks, and the shape information was extracted from the principal component scores of generalized Procrustes fit. The t-test was used to compare centroid size. Cross-validated discriminant analysis was used for sex determination. The significance level for all tests was set at 0.05. Results: There was a significant difference in the mastoid size and shape between males and females(P<0.05). The first 2 components of the Procrustes shape coordinates explained 91.3% of the shape variation between the sexes. The accuracy of the discriminant model for sex determination was 88.8%. Conclusion: The application of morphometric geometric techniques will significantly impact forensic studies by providing a comprehensive analysis of differences in biological forms. The results demonstrated that statistical shape analysis can be used as a powerful tool for sex determination based on a morphometric analysis of the inter-mastoid triangle.

가시황백매미충아속의 계량형태학적 분류(매미목: 매미충과) (Morphometric Classification of the Subgenus Parapagaronia from Korea (Homoptera: Cicadellidae))

  • 허은엽;권용정
    • 한국응용곤충학회지
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    • 제35권1호
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    • pp.7-14
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    • 1996
  • 가시황백매미충아속(Parapagaronia)의 였으나, 암컷에서는 96.25%로 더 높게나타났다. 계량형태학적 수리분류의 일환으로서 4종을 대상으로 정량적 형태형질을 이용한 다변량분석을 시도한 결과, 각 종간 평균분리솔의 수컷에서는 87.5%였으나, 암컷에서는 96.25%로 더 높게 나타났다.

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한국산 설치류의 계통분류학적 연구 1.등줄쥐, Apodemus agrarius coreae Thomas 의 형태적 형질의 지리적 변이 (: I. Geographic Variation of Morphometric Characters in Striped Field Mice, Apodemus agrarius coreae Thomas)

  • Koh, Hung-Sun
    • 한국동물학회지
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    • 제28권1호
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    • pp.9-20
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    • 1985
  • 태백산, 월악산, 팔공산 및 청주지역에서 채집한 등줄쥐, Apodemus agrarius coreae를 사용하여 형태적 형질의 단변량분석 (univariate analysis)과 다변량분석 (multivariate analysis)을 행하였다. 채집된 표본들은 서로 유사하여 동일아종임이 재입증되었다. 표본이 채집되었던 지역의 고도와 연관된 clinal variation이 discriminant analysis의 제일축(first axis)과 꼬리의 길이(length of tail vertebrae)에서 나타났다.

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