• Title/Summary/Keyword: morphology formation

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The Sensor Kinase GacS Negatively Regulates Flagellar Formation and Motility in a Biocontrol Bacterium, Pseudomonas chlororaphis O6

  • Kim, Ji Soo;Kim, Yong Hwan;Anderson, Anne J.;Kim, Young Cheol
    • The Plant Pathology Journal
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    • v.30 no.2
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    • pp.215-219
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    • 2014
  • The GacS/GacA two component system regulates various traits related to the biocontrol potential of plant-associated pseudomonads. The role of the sensor kinase, GacS, differs between strains in regulation of motility. In this study, we determined how a gacS mutation changed cell morphology and motility in Pseudomonas chlororaphis O6. The gacS mutant cells were elongated in stationary-phase compared to the wild type and the complemented gacS mutant, but cells did not differ in length in logarithmic phase. The gacS mutant had a two-fold increase in the number of flagella compared with the wild type strain; flagella number was restored to that of the wild type in the complemented gacS mutant. The more highly flagellated gacS mutant cells had greater swimming motilities than that of the wild type strain. Enhanced flagella formation in the gacS mutant correlated with increased expression of three genes, fleQ, fliQ and flhF, involved in flagellar formation. Expression of these genes in the complemented gacS mutant was similar to that of the wild type. These findings show that this root-colonizing pseudomonad adjusts flagella formation and cell morphology in stationary-phase using GacS as a major regulator.

Mimicking the pattern formation of fruits and leaves using gel materials

  • Chen, Li;Zhang, Yang;Swaddiwudhipong, Somsak;Liu, Zishun
    • Structural Engineering and Mechanics
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    • v.50 no.5
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    • pp.575-588
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    • 2014
  • Gel materials have recently gained more attention due to its unique capability of large and reversible volumetric changes. This study explores the possibility of mimicking the pattern formation of certain natural fruits during their growing process and leaves during drying processes through the swelling and de-swelling of gel materials. This will hopefully provide certain technical explanations on the morphology of fruits and plants. We adopt the inhomogeneous field gel theory to predict the deformation configurations of gel structures to describe the morphology of natural fruits and plants. The growing processes of apple and capsicum are simulated by imposing appropriate boundary conditions and field loading via varying the chemical potential from their immature to mature stages. The drying processes of three types of leaves with different vein structures are also investigated. The simulations lead to promising results and demonstrate that pattern formation of fruits and plants may be described from mechanical perspective by the behavior of gel materials based on the inhomogeneous field theory.

Formation Mechanism of Sr-Ferrite by Molten Salt Method (용융염법에 의한 Sr-ferrite의 생성기구)

  • 박준홍;신효순;이병교
    • Journal of the Korean Ceramic Society
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    • v.31 no.10
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    • pp.1181-1187
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    • 1994
  • Sr-ferrite powders were synthesized by molten salt method. The process of powder formation was investigated by controlling the size and shape (sphere and acicular) of starting materials. The morphology of resulting ferrite was plate-like regardless of the shape of starting materials, Fe2O3 powders. As a result, the formation of Sr-ferrite in the molten salt was proceeded by solution-precipitation.

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Examining the star formation properties of Virgo galaxies undergoing ram pressure stripping

  • Mun, Jae Yeon;Hwang, Ho Seong;Chung, Aeree;Yoon, Hyein;Lee, Myung Gyoon
    • The Bulletin of The Korean Astronomical Society
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    • v.44 no.1
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    • pp.75.3-75.3
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    • 2019
  • Understanding how ram pressure stripping (RPS) affects the star formation activity of cluster galaxies is one of the important issues in astrophysics. To examine whether we can identify any discernible trend in the star formation activity of galaxies undergoing ram pressure stripping, we study the star formation properties of galaxies in the Virgo cluster for which high-resolution HI images are available. We first classify galaxies in the Extended Virgo Cluster Catalog into different stages of RPS based on their HI morphology, HI deficiency, and location in phase space. We then examine various star formation activity indicators of these galaxies, which include starburstiness, g - r color, and WISE [3.4]-[12] color. No noticeable enhancement in star formation was identified for galaxies undergoing early or active stripping. Our results suggest that star formation activity at best seems to be enhanced locally in such galaxies, making it challenging to detect with integrated photometry. With the combination of HI deficiencies and locations in phase space, we were instead able to capture the overall quenching of star formation activity with increasing degree of ram pressure stripping, which agree with previous studies.

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Influence of Surface Morphology and Substrate on Thermal Stability and Desorption Behavior of Octanethiol Self-Assembled Monolayers

  • Ito, Eisuke;Gang, Hun-Gu;Ito, Hiromi;Hara, Masahiko;No, Jae-Geun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.08a
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    • pp.219-219
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    • 2012
  • The formation and thermal desorption behaviors of octanethiol (OT) SAMs on single crystalline Au (111) and polycrystalline Au, Ag, and Cu substrates were examined by X-ray photoelectron microscopy (XPS), thermal desorption spectroscopy (TDS), and contact angle (CA) measurements. XPS and CA measurements revealed that the adsorption of octanethiol (OT) molecules on these metals led to the formation of chemisorbed self-assembled monolayers (SAMs). Three main desorption fragments for dioctyl disulfide (C8SSC8+, dimer), octanethiolate (C8S+), and octanethiol (C8SH+) were monitored using TDS to understand the effects of surface morphology and the nature of metal substrates on the thermal desorption behavior of alkanethiols. TDS measurements showed that a sharp dimer peak with a very strong intensity on single crystalline Au (111) surface was dominantly observed at 370 K, whereas a broad peak on the polycrystalline Au surface was observed at 405 K. On the other hand, desorption behaviors of octanethiolates and octanethiols were quite similar. We concluded that substrate morphology strongly affects the dimerization process of alkanethiolates on Au surfaces. We also found that desorption intensity of the dimer is in the order of Au>>Ag>Cu, suggesting that the dimerization process occurs efficiently when the sulfur-metal bond has a more covalent character (Au) rather than an ionic character (Ag and Cu).

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Effect of Titanium Coating on Cell Adhesion and Extracellular Matrix Formation in Human Osteoblast-like MG-63 Cells

  • Lee, Jae-Bum;Seo, Sang-Hui;Kim, Yu-Ri;Shin, Sang-Wan;Kim, Meyoung-Kon;Ryu, Jae-Jun
    • Molecular & Cellular Toxicology
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    • v.4 no.3
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    • pp.192-198
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    • 2008
  • A variety of titanium (Ti) and its alloys are used in the clinical procedures of bone regeneration for periodontal and dental implant therapies. This study was performed to determine the effect of different surface dental implant materials on biologic responses of a MG-63 human osteoblast-like cell line. MG-63 cells were cultured on Ti coated with hydroxyapatite (HA), calcium metaphosphate (CMP), anodized (A), which compared with non-coated Ti (control). The appearances of surface of dental implant materials and the morphology of these cells were assessed by scanning electron microscopy (SEM). The gene expression profiles of MG-63 cells cultured on Ti were examined by human cDNA microarray (1,152 elements). The expression of several genes was up- and down-regulated by different surfaces of dental implant materials. Interesting, the genes correlated with cellular adhesion and extra cellular matrix (ECM) formation were enhanced, in accordance surface morphology of the dental implant materials used.

Effects of Penicillin G on Morphology and Certain Physiological Parameters of Lactobacillus acidophilus ATCC 4356

  • Khaleghi, M.;Kermanshahi, R. Kasra;Zarkesh-Esfahani, S.H.
    • Journal of Microbiology and Biotechnology
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    • v.21 no.8
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    • pp.822-829
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    • 2011
  • Evidence shows that probiotic bacteria can undergo substantial structural and morphological changes in response to environmental stresses, including antibiotics. Therefore, this study investigated the effects of penicillin G (0.015, 0.03, and 0.06 mg/l) on the morphology and adhesion of Lactobacillus acidophilus ATCC 4356, including the colony morphotype, biofilm production, hydrophobicity, $H_2_O2$ formation, S-layer structure, and slpA gene expression. Whereas only smooth colonies grew in the presence of penicillin, rough and smooth colony types were observed in the control group. L. acidophilus ATCC 4356 was found to be hydrophobic under normal conditions, yet its hydrophobicity decreased in the presence of the antibiotic. No biofilm was produced by the bacterium, despite testing a variety of different culture conditions; however, treatment with penicillin G (0.015-0.06 mg/l) significantly decreased its production of $H_2_O_2$ formation and altered the S-layer protein structure and slpA gene expression. The S-protein expression decreased with 0.015 mg/l penicillin G, yet increased with 0.03 and 0.06 mg/l penicillin G. In addition, the slpA gene expression decreased in the presence of 0.015 mg/l of the antibiotic. In conclusion, penicillin G was able to alter the S-layer protein production, slpA gene expression, and certain physicochemical properties of Lactobacillus acidophilus ATCC 4356.

Effects of Charge-discharge Rate on Morphology and Resistance of Surface Film on a Graphite Negative Electrode in an Ethylene Carbonate-based Solution (탄산 에틸렌계 용액 중에서 생성되는 흑연 음극 표면피막의 형상 및 저항에 미치는 충방전 속도의 영향)

  • Jeong, Soonki;Kim, Pogyom
    • Journal of Hydrogen and New Energy
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    • v.24 no.2
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    • pp.179-185
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    • 2013
  • The behavior of surface film formation was greatly dependent on the speed of potential cycling. In $LiClO_4$ / EC + DEC, cyclic voltammetry results showed that the peaks originated from surface film formation on graphite electrode at the high charge-discharge rate was shifted to the lower potentials as the charge-discharge rate decrease. This indicates that surface films with different morphology and thickness were formed by different charge-discharge rate. Transmission electron microscopy (TEM) results indicated that the properties such as thickness and morphology of the surface film were greatly affected by the charge-discharge rate. Electrochemical impedance spectroscopy (EIS) showed that the resistance of surface film was affected by the speed of potential cycling. In addition, the charge transfer resistance was also dependent on the charge-discharge rate indicating that the charge transfer reaction was affected by the nature of surface film. TEM and EIS results suggested that the chemical property as well as the physical property of the surface film was affected by the charge-discharge rate.

Morphology Development in a Range of Nanometer to Micrometer in Sulfonated Poly(ethylene terephthalate) Ionomer

  • Lee, Chang-Hyung;Inoue, Takashi;Nah, Jae-Woon
    • Bulletin of the Korean Chemical Society
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    • v.23 no.4
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    • pp.580-586
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    • 2002
  • We investigated the effect of ionic component on crystalline morphology development during isothermal annealing in a sodium neutralized sulfonated poly(ethylene terephthalate) ionomer (Ion-PET) by time-resolved small-angle x-ray scattering (TR-SAX S) using synchrotron radiation. At early stage in Ion-PET, SAXS intensity at a low annealing temperature (Ta = 120 $^{\circ}C)$ decreased monotonously with scattering angle for a while. Then SAXS profile showed a peak and the peak position progressively moved to wider angles with isothermal annealing time. Finally, the peak intensity decreased, shifting the peak angle to wider angle. It is revealed that ionic aggregates (multiplets structure) of several nm, calculated by Debye-Bueche plot, are formed at early stage. They seem to accelerate the crystallization rate and make fine crystallites without spherulite formation (supported by optical microscopy observation). From decrease of peak intensity in SAXS,it is suggested that new lamellae are inserted between the preformed lamellae so that the concentration of ionic multiplets in amorphous region decreases to lower the electron density difference between lamellar crystal and amorphous region. In addition, analysis on the annealing at a high temperature (Ta = 210 $^{\circ}C)$ by optical microscopy, light scattering and transmission electron microscopy shows a formation of spherulite, no ionic aggregates, the retarded crystallization rate and a high level of lamellar orientation.

IMPACT OF NEIGHBORS IN SDSS GALAXY PAIRS

  • MOON, JUN-SUNG;YOON, SUK-JIN
    • Publications of The Korean Astronomical Society
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    • v.30 no.2
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    • pp.469-471
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    • 2015
  • How galaxies are affected by their neighboring galaxies during galaxy-galaxy interactions is a long-standing question. We investigate the role of neighbors in galaxy pairs based on the SDSS data release 7 and the KIAS value-added galaxy catalog. Three groups of galaxies are identified: (a) galaxies with an early-type neighbor, (b) with a late-type neighbor, and (c) isolated ones with no neighbor. We compare their UV + optical colors and $H{\alpha}$ emission as indicators of the recent star-formation rate (SFR). Given that galaxies show systematic differences in SFR as functions of morphology, luminosity, and large-scale environments, we construct a control sample in which the galaxies have the same conditions (in terms of morphology, luminosity, and large-scale environment) except for the neighbor's properties (i.e., morphology, mass, and distance). The results are as follows. (1) Galaxies with a late-type companion demonstrate more enhanced SFR than those with an early-type companion. (2) Galaxies with an early-type neighbor show NUV- and u-band derived SFRs that are even lower than that of isolated galaxies, while they have similar or slightly higher $H{\alpha}$-based SFR compared to isolated ones.