• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.847-859
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• 2022

The development and commercialization of industrial genetically modified (GM) organisms is actively progressing worldwide, highlighting an increased need for improved safety management protocols. We sought to establish an environmental monitoring method, using real-time polymerase chain reaction (PCR) and propidium monoazide (PMA) treatment to develop a quantitative detection protocol for living GM microorganisms. We developed a duplex TaqMan quantitative PCR (qPCR) assay to simultaneously detect the selectable antibiotic gene, ampicillin (AmpR), and the single-copy Escherichia coli taxon-specific gene, D-1-deoxyxylulose 5-phosphate synthase (dxs), using a direct cell suspension culture. We identified viable engineered E. coli cells by performing qPCR on PMA-treated cells. The theoretical cell density (true copy numbers) calculated from mean quantification cycle (Cq) values of PMA-qPCR showed a bias of 7.71% from the colony-forming unit (CFU), which was within ±25% of the acceptance criteria of the European Network of GMO Laboratories (ENGL). PMA-qPCR to detect AmpR and dxs was highly sensitive and was able to detect target genes from a 10,000-fold (10^-4) diluted cell suspension, with a limit of detection at 95% confidence (LOD_95%) of 134 viable E. coli cells. Compared to DNA-based qPCR methods, the cell suspension direct PMA-qPCR analysis provides reliable results and is a quick and accurate method to monitor living GM E. coli cells that can potentially be released into the environment.

• Journal of Internet of Things and Convergence
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• v.3 no.2
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• pp.21-32
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• 2017

This study is a descriptive investigation for understanding factors influencing the length of stay in the postanesthesia care unit of elderly patients. Retrospective investigation was conducted on 300 patients aged 65 or older among those who had received an operation under general anesthesia and treated in postanesthesia care unit of hospital C located in Gyeonggi-do, from January 1 to December 31, 2014. The patients' average postanesthesia care unit stay was found to be 48.4 minutes and the 30-59 minute section accounted for the highest part with 58.0%. The postanesthesia care unit stay time according to intra-operative factors showed significant differences depending upon muscle relaxants, transfusion, ABGA, body temperature and total hours under anesthesia. Concerning the post anesthesia care unit stay length according to post-operative factors, significant differences were observed depending upon complications, PCA device, and circulatory drug use. In order to find out factors influencing the post anesthesia care unit stay length, the multiple regression analysis was conducted. As a result, the circulatory drug use and intra-operative lower body temperature were found to have an effect on the post anesthesia care unit stay length with the total explanatory power of 13%. Based on these findings above, it is deemed helpful to carefully monitor factors related to the post anesthesia care unit stay length and provide swift response accordingly for shorter post anesthesia care unit stay time of elderly general anesthesia patients.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.4
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• pp.73-81
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• 2020

This study compared nursing service satisfaction and revisit intention of comprehensive nursing care unit and general care unit patients and confirmed the impact of nursing service satisfaction on revisit intention. We used data from 201 survey questionnaires collected from four general hospitals located in B-metropolitan area. Data were collected from Jun 1 to 30, 2019. The data were analyzed using x²-test, t-test, ANOVA, Scheffé test, Pearson's Correlation Coefficient, and Stepwise multiple regression. The results show that nursing service satisfaction was significantly higher in patients admitted to the comprehensive nursing care unit than in the general care unit. There was a significant correlation in increased nursing service satisfaction being associated with increased revisit intention. Among subcategories of nursing service satisfaction, Empathy and Tangibles were cited by 56% of the comprehensive nursing care unit and 40% of the general care unit. As a result of this study, it is necessary to prepare a nursing system that can continuously monitor nursing service satisfaction in order to increase hospital revisit intention and expand the implementation of a comprehensive nursing care unit system.

• Parasites, Hosts and Diseases
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• v.58 no.6
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• pp.627-634
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• 2020

Belitung district in Bangka-Belitung Province, Indonesia with a population of 0.27 million is endemic for Brugia malayi and 5 rounds of mass drug administration (MDA) were completed by 2010. Based on the results of 3 transmission assessment surveys (TAS), the district is declared as achieving elimination of lymphatic filariasis (LF) in 2017. The findings of an independent survey conducted by the National Institute of Health Research and Development (NIHRD) in the same year showed microfilaria (Mf) prevalence of 1.3% in this district. In 2019, NIHRD conducted microfilaria survey in 2 villages in Belitung district. Screening of 311 and 360 individuals in Lasar and Suak Gual villages showed Mf prevalence of 5.1% and 2.2% with mean Mf density of 120 and 354 mf/ml in the respective villages. Mf prevalence was significantly higher among farmers and fishermen compared to others and the gender specific difference was not significant. The results of a questionnaire based interview showed that 62.4% of the respondents reported to have participated in MDA in Lasar while it was 57.7% in Suak Gual village. About 42% of the Mf positive cases did not participate in MDA. Environmental surveys identified many swampy areas supporting the breeding of Mansonia vector species. Persistence of infection is evident and in the event of successful TAS3 it is necessary to monitor the situation and plan for focal MDA. Appropriate surveillance strategies including xenomonitoring in post-MDA situations need to be developed to prevent resurgence of infection. Possible role of animal reservoirs is discussed.

• JSTS:Journal of Semiconductor Technology and Science
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• v.2 no.2
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• pp.132-140
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• 2002

This paper presents an ARM-based SOC bus transaction verification IP and the usage experiences in SOC designs. The verification IP is an AMBA AHB protocol checker, which captures legal AHB transactions in FSM-style signal sequence checking routines. This checker can be considered as a reusable verification IP since it does not change unless the bus protocol changes. Our AHB protocol checker is designed to be scalable to any number of AHB masters and reusable for various AMBA-based SOC designs. The keys to the scalability and the reusability are Object-Oriented Programming (OOP), virtual port, and bind operation. This paper describes how OOP, virtual port, and bind features are used to implement AHB protocol checker. Using the AHB protocol checker, an AHB simulation monitor is constructed. The monitor checks the legal bus arbitration and detects the first cycle of an AHB transaction. Then it calls AHB protocol checker to check the expected AHB signal sequences. We integrate the AHB bus monitor into Verilog simulation environment to replace time-consuming visual waveform inspection, and it allows us to find design bugs quickly. This paper also discusses AMBA AHB bus transaction coverage metrics and AHB transaction coverage analysis. Test programs for five AHB masters of an SOC, four channel DMAs and a host interface unit are executed and transaction coverage for DMA verification is collected during simulation. These coverage results can be used to determine the weak point of test programs in terms of the number of bus transactions occurred and guide to improve the quality of the test programs. Also, the coverage results can be used to obtain bus utilization statistics since the bus cycles occupied by each AHB master can be obtained.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.595-602
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• 2005

A one-step real-time quantitative RT-PCR assay in combination with automated RNA extraction was evaluated for routine testing of HCV RNA in the laboratory. Specific primers and probes were developed to detect 302 bp on 5'-UTR of HCV RNA. The assay was able to quantitate a dynamic linear range of $10^7-10^1$ HCV RNA copies/reaction ($R^2=0.997$). The synthetic HCV RNA standard of $1.84{\pm}0.1\;(mean{\pm}SD)$ copies developed in this study corresponded to 1 international unit (IU) of WHO International Standard for HCV RNA (96/790 I). The detection limit of the assay was 3 RNA copies/reaction (81 IU/ml) in plasma samples. The assay was comparable to the Amplicor HCV Monitor (Monitor) assay with correlation coefficient r=0.985, but was more sensitive than the Monitor assay. The assay could be completed within 3 h from RNA extraction to detection and data analysis for up to 32 samples. It allowed rapid RNA extraction, detection, and quantitation of HCV RNA in plasma samples. The method provided sufficient sensitivity and reproducibility and proved to be fast and labor-saving, so that it was suitable for high throughput HCV RNA test.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2003.09a
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• pp.82-82
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• 2003

Introduction: With the development of dose calculation algorithms for electron beams, 3D RTP systerns are available for electron beam dose distribution commercially. However, no studies evaluated the accuracy of dose calculation with ADAC Pinnacle system for electron beams. So, the accuracy of the ADAC system is investigated by comparing electron dose distributions from ADAC system against the BEAMnrc/DOSXYZnrc. Methods: A total of 33 breast cancer patients treated with 6, 9, and 12MeV electrons in our institution was selected for this study. The first part of this study is to compare the dose distributions of measurement, TPS and the BEAMnrc/DOSXYZnrc code in flat water phantom at gantry zero position and for a 10 ${\times}$ 10 $\textrm{cm}^2$ field. The second part is to evaluate the monitor unit obtained from measurement and TPS. Adding actual breast patient's irregular blocks to the first part, monitor units to deliver 100 cGy to the dose maximum (dmax) were calculated from measurement and 3D RTP system. In addition, the dose distributions using blocks were compared between TPS and the BEAMnrc/DOSXYZnrc code. Finally, the effects of tissue inhomogeneities were studied by comparing dose distributions from Pinnacle and Monte Carlo method on CT data sets. Results: The dose distributions calculated using water phantom by the TPS and the BEAMnrc/ DOSXYZnrc code agreed well with measured data within 2% of the maximum dose. The maximum differences of monitor unit between measured and Pinnacle TPS in flat water phantom at gantry zero position were 4% for 6 MeV and 2% for 9 and 12 MeV electrons. In real-patient cases, comparison of depth doses and lateral dose profiles calculated by the Pinnacle TPS, with BEAMnrc/DOSXYZnrc code has generally shown good agreement with relative difference less than +/-3%. Discussion: For comparisons of real-patient cases, the maximum differences between the TPS and BEAMnrc/DOSXYZnrc on CT data were 10%. These discrepancies were due in part to the inaccurate dose calculation of the TPS, so that it needs to be improved properly. Conclusions: On the basis of the results presented in this study, we can conclude that the ADAC Pinnacle system for electron beams is capable of giving results absolutely comparable to those of a Monte Carlo calculation.

• Journal of Radiation Protection and Research
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• v.41 no.2
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• pp.87-91
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• 2016

Background: A hand-held detector for an emergency response was developed for nuclide identification and to estimate the information of the ambient dose rate in the scene of an accident as well as the radioactivity of the contaminants. Materials and Methods: To achieve this, the most suitable sensor was first selected as a cadmium zinc telluride (CZT) semiconductor and the signal processing unit from a sensor and the signal discrimination and storage unit were successfully manufactured on a printed circuit board. Results and Discussion: The performance of the developed signal processing unit was then evaluated to have an energy resolution of about 14 keV at 662 keV. The system control unit was also designed to operate the CZT detector, monitor the detector, battery, and interface status, and check and transmit the measured results of the ambient dose rate and radioactivity. In addition, a collimator, which can control the inner radius, and the airborne dust sampler, which consists of an air filter and charcoal filter, were developed and mounted to the developed CZT detector for the quick and efficient response of a nuclear accident. Conclusion: The hand-held CZT detector was developed to make the in-situ gamma-ray spectrometry and its performance was checked to have a good energy resolution. In addition, the collimator and the airborne dust sampler were developed and mounted to the developed CZT detector for a quick and efficient response to a nuclear accident.

• Journal of the Korean Society of Industry Convergence
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• v.19 no.2
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• pp.62-68
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• 2016

Laser distance measuring systems are used in many fields with high precision. However, when measuring the reflector such as the mirror and the black color, a laser distance measuring system does not guarantee the measurement accuracy. In order to measure the shape of the cargo, this study proposes the radar distance measurement system. The radar distance measuring system is composed of a distance measuring unit with a 24 GHz antenna module, a signal processing and control board and the 1-axis tilting unit. And, this study developed a monitoring program to monitor the measured data. In order to evaluate performance of the developed system, the distance measurement tests are carried out. The distance error was about 6-15 cm. However, considering the size of the cargo, the precision is not a problem. And, cargo shape was measured by using the distance information measured by the 1-axis tilting unit. It could get a 2 dimension shape profile for the cargo stacked in a yard.

• Journal of Drive and Control
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• v.10 no.2
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• pp.7-12
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• 2013

In this study, a wire/wireless communication system transmitting the operation data of engine from the ER (Engine Room) to the engine controller of ECR(Engine Control Room) has been developed through the communication of ISM(Industrial Science Medical) Band for the test operation environment improvement of medium speed engine. This wire/wireless communication system is composed of the RTU (Remote Terminal Unit) gathering and transmitting engine data as well as the MCU (Master Control Unit) receiving engine status information from the RTU to be sent to the engine controller (PLC). Through this study, a trial product of RTU and MCU has been manufactured. A test bench that has made temperature, pressure and pick-up sensor into a module for the local test of prototype was produced a test bench. In addition, at the same time save the data to a Web server and the smart phone real-time monitoring system has been developed using Wi-Fi communications. The ultimate objective of this study is to develop a wireless smart phone monitoring system of engine for the operator of engine to be able to monitor and control engine status even from the outside of engine room and control room based on this study.