• Journal of the Korean Chemical Society
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• v.47 no.6
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• pp.601-607
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• 2003

We studied on preparation of nanoparticles modified surface using biodegradable polymer, poly(DL-lactide-co-glycolide) (PLGA). Two kinds of PLGA nanoparticles were prepared by a spontaneous emulsification solvent diffusion (SESD) method using cetyltrimethylammonium chloride (CTAC) and tetradecyltrimethylammonium bromide (TTAB) as a cationic surfactant and polyethylene glycol-block-polypropylene glycol copolymer (Lutrol F68) as a nonionic surfactant. Model protein was coated on the surface of nanoparticles by the ionic complexation. The model protein was that influenza vaccine ($H_3N_2,\;H_1N_1$, B strain) labeled with NHS-fluorescein. The sizes of cationic nanoparticles were 140-160 nm and the surface charges were 50-60 mV. The sizes of nonionic nanoprticles were 80-90 nm and the surface charge was -10 mV. After coating vaccine on the surface of nanoparticles, the sizes of cationic nanoparticles were increased to 380-400 nm and the size of nonionic nanoparticles was not increased. The amount of coated vaccine on the cationic nanoparticles was 22.73 ${\mu}g$/mg.

• Membrane Journal
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• v.6 no.4
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• pp.219-226
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• 1996

Rotary disc ultrafiltration module(RDM) was developed for the separation of oil emulsions. This module was devised to reduce the gel polarization phenomenon by alecoupling the operation pressure and the surface velocity of solution in ultrafiltration(UF) processes. Two-shaft engaged disc type RDM having 20 rotary disc membcanes(UOP, USA) was operated under 85kPa vacuum at $25^{\circ}$C. The pressure drop due to slip flow in the two-shaft RDM was found to be proportional to $(2.5{\omega}r)^{2}$. The pure water flux of two-shaft RDM decreased by 9.95% at the angular velocity of 41.89rad/s compared to the decrease of 3.01% for one-shaft RDM at the same velocity. When the angular velocity was changed from 31.42rad/s to 2.62rad/s, the flux decline for 1% cutting oil in two-shaft RDM was 30.16% that is similar to that of one-shaft RDM. Disc gap of 3mm and 7mm did not show any significant differences in the flux for 1% cutting oil solution. A modified model for two-shaft RDM showed good agreement with the experimental results for the cutting oil solution.

• Magazine of the Korean Society of Agricultural Engineers
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• v.30 no.4
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• pp.134-153
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• 1988

This paper was carried out to investigate the existence of a unique stress- strain behavior by obtaining some factors influencing the time dependent stress- strain behavior of clay. The results obtained from this study were summarized as follows ; 1. The relationship between stress ratro and strain in normally consolidated clay was in- dependent on pre-shear consolidation pressure. Therefore, shear strain could be expressed as a function with stress ratio. 2. The constitutive equation of shear strain on Modified Carn Clay Model coincided better with the observed value than Cam Clay Model. 3. The relationships between deviator stress and shear strain, between pore water pressure and shear strain were unified by the mean equivalent pressure. 4. The shear strain contour in norrnally consolidated clay was increased linearly through origin, but that in overconsolidated clay was not in accordance with the result of the former. 5. Because the effective stress path of normally consolidated clay was unified by the mean equivalent pressure, state boundary surface in (e,p,q) space was transformed into two dimensional surface. But it was considered to be suitable that the unified stress- strain in overconsolidated clay be expressed by a function with overconsolidation ratio. 6. The deviator for constant strain was increased linearly with increment of strain rate ($\varepsilon$) on semi-log scale, but pore water pressure was decreased. 7. The behavior of stress relaxation was transformed from linear to curvilinear with inc - rement of strain rate before stress relaxation test, and pore water pressure was increased in total range. 8. The strain of creep was increased linearly with increment of time on semi-log scale. The greater the strain rate before creep test became, the greater the increment of strain of creep became. And the pore water pressure during creep test was increased generally with increment of time on semi-log scale.

• Smart Structures and Systems
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• v.33 no.6
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• pp.399-414
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• 2024

This study discusses the long-term deformation monitoring and shape sensing of bridge girder surfaces with an automated extraction scheme for point clouds in the Region Of Interest (ROI), invariant to the position of a Light Detection And Ranging system (LiDAR). Advanced smart construction necessitates continuous monitoring of the deformation and shape of bridge girders during the construction phase. An automated scheme is proposed for reconstructing geometric model of ROI in the presence of noisy non-stationary background. The proposed scheme involves (i) denoising irrelevant background point clouds using dimensions from the design model, (ii) extracting the outer boundaries of the bridge girder by transforming and processing the point cloud data in a two-dimensional image space, (iii) extracting topology of pre-defined targets using the modified Otsu method, (iv) registering the point clouds to a common reference frame or design coordinate using extracted predefined targets placed outside ROI, and (v) defining the bounding box in the point clouds using corresponding dimensional information of the bridge girder and abutments from the design model. The surface-fitted reconstructed geometric model in the ROI is superposed consistently over a long period to monitor bridge shape and derive deflection during the construction phase, which is highly correlated. The proposed scheme of combining 2D-3D with the design model overcomes the sensitivity of 3D point cloud registration to initial match, which often leads to a local extremum.

• Journal of Korea Water Resources Association
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• v.48 no.9
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• pp.729-741
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• 2015

This study examined the applicability of MBLRP (Modified Bartlett-Lewis Rectangular Pulse) rainfall generation model for an urban flood simulation which is a type of Poisson cluster rainfall generation model. This study constructed XP-SWMM model for Namgajwa area of Hongjecheon basin, which is a two-dimensional pipe network-surface flood simulation program and computed a flood discharge and a flooded area with input data of synthetic rainfall time series of 200 years that were generated by the MBLRP model. This study compared the data of flood with synthetic rainfall and flood with corresponding values which were based on design rainfall. The results showed that the flooded area computed with MBLRP model was somewhat smaller than the corresponding values on the basis of the design. A degree of underestimation was from 8% (5 year) to 34% (200 year) and the degree of underestimation increased as a return period increased. This study is meaningful in that it proposes methodology that enables quantifiability of uncertain variables which are related to a flooding through Monte Carlo analysis of urban flooding simulation and applicability and limitations thereof.

• Journal of Biomedical Engineering Research
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• v.14 no.4
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• pp.307-314
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• 1993

Plasma protein adsorption is the first event in the blood-material interaction and influenc- es subsequent platelet adhesion towards thlㅈombus formation. Thiㅈomboembolic events are strongly influenced by surface characteristics of materials and fluid dynamics inside the blood pump. In vitro flow visualizaion and an amimal experiment with the moving actuator type TAH were Performed in order to investigate fluid dynamic effects on the protein adsorption. The diffel'encl level, j of shear rate inside the ventricle Lvere determined by consid- ering the direction of the major opening of four healt valves in the implanted TAH and the visualized flow patterns as well. Each ventricle of the explanted TAH was sectionalized into 12 segments according to the shear rate level. The adsorbed protein on each segment was quantified using the ELISA method after soaking in 2% (wye)SDS/PBS for two days. Adsorbed protein layer thicknesses Itvere measured by the Immunogotd method under TEM. The SEM observation show that right ventricle (RV) , immobilized with albumin, displayed different degrees of platelet adhesion on each segment, whereas the left ventricle (LV), grafted by PEO-sulronate, indicated nearly , iame platelet adhesion behavior, regardless of shear rates. The surface concentrations of adsorbed proteins in the low shear rate region are hlghel'than those in the high region, which was confirmed statistically. A modified adsorption model of plasma protein onto polyurethane surface was suggested by considering the effect of the fluid dynamic characteristics.

• Journal of the Korean Geotechnical Society
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• v.36 no.12
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• pp.117-124
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• 2020

The present research focuses on a methodology to describe shearing response of clay with respect to temperature. An increase of temperature shifts the normal consolidation line to move down in the plane of void ratio and mean effective stress. The critical state line, however, does not move as much as the normal consolidation line in accordance with temperature increase. As temperature increase, therefore, the difference between the critical state mean effective stress and the pre-consolidation pressure reduces. To reflect this easily, the present study applies a bounding surface consisting of two parts divided by the critical state mean effective stress. This study calibrated a bounding surface for the soft Bangkok clay and performed elemental simulation for undrained triaxial compression tests. The elemental simulation showed that the model can describe the mechanical response upon temperature of clay without complex hardening and evolution rules compared to the experimental data.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.103-103
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• 2003

The purpose of this study is to evaluate an efficacy of in vitro differentiated human embryonic stem (hES, MB03) cells expressing Nurr1 in relief of symptomatic motor behavior of Parkinson's disease (PD) animal models MB03 was genetically modified to express Nurr1 protein and was induced to differentiate according to 2-/4+ protocol using retinoic acid and ascorbic acid. The differentiation-induced cells were selected for 10 to 20 days thereafter in N2 medium. Upon selection, cells expressing GFAP, TH, or NF200 were 38.8%, 11%, and 20.5%, respectively. in order to examine therapeutic effects of the differentiated cells in PD animal model, rats were unilaterally lesioned by administration of 6-kydroxydopamine HCI (6-OHDA) into medial forebrain region (MFB, AP -4.4 mm, ML 1.2 mm, DV 78 mm with incision bar set at -2.4 mm), as a reference to bregma and the surface of the skull. Confirmation of successful lesion by apomorphine-induced rotational behavior, differentiated cells were transplanted into the striatum (AP 1.0, ML 3.5, DV -5.0; AP 0.6, ML 2.5, DV -4.5). Improvements of asymmetric motor behavior by the transplantation were examined every two weeks after the surgery. In two weeks, numbers of rotation by the experimental rats were $-14.8 \pm 33.9%$ (P<0.05) of the number before transplantation, however, the ratio increased slightly to $13.6 \pm 56.3%$ in six weeks. In contrast, the ratio of sham-grafted animals ranged from 112.3+8.5% to 139.2+28.9% during the examination. Immunohistochemical studies further confirmed the presence, survival, migration, and expression of TH of the transplanted human cells.

• Explosives and Blasting
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• v.29 no.2
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• pp.32-42
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• 2011

For blasting vibration analysis, there are mostly two methods, firstly, far field vibration analysis to estimate limit of building damage, secondly, near field subterranean vibration analysis to estimate rock damage and subterranean structure facilities. The former has been mainly used in our country and aboard, the latter is mostly analysed by using Homberg's model. In this model, basic input data are acquired in far field surface vibration. But in the consideration of the results of being conducted in this area over the past few decade, it is required that Homberg's model is modified. For the purpose of this, measurements of near field vibration were first conducted in our country. But it was only proposed the measurement method and the method of analysis or prediction was not suggested. Accordingly, in this paper, measurements of near field subterranean vibration were conducted and the method of analysis or prediction of near field subterranean vibration would be suggested.

• Journal of Environmental Science International
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• v.18 no.6
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• pp.609-619
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• 2009

SWAT-K model is a modified version of the original SWAT, and is known to more accurately estimate the streamflows and pollutant loadings in Korean watersheds. In this study, its hydrological components were compared with those of HSPF in order to analyse the differences in total runoff including evapotranspiration(ET), surface flow, lateral flow and groundwater flow from the Chungju Dam watershed during $2000{\sim}2006$. Averaged annual runoff with SWAT-K overestimated by 1%, and HSPF underestimated it by 3% than observed runoff. Determination coefficients($R^2$) for observed and simulated daily streamflows by both the models were relatively good(0.80 by SWAT-K and 0.82 by HSPF). Potential ET and actual ET by HSPF were lower in winter, but similar or higher than those by SWAT-K. And though there were some differences in lateral and groundwater flows by two models because of the differences in hydrological algorithms, the results were to be reasonable. From the results, it was suggested that we should utilize a proper model considering the characteristic of study area and purposes of the model application because the simulated results from same input data could be different with models used. Also we should develop a novel model appropriate to Korean watersheds by enhancing limitations of the existing models in the future.