• 한국동물위생학회지
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• 제27권2호
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• pp.133-137
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• 2004

This study was attempted to survey on the prevalence of canine heartworm(Dirofilaria immitis) infections among 206 dogs (male 92, female 114) in Daejeon area from March to December 2003. Blood samples taken from dogs were examined for the presence of D immitis microfilaria by the modified Knott's test and an antigen test( $AGEN^{TM}$ canine heartworm). The result obtained from this experiment were summarized as follows; 1. Eighteen(8.7%) of the 206 examined dogs were microfilaria positive, while twenty five dogs(12.1%) were antigen test positive. 2. The infection rates of heartworm in dogs at the age of <2, 2∼4 and $\geq$5 were 3.8%, 18% and 25%, respectively. 3. The infection rates of heartworm in dogs by housing of indoor and outdoor were 3.3% and 36.4%, respectively. 4. The regional infection rates were Dong-gu(13/29, 44.8%), Seo-gu(7/92, 7.6%), Yuseong-gu(2/33, 6.1%), Jung-gu(1/17, 5.9%) and Daedeok-gu(2/35, 5.7%). 5. The seasonal infection rates were summer(June∼August, 16.7%), autumn (September∼October, 14%), spring(March∼May, 9.4%) and winter(November∼December. 7.7%).7.7%).

• 대한수의학회지
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• 제40권1호
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• pp.130-137
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• 2000

In order to diagnose canine heartworm infection by antigen capture ELISA, the crude somatic(S), partial somatic(below 45kDa) and excretory/secretory(E/S) antigen of adult heartworm were identified and the antigenicity was examined by silver stain, immunoblot and ELISA. Then, production of monoclonal antibody to specific antigen carried out in this experiment. The bands to S antigen and E/S antigen were recognized between 10 and 200kDa and common bands were recognized strongly 14, 18, 28, 43kDa by silver stain. By western blot analysis, fractions to S antigen were recognized 14, 16, 18, 20, 24, 28, 32, 43, 50, 55kDa, etc. and only a 14kDa to E/S antigen in positive sera which were positive in modified Knott's test and necropsy. In ELISA, the positive sera reacted to antigens(SA, $SA_{45}$, E/S) were significantly different from negative sera by Student's t-test(p<0.05). Four hybridoma cell lines(14, 16, 17, 32kDa) than produce specific monoclonal antibodies for these antigens were obtained by immunizing BALB/c mice with a partially purified somatic antigen (below 45kDa) preparation, by fusing spleen cells with SP2/O cell myeloma cells, and by screening cell culture supernatants for antibody. In these results, it was confirmed that partial somatic antigen(below 45kDa) or E/S antigen can be used for serologic diagnosis of heartworm infection and monoclonal antibody reacting with specific antigen(14kDa) can be used for antigen capture ELISA in prepatent period of canine heartworm infection.

• 한국동물위생학회지
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• 제26권1호
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• pp.19-26
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• 2003

This study was attempted to survey on the prevalence of canine heartworm(Dirofilaria immitis) infection among 100 dogs(male 39, female 61) on the nine breeding farms in eastern Chungnam province in December 2002. Blood samples taken from dogs were examined for the presence of D immitis microfilaria by the modified Knott's test and an antigen test was using FASTest$\^$/ HW Antigne kit (Mega Cor A-6912 Horbranz-Austraia). 1. Eleven(11.0%) of the 100 examined dogs were microfilaria positive, while nineteen dogs(19.0%) were antigen positive, which suggested that the antigen test was more sensitive than the microfilarial test in detecting heartworm infection. 2. Infected dogs were observed higher more at 2 years older ages(4/48, 8.3%) and male(9/39, 23.1%) than young ages(4/48, 8.3%) and female(10/61, 16.4%). 3. The regional infection rates were of Gongju(15/43, 34.9%), Geumsan(4.27, 14.8%), while none of infection dogs in Yeongi(0/30, 0%). 4. Survey for hematological values of D immitis infected dogs : WBC and eosinophils were 21.4${\pm}$7.2 k/${\mu}\ell$, 3.5${\pm}$0.4 k/${\mu}\ell$, respectively. In conculsion, this study could be overemphasized the importance of control program the heartworm in eastern Chungnam province