• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.227-233
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• 2015

Two recombinant arabinosyl hydrolases, α-_L-arabinofuranosidase from Geobacillus sp. KCTC 3012 (GAFase) and endo-(1,5)-α-_L-arabinanase from Bacillus licheniformis DSM13 (BlABNase), were overexpressed in Escherichia coli, and their synergistic modes of action against sugar beet (branched) arabinan were investigated. Whereas GAFase hydrolyzed 35.9% of _L-arabinose residues from sugar beet (branched) arabinan, endo-action of BlABNase released only 0.5% of _L-arabinose owing to its extremely low accessibility towards branched arabinan. Interestingly, the simultaneous treatment of GAFase and BlABNase could liberate approximately 91.2% of _L-arabinose from arabinan, which was significantly higher than any single exo-enzyme treatment (35.9%) or even stepwise exo- after endo-enzyme treatment (75.5%). Based on their unique modes of action, both exo- and endo-arabinosyl hydrolases can work in concert to catalyze the hydrolysis of arabinan to _L-arabinose. At the early stage in arabinan degradation, exo-acting GAFase could remove the terminal arabinose branches to generate debranched arabinan, which could be successively hydrolyzed into arabinooligosaccharides via the endo-action of BlABNase. At the final stage, the simultaneous actions of exo- and endo-hydrolases could synergistically accelerate the _L-arabinose production with high conversion yield.

• International Journal of Concrete Structures and Materials
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• 제10권2호
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• pp.237-247
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• 2016

Progressive collapse resistance of RC buildings can be analyzed by considering column loss scenarios. Using finite element analysis and a static test, the progressive collapse process of a RC frame under monotonic vertical displacement of a side column was investigated, simulating a column removal scenario. A single-story 1/3 scale RC frame that comprises two spans and two bays was tested and computed, and downward displacement of a side column was placed until failure. Our study offers insight into the failure modes and progressive collapse behavior of a RC frame. It has been noted that the damage of structural members (beams and slabs) occurs only in the bay where the removal side column is located. Greater catenary action and tensile membrane action are mobilized in the frame beams and slabs, respectively, at large deformations, but they mainly happen in the direction where the frame beams and slabs are laterally restrained. Based on the experimental and computational results, the mechanism of progressive collapse resistance of RC frames at different stages was discussed further. With large deformations, a simplified calculation method for catenary action and tensile membrane action is proposed.

• Steel and Composite Structures
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• 제26권4호
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• pp.407-420
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• 2018

Experiments were performed to explore the hysteretic performance of steel reinforced concrete (SRC) cross-shaped columns. Nine specimens were designed and tested under the combined action of compression, flexure, shear and torsion. Torsion-bending ratio (i.e., 0, 0.14, 0.21) and steel forms (i.e., Solid - web steel, T - shaped steel, Channel steel) were considered in the test. Both failure processes and modes were obtained during the whole loading procedure. Based on experimental data, seismic indexes, such as bearing capacity, ductility and energy dissipation were investigated in detail. Experimental results suggest that depending on the torsion-bending ratio, failure modes of SRC cross-shaped columns are bending failure, flexure-torsion failure and torsion-shear failure. Shear - displacement hysteretic loops are fuller than torque - twist angle hysteretic curves. SRC cross-shaped columns exhibit good ductility and deformation capacity. In the range of test parameters, the existence of torque does not reduce the shear force but it reduces the displacement and bending energy dissipation capacity. What is more, the bending energy dissipation capacity increases with the rising of displacement level, while the torsion energy dissipation capacity decreases.

• 한국구조물진단유지관리공학회 논문집
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• 제10권6호
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• pp.113-123
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• 2006

사장교가 장경간으로 시공됨에 따라 대형화 되고 지진하중, 풍하중 및 차량하중 등 동적 하중에 의해 유발되는 진동현상에 취약한 단점이 나타난다. 이러한 하중 등에 의해 발생된 구조 손상은 구조물의 진동모드 특성에 영향을 미치게 된다. 기존의 정밀안전진단 기술을 이용하여 사장교의 구조 손상을 검색하고 평가하는 것은 상당한 비용과 시간이 소요될 뿐만 아니라 전체적인 구조거동 특성의 변화를 발견하기 어려울 것이다. 따라서 본 연구는 사장교에 대하여 구조손상 전의 진동모드 특성치와 구조손상 후의 진동모드 특성치를 이용하여 구조거동 특성의 변화를 검토하고 구조손상 검색을 수행하였다.

• Steel and Composite Structures
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• 제11권1호
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• pp.39-58
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• 2011

The subject of the ongoing research work is to analyze the composite action of the structural elements of composite slabs with profiled steel decking by experimental and numerical studies. The mechanical and frictional interlocks result in a complex behaviour and failure under horizontal shear action. This is why the design characteristics can be determined only by standardized experiments. The aim of the current research is to develop a computational method which can predict the behaviour of embossed mechanical bond under shear actions, in order to derive the design characteristics of composite slabs with profiled steel decking. In the first phase of the research a novel experimental analysis is completed on an individual concrete encased embossment of steel strip under shear action. The experimental behaviour modes and failure mechanisms are determined. In parallel with the tests a finite element model is developed to follow the ultimate behaviour of this type of embossment, assuming that the phenomenon is governed by the failure of the steel part. The model is verified and applied to analyse the effect of embossment's parameters on the behaviour. In the extended investigation different friction coefficients, plate thicknesses, heights and the size effects are studied. On the basis of the results the tendencies of the ultimate behaviour and resistance by the studied embossment's characteristics are concluded.

• 대한기계학회논문집A
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• 제21권7호
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• pp.1073-1080
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• 1997

The stage 1 compaction behavior of tool steel powder under die pressing was studied. The friction effects between the powder and the die wall under different die pressing modes were also investigated. The elastoplastic constitutive equations based on the yield functions by Fleck et al. and by Shima and Oyane were implemented into a finite element program to simulate die compaction processes. Finite element calculations were compared with experimental data for densification and density distribution of tool steel powder under single and double action die pressing. Finite element calculations using the yield function by Fleck et al. agreed better with experimental data than by Shima and Oyane.

• Nuclear Engineering and Technology
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• 제55권3호
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• pp.839-849
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• 2023

The development of automation technology to reduce human error by minimizing human intervention is accelerating with artificial intelligence and big data processing technology, even in the nuclear field. Among nuclear power plant operation modes, the startup and shutdown operations are still performed manually and thus have the potential for human error. As part of the development of an autonomous operation system for startup operation, this paper proposes an action coordinating strategy to obtain the optimal actions. The lower level of the system consists of operating blocks that are created by analyzing the operation tasks to achieve local goals through soft actor-critic algorithms. However, when multiple agents try to perform conflicting actions, a method is needed to coordinate them, and for this, an action coordination strategy was developed in this work as the upper level of the system. Three quantification methods were compared and evaluated based on the future plant state predicted by plant parameter prediction models using long short-term memory networks. Results confirmed that the optimal action to satisfy the limiting conditions for operation can be selected by coordinating the action sets. It is expected that this methodology can be generalized through future research.

• Earthquakes and Structures
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• 제27권3호
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• pp.177-189
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• 2024

Accurately predicting the failure modes of reinforced concrete (RC) columns is essential for structural design and assessment. In this study, the challenges of imbalanced datasets and complex feature selection in machine learning (ML) methods were addressed through an optimized ML approach. By combining feature selection and oversampling techniques, the prediction of seismic failure modes in rectangular RC columns was improved. Two feature selection methods were used to identify six input parameters. To tackle class imbalance, the Borderline-SMOTE1 algorithm was employed, enhancing the learning capabilities of the models for minority classes. Eight ML algorithms were trained and fine-tuned using k-fold shuffle split cross-validation and grid search. The results showed that the artificial neural network model achieved 96.77% accuracy, while k-nearest neighbor, support vector machine, and random forest models each achieved 95.16% accuracy. The balanced dataset led to significant improvements, particularly in predicting the flexure-shear failure mode, with accuracy increasing by 6%, recall by 8%, and F1 scores by 7%. The use of the Borderline-SMOTE1 algorithm significantly improved the recognition of samples at failure mode boundaries, enhancing the classification performance of models like k-nearest neighbor and decision tree, which are highly sensitive to data distribution and decision boundaries. This method effectively addressed class imbalance and selected relevant features without requiring complex simulations like traditional methods, proving applicable for discerning failure modes in various concrete members under seismic action.

• Wind and Structures
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• 제9권5호
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• pp.397-412
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• 2006

This paper presents the wind excited acceleration responses of a 50 m guyed mast under the action of Typhoon Dujuan. The response of the structure is reconstructed from using a full finite element model and an equivalent beam-column model. The wind load is modelled based on the measured wind speed and recommendations for high-rise structures. The nonlinear time response analysis is conducted using the Newton Raphson iteration procedure. Comparative studies on the measured and computed frequencies and acceleration responses show that the torsional vibration of the structure is significant particularly in the higher vibration modes after the first few bending modes. The equivalent model, in general, gives less accurate amplitude predictions than the full model because of the omission of torsional stiffness of the mast in the vibration analysis, but the root-mean-square value is close to the measured value in general with an error of less than 10%.

• Applied Biological Chemistry
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• 제35권6호
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• pp.462-469
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• 1992

대복피(빈랑나무의 과피)로부터 면역조절 역할이 기대되는 2종의 보체활성화(항보체) 다당을 정제하고 그 작용양식에 대해 검토하였다. 활성다당 AC-2-IIIa 및 AC-2-IIIc는 농도가 증가함에 따라 항보체 활성이 증가하는 양상을 보였으며, 2가 금속이온 부재시 대조구에 비해 거의 완전한 활성의 감소를 보인 반면, $Ca^{2+}$ 이온만을 선택적으로 제거한 경우 활성이 상당량 유지되었다. 또한 이들 활성다당들은 비교적 강력한 보체 제 2경로 활성화능을 나타내었으며, $Ca^{2+}$ 이온 부재 상태에서 정상인의 혈청과 반응시, C3의 분해산물을 anti-human C3를 이용한 면역 전기영동법에 의해 확인할 수 있었다. 또한 anti-human whole serum를 이용한 면역 전기영동 결과, ${\alpha}2-M$ peak 대비 3rd peak의 높이 비율은 AC-2-IIIa가 $1.50{\pm}0.04$, AC-2-IIIc가 $1.22{\pm}0.08$이었는바, 이러한 사실로부터 대복피 유래 AC-2-IIIa 및 AC-2-IIIc의 보체 활성화 양식은 classical 및 alternative pathway 양경로를 모두 경유함을 알 수 있었다.