• 한국농림기상학회지
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• 제20권2호
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• pp.228-232
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• 2018

기후변화 영향평가를 위하여 프로세스 작물모형이 많이 이용되고 있지만, FORTRAN, C++, Delphi, Java와 같은 컴퓨터 프로그래밍 언어로 만들어지기 때문에 농학자들이 작물 모형을 제작하는 것이 쉽지 않다. 배추 모형을 개발하기 위해 6 가지 온도 체계를 가진 토양-식물-대기 연구(SPAR) 실험 자료가 사용되었다. SPAR 챔버에서의 식물 재배 기간 동안 잎의 수, 잎의 면적, 식물의 생장률을 6 회 측정 하였다. 또한 휴대용 LI-6400 광합성 측정기를 이용하여 잎의 광합성을 측정 하였다. 잎 수준 광합성 예측은 Farquhar, von Caemmerer 및 Berry (FvCB) 모형을 적용 하였고, 수관의 광합성은 Sun/Shade 모형이 사용되었다. 이러한 전 과정은 BuildIt 이라는 Excel 추가기능이 포함된 엑셀 파일로 제작되었다. 개발된 모형으로 시간 단위의 기상 입력 자료를 사용하여 배추의 광합성, 생장률 및 기타 생리 변수의 변화를 모의할 수 있었으며, 측정된 배추의 건조 중량의 변화와 모형에서 예측된 동화량과는 비례적인 관계를 나타내었으나, 온도에 따라서 다르게 나타났다.

• 한국토양비료학회지
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• 제49권5호
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• pp.420-428
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• 2016

For more than 50 years, satellite images have been used to monitor crop growth. Currently, unmanned aerial vehicle (UAV) imagery is being assessed for analyzing within field spatial variability for agricultural precision management, because UAV imagery may be acquired quickly during critical periods of rapid crop growth. This study refers to the derivation of growth estimating equation for highland Kimchi cabbage using UAV derived normalized difference vegetation index (NDVI) and agro-meteorological factors. Anbandeok area in Gangneung, Gangwon-do, Korea is one of main districts producing highland Kimchi cabbage. UAV imagery was taken in the Anbandeok ten times from early June to early September. Meanwhile, three plant growth parameters, plant height (P.H.), leaf length (L.L.) and outer leaf number (L.N.), were measured for about 40 plants (ten plants per plot) for each ground survey. Six agro-meteorological factors include average temperature; maximum temperature; minimum temperature; accumulated temperature; rainfall and irradiation during growth period. The multiple linear regression models were suggested by using stepwise regression in the extraction of independent variables. As a result, $NDVI_{UAV}$ and rainfall in the model explain 93% of the P.H. and L.L. with a root mean square error (RMSE) of 2.22, 1.90 cm. And $NDVI_{UAV}$ and accumulated temperature in the model explain 86% of the L.N. with a RMSE of 4.29. These lead to the result that the characteristics of variations in highland Kimchi cabbage growth according to $NDVI_{UAV}$ and other agro-meteorological factors were well reflected in the model.

• The Plant Pathology Journal
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• 제36권1호
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• pp.54-66
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• 2020

This study was conducted to evaluate usefulness of numerical weather prediction data generated by the Unified Model (UM) for plant disease forecast. Using the UM06- and UM18-predicted weather data, which were released at 0600 and 1800 Universal Time Coordinated (UTC), respectively, by the Korea Meteorological Administration (KMA), disease forecast on bacterial grain rot (BGR) of rice was examined as compared with the model output based on the automated weather stations (AWS)-observed weather data. We analyzed performance of BGRcast based on the UM-predicted and the AWS-observed daily minimum temperature and average relative humidity in 2014 and 2015 from 29 locations representing major rice growing areas in Korea using regression analysis and two-way contingency table analysis. Temporal changes in weather conduciveness at two locations in 2014 were also analyzed with regard to daily weather conduciveness (C_i) and the 20-day and 7-day moving averages of C_i for the inoculum build-up phase (C_inc) prior to the panicle emergence of rice plants and the infection phase (C_inf) during the heading stage of rice plants, respectively. Based on C_inc and C_inf, we were able to obtain the same disease warnings at all locations regardless of the sources of weather data. In conclusion, the numerical weather prediction data from KMA could be reliable to apply as input data for plant disease forecast models. Weather prediction data would facilitate applications of weather-driven disease models for better disease management. Crop growers would have better options for disease control including both protective and curative measures when weather prediction data are used for disease warning.

• 한국약용작물학회지
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• 제13권2호
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• pp.91-94
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• 2005

Plants have been known to accumulate a very diverse range of triterpene saponins. We have investigated the regulation of saponin biosynthesis in higher plants using Centella asiatica (L.) Urban as a model plant. Effects of a feeding precursor on asiaticoside production from leaves and on the level of two-type OSCs mRNA were investigated. As a feeding precursor, squalene negatively affected the levels of CYS and bAS mRNA, but it also decreased the production of asiaticoside from whole plants. Plant hormones regulate secondary metabolism, and in plant tissue cultures they could affect both culture growth and secondary metabolite production. Although enhancement of asiaticoside production from whole plant cultures by addition of TDZ (thidiazuron) has been reported, the positive effect of TDZ on the levels of OSCs transcripts was not observed.

• The Plant Pathology Journal
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• 제25권4호
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• pp.389-399
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• 2009

A group of beneficial plant bacteria has been shown to increase crop growth referring to as plant growth-promoting rhizobacteria (PGPR). PGPR can decrease plant disease directly, through the production of antagonistic compounds, and indirectly, through the elicitation of a plant defense response termed induced systemic resistance (ISR). While the mechanism of PGPR-elicited ISR has been studied extensively in the model plant Arabidopsis, it is less well characterized in crop plants such as pepper. In an effort to better understand the mechanism of ISR in crop plants, we investigated the induction of ISR by Bacillus cereus strain BS107 against Xanthomonas axonopodis pv. vesicatoria in pepper leaves. We focused on the priming effect of B. cereus strain BS107 on plant defense genes as an ISR mechanism. Of ten known pepper defense genes that were previously reported to be involved in pathogen defense signaling, the expression of Capsicum annum pathogenesis-protein 4 and CaPR1 was systemically primed by the application of strain BS107 onto pepper roots confirming by quantitative-reverse transcriptase PCR. Our results provide novel genetic evidence of the priming effect of a rhizobacterium on the expression of pepper defense genes involved in ISR.

• 한국작물학회지
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• 제58권4호
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• pp.336-346
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• 2013

Amaranth (Amaranthus sp. L.) is an important group of plants that includes grain, vegetable, and ornamental types. Centers of diversity for Amaranths are Central and South America, India, and South East Asia, with secondary centers of diversity in West and East Africa. The present study was performed to determine the genetic diversity and population structure of 75 amaranth accessions: 65 from South America and 10 from South Asia as controls using 14 SSR markers. Ninety-nine alleles were detected at an average of seven alleles per SSR locus. Model-based structure analysis revealed the presence of two subpopulations and 3 admixtures, which was consistent with clustering based on the genetic distance. The average major allele frequency and polymorphic information content (PIC) were 0.42 and 0.39, respectively. According to the model-based structure analysis based on genetic distance, 75 accessions (96%) were classified into two clusters, and only three accessions (4%) were admixtures. Cluster 1 had a higher allele number and PIC values than Cluster 2. Model-based structure analysis revealed the presence of two subpopulations and three admixtures in the 75 accessions. The results of this study provide effective information for future germplasm conservation and improvement programs in Amaranthus.

• Plant Biotechnology Reports
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• 제2권3호
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• pp.199-206
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• 2008

We have developed an efficient system of assessing the ability of a gene silencing cassette to silence transcripts from recalcitrant or poorly studied plant species by using a model plant as a host for the gene of interest. Tobacco plants transgenic for Lachrymatory Factor Synthase (LFS) enzyme activity from onion were first produced by introducing a CaMV 35S-onion-lfs gene construct. These plants were then subjected to a second transformation with an RNAi construct directed against the lfs gene sequence. LFS enzyme activity assay showed that the transgenic plants, containing both the lfs gene and the RNAi construct, had significantly reduced LFS activity. This observation was supported by Western analysis for the LFS protein and further validated by quantitative RT-PCR analysis that demonstrated a significant reduction in the lfs transcript level in the dual transformants. In this work, we have demonstrated that the RNAi construct is a suitable candidate for the development of a non-lachrymatory onion. Our model plant RNAi system has wide-reaching applications for assessment and targeting of plant secondary pathway genes, from poorly studied or recalcitrant plant species, that are important in the pharmacological, food and process industries.

• 한국육종학회지
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• 제41권3호
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• pp.252-260
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• 2009

1. 본 연구에서는 공동배양 배지에 Agrobacterium 성장 억제물질인 silver nitrate를 첨가하고 변온과 여과지처리를 추가하여 공동배양 기간을 7일로 늘였으며, 또한 항산화 물질 3종을 공동배양 배지에 첨가하여 세포의 oxidative burst를 최소화함으로써 벼 형질전환효율을 높일 수 있었다. 또한 이 방법을 적용하여 형질전환이 어려운 품종을 대상으로도 형질전환 식물체를 작성할 수 있었다. 2. 벼 형질전환체의 70%에서 도입유전자 수가 1copy인 것으로 나타나, 적은 수의 유전자가 안정적으로 도입됨을 확인 하였다. 3. 이러한 결과를 바탕으로, 새로운 공동배양 방법을 사용하여 우수한 농업적 형질을 가진 벼 육종 소재 및 품종을 신속하게 개발할 수 있을 것으로 기대된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.60-60
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• 2017

It is important to measure the gas exchange activity of the crops in canopy scale to understand the process of biomass production and yield formation. Thermal imaging of the canopy surface temperature is a powerful tool to detect the gas exchange activity of the crop canopy. The simultaneous measurement of the canopy temperature and the meteorological data enables us to calculate the canopy diffusive conductance ($g_c$) based on the heat flux model (Monteith et al. 1973, Horie et al. 2006). It is, however, difficult to realize the long-term and continuous monitoring of $g_c$ due to the occurrence of the calculation error caused by the fluctuation of the environmental condition. This is partly because the model assumption is too simple to describe the meteorological and aerodynamic conditions of the crop canopy in the field condition. Here we report the novel method of the direct measurement of the aerodynamic resistance ($r_a$) of the crop canopy, which enables us the stable and continuous measurement of the gas exchange capacity of the crop plants. The modified heat balance model shows the improved performance to quantify $g_c$ under the fluctuating meteorological condition in the field. The relationship between $g_c$ and biomass production of rice and soybean varieties is also discussed in the presentation.

• Journal of Ginseng Research
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• 제38권1호
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• pp.73-77
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• 2014

Field and nutrient cultures of American ginseng (Panax quinquefolius L.) were used to establish foliar symptoms related to boron (B) concentration in leaves and soils, and to evaluate radish as a time-saving model system for B nutrition. Application of excess B, 8 kg/ha versus the recommended 1.5 kg/ha, to field plantings of 2-, 3-, and 4-yr-old American ginseng plants just prior to crop emergence caused, within 4 wk after crop emergence, leaf symptoms of chlorosis followed by necrosis starting at the tips and progressing along the margins. The B concentration in leaves of 2-4-yr-old plants receiving 1.5 kg/ha Bwas $30{\mu}g/g$ dry mass compared to $460{\mu}g/g$ dry mass where 8 kg/ha B was applied. Similarly, B concentration in soils receiving the lower B concentration was 1.8 mg/g dry mass and $2.2-2.8{\mu}g/g$ dry mass where the higher B concentration was applied. Application of 8 kg/ha B reduced the dry yield of 3rd-yr roots by 20% from 2745 kg/ha to 2196 kg/ha and 4th-yr roots by 26% from 4130 kg/ha to 3071 kg/ha. Ginseng seedlings and radish were grown under greenhouse conditions in nutrient culture with four B concentrations ranging from 0 mg/L to 10 mg/L. At 5 mg/L and 10 mg/L ginseng and radish developed typical leaf B toxicity symptoms similar to those described above for field-grown plants. Increasing B in the nutrient solution from 0.5 mg/L to 10 mg/L decreased, in a linear fashion, the root and leaf dry mass of ginseng, but not radish. Given the many similarities of ginseng and radish to B utilization, radish might be used as a timesaving model system for the study of B, and other micronutrients, in the slow-growing perennial ginseng.