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Comparative evaluation of hyaluronic acid-based dressing versus hydrocolloid dressing in rat dermal wound healing

  • Hye Mi Lee;Eun Jung Jang;Ki Hun Choi;Young Cheon Na
    • Archives of Craniofacial Surgery
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    • v.25 no.5
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    • pp.224-229
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    • 2024
  • Background: Wound healing is a complex process influenced by a variety of environmental factors. Dressing materials play a critical role in creating barriers against contaminants, maintaining optimal moisture levels, and absorbing wound exudate. Therefore, selecting materials tailored to wound characteristics is crucial for enhancing outcomes. Hyaluronic acid (HA) is a natural biocompatible polymer that supports healing by regulating inflammation and promoting tissue repair. This study compared HA- and hydrocolloid-based hydrogels in a rat model to optimize wound care strategies. Methods: Full-thickness dermal wounds (diameter, 8 mm) were created on the dorsal skin of 12 Sprague-Dawley rats under sevoflurane anesthesia. The wounds were treated with HA/silver sulfadiazine gel (group A), hydrocolloid gel (group B), or left untreated (control), all covered with a transparent dressing. Biopsy specimens on days 3, 7, and 21 were used to assess histological parameters: inflammatory cell infiltration, fibroblast infiltration, collagen deposition, neovascularization, and epithelial thickness, using a semi-quantitative scoring system. Histological analyses were conducted blindly, and statistical analyses were performed using the Kruskal-Wallis test (p< 0.05). Results: On day 3, group A showed significantly higher inflammatory cell infiltration and collagen deposition than other groups, indicating extracellular matrix formation. By day 7, angiogenesis was highest in group A, followed by group B and controls. By day 21, all wounds had completely healed. Epithelial layer thickness, reflecting inflammation and fibroblast maturity, was significantly higher in group A. Conclusion: This study compared HA-based hydrogel and hydrocolloid-based dressings through histological analyses to elucidate wound healing mechanics. HA-based hydrogel dressings significantly enhanced wound recovery. However, generalizing these outcomes requires future studies to expand the range of effective wound treatment materials. These findings underscore the potential of HA-based dressings to enhance clinical outcomes in wound management, suggesting avenues for improving therapeutic strategies.

Development of Ecological Restoration Model Consider Analysis on the Vegetation Structure of Burned Area (산불지역 식생구조 분석을 통한 식생복원 모델 개발)

  • Kim, Jeong-Ho;Lee, Soo-Dong
    • Korean Journal of Environment and Ecology
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    • v.21 no.5
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    • pp.400-414
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    • 2007
  • This study has analyzed the vegetation structure to suggest a vegetation ecological restoration model by using the case of the afforestation for erosion control area with Pinus koreaiensis and Betula platyphylla, etc., on the hills of the Young-in mountains after its great fire in 2000. Of the area having a dimension of $1,152,404.3m^2$ selected as a survey site for the existing vegetation, the forest fire area accounted for 69.2% among which, brushwoods accounted the most for 24.67%. As a result of analysis of the 27 surveyed unit plots[unit dimension: $100m^2$] set up in consideration of the existing vegetation pattern and damaged state from the forest fire, the surveyed area was classified into 10 communities. Shrub layer's vegetation was found to be dominant in forest fire areas and the surveyed sites were classified into 5 plant communities, i.e. P. koraiensis community, Quercus variabilis community, P. thunbergii community, Q. serrata community, B. platyphylla community in forest fire areas, while non-forest fire areas were classified into 5 plant communities, such as P. densiflora community, Q. acutissima community, Q. serrata community, Q. mongolica-Q. serrata community, B. platyphylla community. Species diversity of forest fire areas was $0.3679{\sim}0.5907$ and that of non-forest fire areas was $0.5728{\sim}0.8865$. In addition, the number of the species in the forest fire areas was $5{\sim}8$ and that of non-forest areas was $8{\sim}12$; however, the population of forest fire areas$(156{\sim}456)$ was higher than that of non-forest fire areas$(61{\sim}227)$. In the analysis of growth density per layer$[of\;100m^2]$, there appeared $1{\sim}8$ trees of Q. mongolica and $3{\sim}5$ trees of Q. serrata in the upper layer species; $2{\sim}4$ trees of Q. serrata and one tree of Q. mongolica in the canopy layer. As for the characteristics of soil, acidity of forest fire areas was pH 5.45 and that of non-forest fire was pH 5.25. By setting up the middle D.B.H range of Q. mongolica-Q. serrata community as the vegetation restoration model, planting species, planting density and planting models are suggested.

Effect of Temperature and Various Pre-treatments on Germination of Hippophae rhamnoides Seeds (갈매보리수나무 종자의 온도 및 여러 가지 전처리에 따른 발아반응)

  • Choi, Chung-Ho
    • Korean Journal of Plant Resources
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    • v.25 no.1
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    • pp.132-141
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    • 2012
  • This study was carried out to test seed germination responses to temperatures and pre-treatments in Hippophae rhamnoides, which has many abilities in antioxidant activity, soil improvement and erosion control. H. rhamnoides seeds were placed at 10, 15, 20, 25, 30 and $35^{\circ}C$ under light condition. As the results, germination percentage (GP) was the highest at 15 and $20^{\circ}C$, and mean germination time (MGT), germination rate (GR) and germination value (GV) were the highest at $25^{\circ}C$. Quadratic and linear regression model were used to determine the cardinal temperatures such as base ($T_b$), maximum ($T_m$) and optimum ($T_o$) temperature for germination. In quadratic regression model using PG, $T_b$, $T_m$ and $T_o$ was estimated as 0.6, 36.4 and $18.5^{\circ}C$, respectively, and temperature range for germination was $35.8^{\circ}C$. In linear regression model using GR, $T_b$, $T_m$ and $T_o$ was estimated as 8.3, 35.4 and $25.3^{\circ}C$, respectively, and temperature range for germination was $27.2^{\circ}C$. Germination properties were investigated after H. rhamnoides seeds were treated by prechilling (1, 2, 4, 6 and 8 weeks), stratification (2, 4, 6 and 8 weeks), solid matrix priming (seed : carrier : water = 5 : 1 : 7, 8, 9 and 10), osmo-priming (-0.25, -0.5, -1.0 and -1.5 MPa) and calcium chloride ($CaCl_2$) -priming (100, 200, 300 and 400 mM). The highest GP was observed in $CaCl_2$ 300 and 400 mM treatments, and MGT was the shortest in stratification 6 and 8 weeks treatments. GR and GV were the highest and GP was the second highest when seeds were prechilled for 1 and 2 weeks. Consequently, prechilling 1 or 2 weeks treatment was considered as the appropriate method when we contemplate qualitative and quantitative effects in seedling production.

The Experimental Study on the Immuno-regulatory effect of ASARI HERBA CUM RADICE Herbal- acupuncture at Pyesu(BL13) on OVA-induced asthma in mice (폐유(肺兪) 세신약침(細辛藥鍼)이 OVA-induced Asthma Mouse Model의 면역조절작용(免疫調節作用)에 미치는 영향)

  • Ryu, Eun-sang;Lee, Hyun;Lee, Byung-ryul
    • Journal of Acupuncture Research
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    • v.22 no.3
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    • pp.185-200
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    • 2005
  • Objective : The aim of this study was to investigate the asthma-suppressive and immune-regulatory effect of AHCR-HA(ASARI HERBA CUM RADICE Herbal-acupuncture) at Pyesu(BLl3) on OVA(ovalbumin)-induced asthma in mice. Methods : C57BL/6 mice out of all the experimental groups, except the Normal group and the AHCR-HA group, were sensitized and challenged with OVA The mice in the AHCR-HA group and the OVA-AHCR-HA group were treated with AHCR-HA(1%) at Pyesu(BL13). The mice in the OVA-Saline group were injected with saline at Pyesu(BL13). The mice in the OVA-Needle-Prick group were treated with a single prick with an injection needle at Pyesu(BL13). AHCR-HA saline injection and needle prick were administered for 8 weeks, three times a week. Result : 1. The populations of granulocytes, CD3e-/CCR3+ cells, CD69+/CD3e+ cells, CD4+ cells and CD23+/B220+ cells in the OVA-induced asthmatic mouse lungs decreased significantly by AHCR-HA. 2. The lung weight, total cells in lung, total leukocytes in BALF, eosinophils in BALF, collagen accumulation in the lung sections of the OVA-AHCR-HA group decreased significantly. 3.The concentrations of IL-4, IL-5, IL-13, IgE in BALF and serum of the OVA-AHCR-HA group decreased significantly. 4. The numbers of Gr-1+/CD11b+, CCR3+, CD3e+, CD19+, CD3e+/CD69+cells in the OVA-AHCR-HA group decreased significantly. 5. The mRAN expressions of $TNF-{\alpha}$, IL-5, IL-4 and IL-13 in lung of the OVA-AHCR-HA group decreased significantly. 6. The AHCR-HA group didn't show any considerable difference from the Normal group. The OVA-saline group and the OVA-Needle prick group showed suppressive effects on OVA-induced asthma however they were not statistically significant. Conclusion : These results suggest that AHCR-HA at Pyesu(BL13) is considered to be effective in treating asthma and to be put to practical use in the future asthma clinic.

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Development of Java/VRML-based 3D GIS's Framework and Its Prototype Model (Java/VRML기반 3차원 GIS의 기본 구조와 프로토타입 모델 개발)

  • Kim, Kyong-Ho;Lee, Ki-Won;Lee, Jong-Hun
    • Journal of Korean Society for Geospatial Information Science
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    • v.6 no.1 s.11
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    • pp.11-17
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    • 1998
  • Recently, 3D GIS based on 3D geo-processing methodology and Internet environment are emerging issues in GIS fields. To design and implement 3D GIS, the strategic linkage of Java and VRML is first regarded: 3D feature format definition in the passion of conventional GIS including aspatial attributes, 3B feature indexing, 3D analytical operators such as selection, buffering, and Near, Metric operation such as distance measurement and statistical description, and 3D visualization. In 3D feature format definition, the following aspects are implemented: spatial information for 3D primitives extended from 2D primitives, multimedia data, object texture or color of VRML specification. DXF-format GIS layers with additional attributes are converted to 3D feature format and imported into this system. While, 3D analytical operators are realized in the form of 3D buffering with respect to user-defined point, line, polygon, and 3D objects, and 3D Near functions; furthermore, 'Lantern operator' is newly introduced in this 3D GIS. Because this system is implemented by Java applet, any client with Java-enable browser including VRML browser plug-in can utilize the new style of 3D GIS function in the virtual space. Conclusively, we present prototype of WWW-based 3D GIS, and this approach will be contribute to development of core modules on the stage of concept establishment and of real application model in future.

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Characteristics of Mass Transport Depending on the Feature of Tidal Creek at Han River Estuary, Gyeong-gi Bay, South Korea (경기만 염하수로에서의 비정규 격자 수치모델링을 통한 조간대 조수로의 고려에 따른 Mass Transport 특성)

  • Kim, Minha;Woo, Seung-Buhm
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.25 no.2
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    • pp.41-51
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    • 2013
  • The tidal creek dependent mass transport characteristic in Gyeong-Gi Bay (west coast of Korea) was studied using field measured data and numerical model. Gyeong-Gi Bay consists of 3 main tidal channels and contains a well-developed vast tidal flat. This region is famous for its large tidal difference and strong current. We aim to study the effect of tidal creek in the tidal flat on the mass exchange between the estuary and the ocean. For numerical application, the application of unstructured grid feature is essential, since the tidal creek has complicated shape and form. For this purpose, the FVCOM is applied to the study area and simulation is performed for 2 different cases. In case A, geographic characteristics of the tidal creek is ignored in the numerical grid and in case B, the tidal creek are constructed using unstructured grid. And these 2 cases are compared with the field measured cross-channel mass transport data. The cross-channel mass transport at the Yeomha waterway mouth and Incheon harbor was measured in June, 9~10 (Spring tide) and 17~18 (Neap tide), 2009. CTD casting and ADCP cross-channel transect was conducted 13 times in one tidal cycle. The observation data analysis results showed that mass transport has characteristic of the ebb dominance Line 1 (Yeomha waterway mouth), on the other hand, a flood dominant characteristic is shown in Line 2 (Incheon harbor front). By comparing the numerical model (case A & B) with observation data, we found that the case B results show much better agreement with measurement data than case A. It is showed that the geographic feature of tidal creek should be considered in grid design of numerical model in order to understand the mass transport characteristics over large tidal flat area.

Assessment of Climate Change Impact on Storage Behavior of Chungju and the Regulation Dams Using SWAT Model (SWAT을 이용한 기후변화가 충주댐 및 조정지댐 저수량에 미치는 영향 평가)

  • Jeong, Hyeon Gyo;Kim, Seong-Joon;Ha, Rim
    • Journal of Korea Water Resources Association
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    • v.46 no.12
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    • pp.1235-1247
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    • 2013
  • This study is to evaluate the climate change impact on future storage behavior of Chungju dam($2,750{\times}10^6m^3$) and the regulation dam($30{\times}10^6m^3$) using SWAT(Soil Water Assessment Tool) model. Using 9 years data (2002~2010), the SWAT was calibrated and validated for streamflow at three locations with 0.73 average Nash-Sutcliffe model Efficiency (NSE) and for two reservoir water levels with 0.86 NSE respectively. For future evaluation, the HadCM3 of GCMs (General Circulation Models) data by scenarios of SRES (Special Report on Emission Scenarios) A2 and B1 of the IPCC (Intergovernmental Panel on Climate Change) were adopted. The monthly temperature and precipitation data (2007~2099) were spatially corrected using 30 years (1977~2006, baseline period) of ground measured data through bias-correction, and temporally downscaled by Change Factor (CF) statistical method. For two periods; 2040s (2031~2050), 2080s (2071~2099), the future annual temperature were predicted to change $+0.9^{\circ}C$ in 2040s and $+4.0^{\circ}C$ in 2080s, and annual precipitation increased 9.6% in 2040s and 20.7% in 2080s respectively. The future watershed evapotranspiration increased up to 15.3% and the soil moisture decreased maximum 2.8% compared to baseline (2002~2010) condition. Under the future dam release condition of 9 years average (2002~2010) for each dam, the yearly dam inflow increased maximum 21.1% for most period except autumn. By the decrease of dam inflow in future autumn, the future dam storage could not recover to the full water level at the end of the year by the present dam release pattern. For the future flood and drought years, the temporal variation of dam storage became more unstable as it needs careful downward and upward management of dam storage respectively. Thus it is necessary to adjust the dam release pattern for climate change adaptation.

Population Dynamics of Korean Chub(Zacco koreanus, Cyprinidae) in the Upstream and Downstream of Lake Hoengseong (횡성호 상.하류에 분포하는 참갈겨니(Zacco koreanus, Cyprinidae)의 개체군 동태)

  • Choi, Jae-Seok;Park, Seung-Chul;Jang, Young-Su;Lee, Kwang-Yeol;Choi, Jun-Kil
    • Korean Journal of Environment and Ecology
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    • v.20 no.4
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    • pp.391-399
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    • 2006
  • The population dynamics of Zacco koreanus in the upstream and downstream of Hoengseong Lake, Korea were investigated from April to October 2005. As the result of a comparison of variations regarding the time of I koreanus populations, it was confirmed that downstream was a place to remain compared to upstream Also the b value, assessed by Length-weight relationship in upstream and downstream, were $3.21{\sim}3.35,\;and\;2.94{\sim}3.37$, respectively indicating the fish in downstream had better than upstream. Additionaly condition factor(K) of each population in upstream and downstream were $0.0292{\sim}0.0693\;and\;-0.0165{\sim}0.0499$, respectively, K value in upstream lower than downstream. On the other hand, the maximum-growth value$(L_{\infty})$ calculated by von Bertalanffy's growth model in upstream and downstream were 279.7mm and 303.9mm, respectively, indicating that the potential-growth ability of population in downstream was higher than population in upstream. Therefore, Z. koreanus population in upstream became worse based on matters of age structure, Length-weight relationship, and von Bertalanffy's growth model. These results are considered to show the change of the physical environment by the constructing of Hoengseong Dam.

A THREE DIMEMSIONAL PHOTOELASTIC STRESS ANALYSIS OF IMPLANT SUPPORTING BONE TISSUE ACCORDING TO DESIGN OF ATTACHMENTS USED FOR MANDIBULAR OVERDENTURE USING TWO OSSEOINTEGRATED IMPLANSTS (두개의 골유착성 임프란트를 이용한 하악 OVERDENTURE에서 ATTACHMENT 설계에 따른 임프란트 지지조직의 삼차원적 광탄성 응력분석)

  • Shin, Kyoo-Hag;Jeong, Chang-Mo;Jeon, Young-Chan;Hwang, Hie-Seong
    • The Journal of Korean Academy of Prosthodontics
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    • v.34 no.1
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    • pp.31-69
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    • 1996
  • The purpose of this investigation was to analyze stress distribution in implant supporting tissue according to different types of attachments such as combination bar attachment, Hader bar attachment, O-Ring attachment and Dal-Ro attachment that are used in mandibular overdenture by using two osseointegrated implants, to study the influence that POM IMC used in bar type attachment has in implant supporting tissue and compare the preceding analyses to find out an effective stress distribution method. Three dimensional photoelastic method was used to obtain the following results. (A) Analysis of stress distribution according to attachment type 1. Under vertical load condition, compressive stress was seen at implant supporting area of working side on all the photoelastic models but in Hader bar attachment tensional stress was seen at distal upper area of implant supporting area. Relatively Hader bar and O-Ring attachment showed even stress distribution pattern. 2. Under vertical load condition, compressive stress at implant apex area and tensional stress at implant lateral supporting area were seen at nonworking side of all models. 3. Under $25^{\circ}$ lateral load condition, general compressive stress was seen at working side implant supporting area in most of the models, especially at distal upper supporting area higher compressive stress concentration was seen in combination bar attachment and tensional stress concentration, in Hader bar attachment. 4. Under $25^{\circ}$ lateral load condition, compressive stress at implant apex area and tensional stress at implant lateral supporting area were seen at nonworking side of all models, except O-Ring model which showed compressive stress only. (B) Influence of POM IMC to stress distribution in bar type attachment 5. Under vertical load condition, better stress distribution pattern was seen at working side of combination bar and Hader bar attachment model using POM IMC. 6. Under vertical load condition, stress value was increased at nonworking side of combination bar attachment model using POM IMC and tendency of increasing compression was seen at nonworking side of Hader bar attachment model using POM IMC. 7. Under $25^{\circ}$ lateral load condition, better stress distribution pattern was seen at working side of combination bar attachment model using POM IMC but tendency of increasing stress was seen on working side of Hader bar attachment model using POM IMC. 8. Under $25^{\circ}$ lateral load condition, stress reduction was seen at nonworking side of combination bar attachment model using POM IMC but tendency of increasing stress was seen at nonworking side of Hader bar attachment model using POM IMC.

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Cryosurgery of Lung with 2.4 mm Cryoprobe: An Experimental in vivo Study of the Cryosurgery in Canine Model (냉동침을 이용한 폐 냉동수술의 동물실험: 냉동수술 방법의 비교 실험)

  • Kim Kwang-Taik;Chung Bong-Kyu;Lee Sung-Ho;Cho Jong-Ho;Son Ho-Sung;Fang Young-Ho;Sun Kyung;Park Sung-Min
    • Journal of Chest Surgery
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    • v.39 no.7 s.264
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    • pp.520-526
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    • 2006
  • Background: The clinical application of cryosurgery the management of lung cancer is limited because the response of lung at low temperature is not well understood. The purpose of this study is to investigate the response of the pulmonary tissue at extreme low temperature. Material and Method: After general anesthesia the lungs of twelve Mongrel dogs were exposed through the fifth intercostal space. Cryosurgical probe (Galil Medical, Israel) with diameter 2.4 mm were placed into the lung 20 mm deep and four thermosensors (T1-4) were inserted at 5 mm intervals from the cryoprobe. The animals were divided into group A (n=8) and group B (n=4). In group A the temperature of the cryoprobe was decreased to $-120^{\circ}C$ and maintained for 20 minutes. After 5 minutes of thawing this freezing cycle was repeated. In group B same freezing temperature was maintained for 40 minutes continuously without thawing. The lungs were removed for microscopic examination on f day after the cryosurgery. In four dogs of the group A the lung was removed 7 days after the cryosurgery to examine the delayed changes of the cryoinjured tissue, Result: In group A the temperatures of T1 and T2 were decreased to the $4.1{\pm}11^{\circ}C\;and\;31{\pm}5^{\circ}C$ respectively in first freezing cycle. During the second freezing period the temperatures of the thermosensors were decreased lower than the temperature during the first freezing time: $T1\;-56.4{\pm}9.7^{\circ}C,\;T2\;-18.4{\pm}14.2^{\circ}C,\;T3\;18.5{\pm}9.4^{\circ}C\;and\;T4\;35.9{\pm}2.9^{\circ}C$. Comparing the temperature-distance graph of the first cycle to that of the second cycle revealed the changes of temperature-distance relationship from curve to linear. In group B the temperatures of thermosensors were decreased and maintained throughout the 40 minutes of freezing. On light microscopy, hemorrhagic infarctions of diameter $18.6{\pm}6.4mm$ were found in group A. The infarction size was $14{\pm}3mm$ in group B. No viable cell was found within the infarction area. Conclusion: The conductivity of the lung is changed during the thawing period resulting further decrease in temperature of the lung tissue during the second freezing cycle and expanding the area of cell destruction.