• Journal of The Korean Society of Inherited Metabolic disease
• /
• v.22 no.1
• /
• pp.28-36
• /
• 2022

Purpose: Detection of abnormal metabolites in plasma amino acid (PAA) and urine organic acid (UOA) analyses has been used to diagnose clinical mitochondrial diseases, such as Leigh syndrome. In this study, the diagnostic values and effectiveness of PAA and UOA analyses were reviewed. Methods: This was a retrospective study of patients with Leigh syndrome who were diagnosed between 2003 and 2018 in a single tertiary care center. Through a whole mitochondrial sequencing and nuclear DNA associated mitochondrial gene panel analysis, 19 patients were found to be positive for mitochondrial DNA (mtDNA) mutation-associated Leigh syndrome, and 57 patients were negative. Their PAA and UOA analyses results were then compared. Results: In the comparison of the PAA and UOA analyses results between the two groups, no abnormal metabolites showed obvious differences between the mtDNA mutation-positive Leigh syndrome and mtDNA mutation-negative Leigh syndrome groups. Conclusion: PAA and UOA analyses are inappropriate test methods for diagnosing Leigh syndrome or screening of mtDNA mutation-associated Leigh syndrome. However, UOA analysis might still be a suitable screening test for Leigh syndrome.

• Journal of the Korea Institute of Building Construction
• /
• v.23 no.4
• /
• pp.465-476
• /
• 2023

This study introduces the development of a precast concrete double-wall, applicable to basement construction in apartment buildings. Unlike traditional precast concrete double walls, the developed double-wall doesn't require specialized manufacturing equipment such as a lathe. The constructability of these advanced technologies was validated through a full-scale mock-up test using the precast concrete double wall. The test specimens were constructed to represent a structural wall with a thickness of 250mm. It was observed that the quality of the in-situ concrete, filled between two single panels of 110mm thickness each, was excellent. The construction efficiency of the developed double-wall system for basement construction in an apartment building was also examined. Expert interviews about installation times of precast concrete elements were conducted to evaluate the speed of the basement floor's installation. The results showed that installation of precast concrete elements, including the proposed double walls, could be completed within 20 to 29 days for a basement in an apartment building. This indicates a three-fold increase in construction efficiency compared to traditional methods relying on in-situ casting.

• Restorative Dentistry and Endodontics
• /
• v.31 no.3
• /
• pp.153-160
• /
• 2006

We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pu)p (PF) tissues were collected from extracted intact 3rd molars. Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-$\alpha$ (TNF-$\alpha$) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P<0.05. According to this study, the results were as follows: 1. IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF) 2. IL-8 mRNA expression was not increased after stimulating 4 hrs with SP ($10^{-5}M$) and SP ($10^{-8}M$) compared with Mock stimulation in all type of cells studied. 3. IL-8 mRNA expression was not increased after stimulating 8 hrs with SP ($10^{-4}M$) and CGRP ($10^{-6}M$) compared with Mock stimulation in all type or cells studied. 4. TNF-$\alpha$ (2 ng/ml) increased the expression of IL-8 mRNA in all kind of cells studied. 5. The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP ($10^{-6}M$)(p<0.05). 6. The secretion of IL-8 from PDLF was. increased 8 hrs after the stimulation with SP ($10^{-4}M$)(p<0.05). Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue , whereas Substance P increased the secretion of IL-8 from periodontal ligament.

• Journal of the Korea Concrete Institute
• /
• v.19 no.6
• /
• pp.755-762
• /
• 2007

For the evaluation of the load carrying capacity of the deteriorated PSC I girder bridge in service load state and the verification of the grade to the reinforcement effect of actual bridge strengthened by external prestressing tendons, the field test using vehicles is applied widely. Because this teat was executed in elastic range, the investigation of the characteristics of behavior caused by live load is only available. And it is impossible to estimate load carrying capacity in limit state and nonlinear behavior after that a crack is appeared. In this study, the 27-year-old prestressed concrete girder bridge is used and various load tests are performed, so we evaluate the behavior characteristics of the bridge in service load state and ultimate load state, and estimate the load carrying capacity of bridge. In addition, the artificial damages are induced from cutting internal tendons, and external tendons is added to strengthen it as much as vanished internal tendons. Next we compare the damage state with the strengthening state. In case of the application of external prestressing method to PSC I girder bridge, the present experiment result may decide more exactly the load carrying capacity of actual bridge, the amount for reinforcement, and the standard of quality control etc. at reinforcement work.

• The Journal of Engineering Geology
• /
• v.26 no.1
• /
• pp.79-85
• /
• 2016

A concrete silo plays an important role in subsurface low- and intermediate-level waste facilities (LILW) by limiting the release of radionuclides from the silo geosphere. However, due to several physical and chemical processes the performance of the concrete structure decreases over time and consequently the concrete loses its effectiveness as a barrier against groundwater inflow and the release of radionuclides. Although a number of processes are responsible for degradation of the silo concrete, it is determined that the main cause is corrosion of the reinforcing steel. Therefore, the time it takes for the silo concrete to fail is calculated based on two factors: the initiation time of corrosion, defined as the time it takes for chloride ions to penetrate through the concrete cover, and the propagation time of corrosion. This paper aims to estimate the time taken for concrete to fail in a LILW disposal facility. Based on the United States Department of Energy (DOE) approach, which indicates that concrete fails completely once 50% of the volume of the reinforcing steel corrodes, the corrosion propagation time is calculated to be 640 years, which is the time it takes for corrosion to penetrate 0.640 cm into the reinforcing steel. In addition to the corrosion propagation time, a diffusion equation is used to calculate the initiation time of corrosion, yielding a time of 1284 years, which post-dates the closure time of the LILW disposal facility if we also consider the 640 years of corrosion propagation. The electrochemical conditions of the passive rebar surface were modified using an acceleration method. This is a useful approach because it can reduce the test time significantly by accelerating the transport of chlorides. Using instrumental analysis, the physicochemical properties of corrosion products were determined, thereby confirming that corrosion occurred, although we did not observe significant cracks in, or expansion of, the concrete. These results are consistent with those of Smartet al., 2006 who reported that corrosion products are easily compressed, meaning that cracks cannot be discerned by eye. Therefore, it is worth noting that rebar corrosion does not strongly influence the hydraulic conductivity of the concrete.

• Journal of the Korea Concrete Institute
• /
• v.28 no.4
• /
• pp.473-480
• /
• 2016

The purpose of this study is to provide analytical method to reasonably evaluate the complicated failure behaviors of shear friction of reinforced concrete shear wall specimens using grade 500 MPa high-strength bars. A total of 16 test specimens with a variety of variables such as aspect ratio, friction coefficient of interface in construction joint, reinforcement details, reinforcement ratio in each direction, material properties were selected and the analysis was performed by using a non-linear finite element analysis program (RCAHEST) applying the modified shear friction constitutive equation in interface based on the concrete design code (KCI, 2012) and CEB-FIP Model code 2010. The mean and coefficient of variation for maximum load from the experiment and analysis results was predicted 1.04 and 17% respectively and properly evaluated failure mode and overall behavior characteristic until failure occur. Based on the results, the analysis program that was applied modified shear friction constitutive equation is judged as having a relatively high reliability for the analysis results.

• Korean Journal of Construction Engineering and Management
• /
• v.11 no.3
• /
• pp.13-22
• /
• 2010

From now on, the aged apartment or house is expected to increase rapidly. So, we have to build a process of remodeling and develop the new technique. Demolition work is needed for systematic plan and management. However, contractors of the remodeling project established a rough plan and did not consider recycling wastes, safety of workers and structural stability of building. Therefore, we need a step to develop a assessment system, verify and make specified. This paper evaluated how much improve on construction speed, work efficiency, intensity of work and influence with another process comparing the existing method with the new demolition method. The qualitative and quantitative assessment system are developed with these output. The case study was carried out experimental group and control group, based on developed assessment system, which have the same condition. The existing method was made up of 3 steps- 1)Demolish windows, doors and iron goods, 2)Demolish indoor and outdoor walls, 3)Drop the waste. The new demolition method was made up of 5 steps- 1)Demolish windows, doors and iron goods, 2)Demolish the ceiling and wall's finishing materials, 3)Demolish the floor's finishing materials, 4)Demolish indoor and outdoor walls, 5)Drop the waste. Work time, idle time, the character of a work unit are analyzed by mock-up test. This study's output is expected to establish a systematic process of new demolition method and based on the maximizing waste recycling work in our construction industry.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.9
• /
• pp.130-138
• /
• 2018

This study was a descriptive study, analyzing the clinical judgment process that occurs in a simulation of practice education for nursing students applying to LCJR. Subjects were two nursing college students in S city. Fever scenario and apnea scenario, including mock patient, were implemented. Data collection was conducted between September, 2013 and December, 2014. Data analysis was performed using descriptive statistics, paired t-test, and Pearson's correlation of PASW statistics 18.0 program. The result of this study revealed that the clinical judgment scores of nursing college students were 30.50 for males and 29.32 for females. Nursing Clinical judgment score for the apnea scenario was higher than the score for the febrile scenario, and nursing students' self-evaluation and professors' evaluation showed a significant correlation with respect to the responding domain. In comparison the student and faculty ratings, domain of interpreting and domain of reflecting were significant This study will provide educators with foundational knowledge of program development to enhance nursing students' clinical judgment abilities. We suggest more discussion on their nursing practice and judgment during debriefing session may be beneficial for students.

• Journal of Korean Society of Steel Construction
• /
• v.26 no.4
• /
• pp.361-373
• /
• 2014

As the height and beam span of buildings built in the construction market increase, increasingly higher quality is being required of the construction materials. In response to this trend, 800MPa tensile strength class steel was developed in domestic company. Currently, experiments applying flexural member, compression member, and connections are continuously conducted, but a design guideline for high strength steel has yet to be established. Among those construction materials, for the high strength steel beam-to-column connections, the evaluation of implementing ductile connections for the high strength steel beam-to-column connections is producing pessimistic results and the number of related researches is inadequate because of the high yield ratio, which is the characteristic of high strength steel. This study on implementation of ductile connections made of high strength steel was conducted using the connection detail as the variable, for the purpose of enhancing the deformation capacity of high strength steel beam-to-column connections. Cyclic loading test and nonlinear finite element analysis were conducted with full-scale mock-up connection models with the applied connection details. As a result, the structural performance of high-strength steel beam-to-column connection with presented detail was contented with demand of Special Moment Frames of KBC standard.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2008.04a
• /
• pp.789-792
• /
• 2008

Blast furnace slag doesn't have self-hydraulicity and it needs stimulants such as alkali to hydrate. Therefore using recycled aggregates erupted calcium hydroxides and blast furnace slag acquiring alkali stimulate could make a complementarily use of a recycling architectural material possible. In this study, we have discussed about characters of blast furnace slag and recycled aggregate firstly, and make recycled aggregate mortar and concrete using blast furnace slag for the experiment. The experiment is about mortar and concrete using recycled aggregate as a substitutional material of blast furnace slag. In this experiment, I replace blast furnace slag and aggregate with recycled aggregate. Conclusions through the test results analysis are as follows. And then, we added field experiment using concrete with composited materials.