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Treatments of the Compartment Syndrome of the Foot after the Calcaneal Fractures (종골 골절후 발생한 족부 구획 증후군의 치료)

  • Park, Yong-Wook;Chung, Yung-Khee;Yoo, Jung-Han;Jun, Deuk-Soo;Hwang, Pil-Sung
    • Journal of Korean Foot and Ankle Society
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    • v.4 no.2
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    • pp.93-99
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    • 2000
  • Eleven patients with calcaneal fracture had 2 acute compartment syndrome of the feet and 9 late complication of the compartment syndrome of the feet. An interstitial pressure of more than 30 mmHg in either the central or interosseous compartment was considered pathologic and was treated by fasciotomy performed medially. Rigid claw toe deformity was treated by excision of the head and neck of the proximal phalanx, dorsal capsular release of the metatarsophalangeal joint, and lengthening of the extensor tendon. Patients were evaluated at a mean of 35 months(range, 21 - 44 months) after operation, and the examination was directed specifically toward symptoms and signs of myoneural ischemia, and walking pain. At follow-up, 2 patients with acute compartment syndrome of the foot had no evidence of myoneural ischemia, 9 patients with claw toe deformity had no pain with walking. Based on our clinical observations, we concluded that compartment syndrome of the foot may occur after the calcaneal fracture and fasciotomy is effective treatment for the prevention of the long term sequelae of this debilitating condition.

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Regeneration of Plants from EMS-treated Immature Embryo Cultures in Soybean [Glycine max(L.) Merr.]

  • Van, Kyu-Jung;Jang, Hyun-Ju;Jang, Young-Eun;Lee, Suk-Ha
    • Journal of Crop Science and Biotechnology
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    • v.11 no.2
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    • pp.119-126
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    • 2008
  • Since somatic embryogenesis combined with ethylmethane sulfonate(EMS) treatments is the most efficient technique for mutagenesis, the embryogenic capacity of four soybean cultivars was evaluated at different EMS concentrations, treatment times, and preculture durations. Two to 4 mm long immature cotyledons were placed in induction medium after EMS treatment, and the numbers of somatic embryos formed per explant were counted four weeks after culture initiation. We observed genotypic differences in the efficiency of somatic embryogenesis from immature embryos among four cultivars treated with different concentrations of EMS for six hours. Cultivars, Sinpaldalkong 2 and Jack, displayed highly efficient somatic embryogenesis regardless of EMS concentration, whereas very low efficiency or no survival was observed in Jinju 1 and Iksannamulkong cultivars. Preculture duration did not influence the efficiency of somatic embryogenesis. Because Sinpaldalkong 2 exhibited the best somatic embryogenesis, much higher concentrations of EMS were used to test somatic embryo formation under different periods of time in this cultivar. Three and six hour treatments with both 1 and 2 mM EMS yielded higher embryo formation than longer periods of time. Increasing the time with embryos in 2 mM EMS caused a reduction in somatic embryogenesis in Sinpaldalkong 2, but many chlorophyll-deficient soybean variants were identified in the $M_1R_0$ and $M_2R_1$ generations. In addition to Jack, Sinpaldalkong 2 is a good genotype for plant regeneration from EMS-treated immature embryo cultures.

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Characterization of a Vibrio parahaemolyticus Phage Isolated from Marine (해양에서 분리한 Vibrio parahaemolyticus Phage의 특성)

  • Yoon, Sun-Ok;Ju, Seong-A;Heo, Moon-Soo;Jung, Cho-Rok;Ju, Jin-Woo
    • The Journal of the Korean Society for Microbiology
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    • v.34 no.5
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    • pp.423-433
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    • 1999
  • A novel bacteriophage, designated as VPP97, that infects the strains of Vibiro parahaemolyticus (hallophilic, Gram-negative bacterium) isolated most commonly from marine environments, has been discovered, and several of its properties have been determined. The plaques were clear and sized $0.6{\sim}1.0\;mm$ in diameter. The virion forms a single band on 70% sucrose gradient and ${\rho}1.50$ CsCl gradient by sucrose gradient centrifugation and CsCl gradient centrifugation respectively. It has a hexagonal head and a relatively long tail, as shown by electron microscopy. Vibrio alginolyticus, Vibrio fluvialis and Vibrio furnissii were also sensitive to this phage. It was almost totally inactivated at $70^{\circ}C$ and at pH below 5 or over 10. The nucleic acid of VPP97 is composed of DNA. The VPP97 had 9 specific structural proteins sized between 21.5 kDa and 97.4 kDa on SDS-PAGE. When V. parahaemolyticus cultures were treated with either phage VPP97 or one of the several antibiotics for 2 hours, the viable number of V. parahaemolyticus treated with the phage VPP97 is lower than that treated with chloramphenicol, erythromycin or penicillin, but not lower than that treated with tetracycline. Mice that have responded to the phage treatment revealed the lower numbers of V. parahaemolyticus in small intestine and less damage on small intestine compared to the untreated mice. Therefore, we suggest that the phage treatment appears effective to the infection by V. parahaemolyticus.

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Study on Baled Silage Making of Selected Forage Crop and Pesture Grasses I. Discussion on baled silage making as affected by phtsiological characteristics of tth plants (주요 사료작물의 곤포 Silage 조제이용에 관한 연구 I. 작물의 생리적 특성과 곤포 Silage 조제이용)

  • 김정갑;강우성;한정대;신정남;한민수;김건엽
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.15 no.1
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    • pp.73-79
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    • 1995
  • A simple conservation technique baled silage making of selected froage materials was discussed in Suwon and in Muan county during 1991 - 1992. Eleven species of forage crops and pasture grasses(maize. sorghum, pearl millet, barnyardgrass, rye, barley, spring oat, Italian ryegrass, orchardgrass, alfalfa and grass-legume pasture mixtures) were harvested at different growth stage from young plant to maturity and baled in a self constructed square baling chamber, and wrapped in a 0.05mm thick polyethylene plastic film. Each bales measured by 90cm long, 60cm wide and 50cm height and weighted between 15~20kg in dry matter basis. physio-molphologcal characteristics of the plants, leaf weight ratio(LWR), leaf area ratio(LAR), stalk ratio (SR), stalk hardness(SH) and other growth parameters, were analysed and were used as a parameter to evaluate the suitability of materials for baling. Italian ryegrass including orchardgrass, alfalfa and pasture mixtures produced high quality baled silage. Silage quality point(F1ieg-point) of Italian ryegrass was improved from 63 point in crushed custom silage to 75 piont in baled silage. Meterial of grass-legume pasture mixtures showed 55 point in crushed silage and 67 point in baled silage. Fodder rye, barley, spring oat and barnyardgrass were also evaluated as a good materials for baled silage making. On the other hand, meize including sorghum and peral millet were evaluated as a not suitable materals for baling due to its high value of SR and SH. Quality of maize was excellent with 88 point in clushed silage and medium with 47 point in baled silage making.

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Quantitative Analysis of the Fifteen Constituents in Hyangso-San by LC-MS/MS (LC-MS/MS를 이용한 향소산 중 15종 성분의 정량분석)

  • Seo, Chang-Seob;Shin, Hyeun-Kyoo
    • Korean Journal of Pharmacognosy
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    • v.47 no.4
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    • pp.381-388
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    • 2016
  • Hyangso-san is a traditional herbal medicine that consists of the seven herbal medicines, Cyperi Rhizoma, Perillae Folium, Atractylodis Rhizoma, Citri Unshius Pericarpium, Glycyrrhizae Radix et Rhizoma, Zingiberis Rhizoma Crudus, and Allii Fistulosi Bulbus. Hyangso-san has long been clinically used to treat the influenza, including headache, ferver, chills, and pantalgia. In this study, we were performed the simultaneous analysis of the 15 marker compounds (liquiritin apioside, liquiritin, ferulic acid, naringin, hesperidin, rosmarinic acid, liquiritigenin, kaempferol, glycyrrhizin, nobiletin, 6-gingerol, elemicin, atractylenolide III, nootkatone, and atractylenolide I) in Hyangso-san using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS). Column for the separation of the 15 ingredients was used a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}m$) at $45^{\circ}C$ by using a mobile phase of 0.1% (v/v) formic acid in water and acetonitrile with gradient condition. Identifications of all analytes were performed using a Waters ACQUITY TQD LC-MS/MS system. The flow rate and injection volume were 0.3 mL/min and $2.0{\mu}L$, respectively. Correlation coefficient of the calibration curve was ${\geq}0.9958$. The values of limits of detection and quantification of the 15 components were 0.002-4.29 and 0.01-12.88 ng/mL, respectively. The result of an analysis using the established LC-MS/MS method, kaempferol and atractylenolide I were not detected, while other 13 compounds were 0.08-56.87 mg/g in lyophilized Hyangso-san sample.

Wavelength selection by loading vector analysis in determining total protein in human serum using near-infrared spectroscopy and Partial Least Squares Regression

  • Kim, Yoen-Joo;Yoon, Gil-Won
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.4102-4102
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    • 2001
  • In multivariate analysis, absorbance spectrum is measured over a band of wavelengths. One does not often pay attention to the size of this wavelength band. However, it is desirable that spectrum is measured at only necessary wavelengths as long as the acceptable accuracy of prediction can be met. In this paper, the method of selecting an optimal band of wavelengths based on the loading vector analysis was proposed and applied for determining total protein in human serum using near-infrared transmission spectroscopy and PLSR. Loading vectors in the full spectrum PLSR were used as reference in selecting wavelengths, but only the first loading vector was used since it explains the spectrum best. Absorbance spectra of sera from 97 outpatients were measured at 1530∼1850 nm with an interval of 2 nm. Total protein concentrations of sera were ranged from 5.1 to 7.7 g/㎗. Spectra were measured by Cary 5E spectrophotometer (Varian, Australia). Serum in the 5 mm-pathlength cuvette was put in the sample beam and air in the reference beam. Full spectrum PLSR was applied to determine total protein from sera. Next, the wavelength region of 1672∼1754 nm was selected based on the first loading vector analysis. Standard Error of Cross Validation (SECV) of full spectrum (1530∼l850 nm) PLSR and selected wavelength PLSR (1672∼1754 nm) was respectively 0.28 and 0.27 g/㎗. The prediction accuracy between the two bands was equal. Wavelength selection based on loading vector in PLSR seemed to be simple and robust in comparison to other methods based on correlation plot, regression vector and genetic algorithm. As a reference of wavelength selection for PLSR, the loading vector has the advantage over the correlation plot since the former is based on multivariate model whereas the latter, on univariate model. Wavelength selection by the first loading vector analysis requires shorter computation time than that by genetic algorithm and needs not smoothing.

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Rosenvingea orientalis (Scytosiphonaceae, Phaeophyceae) from Chiapas, Mexico: life history in culture and molecular phylogeny

  • West, John A.;Zuccarello, Giuseppe C.;Pedroche, Francisco F.;De Goer, Susan Loiseaux
    • ALGAE
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    • v.25 no.4
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    • pp.187-195
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    • 2010
  • The genus Rosenvingea is well known in the tropics. Four species have been reported from Pacific Mexico: R. floridana, R. antillarum, R. intricata and R. sanctae-crucis. We collected a plant (Boca del Cielo, Chiapas) that we identified as Rosenvingea orientalis, a species not previously reported from Pacific Mexico. We were able to characterize the life cycle of this species for the first time in laboratory culture. It reproduced exclusively by plurilocular sporangia (plurangia). The mature plants were up to 6 cm long with cylindrical to compressed fronds (to 2 mm wide) with dichotomous branches in the upper half of the thallus. The medulla was hollow with 2-3 layers of large inflated colourless cells at the periphery. The cortex was comprised of 1 layer of small cells, each with a single chloroplast and pyrenoid. Linear plurangial sori with phaeophycean hairs formed along the mature fronds. Zoospore germlings developed into prostrate filamentous systems, each with a single phaeophycean hair that gave rise to a single erect shoot with multiple hairs arising near the tip. Molecular phylogeny using the psaA gene placed this isolate within the Scytosiphonaceae. It does not confirm the exact identification of R. orientalis, although its placement close to other Rosenvingea sequences was confirmed and morphological evidence supports its placement in R. orientalis. Our culture investigations indicated that it has an asexual life cycle. Further collections are needed to resolve the full generic and specific relationships of Rosenvingea and related taxa, and their reproductive patterns.

Historical Review on the Parabolic Reflector Moxa and Clinical Applications of Light Moxibustion in Korean Medicine (양수구(陽燧灸)(오목거울뜸)에 대한 역사적 고찰 및 한의임상(韓醫臨床)에서 광선구(光線灸)의 활용 전망)

  • Jang, In-Soo;Sun, Seung-Ho;Seo, Hyung-Sik
    • Korean Journal of Acupuncture
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    • v.27 no.4
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    • pp.1-4
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    • 2010
  • Objectives : The purpose of this study is to summarize the historical aspects of parabolic reflector moxa and to suggest the applications of light moxibustion in Korean medicine. Results : Getting a fire by parabolic reflector(concave mirror) is well known because of the Olympic torch lighted with a solar reflector in Greece. From anecdotes or myths for Diocles and Archimedes(third century B.C.) a long history has been chronicled in books and films in western culture. However, it has another history in Asian countries since 2,000 years ago. Some reflectors had a tiny size with 6.5 to 10 cm in diameter, 4 mm to 1 cm thickness, and these mobile handheld devices could make us guess that they have been used for multiple purposes. Bronze reflectors were described in Dongeui-bogam, and excavated from the ancient remains of the Shilla and Koryo dynasties. This common firing device was used as a moxibustion device, one of the acupuncture and moxibustion modalities in traditional Korean medicine. Conclusions : Reflector moxa has been used as a light moxibustion to deliver heat energy to acupuncture points, muscles, and skin along meridians. We present a plausible proposal to improve other phototherapy modalities including reflector moxa in Korean medicine practice.

TREATMENT OF CONDYLE FRACTURE WITH RESORBABLE SCREW (흡수성 스크류를 이용한 하악과두 골절의 치료)

  • Yeo, In-Bum;Min, Seung-Ki;Oh, Sung-Hwan;Kwan, Kyung-Hwan;Choi, Sang-Moon;Park, Sang-Kyu
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.28 no.6
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    • pp.559-564
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    • 2006
  • According to the development of operation technique and biologic materials, oromaxillofacial surgery department have used many kinds of metal and biologic materials in ORIF and plastic surgery. In maxillofacial fracture, ORIF with metal plate and screw have short healing period and good prognosis. But ORIF with metal materials have many complications as maxillofacial abnormal growth, screw loosening, bone malunion. And metal materials have not used in infection site. The purpose of this study is to evaluate the clinical value of 10 condylar fracture patients operated with absorbable screw at Wonkwang university. Ten patients(8 males, 2 female, mean aged 28) who had mandibular condyle process fracture treated with PLLA implants(poly-l-lactide) was recalled for follow-up clinical and radiologic examination for 10 years. Mouth opening recorvered to more than 35mm and occlusion was stable in all patients. All fractured mandibular condyles showed anatomic good reduction and long-term stability with the use of resorbable miniplates and screw. Bone healing was satisfactory in all patients, and there was no evidence of abnormal resorption of condylar process.

Piezoelectric Vibration Energy Harvester Using Indirect Impact (간접 충격을 이용한 압전 방식 진동형 에너지 하베스터)

  • Ju, Suna;Ji, Chang-Hyeon
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.66 no.10
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    • pp.1499-1507
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    • 2017
  • This paper presents an impact-based piezoelectric vibration energy harvester using a freely movable metal sphere and a piezoceramic fiber-based MFC (Macro Fiber Composite) as piezoelectric cantilever. The free motion of the metal sphere, which impacts both ends of the cavity in an aluminum housing, generates power across a cantilever-type MFC beam in response to low frequency vibration such as human-body-induced motion. Impacting force of the spherical proof mass is transformed into the vibration of the piezoelectric cantilever indirectly via the aluminum housing. A proof-of-concept energy harvesting device has been fabricated and tested. Effect of the indirect impact-based system has been tested and compared with the direct impact-based counterpart. Maximum peak-to-peak open circuit voltage of 39.8V and average power of $598.9{\mu}W$ have been obtained at 3g acceleration at 18Hz. Long-term reliability of the fabricated device has been verified by cyclic testing. For the improvement of output performance and reliability, various devices have been tested and compared. Using device fabricated with anodized aluminum housing, maximum peak-to-peak open-circuit voltage of 34.4V and average power of $372.8{\mu}W$ have been obtained at 3g excitation at 20Hz. In terms of reliability, housing with 0.5mm-thick steel plate and anodized aluminum gave improved results with reduced power reduction during initial phase of the cyclic testing.