The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Characterization of a Vibrio parahaemolyticus Phage Isolated from Marine

해양에서 분리한 Vibrio parahaemolyticus Phage의 특성

  • Yoon, Sun-Ok (Department of Microbiology, Graduate School, Pusan National University) ;
  • Ju, Seong-A (Division of Biological Sciences, College of Natural Science, Ulsan University) ;
  • Heo, Moon-Soo (Faculty of Applied Marine Sciences, College of Ocean Sciences, Cheju National University) ;
  • Jung, Cho-Rok (Department of Microbiology, Graduate School, Pusan National University) ;
  • Ju, Jin-Woo (Department of Microbiology, College of Natural Science, Pusan National University)
  • 윤선옥 (부산대학교 자연과학대학 대학원 미생물학과) ;
  • 주성아 (울산대학교 자연과학대학 생명과학부) ;
  • 허문수 (제주대학교 해양과학대학 해양생산과학부) ;
  • 정초록 (부산대학교 자연과학대학 대학원 미생물학과) ;
  • 주진우 (부산대학교 자연과학대학 미생물학과)
  • Published : 1999.10.30
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Abstract

A novel bacteriophage, designated as VPP97, that infects the strains of Vibiro parahaemolyticus (hallophilic, Gram-negative bacterium) isolated most commonly from marine environments, has been discovered, and several of its properties have been determined. The plaques were clear and sized $0.6{\sim}1.0\;mm$ in diameter. The virion forms a single band on 70% sucrose gradient and ${\rho}1.50$ CsCl gradient by sucrose gradient centrifugation and CsCl gradient centrifugation respectively. It has a hexagonal head and a relatively long tail, as shown by electron microscopy. Vibrio alginolyticus, Vibrio fluvialis and Vibrio furnissii were also sensitive to this phage. It was almost totally inactivated at $70^{\circ}C$ and at pH below 5 or over 10. The nucleic acid of VPP97 is composed of DNA. The VPP97 had 9 specific structural proteins sized between 21.5 kDa and 97.4 kDa on SDS-PAGE. When V. parahaemolyticus cultures were treated with either phage VPP97 or one of the several antibiotics for 2 hours, the viable number of V. parahaemolyticus treated with the phage VPP97 is lower than that treated with chloramphenicol, erythromycin or penicillin, but not lower than that treated with tetracycline. Mice that have responded to the phage treatment revealed the lower numbers of V. parahaemolyticus in small intestine and less damage on small intestine compared to the untreated mice. Therefore, we suggest that the phage treatment appears effective to the infection by V. parahaemolyticus.

Keywords

References

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