• 제목/요약/키워드: mixed-sugar medium

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Production of DagA, a ${\beta}$-Agarase, by Streptomyces lividans in Glucose Medium or Mixed-Sugar Medium Simulating Microalgae Hydrolysate

  • Park, Juyi;Hong, Soon-Kwang;Chang, Yong Keun
    • Journal of Microbiology and Biotechnology
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    • 제24권12호
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    • pp.1622-1628
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    • 2014
  • DagA, a ${\beta}$-agarase, was produced by cultivating a recombinant Streptomyces lividans in a glucose medium or a mixed-sugar medium simulating microalgae hydrolysate. The optimum composition of the glucose medium was identified as 25 g/l glucose, 10 g/l yeast extract, and $5g/l\;MgCl_2{\cdot}6H_2O$. With this, a DagA activity of 7.26 U/ml could be obtained. When a mixed-sugar medium containing 25 g/l of sugars was used, a DagA activity of 4.81 U/ml was obtained with very low substrate utilization efficiency owing to the catabolic repression of glucose against the other sugars. When glucose and galactose were removed from the medium, an unexpectedly high DagA activity of about 8.7 U/ml was obtained, even though a smaller amount of sugars was used. It is recommended for better substrate utilization and process economics that glucose and galactose be eliminated from the medium, by being consumed by some other useful applications, before the production of DagA.

Carrageenan 필름의 미생물에 의한 생분해도 측정 (The Bildegradability of Carrageenan-based Film by Microorganisms)

  • 강성국;정순택;박현진;임종환
    • 한국미생물·생명공학회지
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    • 제23권6호
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    • pp.702-709
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    • 1995
  • Degradation Of $\kappa $-carrageenan-based film by microorganisms screened from carrageenan source and activated sludge of a carrageenan producing factory was investigated by measuring changes of pH, viscosity, total sugar and total organic carbon (TOC) of the medium containing $\kappa $-carrageenan as a carbon source. Initially fifteen strains of microorganism were isolated from carrageenan source and activated sludge and then three organisms among them were selected based on the ability of growing in the medium including 0.3% $\kappa $-carrageenan as a sole carbon source. They were identified as Escherichia coli, Saccharomyces cerevisiae and Aspergillus niger. As indices of biodegradability Of $\kappa $-carrageenan based film, the changes of pH, viscosity, total sugar, and TOC of the carrageenan film-based medium were tested by the cultivation of single or mixed strains of the identified organisms. Mixed culture showed the highest biodegradability, which resulted in the changes of pH from 6.5 to 3.0, viscosity from 164 cps to 15.6 cps, total sugar content from 2.35 g/l to 0.53 g/l and TOC from 5721 ppm to 232 ppm after 30 days of cultivation. The biodegradability determined as the reduction rate of TOC by pure cultures of Asp. niger, E. coli, Sacch. cerevisiae and mixed culture of the three organisms were 94%, 86%, 80% and 96%, respectively.

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심비디움 Pine Clash 'Moon Venus'의 생장 및 양분함량에 미치는 폐타이어칩의 영향 (Effect of Waste Tire Chips on the Growth and Nutrient Content of Cymbidium Pine Clash 'Moon Venus')

  • 김홍열
    • 농업생명과학연구
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    • 제43권2호
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    • pp.17-21
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    • 2009
  • 본 실험은 폐타이어칩이 심비디움 Pine Clash 'Moon Venus'의 생장 및 양분함량에 미치는 영향에 대해서 조사하였다. 잎, 줄기의 지상부 생장은폐타이어칩과 바크를 혼용한 경우에는 관행구인 바크와 차이가 없었다. 그러나 폐타이칩 단용구에서는 지상부의 생장이 현저하게 감소하였다. 총근수, 신근수, 근장 등 지하부의 생장도 지상부의 생장과 유사한 경향을 나타내었다. 부패근수는 폐타이어칩 중립과 대립의 단용구에서 증가하였다. 전당, 전분, 엽록소와 N, P, K 등 무기양분의 함량도 폐타이어칩과 바크를 혼용한 경우에는 관행구인 바크와 차이가 없었으나, 폐타이칩 단용구에서는 감소하였다.

호알칼리성 목질분해 효소를 이용한 폐지 재생(제2보) - 알칼리성 목질분해 효소 정제 및 섬유 반응 특성 - (Recycling of Waste Paper with Alkaline Cellulolytic Enzyme (II) - Purification of alkaline cellulolytic enzymes and characteristics of reaction with fiber -)

  • 강석현;이중명;박성배;엄태진
    • 펄프종이기술
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    • 제36권1호
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    • pp.24-29
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    • 2004
  • Alkaline cellulolytic enzymes from cultured medium of Coprinus cinereus 2249 were purified with gel and ion-exchange chromatography and characteristics of those enzyme proteins were investigated. A fiber length distribution and a crystallinity of cellulose and sugar composition of enzyme treated Mixed Office Wastepaper(MOW) and Unbleached Kraft Pulp(UKP) were analysed. The conclusion could summarized as follows; \circled1 Alkaline and acidic, endo- and exo-glucanases were purified from cultured medium of Coprinus cinereus 2249. \circled2 The approximate molecular weight of alkaline endo-glucanase was 42 kDa, and also that of alkaline exo-glucanase was 50 kDa. A fiber length distribution and a crystallization of cellulose and sugar composition of enzyme treated MOW and UKP were not so much changed with original paper and pulp.

당발효에 미치는 Lactobacillus helveticus YM-1 과 Streptococcus lactis ${ML}_3$의 상호작용 (Interaction of Lactobacillus helveticus YM-1 and Streptococcus lactis ${ML}_3$ on the Sugar Fermentation in Skim Milk)

  • 박정길;류인덕;윤성식;유주현
    • 한국식품과학회지
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    • 제19권1호
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    • pp.42-49
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    • 1987
  • L.helveticus YM-1 과 Str.lactis ${ML}_3$를 탈지유, 배양여액및 함성배지에 단독및 혼합배양 하였을때 산생성량과 당이용성 그리고 상관관계를 검토하였다. 자기자신의 배양여액보다 상대편의 배양여액에서, 단독배양보다 혼합배양의 경우가 산생성 촉진효과가 컸었다. 유당의 이용성은 12시간 배양했을때 Str. lactis ${ML}_3$ 의 경우 0.4%, L.helveticus YM-1은 0.85%. 혼합배양시는 1.05%였다. L.helveticus YM -1의 단독배양과 두균의 혼합배양의 경우 8시간후 배지에 생성된 포도당은 0.22%와 0.10%를 나타낸 다음 점차 감소하였다. L.helveticus YM-1에 의해서 생성된 포도당은 Str.lactis ${ML}_3$의 산생성을 촉진한다고 생각된다.

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Propionibacterium freudenreichii KFCC 31227과 Lactobacillus acidophilus KFCC 32825의 단독 및 혼합배양에 의한 영양성분의 상호작용 (Identification of the growth stimulatory components in the single and mixed cultures of Propionibacterium freudenreichii KFCC 31227 and Lactobaciilus acidophilus KFCC 32825)

  • 민윤식;김기철;이명열;이응수
    • Applied Biological Chemistry
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    • 제36권5호
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    • pp.352-357
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    • 1993
  • S.G.Y.배지(skim milk, glucose, yeast extract)와 S.M.W.배지(skim milk whey). 배지에 P. freudenreichii KFCC 31227과 L. acidophilus KFCC 32825를 단독 또는 혼합배양 후, 두 균주의 생육 및 유기산 생성에 미치는 발효여액의 영향과 당의 변화 및 이용 효과를 검토한 결과, 자신의 발효여액보다는 상대편의 발효여액에서 그리고 단독배양보다는 혼합배양에서 생육이나 산 생성의 효과가 촉진되었으며, 배지중의 glucose 및 lactose 의 이용효과를 보면 P. freudenreich-ii KFCC 31227 단독 배양시는 lactose 를, L. acdophilus KFCC 32825 단독배양시는 glucose를 생육이나 산 생성에 더 잘 이용하였다. 또한 두 균주의 혼합배양시에는 단독배양에 비해 발효에 glucose 나 lactose 를 이용하는 시간이 더욱 단축되는 결과로 나타났으며, 이것은 혼합배양시 두 균주의 상호작용에 의한 synergism 효과에 기인된 것으로 생각된다.

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미세조류 Microcystis aeruginosa로부터 바이오 알콜의 생산 (Bioalcohol Production with Microalgae, Microcystis aeruginosa)

  • 김종덕;채고은;서효진;차우드리 나렌드라;윤양호;신태선;김민용
    • KSBB Journal
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    • 제27권6호
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    • pp.335-340
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    • 2012
  • The microalgae, Microcystis aeruginosa are able to proliferate in a wide range of freshwater ecosystem. M. aeruginosa was cultivated in 25 L and 240 L race-way reactor containing modified medium with added urea 0.2 g/L, increased $Fe^{+2}$, and decreased $Ca^{+2}$ion compared to BG11 medium. Sugar contents of M. aeruginosa grown in BG11 medium, and modified medium were 120 mg/mL and 140 mg/mL respectively. Fermentation was conducted with the extract of M. aeruginosa at $30^{\circ}C$ for 30 h, using Saccharomyces cerevisiae (Sc), Pichia stipitis (Ps), Zymomonas mobilis (Zm), and mixed-culture of these strains (Sc + Ps + Zm). Pichia stipitis (0.7%) was found to be more suitable for producing bioalcohol from M. aeruginosa extract than other strains of Saccharomyces cerevisiae (0.45%) and Zymomonas mobilis (0.61%), while mixed-cultured of these strains showed higest productivity by 1.75%. Biomass of M. aeruginosa contains the potency to be the most renewable resource for bioalcohol fermentation.

미생물(微生物) Tannase를 이용한 도토리주(酒)의 실험적(實驗的) 제조(製造) (Experimental Manufacture of Acorn Wine by Fungal Tannase)

  • 채수규;유태종
    • 한국식품과학회지
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    • 제15권4호
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    • pp.326-332
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    • 1983
  • 미생물 tannase를 식품가공에 응용하기 위한 하나의 연구로 도토리 tannin 분해력이 강한 Aspergillus sp. AN-11 균주를 접종하여 만든 곡자를 이용하여 도토리주(酒)의 실험적 제조를 시도하였다. 도토리의 전분가(澱粉價)는 72.84 로서 탄수화물(炭水化物) 식품(食品)으로서 개발(開發) 이용(利用) 가치가 있었다. 도토리 주조용(酒造用)으로 적합한 곡자로서 발효에 저해작용을 나타내는 tannin 성분을 효과적으로 분해하기 위하여 쌀과 도토리를 50 : 50 으로 혼합하고 여기에 당화력(糖化力)이 강한 Aspergillus oryzae 균주와 tannase 활성도(活性度)가 우수한 Aspergillus sp. AN-11 균주를 접종하여 제조한 혼합 절충곡자를 선정하여 사용하였다. 도토리분(粉)을 전처리로서 하룻밤 침수시켜 여분의 물을 제거한 후 증자한 것을 담금 시료로 하여 선정된 혼합 절충곡자들 사용하여 발효시킨 시험구(試驗區)가 전체 발효과정을 통하여 알코올 생성 속도 및 당의 발효율이 무처리한 도토리분(粉)에 보통 곡자를 사용한 시험구에 비하여 약 2배정도 우세한 경향을 나타내었다.

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밀가루 및 당 농도가 소다크랙커의 스폰지 발효에 미치는 영향 (Effect of Concentrations of Wheat Flour and Sugar on Sponge Fermentation of Soda Cracker)

  • 김상용;이병돈;김정민;오덕근
    • 한국식품과학회지
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    • 제29권2호
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    • pp.387-389
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    • 1997
  • L. brevis, L. fermentum과 L. plantarum의 혼합젖산균을 사용하여 소다크랙커의 스폰지 발효과정 중에 밀가루와 당 농도가 미치는 양향을 살펴보았다. 밀가루 전분이 혼합젖산균에 의하여 포도당으로 전환되어 이용되므로 포도당을 밀가루 용액내에 첨가하여 실험을 수행하였다. 포도당 첨가할 경우 생성되는 총적정산도(TTA; total titrable acid)가 포도당을 첨가하지 않은 경우에 비하여 적게 증가하였고, pH 감소의 폭도 적었다. 이는 첨가된 포도당이 세포성장을 촉진시키고, 유기산의 생성을 억제하는 것으로 생각된다. 또한, 소다크랙커의 스폰지 발효과정 중에서 밀가루 농도가 증가할수록 생산되는 총적정산도는 증가되고 pH는 감소되었다.

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관상식물 삽목발근에 있어서 NAA, IBA 및 Ethychlozate의 발근촉진효과와 그 생리학적연구 (The Promotive Effect of NAA, IBA and Ethychlozate on Rooting Cuttings of Certain Ornamental Plants and Some Physiological Studies.)

  • 정해준;곽병화
    • 자연과학논문집
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    • 제1권
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    • pp.115-198
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    • 1987
  • The present studies were undertaken to elucidate the influence of auxins, auxin-like substance-ethychlozate ("Figaron"),and pH and sort of rooting media on rooted propagation of certainornamental woody plant cuttings, and to see possible changes in internal compositions characterizing after root-promoting treatment as the cutting stage proceeded. The experimental check-up srevealed and summarized as seen in the following;I. Effect of three different auxin treatments on rooting cuttings: 1) Promotive influence of auxin varied according to different concentration levels, hours of dipping treatment of the auxins, and kind of plants. The greatest effect was obtained for Forsythia ksreana with NAA and IBA, for Ligustrurn obtusifolium var. variegatum with NAA and ethychlozate, for Hydrangea macrophylla, Magnolia kobus, and Magnolia liliflora with NAA, lBA and ethychlozate also. The most effective level of the promotive agents was found 200mg/l for NAA, 1000mg/l for IBA, and 200mg/l for ethychlozate. For Weigela florida and Gardenia jasminoides, range of the most effective level was shown relatively wide spread. 2) NAA was more effective at its optimal level of the rooting agent than ethychiozate for Weigela florida, Viburnum awabuki, Forsythia koreana, Acer palmatum 'Nomura', Bouga invillea glabra, Elaeagnus umbellata, Prunus tomentosa, Ligustrum obtusifolium, Pyracantha coccinea, Cestrum noctu rnum, Hydrangea macrophylla, Codiaeum variegatum, Rhododen dron lateritium, and Ilex crenata var. macrophylla, and yet ethychlozate was found either as equally as effective or more so than NAA for Zebrina pendula, Hibiscus syriacus, Fatshedera lizei, Schefflera arboricola, Campsis grandiflo ra, Ixora chinensis, Euonymus japonica, and Magnolia liliflora. On the contrary, no the auxin effect was noted with Lagerstroemia indica, Trachelospermum asiaticum, and Syringa vulgaris. This probably indicates that these species are genetically different for the auxin response.II. Effect of different pH and sorts of cutting media on rooting cuttings: 1) Bougainvillea showed best in rooting for the number and dry weight at pH 6.5, more with ethychlozate than NAA, while Ligustrum did at pH 5.0 more with NAA than ethychlozate. pH 4.0 medium resulted in the best rooting for Rhododendron with NAA, more than ethychlozate. 2) Use of cutting medium with peat: perlite: vermiculite = 1:1:1 showed to give the greatest rooting percent and dry weight, apart from considering the number of roots. This apparently meant the fact that cutting medium has more to do with root growth than root differentiation. Rhododendron yet showed results with cutting media that use of peat: perlite = 2:1 mixed is more effective on rooting than using peat alone.III. Effect of auxinic treatments on rooting cuttings and change in some cutting compositions: 1) Under the climatic conditions of July having temperature $26.3\pm$$2.4^{\circ}C$for cutting bed, new roots of Magnolia started to show up generally 20 days after the cutting was made, whereas Cestrum did much earlier than that, namely 14 days after. 2) Although total carbohydrate content of Magnolia cuttings showed no marked change without auxin treatment, it did so with the treatment, especially 30 days after the start of cutting. Cestrum cuttings demonstrated a gradual in crease in total carbohydrate content as rooting took place, and the content became reduced more with auxin than with out, just about when rooting proceeded to 14 days after the start of cutting. 3) Magnolia generally showed an increase in total nitrogen content as rooting proceeded more, and Cestrum showed a decrease in total nitrogen of cuttings. The auxin treatment exhibited no pertinent relation with change in plant nitro gen when rooting is promoted with auxin treatment. 4) An abrupt drop of total sugar and reducing sugar was noticed as Magnolia rooting started, and this reduction was parti cularly outstanding with auxin treatment. Starch content also was decreased in the later stage of cutting with auxin treatment, and was rather increased without auxin. Although sugar content soon increased as cutting started with auxin treatment in the case of Cestrum, it became reduced after rooting took place. 5) Total phenol content increased with rooting, and this was especially true when rooting started. This increase was reversed somehow regardless of auxin treatment. A decrease in phenol of Magnolia was found more striking with auxin than without in the later stage of the cutting period. 6)Avena coleoptile test for auxin-like substances presented the physiologically active factor is more in easy-to-root Magnolia liliflora than hard-to-root Magnolia kobus, and the activity of auxin-like substances was much increased with auxin treatment. The increase in the growth promoting substances was markedly pronounced when rooting just started. The active growth substances decreased in the later stage of cutting, and certain inhibitory substances started appearing. Cestrum also showed physiologically similar growth promoting substances accompanying auxin-like active substances if auxin is treated, and some strong inhibitory substances seemed to appear in the later stage of cutting. 7) Mung-bean-rooting test indicated biologically that endogenous growth substances in Magnolia all promoted mung-bean rooting, and activity of the growth substances apparently stimulated mung-bean rooting with auxin more than without. Here auxin treatment seemed to give a rise to an increased activity of endogenous growth substances in cuttings. This activity was found much greater with either NAA or IBA than ethychlozate, and showed its peak of the activity when rooting first started taking place. Certain inhibitory substances for Avena coleoptile growth strongly promoted mung-bean rooting, and it was also much like in the case of Cestrum.

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